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Kcna4 (NM_021275) Mouse Tagged ORF Clone
CAT＃: MG220977
- TrueORF®
Kcna4 (tGFP-tagged) - Mouse potassium voltage-gated channel shaker-related subfamily member 4 (Kcna4), (10ug)
ORF Plasmid: DDK
"NM_021275" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Kv1.4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220977 representing NM_021275
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTGGCGATGGTGAGTGCCGAGAGCTCAGGGTGCAACAGCCATATGCCTTATGGTTATGCAGCCC AGGCCAGGGCCCGAGAGCGGGAGAGACTCGCTCACTCCAGGGCAGCTGCAGCTGCTGCTGTCGCAGCTGC CACGGCTGCTGTCGAAGGCACTGGGGGTTCTGGTGGAGGCCCCCACCACCATCATCAGACACGTGGGGCC TACTCCTCCCATGATCCTCAAGGTAGCCGTGGAAGTAGAAGGAGGAGGCGTCAGCGAACTGAGAAGAAGA AACTCCACCACAGGCAGAGCAGTTTTCCTCATTGCTCAGACCTGATGCCCAGTGGCTCTGAAGAGAAGAT CCTGAGGGAGCTAAGCGAGGAAGAGGAAGACGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGGAAGGTTT TACTATAGTGAAGAAGACCATGGGGATGGGTGTTCGTACACAGACCTGCTGCCACAGGATGATGGGGGTG GTGGCGGCTACAGTTCAGTCCGCTATAGTGACTGCTGTGAACGTGTGGTGATAAATGTGTCTGGTCTACG CTTCGAAACCCAAATGAAAACTTTGGCCCAGTTTCCAGAAACTCTGTTGGGAGACCCTGAGAAGAGGACT CAGTACTTCGACCCTTTGCGCAATGAGTATTTTTTTGATCGGAACCGACCCAGCTTTGATGCCATTTTGT ATTATTACCAGTCAGGAGGCCGCCTGAAGAGACCAGTCAATGTCCCCTTTGATATCTTCACCGAGGAGGT GAAGTTCTATCAGTTGGGAGAGGAAGCCCTGCTCAAGTTCCGGGAGGATGAGGGCTTTGTGAGAGAAGAG GAGGACAGGGCTCTGCCAGAAAATGAATTTAAAAAACAGATTTGGCTTCTCTTTGAATATCCAGAGAGTT CTAGCCCTGCCAGGGGTATAGCCATTGTGTCTGTCCTGGTCATCTTAATCTCTATTGTCATATTTTGCCT GGAAACCTTGCCGGAGTTCAGGGATGATAGGGACCTTATTATGGCCCTCAGTGCAGGCGGGCACAGCAGA TTGCTGAATGACACCTCGGCACCCCACCTGGAGAACTCAGGGCACACAATATTCAATGACCCTTTCTTCA TCGTGGAGACAGTGTGTATTGTGTGGTTTTCCTTTGAGTTTGTGGTTCGATGCTTTGCTTGTCCCAGCCA AGCACTCTTCTTCAAAAACATCATGAACATCATTGATATCGTCTCCATTTTGCCTTACTTCATCACTCTG GGCACTGACCTGGCCCAACAGCAGGGGGGTGGCAATGGCCAGCAGCAGCAGGCCATGTCCTTTGCCATCC TTAGGATCATTCGTCTGGTCCGAGTATTCCGGATCTTCAAGCTCTCCAGACACTCCAAAGGCCTGCAGAT CCTGGGCCACACCCTAAGAGCCAGCATGCGGGAACTGGGCCTTCTTATCTTTTTCCTCTTCATCGGGGTT ATCCTCTTTTCCAGCGCTGTGTATTTTGCAGAGGCGGATGAACCCACTACCCATTTCCAAAGCATTCCAG ATGCGTTTTGGTGGGCTGTGGTAACCATGACAACTGTGGGCTATGGGGACATGAAGCCCATCACAGTCGG GGGAAAGATTGTGGGGTCCCTGTGTGCCATTGCGGGTGTCTTAACCATTGCTTTGCCTGTGCCGGTGATT GTGTCTAACTTTAACTATTTCTACCACAGAGAGACTGAAAATGAAGAACAGACCCAGCTGACACAAAACG CAGTCAGTTGTCCATACCTACCTTCTAATTTGCTCAAGAAATTTCGGAGCTCCACTTCTTCTTCCCTGGG GGACAAGTCAGAGTATCTAGAGATGGAAGAAGGGGTCAAGGAATCATTATGTGGAAAGGAGGAGAAGTGT CAGGGAAAGGGAGATGAGAGCGAGACAGATAAAAACAACTGTTCTAATGCAAAGGCTGTGGAGACTGATG TG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220977 representing NM_021275
Red=Cloning site Green=Tags(s) MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGTGGSGGGPHHHHQTRGA YSSHDPQGSRGSRRRRRQRTEKKKLHHRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRF YYSEEDHGDGCSYTDLLPQDDGGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRT QYFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREE EDRALPENEFKKQIWLLFEYPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLIMALSAGGHSR LLNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITL GTDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGV ILFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVI VSNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCGKEEKC QGKGDESETDKNNCSNAKAVETDV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021275 |
| ORF Size | 1962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021275.4, NP_067250.2 |
| RefSeq Size | 4844 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 16492 |
| UniProt ID | Q61423 |
| Gene Summary | Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:8020965). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (By similarity). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Homotetrameric KCNA4 forms a potassium channel that opens in response to membrane depolarization, followed by rapid spontaneous channel closure (PubMed:8020965). Likewise, a heterotetrameric channel formed by KCNA1 and KCNA4 shows rapid inactivation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220156 | Kcna4 (untagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 4 (Kcna4), (10ug) |
CNY 8,460.00 |
|
| MR220977 | Kcna4 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 4 (Kcna4) |
CNY 4,872.00 |
|
| MR220977L3 | Lenti ORF clone of Kcna4 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 4 (Kcna4) |
CNY 6,460.00 |
|
| MR220977L4 | Lenti ORF clone of Kcna4 (mGFP-tagged) - Mouse potassium voltage-gated channel, shaker-related subfamily, member 4 (Kcna4) |
CNY 6,460.00 |
