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Nfasc (NM_001160317) Mouse Tagged ORF Clone
CAT＃: MG220935
- TrueORF®
Nfasc (tGFP-tagged) - Mouse neurofascin (Nfasc) transcript variant 3, (10ug)
ORF Plasmid: DDK
"NM_001160317" in other vectors (4)
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CNY 12,830.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA387016; D430023G06Rik; mKIAA0756; NF |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220935 representing NM_001160317
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGCAGGCGCCACCCTGGGTCCACATAGCCCTCATCCTCTTCCTCCTCAGCCTCGGAGGGG CCATCGAGATTCCGATGGACCTGACCCAACCCCCAACTATCACCAAGCAGTCCGTGAAGGACCACATCGT GGACCCTCGAGATAACATCCTGATTGAATGTGAAGCTAAAGGCAACCCCGCCCCCAGTTTTCACTGGACT CGCAACAGCAGATTCTTCAACATTGCCAAGGACCCACGGGTGTCCATGAGGAGGAGATCTGGGACCTTGG TGATCGACTTCCGCAGTGGTGGGCGGCCTGAGGAATACGAAGGGGAGTACCAGTGCTTTGCCCGGAACAA ATTTGGCACTGCACTTAGCAACCGCATCCGCCTGCAGGTGTCCAAATCTCCCCTGTGGCCCAAGGAAAAC CTAGACCCCGTCGTGGTTCAAGAGGGTGCCCCCTTGACACTACAGTGCAACCCCCCACCCGGCCTCCCGT CCCCCGTCATCTTCTGGATGAGCAGCTCCATGGAGCCCATCACCCAGGACAAGCGTGTCTCCCAGGGTCA CAACGGGGACCTGTACTTCTCCAACGTCATGCTGCAGGACATGCAGACCGACTACAGCTGCAACGCGCGC TTTCACTTCACCCACACCATTCAGCAGAAGAACCCCTTCACCCTCAAGGTCCTCACCAACAACCCCTATA ATGACTCGTCCTTAAGAAACCACCCTGACATATATAGTGCCCGAGGAGTTGCAGAAAGAACGCCCAGCTT CATGTATCCCCAGGGCACATCGAGCAGTCAGATGGTTCTCCGTGGCATGGACCTGCTGCTGGAATGCATT GCCTCTGGCGTCCCAACACCAGACATTGCATGGTACAAGAAAGGTGGGGACCTCCCATCTAACAAGGCCA AGTTCGAGAACTTTAATAAGGCTCTGCGCATCACCAATGTCTCTGAAGAGGACTCTGGGGAGTATTTCTG CCTGGCCTCCAACAAGATGGGCAGCATCCGGCACACGATCTCGGTGAGAGTAAAGGCTGCTCCATACTGG CTGGATGAGCCCAAGAACCTGATCCTGGCTCCTGGGGAAGATGGGAGGCTGGTATGCCGAGCCAATGGGA ACCCGAAGCCGACCGTGCAGTGGATGGTGAATGGAGAGCCTTTACAATCGGCACCACCCAATCCCAACCG TGAGGTAGCTGGAGACACTATCATCTTCCGGGATACTCAGATCAGCAGCAGGGCAGTGTACCAATGTAAT ACATCCAATGAACATGGCTACCTGCTGGCCAATGCCTTCGTCAGCGTGTTAGATGTACCCCCTCGGATGC TGTCTGCCCGCAACCAGCTCATCAGGGTGATCCTTTATAACCGGACACGGCTGGACTGTCCGTTCTTTGG GTCTCCCATCCCAACACTCCGATGGTTTAAGAATGGGCAAGGAAGCAACCTGGATGGCGGTAACTACCAC GTCTACGAAAACGGCAGTCTAGAAATCAAGATGATTCGCAAAGAGGACCAAGGCATCTACACCTGTGTGG CCACCAACATCCTGGGCAAAGCCGAAAATCAAGTCCGCCTGGAGGTCAAAGACCCCACCAGGATCTACAG GATGCCCGAGGACCAGGTGGCCAAGAGGGGCACCACGGTGCAGCTGGAGTGCCGCGTGAAACATGACCCC TCCTTGAAGCTCACAGTCTCCTGGCTGAAGGACGATGAGCCACTCTACATTGGAAACAGGATGAAGAAGG AAGATGACTCCCTGACGATCTTCGGAGTGGCAGAGCGGGACCAGGGCAGTTACACGTGTATGGCCAGCAC CGAGCTGGACCAGGACCTGGCAAAGGCCTACCTCACTGTTCTAGGGCGACCAGACCGACCCAGGGACCTG GAGCTCACTGACCTGGCTGAGAGGAGTGTGAGGCTGACCTGGATCCCAGGGGATGACAACAACAGCCCTA TCACAGACTACGTCGTTCAGTTTGAAGAGGACCAGTTCCAACCAGGGGTGTGGCATGACCACTCCAGGTT CCCAGGCAGCGTCAACTCAGCCGTCCTCCATCTGTCCCCATATGTCAACTACCAATTCAGAGTCATCGCT GTCAACGAGGTTGGGAGCAGCCACCCCAGCCTTCCATCCGAGCGGTACCGAACCAGTGGGGCACCCCCTG AATCTAATCCCAGTGATGTGAAGGGCGAAGGGACAAGAAAGAACAATATGGAGATCACGTGGACGCCTAT GAATGCTACCTCTGCCTTTGGCCCCAACCTACGCTACATTGTCAAGTGGCGACGGAGAGAGACCCGAGAG ACTTGGAACAATGTCACAGTGTGGGGCTCTCGCTACGTGGTGGGGCAGACGCCTGTCTACGTTCCCTATG AGATCCGAGTCCAGGCTGAAAATGACTTTGGGAAAGGCCCCGAGCCTGACACCATCATTGGGTACTCCGG AGAAGATTATCCCAGGGCTGCGCCCACTGAAGTTAAAATCCGAGTCCTGAACAGCACAGCCATCAGCCTT CAGTGGAACCGAGTCTACTCTGACACGGTCCAGGGCCAGCTCAGAGAGTATCGAGCTTACTACTGGAGGG AAAGCAGTTTGCTGAAGAACCTGTGGGTGTCTCAGAAGAGACAGCAGGCCAGCTTCCCTGGTGACCGTCC CCGGGGCGTGGTGGCCCGCCTGTTCCCCTACAGTAACTACAAGCTGGAGATGGTGGTGGTCAATGGGAGA GGTGACGGGCCTCGAAGTGAAACCAAGGAATTCACCACCCCAGAAGGAGTACCCAGTGCCCCCAGGCGGT TCAGAGTCCGACAGCCCAACCTGGAGACCATCAACCTGGAGTGGGACCACCCAGAGCACCCCAACGGAAT CCTGATTGGATACATCCTCAGATACGTGCCCTTTAATGGAACCAAACTGGGAAAGCAGATGGTGGAAAAC TTCTCTCCCAATCAGACCAAGTTCTCTGTGCAGAGAGCAGACCCAGTGTCGCGTTACCGCTTCTCCCTCA GTGCCAGGACACAGGTGGGCTCTGGAGAAGCAGCCACAGAGGAGTCCCCAGCACCTCCAAATGAAGCTAC TCCAACTGCAGCTTACACCAATAACCAGGCAGACATCGCCACCCAGGGCTGGTTCATCGGGCTCATGTGT GCCATTGCCCTTCTGGTGCTGATCCTTCTCATCGTCTGCTTCATCAAGAGGAGTCGAGGTGGCAAGTACC CAGTGCGGGAAAAGAAGGATGTCCCCTTGGGTCCTGAAGACCCCAAAGAAGAAGATGGCTCATTTGACTA CAGTGATGAGGACAACAAGCCCCTGCAGGGCAGCCAGACATCTCTGGATGGCACCATCAAGCAGCAGGAG AGCGATGACAGCCTGGTGGACTATGGCGAAGGCGGCGAGGGCCAGTTCAATGAAGATGGCTCCTTTATTG GCCAGTACACTGTCAAAAAGGACAAGGAGGAAACGGAGGGCAATGAGAGCTCAGAGGCCACATCACCAGT CAATGCCATCTATTCCCTTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220935 representing NM_001160317
Red=Cloning site Green=Tags(s) MARQQAPPWVHIALILFLLSLGGAIEIPMDLTQPPTITKQSVKDHIVDPRDNILIECEAKGNPAPSFHWT RNSRFFNIAKDPRVSMRRRSGTLVIDFRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKEN LDPVVVQEGAPLTLQCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNAR FHFTHTIQQKNPFTLKVLTNNPYNDSSLRNHPDIYSARGVAERTPSFMYPQGTSSSQMVLRGMDLLLECI ASGVPTPDIAWYKKGGDLPSNKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVKAAPYW LDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDTIIFRDTQISSRAVYQCN TSNEHGYLLANAFVSVLDVPPRMLSARNQLIRVILYNRTRLDCPFFGSPIPTLRWFKNGQGSNLDGGNYH VYENGSLEIKMIRKEDQGIYTCVATNILGKAENQVRLEVKDPTRIYRMPEDQVAKRGTTVQLECRVKHDP SLKLTVSWLKDDEPLYIGNRMKKEDDSLTIFGVAERDQGSYTCMASTELDQDLAKAYLTVLGRPDRPRDL ELTDLAERSVRLTWIPGDDNNSPITDYVVQFEEDQFQPGVWHDHSRFPGSVNSAVLHLSPYVNYQFRVIA VNEVGSSHPSLPSERYRTSGAPPESNPSDVKGEGTRKNNMEITWTPMNATSAFGPNLRYIVKWRRRETRE TWNNVTVWGSRYVVGQTPVYVPYEIRVQAENDFGKGPEPDTIIGYSGEDYPRAAPTEVKIRVLNSTAISL QWNRVYSDTVQGQLREYRAYYWRESSLLKNLWVSQKRQQASFPGDRPRGVVARLFPYSNYKLEMVVVNGR GDGPRSETKEFTTPEGVPSAPRRFRVRQPNLETINLEWDHPEHPNGILIGYILRYVPFNGTKLGKQMVEN FSPNQTKFSVQRADPVSRYRFSLSARTQVGSGEAATEESPAPPNEATPTAAYTNNQADIATQGWFIGLMC AIALLVLILLIVCFIKRSRGGKYPVREKKDVPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQE SDDSLVDYGEGGEGQFNEDGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001160317 |
| ORF Size | 3522 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001160317.1, NP_001153789.1 |
| RefSeq Size | 9597 bp |
| RefSeq ORF | 3525 bp |
| Locus ID | 269116 |
| Gene Summary | This gene encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. This gene undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined. [provided by RefSeq, May 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223840 | Nfasc (untagged) - Mouse neurofascin (Nfasc), transcript variant 3, (10ug) |
CNY 15,110.00 |
|
| MR220935 | Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 3 |
CNY 11,690.00 |
|
| MR220935L3 | Lenti ORF clone of Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 3 |
CNY 13,590.00 |
|
| MR220935L4 | Lenti ORF clone of Nfasc (mGFP-tagged) - Mouse neurofascin (Nfasc), transcript variant 3 |
CNY 13,590.00 |
