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Grb14 (NM_016719) Mouse Tagged ORF Clone
CAT＃: MG220368
- TrueORF®
Grb14 (tGFP-tagged) - Mouse growth factor receptor bound protein 14 (Grb14), (10ug)
ORF Plasmid: DDK
"NM_016719" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI505286 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220368 representing NM_016719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCACGTCCCTGCAAGACGGGCAGAGCGCCGCGGGCCGGGCAGGCGCCCAGGATTCGCCGCTGGCAG TGCAGGTGTGCCGCGTTGCCCAGGGCAAGGGAGACGCCCAGGACCCGGCGCAGGTCCCCGGACTGCACGC GCTGTCCCCCGCCTCCGATGCGACCCTCCGCGGTGCCATAGACAGGAGAAAAATGAAAGATCTGGATGTT CTGGAAAAGCCACCCATTCCCAACCCCTTTCCTGAGCTCTGCTGCTCTCCGCTTACATCTGTGCTGTCAG CAGGCCTGTTTCCCAGGGCCAATTCAAGGAAGAAGCAGGTGATTAAAGTTTACAGCGAGGATGAAACCAG CAGAGCATTAGAGGTGCCCAGTGACATCACAGCCCGAGATGTTTGCCAGCTGTTGATCCTGAAGAACCAC TATGTGGACGACAACAGCTGGACCCTTTTTGAGCACCTATCTCACATAGGTTTAGAAAGAACCGTAGAGG ACCACGAGCTGCCAACTGAAGTGCTGTCTCACTGGGGAGTGGAAGAAGACAATAAGCTGTATCTTAGAAA GAATTATGCCAAATATGAATTTTTTAAGAACCCAATGTATTTCTTTCCAGAGCACATGGTGTCTTTTGCA GCTGAAATGAATGGTGACAGATCCCCTACACAGATACTGCAGGTGTTTTTAAGCTCCAGCACGTATCCTG AAATCCATGGCTTCTTACATGCAAAGGAACAGGGAAAGAAGTCTTGGAAAAAAGCTTACTTTTTTCTCAG AAGATCTGGCTTATATTTTTCTACTAAAGGCACATCCAAGGAACCACGGCATTTGCAGCTTTTCAGTGAA TTCAGCACTAGTCACGTTTATATGTCACTGGCAGGAAAAAAAAAACACGGAGCGCCAACTCCCTATGGAT TCTGCTTAAAGCCTAACAAAGCAGGAGGGCCCCGGGACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAG CAGGACGTGCTGGGTGACCGCCATCCGACTGCTGAAGGATGGCATGCAGCTGTATCAGAATTATATGCAT CCATACCAAGGTAGAAGCGCCTGCAATTCTCAGAGCATGTCACCCATGAGAAGCGTATCAGAGAATTCCC TAGTAGCAATGGACTTCTCAGGTGAGAAGAGCAGAGTCATAGACAACCCCACTGAAGCGCTTTCGGTTGC TGTTGAGGAAGGCCTCGCGTGGAGGAAAAAAGGCTGTTTACGCCTGGGGAATCACGGAAGCCCCAGTGCC CCCTCCCAGAGCTCTGCTGTGAACATGGCTCTCCATCGGTCCCAACCATGGTTTCACCACAGAATTTCCA GAGATGAGGCTCAGCGGCTGATCATTCGGCAGGGGCCTGTGGATGGAGTTTTCTTGGTACGGGATAGTCA GAGTAACCCCAGAACTTTTGTACTGTCAATGAGTCATGGACAAAAGATAAAACACTATCAAATTATACCC GTAGAAGATGATGGTGAGCTGTTCCATACTCTGGATGATGGCCATACGAAGTTCACAGACCTCATCCAGC TGGTGGAGTTCTACCAGCTCAACAGGGGGGTCCTTCCTTGCAAGCTGAAGCATTACTGTGCTAGGATGGC TGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220368 representing NM_016719
Red=Cloning site Green=Tags(s) MTTSLQDGQSAAGRAGAQDSPLAVQVCRVAQGKGDAQDPAQVPGLHALSPASDATLRGAIDRRKMKDLDV LEKPPIPNPFPELCCSPLTSVLSAGLFPRANSRKKQVIKVYSEDETSRALEVPSDITARDVCQLLILKNH YVDDNSWTLFEHLSHIGLERTVEDHELPTEVLSHWGVEEDNKLYLRKNYAKYEFFKNPMYFFPEHMVSFA AEMNGDRSPTQILQVFLSSSTYPEIHGFLHAKEQGKKSWKKAYFFLRRSGLYFSTKGTSKEPRHLQLFSE FSTSHVYMSLAGKKKHGAPTPYGFCLKPNKAGGPRDLKMLCAEEEQSRTCWVTAIRLLKDGMQLYQNYMH PYQGRSACNSQSMSPMRSVSENSLVAMDFSGEKSRVIDNPTEALSVAVEEGLAWRKKGCLRLGNHGSPSA PSQSSAVNMALHRSQPWFHHRISRDEAQRLIIRQGPVDGVFLVRDSQSNPRTFVLSMSHGQKIKHYQIIP VEDDGELFHTLDDGHTKFTDLIQLVEFYQLNRGVLPCKLKHYCARMAV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016719 |
| ORF Size | 1614 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016719.1, NP_057928.1 |
| RefSeq Size | 1978 bp |
| RefSeq ORF | 1617 bp |
| Locus ID | 50915 |
| UniProt ID | Q9JLM9 |
| Gene Summary | Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218300 | Grb14 (untagged) - Mouse growth factor receptor bound protein 14 (Grb14), (10ug) |
CNY 6,940.00 |
|
| MR220368 | Grb14 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 14 (Grb14) |
CNY 4,016.00 |
|
| MR220368L3 | Lenti ORF clone of Grb14 (Myc-DDK-tagged) - Mouse growth factor receptor bound protein 14 (Grb14) |
CNY 6,460.00 |
|
| MR220368L4 | Lenti ORF clone of Grb14 (mGFP-tagged) - Mouse growth factor receptor bound protein 14 (Grb14) |
CNY 6,460.00 |
