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Aup1 (NM_007517) Mouse Tagged ORF Clone
CAT＃: MG220166
- TrueORF®
Aup1 (tGFP-tagged) - Mouse ancient ubiquitous protein 1 (Aup1), (10ug)
ORF Plasmid: DDK
"NM_007517" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA589454 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220166 representing NM_007517
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAAAGACTCCGCCTTCCCAGGGGCCCCCGCGGCCCTGCGAAGATGGCGGCGACAGCGGCCCCGCT CTCCTGAAGCGGCTGCGATGGAACCTCCCCCAGCTCCGGGGCCGGAGCGGCTCTTTGACTCGCACCGGCT CCCGAGTGACGGCTTCCTGCTGCTCGCGCTACTGCTCTACGCGCCGGTCGGCCTCTGCCTCCTGGTCCTG CGACTCTTCCTCGGGCTCCACGTCTTCCTGGTCAGCTGCGCTCTGCCCGACAGCGTCCTCCGCAGGTTCG TGGTACGAACCATGTGTGCGGTCCTGGGGCTCGTGGCCCGGCAGGAGGACTCCGGACTCCGCGATCACCG CGTCAGGGTCCTTATTTCCAACCACGTAACACCTTTTGACCACAACATAGTCAACCTCCTCACCACCTGT AGCACCCCTCTACTCAATAGTCCCCCCAGCTTTGTGTGTTGGTCTCGGGGCTTCATGGAGATGGATAGGC GGGTGGAGTTGGTGGAGTCACTCAAGAAATTCTGTGCTTCCACGAGGCTTCCGCCCACACCTTTGCTGCT CTTCCCCGAGGAAGAGGCCACCAATGGCCGAGAAGGGCTGCTGCGTTTCAGTTCGTGGCCATTTTCTATT CAGGACGTGGTACAACCTCTTACCCTGCAAGTTCAGAGACCCCTGGTCTCTGTGACGGTGTCAGATGCCT CCTGGGTCTCAGAACTGCTGTGGTCCCTTTTTGTTCCTTTCACGGTGTATCAAGTAAGGTGGCTTCATCC CATTCGTCGACAGCTAGGGGAAGAGAGTGAGGAGTTTGCGCTCCGTGTACAACAGCTGGTGGCCAAAGAA TTGGGCCAGATAGGGACACGGCTCACTCCAGCAGACAAAGCAGAACACATGAAGCGACAAAGACACCCCA GATTACGCCCCCAGTCAGTGCAGTCTTCTTTTCCTTCTCCTCCCAGCCCTTCTTCTGATGTGCAGCTGAC CACTCTCGCTCACAGAGTCAAGGAGGTTCTGCCCCATGTGCCATTGAATGTCATCCAGAGAGACCTGGCC AGGACTGGGTGTGTAGACTTGACCATCACAAACCTGCTCGAGGGGGCTGTGGCTTTCATGCCTGAAGATG TCACTGAGGGATCTCAGTCCCCGCCTGCACCCTCTGCCCCAAAGTTCCCCAGCTCGGGCCTGGCGACCCC TCAGCCCACAGCCCTAACGTTTGCCAAGTCTTCCTGGGCCCGTCAGGAGAGCCTGCAGGAGCGCAAGCAG GCACTGTATGAATATGCAAGAAGGAGATTCAGAGAGAGACAGGCCCAGGAGGCTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220166 representing NM_007517
Red=Cloning site Green=Tags(s) MPKDSAFPGAPAALRRWRRQRPRSPEAAAMEPPPAPGPERLFDSHRLPSDGFLLLALLLYAPVGLCLLVL RLFLGLHVFLVSCALPDSVLRRFVVRTMCAVLGLVARQEDSGLRDHRVRVLISNHVTPFDHNIVNLLTTC STPLLNSPPSFVCWSRGFMEMDRRVELVESLKKFCASTRLPPTPLLLFPEEEATNGREGLLRFSSWPFSI QDVVQPLTLQVQRPLVSVTVSDASWVSELLWSLFVPFTVYQVRWLHPIRRQLGEESEEFALRVQQLVAKE LGQIGTRLTPADKAEHMKRQRHPRLRPQSVQSSFPSPPSPSSDVQLTTLAHRVKEVLPHVPLNVIQRDLA RTGCVDLTITNLLEGAVAFMPEDVTEGSQSPPAPSAPKFPSSGLATPQPTALTFAKSSWARQESLQERKQ ALYEYARRRFRERQAQEAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007517 |
| ORF Size | 1317 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007517.4, NP_031543.2 |
| RefSeq Size | 1527 bp |
| RefSeq ORF | 1233 bp |
| Locus ID | 11993 |
| Gene Summary | The protein encoded by this gene contains several conserved domains including a hydrophobic domain, an acetyltransferase domain, a ubiquitin binding domain, and a domain required for recruitment of ubiquitin-conjugating enzyme E2 G2 (Ube2g2). In humans, this protein localizes to the endoplasmic reticulum and to lipid droplets. This protein is thought to be involved both in the degradation of misfolded proteins from the endoplasmic reticulum and in the storage of neutral lipids. Reduced expression of the human ortholog of this gene strongly reduces lipid droplet clustering in the cell, and causes stabilization of misfolded proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215776 | Aup1 (untagged) - Mouse ancient ubiquitous protein 1 (Aup1), (10ug) |
CNY 5,700.00 |
|
| MR220166 | Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein 1 (Aup1) |
CNY 3,656.00 |
|
| MR220166L3 | Lenti ORF clone of Aup1 (Myc-DDK-tagged) - Mouse ancient ubiquitous protein 1 (Aup1) |
CNY 5,230.00 |
|
| MR220166L4 | Lenti ORF clone of Aup1 (mGFP-tagged) - Mouse ancient ubiquitous protein 1 (Aup1) |
CNY 5,230.00 |
