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Lrrfip1 (NM_008515) Mouse Tagged ORF Clone
CAT＃: MG221885
- TrueORF®
Lrrfip1 (tGFP-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_008515" in other vectors (4)
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CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU024550; Fliiap1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221885 representing NM_008515
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGCCCAGAGGGCGCGCAAAACAAGGAAATCGACTGTCTGAGTCCAGAGGCGCAAAGACTGGCGG AGGCCAGGCTCGCAGCGAAGCGTGCAGCCCGAGCCGAGGCTCGAGAGATCCGGATGAAGGAGCTGGAACG GCAGCAGAAAGAGATCTATCAAGTCCAGAAGAAATATTATGGGCTGGATACAAAATGGGGCGACATCGAG CAGTGGATGGAAGACAGCGAGCGCTACTCTCGTAGATTCCGGAGAAACACGTCGGCTTCTGACGAAGACG AGCGCTTGTCCGTGGGTAGTCGCGGCAGCCTAAGGACTAACGGTTATGACGGAGACTATTGTGGATCCCA GTCCCTAAGTAGAAGATCTGGCAGGGGACTCTCCTGCTCCAACCTTGGCCTTCCCAGCAGTGGCCTTGCT TCCAAACCCCTGTCTACCCAGAATGGATCCCGGGCGTCCATGCTGGATGAGAGCAGCCTCTATGGGGCTC GAAGGGGAAGCGCCTGTGGTTCTCGAGCACCCTCAGAATATGGCAGCCACCTCAACTCCAGCTCCCGGGC CTCTTCTAGGGCCAGCTCTGCAAGGGCCAGTCCTGTGGTAGAAGAAAGACCAGACAAAGACTTTGCTGAG AAGGGCTCCCGTAACATGCCTAGCCTGTCTGCAGCCACACTGGCATCTCTGGGTGGGACTTCCTCCCGGA GAGGGAGTGGAGATACCTCCATCTCCATGGACACCGAAGCATCCATCAGGGAAATCAAGGAACTTAATGA GTTAAAGGACCAGATTCAGGATGTAGAAGGCAAATACATGCAGGGACTGAAAGAGATGAAGGACTCGCTG GCCGAAGTTGAGGAGAAGTACAAGAAAGCTATGGTCTCCAACGCCCAGCTAGACAATGAGAAGACCAACT TCATGTACCAGGTGGACACACTGAAGGACATGCTGCTGGAGCTGGAAGAGCAGCTGGCCGAGTCTCAGCG GCAATACGAGGAGAAGAACAAGGAGTTCGAGAGGGAGAAGCATGCCCACAGCATCCTGCAGTTCCAGTTT GCCGAAGTGAAAGAGGCCCTGCGGCAAAGGGAAGAGATGCTCGAGGAAATCCGACAGCTGCAGCAGAAAC AGGCGGGTTTTATCAGGGAGATTTCTGATCTTCAGGAAACGATAGAATGGAAAGACAAAAAGATAGGGGC ATTAGAAAGACAGAAAGAGTTCTTTGATTCCATAAGGAGCGAACGAGATGACCTTAGAGAAGAAACAGTC AAGCTGAAGGAGGAGTTGAAGAAACATGGAATAATCCTAAATTCAGAAATAGCCACCAACGGAGAGACTT CAGACACAGTAAATGACGTTGGGTATCAAGCCCCTACGAAGATAACAAAAGAAGAGCTGAATGCCCTCAA GTCGGCCGGGGAGGGGACCCTAGACGTTAGGTTGAAAAAGCTCATTGATGAACGGGAATGTCTGTTGGAA CAGATTAAGAAGCTCAAAGGGCAGCTGGAGGGAAGACAGAAGAACAACAAGCTGGACCTTCTTCGAGCCG AGGACGGTATCTTGGAGAATGGGACAGACGCGCATGTCATGGATCTGCAAAGAGATGCCAACAGACAGAT CAGCGACCTCAAATTTAAACTTGCAAAATCTGAGCAAGAGATAACTGCCTTAGAACAAAATGTAATAAGG TTAGAGAGCCAAGTAACGCGCTATCGATCAGCCGCCGAAAATGCAGAAAAAATAGAAGATGAACTAAAGG CAGAAAAACGGAAGCTCCAGAGAGAGCTTCGCTCTGCACTGGACAAAACAGAAGAGCTCGAGGTGAGCAA CGGCCACCTCGTAAAGCGACTGGAAAAGATGAAAGCCAACCGCAGCGCTCTCTTGTCCCAGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221885 representing NM_008515
Red=Cloning site Green=Tags(s) MTSPEGAQNKEIDCLSPEAQRLAEARLAAKRAARAEAREIRMKELERQQKEIYQVQKKYYGLDTKWGDIE QWMEDSERYSRRFRRNTSASDEDERLSVGSRGSLRTNGYDGDYCGSQSLSRRSGRGLSCSNLGLPSSGLA SKPLSTQNGSRASMLDESSLYGARRGSACGSRAPSEYGSHLNSSSRASSRASSARASPVVEERPDKDFAE KGSRNMPSLSAATLASLGGTSSRRGSGDTSISMDTEASIREIKELNELKDQIQDVEGKYMQGLKEMKDSL AEVEEKYKKAMVSNAQLDNEKTNFMYQVDTLKDMLLELEEQLAESQRQYEEKNKEFEREKHAHSILQFQF AEVKEALRQREEMLEEIRQLQQKQAGFIREISDLQETIEWKDKKIGALERQKEFFDSIRSERDDLREETV KLKEELKKHGIILNSEIATNGETSDTVNDVGYQAPTKITKEELNALKSAGEGTLDVRLKKLIDERECLLE QIKKLKGQLEGRQKNNKLDLLRAEDGILENGTDAHVMDLQRDANRQISDLKFKLAKSEQEITALEQNVIR LESQVTRYRSAAENAEKIEDELKAEKRKLQRELRSALDKTEELEVSNGHLVKRLEKMKANRSALLSQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008515 |
| ORF Size | 1884 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008515.4, NP_032541.1 |
| RefSeq Size | 2850 bp |
| RefSeq ORF | 1887 bp |
| Locus ID | 16978 |
| UniProt ID | Q3UZ39 |
| Gene Summary | Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA (By similarity). Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219804 | Lrrfip1 (untagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 2, (10ug) |
CNY 7,980.00 |
|
| MR221885 | Lrrfip1 (Myc-DDK-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 2 |
CNY 7,016.00 |
|
| MR221885L3 | Lenti ORF clone of Lrrfip1 (Myc-DDK-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 2 |
CNY 8,170.00 |
|
| MR221885L4 | Lenti ORF clone of Lrrfip1 (mGFP-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 2 |
CNY 8,170.00 |
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