Gal3st1 (NM_016922) Mouse Tagged ORF Clone
CAT#: MG221922
- TrueORF®
Gal3st1 (tGFP-tagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_016922" in other vectors (5)
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CNY 3,140.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Cst; Gcst |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG221922 representing NM_016922
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTCTGCTGCCAAAGAAGCCCTGCAAGTCCAAGGCCAAGGGGTTACTGCTGGGAGCTCTCTTCACCA GTTTCCTATTGCTGCTGTACTCCTATGTGGTACCTCCACTGTATCCCAACATGGCCTTCACGACCTCAGA GGCTGCAGCACCCTGCTCCCCTATTCCCAATGAGCCAGTGGCAGCCACTCCCGCCAACGGCTCAGCAGGA GGGTGTCAACCTCGGCGAGATATTGTGTTCATGAAGACGCACAAGACCGCCAGCAGCACACTGCTCAACA TCCTGTTCCGCTTCGGCCAGAAGCACGAGCTCAAGTTCGCTTTCCCCAATGGCCGAAACGACTTCCACTA CCCGTCGTACTTTGCACGAAGCCTGGTGCAGGACTACCGGCCTGGGGCGTGCTTCAACATCATCTGCAAC CACATGCGCTTCCACTATGAGGAAGTGCGCGGGCTGGTGCGGCCGGGTGCCACCTTCATCACCGTCATCC GCGACCCTGCTCGTCTCTTCGAGTCTTCCTTCCACTACTTTGGATCCGTGGTGCCGCTCACCTGGAAGCT GTCGAGCCGCGACAAGCTGGCCGAGTTCCTGCAGGACCCCGATCGCTACTACGACCCGAGCAGCTACAAC GCGCACTACCTCCGCAACCTGCTCTTCTTCGATCTGGGCTATGATAGTAGCCTGGACCCGGCCAGCCCGC GCGTGCAGGAGCACATCCTGGAGGTGGAGCGCCGCTTCCACTTGGTGCTACTGCAGGAGTACTTCGACGA ATCCCTGGTGCTGCTCCGAGAGCTGCTGTGCTGGGACCTGGAGGACGTGCTGTATTTCAAGCTCAATGCG CGGCGCGACTCGCCGGTTCCGCGGCTCTCTGGCGAGCTGTACCGCCGAGCCACCGCCTGGAACCTGCTGG ACGTGCGCCTCTACCGTCACTTCAACGCCAGCTTCTGGCGAAAGGTGGAAGCTTTCGGGCGTGAGCGCAT GGCGCGCGAGGTAGCCGAGCTGCGCCAAGCGAACGAGCACATGCGCCACATCTGCATAGATGGCGGCCAA GCAGTTGGTGCTGAGGCCATCCAGGACTCGGCCATGCAGCCCTGGCAACCCCTGGGCATCAAGTCCATCC TGGGTTACAACCTCAAGAAGAGCATCGGGCCACAACACGAGCAGCTCTGCCGGCGCATGCTCACGCCCGA AATCCAGTACCTGTCTGACCTCGGTGCCAATCTCTGGGTCACCAAGCTCTGGAAGTTCCTTAGGGACTTT CTAAGGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221922 representing NM_016922
Red=Cloning site Green=Tags(s) MTLLPKKPCKSKAKGLLLGALFTSFLLLLYSYVVPPLYPNMAFTTSEAAAPCSPIPNEPVAATPANGSAG GCQPRRDIVFMKTHKTASSTLLNILFRFGQKHELKFAFPNGRNDFHYPSYFARSLVQDYRPGACFNIICN HMRFHYEEVRGLVRPGATFITVIRDPARLFESSFHYFGSVVPLTWKLSSRDKLAEFLQDPDRYYDPSSYN AHYLRNLLFFDLGYDSSLDPASPRVQEHILEVERRFHLVLLQEYFDESLVLLRELLCWDLEDVLYFKLNA RRDSPVPRLSGELYRRATAWNLLDVRLYRHFNASFWRKVEAFGRERMAREVAELRQANEHMRHICIDGGQ AVGAEAIQDSAMQPWQPLGIKSILGYNLKKSIGPQHEQLCRRMLTPEIQYLSDLGANLWVTKLWKFLRDF LRW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016922 |
ORF Size | 1269 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016922.3, NP_058618.2 |
RefSeq Size | 1969 bp |
RefSeq ORF | 1272 bp |
Locus ID | 53897 |
UniProt ID | Q9JHE4 |
Gene Summary | Catalyzes the sulfation of membrane glycolipids. Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety. Catalyzes the synthesis of HSO3-3-galactosylceramide (sulfatide), a major lipid component of the myelin sheath and of HSO3-3-monogalactosylalkylacylglycerol (seminolipid), present in spermatocytes. Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204018 | Gal3st1 (untagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1), transcript variant 1, (10ug) |
CNY 5,488.00 |
|
MG206735 | Gal3st1 (tGFP-tagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1) |
CNY 3,140.00 |
|
MR221922 | Gal3st1 (Myc-DDK-tagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1), transcript variant 1 |
CNY 5,488.00 |
|
MR221922L3 | Lenti ORF clone of Gal3st1 (Myc-DDK-tagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1), transcript variant 1 |
CNY 4,750.00 |
|
MR221922L4 | Lenti ORF clone of Gal3st1 (mGFP-tagged) - Mouse galactose-3-O-sulfotransferase 1 (Gal3st1), transcript variant 1 |
CNY 4,750.00 |
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