Apoc1 (NM_007469) Mouse Tagged ORF Clone
CAT#: MG220182
- TrueORF®
Apoc1 (tGFP-tagged) - Mouse apolipoprotein C-I (Apoc1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_007469" in other vectors (5)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | apo-CI; Apo-CIB; apoC-I; ApoC-IB |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG220182 representing NM_007469
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCTCTTCATCGCTCTTCCTGTCCTGATTGTGGTCGTAGCCATGACCTTGGAAGGCCCAGCCCCCG CCCAGGCGGCCCCGGATTTGTCCGGAACATTGGAGAGCATACCGGATAAACTGAAGGAGTTTGGGAACAC TTTGGAAGACAAGGCCCGGGCAGCCATTGAACATATCAAACAGAAGGAAATTTTGACCAAGACCCGGGCC TGGTTCTCAGAGGCATTTGGCAAAGTGAAGGAGAAGTTGAAGACCACGTTCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220182 representing NM_007469
Red=Cloning site Green=Tags(s) MRLFIALPVLIVVVAMTLEGPAPAQAAPDLSGTLESIPDKLKEFGNTLEDKARAAIEHIKQKEILTKTRA WFSEAFGKVKEKLKTTFS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007469 |
ORF Size | 264 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007469.5 |
RefSeq Size | 515 bp |
RefSeq ORF | 267 bp |
Locus ID | 11812 |
UniProt ID | P34928 |
Gene Summary | This gene encodes a precursor plasma protein that is cleaved to yield a signal peptide and two alternatively processed mature peptides. The encoded protein, which is a component of chylomicrons, very low density lipoproteins and high density lipoproteins, transports lipids from the intestines to other locations in the body. This protein binds to free fatty acids preventing their uptake by cells. This protein is a cofactor for lecithin cholesterol acyltransferase, an enzyme that catalyzes the conversion of free cholesterol to cholesteryl esters. The encoded protein inhibits the activity of the cholesteryl ester transfer protein which promotes the exchange of neutral lipids between lipoproteins. In humans this gene is associated with risk of coronary artery disease and age-associated memory impairment. Mice lacking this gene demonstrate impaired memory. This gene is clustered with three other apolipoprotein genes on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202950 | Apoc1 (untagged) - Mouse apolipoprotein C-I (Apoc1), transcript variant 1, (10ug) |
CNY 1,200.00 |
|
MG200207 | Apoc1 (tGFP-tagged) - Mouse apolipoprotein C-I (Apoc1) |
CNY 2,850.00 |
|
MR220182 | Apoc1 (Myc-DDK-tagged) - Mouse apolipoprotein C-I (Apoc1), transcript variant 1 |
CNY 1,200.00 |
|
MR220182L3 | Lenti ORF clone of Apoc1 (Myc-DDK-tagged) - Mouse apolipoprotein C-I (Apoc1), transcript variant 1 |
CNY 4,750.00 |
|
MR220182L4 | Lenti ORF clone of Apoc1 (mGFP-tagged) - Mouse apolipoprotein C-I (Apoc1), transcript variant 1 |
CNY 4,750.00 |