Il1r2 (NM_010555) Mouse Tagged ORF Clone

CAT#: MG220951

  • TrueORF®

Il1r2 (tGFP-tagged) - Mouse interleukin 1 receptor type II (Il1r2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_010555" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms CD121b; Il1r-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG220951 representing NM_010555
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCATCTTGCTTGTGTTAGTAACTGGAGTTTCTGCTTTCACCACTCCAACAGTGGTGCACACAGGAA
AGGTTTCTGAATCCCCCATTACATCGGAGAAGCCCACAGTCCATGGAGACAACTGTCAGTTTCGTGGCAG
AGAGTTCAAATCTGAATTGAGGCTGGAAGGTGAACCTGTGGTTCTGAGGTGCCCCTTGGCACCTCACTCC
GACATCTCCAGCAGTTCCCATAGTTTTCTGACCTGGAGTAAATTGGACTCTTCTCAGCTGATCCCAAGAG
ATGAGCCAAGGATGTGGGTGAAGGGTAACATACTCTGGATTCTGCCAGCAGTGCAGCAAGACTCTGGTAC
CTACATTTGCACATTCAGAAACGCATCCCACTGTGAGCAAATGTCTGTGGAACTCAAGGTCTTTAAGAAT
ACTGAAGCATCTCTGCCTCATGTCTCCTACTTGCAAATCTCAGCTCTCTCCACCACCGGGTTACTAGTGT
GCCCTGACCTGAAAGAATTCATCTCCAGCAACGCTGATGGAAAGATACAGTGGTATAAGGGCGCCATACT
CTTGGATAAAGGCAATAAGGAATTTCTGAGTGCAGGAGACCCCACACGCCTATTGATATCCAACACGTCC
ATGGACGATGCAGGCTATTACAGATGTGTTATGACATTTACCTACAATGGCCAGGAATACAACATCACTA
GGAATATTGAACTCCGGGTCAAAGGAACAACCACGGAACCCATCCCTGTGATCATTTCTCCCCTGGAGAC
AATACCAGCATCATTGGGGTCAAGACTGATAGTCCCGTGCAAAGTGTTTCTGGGAACTGGTACATCTTCC
AACACCATTGTGTGGTGGTTGGCTAACAGCACGTTTATCTCGGCTGCTTACCCAAGAGGCCGTGTGACCG
AGGGGCTACACCACCAGTACTCAGAGAATGATGAAAACTATGTGGAAGTGTCGCTGATTTTTGATCCAGT
CACAAGGGAGGATCTGCATACAGATTTTAAATGTGTTGCCTCGAATCCACGGAGTTCTCAGTCACTCCAT
ACCACAGTCAAAGAAGTCTCTTCCACGTTCTCCTGGAGCATTGCGCTGGCACCTCTGTCTCTGATCATCT
TGGTTGTGGGGGCAATATGGATGCGCAGACGGTGTAAACGCAGGGCTGGAAAGACATATGGACTGACCAA
GCTACGGACTGACAACCAGGACTTCCCTTCCAGCCCAAAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG220951 representing NM_010555
Red=Cloning site Green=Tags(s)

MFILLVLVTGVSAFTTPTVVHTGKVSESPITSEKPTVHGDNCQFRGREFKSELRLEGEPVVLRCPLAPHS
DISSSSHSFLTWSKLDSSQLIPRDEPRMWVKGNILWILPAVQQDSGTYICTFRNASHCEQMSVELKVFKN
TEASLPHVSYLQISALSTTGLLVCPDLKEFISSNADGKIQWYKGAILLDKGNKEFLSAGDPTRLLISNTS
MDDAGYYRCVMTFTYNGQEYNITRNIELRVKGTTTEPIPVIISPLETIPASLGSRLIVPCKVFLGTGTSS
NTIVWWLANSTFISAAYPRGRVTEGLHHQYSENDENYVEVSLIFDPVTREDLHTDFKCVASNPRSSQSLH
TTVKEVSSTFSWSIALAPLSLIILVVGAIWMRRRCKRRAGKTYGLTKLRTDNQDFPSSPN

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010555
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010555.5
RefSeq Size 1360 bp
RefSeq ORF 1233 bp
Locus ID 16178
UniProt ID P27931
Gene Summary Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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