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Ern2 (NM_012016) Mouse Tagged ORF Clone
CAT＃: MG222188
- TrueORF®
Ern2 (tGFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2), (10ug)
ORF Plasmid: DDK
"NM_012016" in other vectors (4)
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CNY 8,650.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ern1; Ire1; ire1-beta; Ire1b; mIre1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG222188 representing NM_012016
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAGACCAGTCCAGAGATTTCAACTGTGGTCTCCTCTCGGGTTCCTGCTGCAGCTTGTGACGTTGC TGGGGAAACTCGGTCCACAGGTCCAGTCTGTCAGGCCAGAGAGCCTTCTGTTTGTGTCTACCTTGGACGG AAGTCTCCATGCACTGAACAAGCAGACAGGGGACTTGAAGTGGACAGTGAAGGATGACCCCATCATCCAG GGCCCAATGTATGTCACAGAAATGGCCTTTCTCTCTGACCCAGCTGACGGCAGCCTGTATGTCTTGGGAA CCCAGAAACAACAGGGGCTGATGAAACTGCCATTCACCATCCCAGAGCTGGTTCATGCCTCTCCCTGTCG CAGCTCTGATGGTGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTAGTGGACCCTGAGTCAGGG GAGACTCAGATGACACTGACCACAGAAGGCCTCTCTACTCCTCAACTTTTCATTGGCCGAACACAGTACA CAGTCTCCATGCATGACCTACGGACCCCAGCCCTGCGGTGGAACACAACCTATCGCCGCTACTCAGCACC CCTCCTGAATGGCTCACCCGGGAAATACATGAGCCACCTGACATCCTGTGGGATGGGCCTGCTTCTCACT GTGGATCCAGGAAGTGGAATTGTGCTGTGGACACAGGACCTGGGAGTGCCTGTGACAGGGATTTACACAT GGCACCAGGATGGTCTGCACCAGCTGCCCCATCTCACACTGGCTCGGGACACCCTACATTTCCTTGTCCT CCGCTGGGGCCACATCCGTCTTCCTGCCTCCAGCTACCAAGACACAGCCACCCAGTTCTCTTCCTTAGAC ACCCAGCTTTTGATGACACTGTATGTGGGGAAAGAAGAAGCTGGGTTCTATGTCTCTAAAGCACTGGTCC ACGCTGGGGTGGCCCTCGTGCCCCGTGGACTGACCCTGGCACCCATGGACGGTCCCACAACAGATGAGGT GACACTTCAAGTCTCGGGGGAGCGAGAGGGCTCACCCAGCACTGCTGTCAGATACCCCTCAGGCAGCGTG GCCCTCCCCAGCCAGTGGCTACTCATTGGATACCACGAACCTCCCCCCGTTCTACACACAACCATGCTGC GGGTTCATCCCATCCCAGGGAAAGTGTCTGCTGAGACACGAGCCTCAGAGGACCTCCATGCTCCACCTGT CTTTTTTGAGCTCCTAAACCTGAGGAGGGAGGATCCAGAACTACATCCTGAAGAGAAAGCTTCAGACTCT TATCCAGGGCTGGGATCCCAAGACCTGCTAGCAGCTACCTTCACTGCCATCCTCCTGGGAGCCTGGGTCC TCTACCTGATGAGGCAGCAGCAGCAGAGCCCTTCAGCACCTGCTGGCCCTCCTGACCTCTCACAGGATGC TCAGGGACAGCTCTCAAGGGACATCTTACAGGACCAGAGGAGATTTCAAAGCCCCTCAGAGCCAGCCCAG CCACCTCATGACCCTGAAGGGCAGCCCACCGTGGTGGGGAAGATTTCCTTCAACCCCAAGGATGTGCTGG GCCGTGGGGCAGGAGGGACTTTTGTTTTCCGAGGACAGTTTGAGGGGCGGGCAGTAGCTGTGAAGAGGCT CCTCCGAGAGTGCTTTGGCTTGGTTCGGCGAGAGGTGCAGCTGCTGCAAGAGTCTGACCGGCACCCCAAC GTGCTTCGCTACTTCTGCACTGAGCATGGTCCCCAGTTCCACTACATTGCCTTGGAGCTCTGCCAGGCCT CCTTGCAAGAGTACGTGGAGAGCCCAGACCTAGATCGCTGGGGCCTGGAGCCCACCACAGTGCTGCAGCA GATGATGTCCGGCCTGGCCCATCTACATTCCTTACACATAGTACACCGTGACCTAAAGCCAGCCAACATT CTCATGGCTGGGCCTGACAGCCAGGGCCAAGGCAGAGTGGTCATCTCTGACTTCGGCCTCTGCAAGAAGC TACCTGTTGGCCGTTGTAGCTTCAGTCTCCATTCTGGTATTCCCGGCACAGAAGGCTGGATGGCACCAGA GCTCCTGCAGCTCCCCCCAGACAGCCCGACCAGCGCTGTGGATATCTTCTCCGCAGGCTGCGTATTTTAT TACGTGCTTTCCGGTGGCAGCCACCCCTTTGGAGAGAGTCTCTACCGACAGGCCAACATCCTCTCAGGGG ATCCCTGTCTGGCTCAGCTGCAGGAAGAGACCCATGACAAGGTTGTGGCATTGGACTTAGTGAGAGCCAT GCTGAGCTTGCTGCCCCAGGATCGCCCCTCGGCAGGATGGGTACTGGCTCACCCCCTCTTTTGGAGCAGA GCTAAGGAGCTCCAGTTCTTCCAGGATGTCAGTGACTGGTTGGAGAAGGAACCAGATCAGGGGCCTCTCG TGTCGGCGCTGGAGGCAGGAAGCTACAAGGTGGTCCGGGAAGACTGGCACAAGCACATCTCAGCCCCTCT GCAGGCAGATCTGAAAAGGTTCCGCTCATACAAAGGGACATCAGTTCGAGACCTGCTCCGGGCCATGAGG AACAAGAAGCACCACTACAGGGAGCTCCCAGCTGAGGTGCGCCAGACACTAGGCCAACTTCCTGCTGGCT TCATTCAGTACTTCACACAGCGATTCCCCAGGCTGTTGCTGCACACTCACCGTGCCATGAGGACCTGCGC CTCTGAGAGCCTCTTCCTGCCCTACTACCCACCAGCTCTGGAAGCCAGGAGGCCAGATGCCACAAAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222188 representing NM_012016
Red=Cloning site Green=Tags(s) MARPVQRFQLWSPLGFLLQLVTLLGKLGPQVQSVRPESLLFVSTLDGSLHALNKQTGDLKWTVKDDPIIQ GPMYVTEMAFLSDPADGSLYVLGTQKQQGLMKLPFTIPELVHASPCRSSDGVFYTGRKQDAWFVVDPESG ETQMTLTTEGLSTPQLFIGRTQYTVSMHDLRTPALRWNTTYRRYSAPLLNGSPGKYMSHLTSCGMGLLLT VDPGSGIVLWTQDLGVPVTGIYTWHQDGLHQLPHLTLARDTLHFLVLRWGHIRLPASSYQDTATQFSSLD TQLLMTLYVGKEEAGFYVSKALVHAGVALVPRGLTLAPMDGPTTDEVTLQVSGEREGSPSTAVRYPSGSV ALPSQWLLIGYHEPPPVLHTTMLRVHPIPGKVSAETRASEDLHAPPVFFELLNLRREDPELHPEEKASDS YPGLGSQDLLAATFTAILLGAWVLYLMRQQQQSPSAPAGPPDLSQDAQGQLSRDILQDQRRFQSPSEPAQ PPHDPEGQPTVVGKISFNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPN VLRYFCTEHGPQFHYIALELCQASLQEYVESPDLDRWGLEPTTVLQQMMSGLAHLHSLHIVHRDLKPANI LMAGPDSQGQGRVVISDFGLCKKLPVGRCSFSLHSGIPGTEGWMAPELLQLPPDSPTSAVDIFSAGCVFY YVLSGGSHPFGESLYRQANILSGDPCLAQLQEETHDKVVALDLVRAMLSLLPQDRPSAGWVLAHPLFWSR AKELQFFQDVSDWLEKEPDQGPLVSALEAGSYKVVREDWHKHISAPLQADLKRFRSYKGTSVRDLLRAMR NKKHHYRELPAEVRQTLGQLPAGFIQYFTQRFPRLLLHTHRAMRTCASESLFLPYYPPALEARRPDATKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012016 |
| ORF Size | 2733 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012016.2, NP_036146.2 |
| RefSeq Size | 2973 bp |
| RefSeq ORF | 2736 bp |
| Locus ID | 26918 |
| UniProt ID | Q9Z2E3 |
| Gene Summary | Role in expression of the DDIT3 transcription factor, required for the unfolded-protein response, growth arrest and apoptosis. Has no effect on 28S ribosomal RNA cleavage, unlike the corresponding human protein.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222523 | Ern2 (untagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2), (10ug) |
CNY 11,690.00 |
|
| MR222188 | Ern2 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2) |
CNY 6,672.00 |
|
| MR222188L3 | Lenti ORF clone of Ern2 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2) |
CNY 9,072.00 |
|
| MR222188L4 | Lenti ORF clone of Ern2 (mGFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 2 (Ern2) |
CNY 9,790.00 |
