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Nfasc (NM_001160316) Mouse Tagged ORF Clone
CAT＃: MG221047
- TrueORF®
Nfasc (tGFP-tagged) - Mouse neurofascin (Nfasc) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001160316" in other vectors (4)
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CNY 13,110.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA387016; D430023G06Rik; mKIAA0756; NF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221047 representing NM_001160316
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATCGAGATTCCGATGGACCTGACCCAACCCCCAACTATCACCAAGCAGTCCGTGAAGGACCACATCGT GGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCCCCCAGTTTTCACTGGACT CGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGATCTGGGACCTTGG TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA ATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACCCCCCACCCGGCCTCCCGT CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAACGCGCGC TTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCCTCACCAACAACCCCTATA ATGACTCGTCCTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCAGAAAGAACGCCCAGCTT CATGTATCCCCAGGGCACATCGAGCAGTCAGATGGTTCTCCGTGGCATGGACCTGCTGCTGGAATGCATT GCCTCTGGCGTCCCAACACCAGACATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTAACAAGGCCA AGTTCGAGAACTTTAATAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCATACTGG CTGGATGAGCCCAAGAACCTGATCCTGGCTCCTGGGGAAGATGGGAGGCTGGTATGCCGAGCCAATGGGA ACCCGAAGCCGACCGTGCAGTGGATGGTGAATGGAGAGCCTTTACAATCGGCACCACCCAATCCCAACCG TGAGGTAGCTGGAGACACTATCATCTTCCGGGATACTCAGATCAGCAGCAGGGCAGTGTACCAATGTAAT ACATCCAATGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTGTTAGATGTACCCCCTCGGATGC TGTCTGCCCGCAACCAGCTCATCAGGGTGATCCTTTATAACCGGACACGGCTGGACTGTCCGTTCTTTGG GTCTCCCATCCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGCGGTAACTACCAC GTCTACGAAAACGGCAGTCTAGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG CCACCAACATCCTGGGCAAAGCCGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG GATGCCCGAGGACCAGGTGGCCAAGAGGGGCACCACGGTGCAGCTGGAGTGCCGCGTGAAACATGACCCC TCCTTGAAGCTCACAGTCTCCTGGCTGAAGGACGATGAGCCACTCTACATTGGAAACAGGATGAAGAAGG AAGATGACTCCCTGACGATCTTCGGAGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTATGGCCAGCAC CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGCTGATCAGGCCACTCCAACTAACCGT TTGGCTGCCCTACCCAAAGGGCGACCAGACCGACCCAGGGACCTGGAGCTCACTGACCTGGCTGAGAGGA GTGTGAGGCTGACCTGGATCCCAGGGGATGACAACAACAGCCCTATCACAGACTACGTCGTTCAGTTTGA AGAGGACCAGTTCCAACCAGGGGTGTGGCATGACCACTCCAGGTTCCCAGGCAGCGTCAACTCAGCCGTC CTCCATCTGTCCCCATATGTCAACTACCAATTCAGAGTCATCGCTGTCAACGAGGTTGGGAGCAGCCACC CCAGCCTTCCATCCGAGCGGTACCGAACCAGTGGGGCACCCCCTGAATCTAATCCCAGTGATGTGAAGGG CGAAGGGACAAGAAAGAACAATATGGAGATCACGTGGACGCCTATGAATGCTACCTCTGCCTTTGGCCCC AACCTACGCTACATTGTCAAGTGGCGACGGAGAGAGACCCGAGAGACTTGGAACAATGTCACAGTGTGGG GCTCTCGCTACGTGGTGGGGCAGACGCCTGTCTACGTTCCCTATGAGATCCGAGTCCAGGCTGAAAATGA CTTTGGGAAAGGCCCCGAGCCTGACACCATCATTGGGTACTCCGGAGAAGATTATCCCAGGGCTGCGCCC ACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTTCAGTGGAACCGAGTCTACTCTGACA CGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGGAAAGCAGTTTGCTGAAGAACCTGTG GGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGTGACCGTCCCCGGGGCGTGGTGGCCCGCCTGTTC CCCTACAGTAACTACAAGCTGGAGATGGTGGTGGTCAATGGGAGAGGTGACGGGCCTCGAAGTGAAACCA AGGAATTCACCACCCCAGAAGGAGTACCCAGTGCCCCCAGGCGGTTCAGAGTCCGACAGCCCAACCTGGA GACCATCAACCTGGAGTGGGACCACCCAGAGCACCCCAACGGAATCCTGATTGGATACATCCTCAGATAC GTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAACTTCTCTCCCAATCAGACCAAGTTCT CTGTGCAGAGAGCAGACCCAGTGTCGCGTTACCGCTTCTCCCTCAGTGCCAGGACACAGGTGGGCTCTGG AGAAGCAGCCACAGAGGAGTCCCCAGCACCTCCAAATGAAGCTACTCCAACTGCAGCTTACACCAATAAC CAGGCAGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGTGCCATTGCCCTTCTGGTGCTGATCC TTCTCATCGTCTGCTTCATCAAGAGGAGTCGAGGTGGCAAGTACCCAGTGCGGGAAAAGAAGGATGTCCC CTTGGGTCCTGAAGACCCCAAAGAAGAAGATGGCTCATTTGACTACAGTGATGAGGACAACAAGCCCCTG CAGGGCAGCCAGACATCTCTGGATGGCACCATCAAGCAGCAGGAGAGCGATGACAGCCTGGTGGACTATG GCGAAGGCGGCGAGGGCCAGTTCAATGAAGATGGCTCCTTTATTGGCCAGTACACTGTCAAAAAGGACAA GGAGGAAACGGAGGGCAATGAGAGCTCAGAGGCCACATCACCAGTCAATGCCATCTATTCCCTTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221047 representing NM_001160316
Red=Cloning site Green=Tags(s) MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR FHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECI ASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW LDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN TSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDP SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLADQATPTNR LAALPKGRPDRPRDLELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAV LHLSPYVNYQFRVIAVNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGP NLRYIVKWRRRETRETWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAP TEVKIRVLNSTAISLQWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLF PYSNYKLEMVVVNGRGDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRY VPFNGTKLGKQMVENFSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNN QADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPL QGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001160316 |
| ORF Size | 3567 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001160316.1, NP_001153788.1 |
| RefSeq Size | 9406 bp |
| RefSeq ORF | 3570 bp |
| Locus ID | 269116 |
| Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223903 | Nfasc (untagged) - Mouse neurofascin (Nfasc), transcript variant 2, (10ug) |
CNY 12,424.00 |
|
| MR221047 | Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 2 |
CNY 11,880.00 |
|
| MR221047L3 | Lenti ORF clone of Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 2 |
CNY 13,780.00 |
|
| MR221047L4 | Lenti ORF clone of Nfasc (mGFP-tagged) - Mouse neurofascin (Nfasc), transcript variant 2 |
CNY 13,780.00 |
