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Ap3b2 (NM_021492) Mouse Tagged ORF Clone
CAT＃: MG220472
- TrueORF®
Ap3b2 (tGFP-tagged) - Mouse adaptor-related protein complex 3 beta 2 subunit (Ap3b2), (10ug)
ORF Plasmid: DDK
"NM_021492" in other vectors (4)
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CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI549966; AU042881; beta-NAP; beta3B; Naptb; [b]-NAP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220472 representing NM_021492
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGCCGCTCCGGCCTACAGCGAAGACAAGGGCGGCTCAGCCGGTCCCGGGGAGCCAGAATATGGCC ACGACCCGGCGAGCGGCGGTATCTTCTCCTCGGACTACAAGCGGCATGATGACCTGAAAGAGATGTTGGA CACCAACAAGGACTCCCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCAATGATCGCCCGGGGAAAGAAT GCCTCGGACCTGTTCCCTGCAGTGGTGAAGAATGTGGCCTGTAAGAACATAGAGGTGAAGAAGCTTGTCT ACGTGTATCTGGTCCGATATGCCGAGGAGCAGCAAGACCTCGCTCTACTGTCCATCTCTACTTTCCAACG TGGCCTAAAGGATCCCAACCAGCTGATCCGTGCCAGTGCGCTCCGGGTTCTCTCTAGCATCCGTGTGCCC ATCATAGTGCCCATCATGATGCTAGCCATCAAGGAAGCTGCCTCGGACATGTCACCCTACGTTCGGAAAA CAGCTGCTCATGCCATCCCCAAACTCTACAGTTTGGACTCCGATCAGAAGGACCAGCTGATAGAAGTCAT TGAGAAGCTTTTGGCAGATAAGACCACGCTGGTGGCAGGCAGTGTAGTGATGGCCTTTGAAGAGGTATGT CCAGAACGTATTGACCTGATTCACAAAAACTACCGGAAGCTCTGCAACCTGCTCATTGATGTGGAGGAGT GGGGCCAGGTGGTGATTATCAGCATGCTCACGCGTTATGCGCGAACGCAATTCCTGAGCCCTACGCAGAA TGAGTCCTTGCTGGAGGAGAATCCCGAGAAGGCCTTCTATGGCTCCGAGGAGGACGAGGCCAAAGGCCCT GGATCAGAGGAGGCAGCCACTGCGGCGCTGCCCGCTCGGAAGCCCTATGTAATGGACCCAGATCACCGGC TGCTGCTGCGGAACACCAAGCCCCTGCTGCAGAGCCGCAGCGCCGCGGTGGTCATGGCGGTGGCGCAGCT TTACTTCCACCTAGCGCCTAAGGCAGAGGTGGGCGTCATCGCCAAGGCGCTGGTACGCCTGCTGCGCAGC CACAGTGAGGTGCAGTACGTAGTGCTCCAGAACGTGGCCACCATGTCCATCAAGCGCAGGGGTATGTTTG AACCCTACCTGAAGAGCTTCTACATTAGGTCCACGGATCCTACCCAGATCAAGATCCTGAAGCTGGAAGT GCTGACCAACCTGGCCAATGAGACTAACATCCCCACTGTTCTCCGGGAATTCCAGACCTACATTCGCAGC ATGGACAAAGACTTTGTGGCAGCCACGATCCAGGCCATTGGACGATGTGCAACGAACATAGGCCGAGTCC GTGACACCTGTCTCAATGGCTTGGTGCAGCTTCTGTCCAACCGTGACGAGCTTGTGGTTGCAGAGTCAGT GGTGGTCATTAAGAAGCTGCTGCAAATGCAGCCAGCGCAGCACGGTGAGATCATCAAACACTTGGCAAAG CTCACAGACAACATCCAGGTGCCCATGGCACGAGCCAGCATCCTCTGGCTCATTGGAGAGTACTGTGAGC ATGTCCCCAAGATCGCACCTGATGTCCTGAGAAAAATGGCCAAGTCCTTCACAGCAGAGGAGGACATCGT GAAGCTGCAGGTCATCAACCTGGCAGCCAAGCTGTACCTGACTAACTCAAAGCAGACCAAGCTCTTGACC CAGTATGTGCTGAGTCTGGCCAAGTACGACCAGAACTATGATATCCGGGATCGAGCCCGCTTCACCCGGC AGCTCATTGTTCCCTCTGAGCAGGGTGGTGCCCTCAGTCGCCATGCCAAGAAACTCTTCCTGGCCCCTAA GCCAGCCCCTATCTTGGAGTCATCCTTCAAAGATCGTGACCATTTCCAGCTGGGCTCACTGTCACACCTG CTCAATGCAAAGGCTACAGGCTACCAGGAGCTCCCGGACTGGCCAGAGGAAGCCCCAGATCCATCTGTGC GCAATGTGGAGGTACCTGAATGGACCAAATGTTCAAATCGGGAGAAGAGGAAGGAGAAGGAAAAGCCTTT CTATTCTGACTCCGAGGGGGAGTCTGGCCCCACTGAGTCTGCAGACAGCGAGCCTGAATCTGAGAGTGAG TCAGAGAGTAAGAGCAGCAGTGGGAGTGGCTCCGGGGAGTCTAGCAGTGAGTCGGACAATGAAGAAGAGG ATGAGGAGAAAGGCGGAGGCAGCGAGAGTGAACAGAGTGAGGAGGAAGATGAGAAGAAGAAGAAGACAAA GAAGAAGAAGGCATCAGAGGGACACAGAGAAGGCTCATCATCAGAAGAGGGCAGTGACTCCAGCAGCAGC TCAGAGTCTGAGGTGACGTCAGAGTCGGAGGAGGAACAGGTGGAACCTGCCTCCTGGAGGAAGAAAACGC CTCCAGGCAGCAAGAGTGCCCCTGTAGCCAAGGAGATTTCCCTGCTGGACCTAGAGGATTTCACCCCTCC TAGTGTCCAGCCTGTGTCTCCTCCCATGGTTGTGTCTACCAGTCTGGCTGCTGACCTGGAAGGGCTGACA CTCACAGACTCCTCTCTGGTGCCTTCGCTGCTGAGTCCAGTGTCCAGCATTGGGAGGCAGGAGCTGCTGC ACCGGGTAGCTGGAGAGGGGCTGTCTGTGGACTATGCCTTCAGCCGCCAGCCTTTCTCTGGGGATCCCCA CATGGTGTCTCTGCATATCTACTTCTCCAACAACTCGGAGACTCCCATCAAGGGGCTGCATGTGGGCACT CCCAAGCTGCCTGCTGGCATCAGCATTCAAGAATTCCCTGAAATCGAGTCCCTGGCACCTGGAGAATCCA CCACTACTGTGATGGGCATTAACTTCTGCGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAAAC CCGACAGTTCTATGTCTCCATTCAGCCACCTGTCGGGGAGCTGATGGCCCCTGTATTTATGAGTGAGAAT GAGTTCAAGAAGGAACAGGGAAAGCTGACAGGCATGAATGAGATCACAGAGAAGCTTACATTGCCAGACA CCTGTCGAAGTGATCACATGGTGGTGCAGAAAGTGACTGCCACCGCCAACCTAGGTCGTGTCCCATGTGG GACATCTGATGAGTACAGGTTTGCAGGGAGGACATTAACTAGTGGGAGTCTGGTCCTGCTGACTCTGGAT GCCCGGGCAGCTGGAGCCGCCCAGCTGACCGTCAACAGTGAGAAGATGGTGATTGGCACCATGCTGGTGA AGGATGTGATTCAGGCTCTGACCCAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220472 representing NM_021492
Red=Cloning site Green=Tags(s) MSAAPAYSEDKGGSAGPGEPEYGHDPASGGIFSSDYKRHDDLKEMLDTNKDSLKLEAMKRIVAMIARGKN ASDLFPAVVKNVACKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRGLKDPNQLIRASALRVLSSIRVP IIVPIMMLAIKEAASDMSPYVRKTAAHAIPKLYSLDSDQKDQLIEVIEKLLADKTTLVAGSVVMAFEEVC PERIDLIHKNYRKLCNLLIDVEEWGQVVIISMLTRYARTQFLSPTQNESLLEENPEKAFYGSEEDEAKGP GSEEAATAALPARKPYVMDPDHRLLLRNTKPLLQSRSAAVVMAVAQLYFHLAPKAEVGVIAKALVRLLRS HSEVQYVVLQNVATMSIKRRGMFEPYLKSFYIRSTDPTQIKILKLEVLTNLANETNIPTVLREFQTYIRS MDKDFVAATIQAIGRCATNIGRVRDTCLNGLVQLLSNRDELVVAESVVVIKKLLQMQPAQHGEIIKHLAK LTDNIQVPMARASILWLIGEYCEHVPKIAPDVLRKMAKSFTAEEDIVKLQVINLAAKLYLTNSKQTKLLT QYVLSLAKYDQNYDIRDRARFTRQLIVPSEQGGALSRHAKKLFLAPKPAPILESSFKDRDHFQLGSLSHL LNAKATGYQELPDWPEEAPDPSVRNVEVPEWTKCSNREKRKEKEKPFYSDSEGESGPTESADSEPESESE SESKSSSGSGSGESSSESDNEEEDEEKGGGSESEQSEEEDEKKKKTKKKKASEGHREGSSSEEGSDSSSS SESEVTSESEEEQVEPASWRKKTPPGSKSAPVAKEISLLDLEDFTPPSVQPVSPPMVVSTSLAADLEGLT LTDSSLVPSLLSPVSSIGRQELLHRVAGEGLSVDYAFSRQPFSGDPHMVSLHIYFSNNSETPIKGLHVGT PKLPAGISIQEFPEIESLAPGESTTTVMGINFCDSTQAANFQLCTQTRQFYVSIQPPVGELMAPVFMSEN EFKKEQGKLTGMNEITEKLTLPDTCRSDHMVVQKVTATANLGRVPCGTSDEYRFAGRTLTSGSLVLLTLD ARAAGAAQLTVNSEKMVIGTMLVKDVIQALTQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021492 |
| ORF Size | 3246 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021492.3, NP_067467.2 |
| RefSeq Size | 3816 bp |
| RefSeq ORF | 3249 bp |
| Locus ID | 11775 |
| UniProt ID | Q9JME5 |
| Gene Summary | Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223471 | Ap3b2 (untagged) - Mouse adaptor-related protein complex 3, beta 2 subunit (Ap3b2), (10ug) |
CNY 13,870.00 |
|
| MR220472 | Ap3b2 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, beta 2 subunit (Ap3b2) |
CNY 7,544.00 |
|
| MR220472L3 | Lenti ORF clone of Ap3b2 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 3, beta 2 subunit (Ap3b2) |
CNY 11,400.00 |
|
| MR220472L4 | Lenti ORF clone of Ap3b2 (mGFP-tagged) - Mouse adaptor-related protein complex 3, beta 2 subunit (Ap3b2) |
CNY 11,400.00 |
