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Gnai1 (NM_010305) Mouse Tagged ORF Clone
CAT＃: MG221647
- TrueORF®
Gnai1 (tGFP-tagged) - Mouse guanine nucleotide binding protein (G protein) alpha inhibiting 1 (Gnai1), (10ug)
ORF Plasmid: DDK
"NM_010305" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AU046200; Gialpha1; Gnai-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221647 representing NM_010305
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGCACATTGAGCGCTGAGGACAAGGCGGCCGTGGAGCGCAGCAAGATGATCGACCGCAACCTCC GGGAGGACGGCGAGAAGGCGGCGCGGGAGGTCAAGCTGCTGCTGCTGGGTGCTGGGGAATCTGGAAAGAG TACCATTGTGAAGCAGATGAAGATTATCCACGAAGCCGGCTACTCGGAAGAGGAGTGTAAGCAGTACAAG GCAGTGGTCTACAGCAACACTATCCAGTCCATCATTGCCATCATTAGAGCCATGGGGAGGTTGAAAATCG ACTTCGGAGACTCTGCTCGGGCGGATGATGCTCGCCAACTTTTCGTGCTTGCTGGGGCTGCAGAAGAAGG CTTTATGACTGCAGAGCTCGCCGGTGTCATAAAGAGACTGTGGAAAGACAGTGGTGTGCAAGCCTGCTTC AACAGATCCCGGGAGTACCAGCTGAACGATTCGGCAGCGTACTATCTGAATGACTTGGACAGAATAGCAC AGCCAAATTACATCCCAACTCAGCAGGATGTCCTCAGAACCAGAGTGAAGACCACAGGGATTGTGGAAAC CCACTTTACCTTCAAAGATCTTCATTTTAAAATGTTTGACGTGGGAGGTCAGAGGTCAGAGCGGAAGAAG TGGATCCACTGCTTTGAAGGGGTGACCGCCATCATCTTCTGTGTGGCCCTGAGTGACTATGACCTGGTTC TTGCTGAAGATGAAGAAATGAACCGTATGCACGAGAGCATGAAGCTGTTCGATAGCATCTGTAACAACAA GTGGTTTACAGACACGTCCATCATCCTTTTCCTCAACAAGAAGGACCTCTTCGAAGAAAAAATAAAAAAG AGCCCCCTCACGATATGCTACCCAGAATATGCAGGCTCAAACACATATGAAGAAGCGGCCGCGTATATTC AGTGTCAGTTTGAAGACCTCAATAAAAGGAAGGACACAAAGGAAATTTACACCCACTTCACGTGCGCCAC AGATACGAAGAACGTGCAGTTCGTGTTCGATGCTGTAACAGACGTCATCATAAAGAATAACCTAAAAGAC TGTGGTCTCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221647 representing NM_010305
Red=Cloning site Green=Tags(s) MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYK AVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACF NRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKK WIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKK SPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKD CGLF TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010305 |
| ORF Size | 1062 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010305.1, NP_034435.1 |
| RefSeq Size | 3193 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 14677 |
| UniProt ID | B2RSH2 |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (By similarity). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (By similarity). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis. Required for cortical dynein-dynactin complex recruitment during metaphase (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208563 | Gnai1 (untagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1), (10ug) |
CNY 5,488.00 |
|
| MR221647 | Gnai1 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1) |
CNY 5,488.00 |
|
| MR221647L3 | Lenti ORF clone of Gnai1 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1) |
CNY 5,890.00 |
|
| MR221647L4 | Lenti ORF clone of Gnai1 (mGFP-tagged) - Mouse guanine nucleotide binding protein (G protein), alpha inhibiting 1 (Gnai1) |
CNY 5,890.00 |
