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Lrrfip1 (NM_001111311) Mouse Tagged ORF Clone
CAT＃: MG221884
- TrueORF®
Lrrfip1 (tGFP-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001111311" in other vectors (6)
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CNY 9,608.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU024550; Fliiap1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221884 representing NM_001111311
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAGCCCAGAGGGCGCGCAAAACAAGGAAATCGACTGTCTGAGTCCAGAGGCGCAAAGACTGGCGG AGGCCAGGCTCGCAGCGAAGCGTGCAGCCCGAGCCGAGGCTCGAGAGATCCGGATGAAGGAGCTGGAACG GCAGCAGAAAGAGGTAGAAGAAAGACCAGACAAAGACTTTGCTGAGAAGGGCTCCCGTAACATGCCTAGC CTGTCTGCAGCCACACTGGCATCTCTGGGTGGGACTTCCTCCCGGAGAGGGAGTGGAGATACCTCCATCT CCATGGACACCGAAGCATCCATCAGGGAAATCAAGGACTCGCTGGCCGAAGTTGAGGAGAAGTACAAGAA AGCTATGGTCTCCAACGCCCAGCTAGACAATGAGAAGACCAACTTCATGTACCAGGTGGACACACTGAAG GACATGCTGCTGGAGCTGGAAGAGCAGCTGGCCGAGTCTCAGCGGCAATACGAGGAGAAGAACAAGGAGT TCGAGAGGGAGAAGCATGCCCACAGCATCCTGCAGTTCCAGTTTGCCGAAGTGAAAGAGGCCCTGCGGCA AAGGGAAGAGATGCTCGAGAAACATGGAATAATCCTAAATTCAGAAATAGCCACCAACGGAGAGACTTCA GACACAGTAAATGACGTTGGGTATCAAGCCCCTACGAAGATAACAAAAGAAGAGCTGAATGCCCTCAAGT CGGCCGGGGAGGGGACCCTAGGAAAAGCCAAAGAAGTGGAGGTGAAAAAGGAAATTGTGGAGAAAGTGGG GCAAAGAGAAACCTTACAGAATTCTGAGCAAGAACAGCCCAAACCCAACACAGGAAAGGATTGTGTGGAC AGAGGGGTGTCACATCCTGGTGAGAAGGCTGAGAACCAGAGACCCGCTGAAGACAGTGCCCTGTCCCCGG GACCACTAGCAGGGGCCAAATGTGAGCAACAGGTTCAGAGCCAAGATCAAGAGAATACTTCTGACCTCAA AAACTCAGAGCAGATCGAGTCACACAAGGTCACAAACAAGTCAGATAGTAGGGCCAGTAACTCCCCTGAA CAGTCCAGCTGCCTGGAAGGTCTAGACAGTGAAGTGCCAGGGCCCACTGAGGATTTGAAAACAGATTTGG GGAAAGGTAGCTTTGAACCATGTCCAGACTACATTTTGGGGCAAACAGCTGAAATTGACAAGGTGACCTG TACAGACTCAAGAGGCACAGGTGGAAACCAAAGGGAGGATGAGGTACAGGCAGGGGACACCACAGTTGAG GACCAAGTAGGGACAGTGGCTTCTGGACCTGCAAAGCAAAGCAAAGGCACCGAGAATCATGGTGAAAGTT GTCTGAAGGATGGGCTGGGACAGAGCTCAGAGAGAGAACTCACCCAAGAAGTAGCTGAGCCTGAGGAAGC CATCGTTCAGATCCCACAGGCCGGTGGAGAGAACACTATTACCAAGGCAGATGATGCCGAAGGAAGGGAT GAGAAGCCCATCCAGGCAGAAGCCCAGGCAAGCCCTGGAGCTCCCATAAATCAGAGCGGCCATCAGGACA CAACAGGACCAGGCAGCACCGATGCCCAGCGCACACCACCTCATGCAAAAGAGCGCAAGAAACAAGGGAA GAGCGAGCAGCAGGCAGAGGCCTTGGATTCACCGCAGAAGAAGACGAAGAACAAGAAGAAGAAGAACAAG AAGAAGAAAGCTGCAACTCCCGCGGAAACCTGCAGAGATGCCAATGAAGAGTTGAACTGTCAGGACCCAG ATGTGGGTGACATGGAGGAGGAAGAGCGGCTCCAGGTCACTGACAAGAAACAGGCATCAGGAAGCCCAGA GCAGAAAATCAGAGCGGGAAGCAGAGAACCTGTCGAAGATCCTCAAAGTGGGTCGAGTGGAAAACAGAAC AAAGTGGAAGAGGATGGTCCCACGGAAGGTCCCACAGACATCTTGGATCAGAACAGTCCCCAGTGTGAAG ACAGGGAGATCTCCCCAGTGGGAGAGAAAGGTCCCCAATGCGATACCTCTCAGATAGGTAGTGAAGAGGG ACATGTGACCTCCCAACACGGTGGTCAGGCAGTTGAGAACCACAACCTAGATAACAGTGACCTGTCAGGG CAGCTGGAGGGCTTCAATTCAGAAAGTGGAGGGCAGGCAAGAGAGGAGGTTGGGAATAGCAAGAGTAAGG AGGACTGCACCATGTCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221884 representing NM_001111311
Red=Cloning site Green=Tags(s) MTSPEGAQNKEIDCLSPEAQRLAEARLAAKRAARAEAREIRMKELERQQKEVEERPDKDFAEKGSRNMPS LSAATLASLGGTSSRRGSGDTSISMDTEASIREIKDSLAEVEEKYKKAMVSNAQLDNEKTNFMYQVDTLK DMLLELEEQLAESQRQYEEKNKEFEREKHAHSILQFQFAEVKEALRQREEMLEKHGIILNSEIATNGETS DTVNDVGYQAPTKITKEELNALKSAGEGTLGKAKEVEVKKEIVEKVGQRETLQNSEQEQPKPNTGKDCVD RGVSHPGEKAENQRPAEDSALSPGPLAGAKCEQQVQSQDQENTSDLKNSEQIESHKVTNKSDSRASNSPE QSSCLEGLDSEVPGPTEDLKTDLGKGSFEPCPDYILGQTAEIDKVTCTDSRGTGGNQREDEVQAGDTTVE DQVGTVASGPAKQSKGTENHGESCLKDGLGQSSERELTQEVAEPEEAIVQIPQAGGENTITKADDAEGRD EKPIQAEAQASPGAPINQSGHQDTTGPGSTDAQRTPPHAKERKKQGKSEQQAEALDSPQKKTKNKKKKNK KKKAATPAETCRDANEELNCQDPDVGDMEEEERLQVTDKKQASGSPEQKIRAGSREPVEDPQSGSSGKQN KVEEDGPTEGPTDILDQNSPQCEDREISPVGEKGPQCDTSQIGSEEGHVTSQHGGQAVENHNLDNSDLSG QLEGFNSESGGQAREEVGNSKSKEDCTMS TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001111311 |
| ORF Size | 2187 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001111311.1, NP_001104781.1 |
| RefSeq Size | 3662 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 16978 |
| UniProt ID | Q3UZ39 |
| Gene Summary | Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA (By similarity). Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221155 | Lrrfip1 (untagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1, (10ug) |
CNY 9,500.00 |
|
| MR221884 | Lrrfip1 (Myc-DDK-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1 |
CNY 8,008.00 |
|
| MR221884L1 | Lenti ORF clone of Lrrfip1 (Myc-DDK-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1 |
CNY 10,408.00 |
|
| MR221884L2 | Lenti ORF clone of Lrrfip1 (mGFP-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1 |
CNY 9,220.00 |
|
| MR221884L3 | Lenti ORF clone of Lrrfip1 (Myc-DDK-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1 |
CNY 9,220.00 |
|
| MR221884L4 | Lenti ORF clone of Lrrfip1 (mGFP-tagged) - Mouse leucine rich repeat (in FLII) interacting protein 1 (Lrrfip1), transcript variant 1 |
CNY 9,220.00 |
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