Sp5 (NM_022435) Mouse Tagged ORF Clone
CAT#: MG222085
- TrueORF®
Sp5 (tGFP-tagged) - Mouse trans-acting transcription factor 5 (Sp5), (10ug)
ORF Plasmid: DDK
"NM_022435" in other vectors (4)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MGC144801; MGC144802 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222085 representing NM_022435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCTGTGGCCGTCCTCCGGAACGACTCACTGCAGGCCTTCCTCCAGGACCGCACCCCCAGCGCCT CCCCGGACCTGGGCAAGCACTCGCCCCTGGCGCTGCTGGCCGCCACCTGTAGCCGGATCGGCCAGCCCGG CGCTGCGGCGGCACCCGACTTCCTTCAGGTGCCCTATGACCCAGCGCTGGGTTCACCCTCCAGACTTTTC CACCCTTGGACTGCCGACATGCCCGCGCACTCGCCAGGCGCCCTGCCGCCCCCACACCCCAGCCTGGGGC TGACGCCGCAGAAAACACACCTGCAGCCGTCCTTCGGGGCAGCCCACGAGCTCCCGCTCACGCCCCCCGC GGATCCGTCGTACCCTTACGAGTTCTCGCCGGTCAAGATGCTGCCCTCGAGCATGGCTGCTCTGCCTGCC AGCTGCGCGCCCGCCTACGTGCCCTACGCCGCGCAGGCCGCGTTGCCCCCGGGCTACTCCAACCTGCTGC CCCCGCCGCCGCCACCGCCTCCACCGCCCACCTGCCGCCAGTTATCCCCCGCCCCGGCTCCGGACGACCT CCCCTGGTGGAGCATCCCGCAATCGGGCGCGGGGCCGGGGAGCTCCGGGGTTCCAGGGACCAGCCTCTCC AGCGCCTGTGCCGGACCTCCCCACGCTCCCCGCTTCCCTGCCTCAGCCGCCGCTGCTGCAGCGGCGGCTG CTGCCCTGCAACGGGGTCTAGTGTTGGGCCCGTCGGACTTTGCACAGTACCAGAGCCAGATCGCCGCGCT GCTGCAGACCAAGGCCCCCCTGGCGGCCACGGCCAGGAGGTGCCGCCGCTGCCGCTGCCCCAACTGCCAG GCGGCTGGCGGTGCCCCCGAGGCGGAACCGGGCAAAAAGAAGCAACACGTGTGCCACGTGCCAGGCTGTG GCAAGGTGTACGGCAAAACGTCGCACCTGAAGGCGCACCTGCGCTGGCACACGGGCGAGCGGCCCTTCGT GTGCAACTGGCTCTTCTGCGGCAAGAGCTTCACGCGCTCGGACGAGCTGCAGCGGCACCTGCGGACTCAC ACGGGCGAGAAGCGCTTCGCTTGTCCCGAGTGCGGCAAACGCTTCATGCGAAGCGATCACCTCGCCAAGC ACGTGAAGACGCACCAAAATAAGAAGCTCAAAGTCGCTGAGGCCGGGGTGAAGCGGGAGAATCCGCGGGA CCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222085 representing NM_022435
Red=Cloning site Green=Tags(s) MAAVAVLRNDSLQAFLQDRTPSASPDLGKHSPLALLAATCSRIGQPGAAAAPDFLQVPYDPALGSPSRLF HPWTADMPAHSPGALPPPHPSLGLTPQKTHLQPSFGAAHELPLTPPADPSYPYEFSPVKMLPSSMAALPA SCAPAYVPYAAQAALPPGYSNLLPPPPPPPPPPTCRQLSPAPAPDDLPWWSIPQSGAGPGSSGVPGTSLS SACAGPPHAPRFPASAAAAAAAAAALQRGLVLGPSDFAQYQSQIAALLQTKAPLAATARRCRRCRCPNCQ AAGGAPEAEPGKKKQHVCHVPGCGKVYGKTSHLKAHLRWHTGERPFVCNWLFCGKSFTRSDELQRHLRTH TGEKRFACPECGKRFMRSDHLAKHVKTHQNKKLKVAEAGVKRENPRDL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_022435 |
ORF Size | 1194 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022435.2, NP_071880.1 |
RefSeq Size | 2097 bp |
RefSeq ORF | 1197 bp |
Locus ID | 64406 |
UniProt ID | Q9JHX2 |
Gene Summary | Binds to GC boxes promoters elements. Probable transcriptional activator that has a role in the coordination of changes in transcription required to generate pattern in the developing embryo.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210227 | Sp5 (untagged) - Mouse trans-acting transcription factor 5 (Sp5), (10ug) |
CNY 3,990.00 |
|
MR222085 | Sp5 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 5 (Sp5) |
CNY 3,656.00 |
|
MR222085L3 | Lenti ORF clone of Sp5 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 5 (Sp5) |
CNY 4,750.00 |
|
MR222085L4 | Lenti ORF clone of Sp5 (mGFP-tagged) - Mouse trans-acting transcription factor 5 (Sp5) |
CNY 4,750.00 |