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Arhgef28 (NM_012026) Mouse Tagged ORF Clone
CAT＃: MG220108
- TrueORF®
Arhgef28 (tGFP-tagged) - Mouse Rho-guanine nucleotide exchange factor (Rgnef), (10ug)
ORF Plasmid: DDK
"NM_012026" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9230110L08Rik; AI323540; D13Bwg1089e; p190RhoGEF; Rgnef; RhoGEF; Rhoip2; RIP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG220108 representing NM_012026
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTTGAGCTGCAGTGAAGTGCCTCTTTATGGACAGAAGACGGTCTATGCAAAGTTTGGCAAAAACG TGTATCTACCCGAAGATGCGGAGTTTTACTTTGTTTATGGCGGGTCGCATCAGAGGCACGTGGTGATTGC TGACCGTGTCCAGGACAATGTCCTCCAATCTAGCATTCCAGGCCATTGGCTTCAGGAGACAGTGACGGTA TCTGTGTGCCTCTGCTCGGAAGGGTACTCCCCAGTGACCATGGGCTCTGGCTCAGTGACCTACGTGGACA ACATGGCCTGCAGGCTGGCTCGTCTGCTGGTCACTCAGGCTGACCGACTCACAGCCTGCAGTCACCAGAC GCTGCTGACCCCGTTTGCCCTGACAGTGGAAGCGCTGCCTGCCTTGGATGAGGAGCTCGTGCTGGCTCTG ACCCAACTGGAGCTGCCTTTGGGTTGGACAGTCCTGGGAAATTCCTCACTTGAAGTATCTCTACACAGAG AGTCTCTCCTGCACCTGGCTGTGAGATGGGCCCTGCCTAAGCTATTCCACTTCCTCTTGTGTCTCCCTGG AGGAGTCAAGGCCTTGAAGCTACCTAATGAAGAGGCCACCACACCATTAGACTTAGCTTTGCAAGGTGGA CACTCCACACTGGTAGAAGACATCACAAATTTTCAGGGTAGCCATTCCCCAGGCTTCTCCCGACTGCGTC TTAACGAAGAGGCCACTCTGCAGTTTGTTCATTCATCGGAAACATTGACCCTGACCGTTAACCACACAGC TGAGCATCTCTTGGAAGCAGACATCAAACTCTTCCGGAAATATTTCTGGGACAGAGCCTTTCTTGTCAAG GCTCTTGAGCAAGAAGCCAAGACTGAGAAAGCAACCATGCCTTCTGGAGCTGCAGAAACTGAAGAAGAGG TTAGGAATTTGGAGTCTGGCAGATCACCATCGGAGGAGGAGGAGGATGCAAAGAGCATCAAAAGCCAGGT GGATGGGCCGAGTGAACACGAAGACCAGGACCGCCTAGCTCTGGATCGCTCCTTTGATGGCCTCAAAAAA TCCAAGCATGTCCCAGCGTCCCTTGCTGCAGGCCAGCTCTCTGATGTTCTGAACGGGGGTGATGAAGTCT ACGCTAACTGTATGGTGATTGACCAGGTTGGCGATTTGGATATCAACTATATAAACCTAGAGGGACTCTC CACCCATACGAGTCCCGAGTCTGGGAGGTCCATGCTGGGGCCTCAGGCTTGCATGCACACCCTTCCTCCC GACACCAGTCCCTGCGGGCGCCCACTCATTGAGAACAGTGAGGGGACCCTGGATGCTGCAGCTTCGCAGT CTTTCGTGACACCATCCAGTTCTCGCACTTCCAACTTGAACCTTTCTTTTGGTCTCCATGGATTTGAAAA GGAGCAGAGTCATCTCAAGAAAAGGAGTTCTAGCCTGGATGCCCTGGTTGCCGACAGTGAGGGGGAAGGA GGTTCTGAGCCCCCCATCTGCTATGCTGTGGGCTCTCAGAGTTCCCCAAGAACTGGACTCCCCAGCGGGG ATGAACTGGACTCTTTTGAGACCAACACTGAACCAGACTGTAATATCTCCAGGACCGAATCACTCTCTCT GTCCAGTACTCTGCATTCAAAGGAGTCCTTGCTTTCTGGGATCCGGTCTCGCTCCTACTCCTGCTCATCA CCCAAAATCTCTTCAGGAAAATCCCGGCTAGTGCGCGACTTCACAGTGTGCAGTACCAGCGAAGAACAGA GATCATATAGTTTCCAGGAGCCACCAGGAGAAAAAAGGATTCAGGAAGAAGAATGGGATGAATACGTCAT TCCTGCCAAATCCGAGTCTGAAAAGTACAAAGTGAGCCGAACTTTCAGCTTCCTCATGAACAGGATGACC AGCCCACGGAACAAATCTAAGATGAAAAACAAGGACACTAAAGAAAAAGAAAAAATGAATCGACATCAGT TTGTCCCGGGAACGTTCTCCGGGGTTCTGCAGTGCTCCGGTTGTGATAAAACACTCCTGGGGAAAGAGTC ACTCCAGTGTGCCAACTGTAAGGCAAACACACACAAGGGCTGCAAAGATGCTGTGCCTCCGTGTACCAAG AAATTCCAAGAGAAATACAACAAGAACAAACCACAGAGCATCCTTGGAAGCTCTTCAGTCAGAGACGTCC CAGCACCTGGGCTCTCCTTGCACCCTTCTTCCTCTATGCCCATCGGACTGCCAGCTGGAAGGAAGGAATT TGCGGCTCAGGTGCATCCACTGTCCAGAAGTGTCCCCGGCACCACTTTGGAAAGCTTCAGGAGGGCAGTG ACATCGTTAGAGTCTGAGGGTGACAGCTGGAGAAGCAGGTCTCATTCCGATGAGCTGTTCCAGTCTATGG GCTCTTCTCCTTCCACGGAGTCATTCATGATGGAAGATGTTGTGGATTCTTCTCTGTGGATCGACCTCAG TAGTGATGCTCAGGAGTTTGAAGCAGAGTCTTGGAGTCTCGTGGTGGACCCTTCCTTTTGCAGTAGGCAG GAGAAGGATGTCATCAAAAGACAAGATGTCATTTTTGAGCTGATGCAGACAGAGGTCCACCACATCCAGA CTCTGCTCATCATGTCCGAGGTCTTCAGGAAAGGCATGAAGGAGGAACTGCAGCTGGACCACAGCACGGT GGACAAGATCTTCCCCTGCTTAGATGAGCTGCTGGAGACTCACCGGCACTTCTTCTTCAGCATGAAGGAG CGCAGGCAGGAGTCCTGCGCTGGCAGTGACAGGAACTTTGTGATCAACCAAATTGGAGACATTCTGGTAC AACAGTTTTCAGAGGAAAATGCAAGCAAAATGAAGAGGATCTATGGAGAATTCTGCAGCCATCACAAAGA AGCCATGAGTCTCTTTAAAGAGCTGCAGCAGAACAAGAAGTTTCAGAATTTTATTAAGATCCGAAATAGC AACCTGTTGGCTCGTCGCCGCGGAATCCCGGAGTGCATCCTGCTAGTTACCCAGCGCATCACAAAGTACC CAGTCTTGGTGGAAAGGATCCTGCAGTACACCAAGGAAAGGACTGAAGAGCACCGGGACCTGTGCAAAGC TCTCGGCTTAATTAAAGACATGATCGCAGCCGTGGACCTGAAAGTCAGCGAATACGAGAAGAACCAAAAA TGGCTCGAGATCCTAAATAAAATCGAGAACAAAACATACACAAAGCTCAAAAATGGCCACGTGTTTAGAA AGCAGGCGCTGCTGAGCCAGGAACGGGCTCTCCTGCATGATGGGCTTGTCTACTGGAAAACCGCTACGGG TCGCTTCAAAGACATCCTGGCTCTGCTACTAACTGACGTGCTGCTCTTCTTACAAGAAAAAGACCAGAAA TACATCTTTGCAGCTGTTGACCAGAAGCCATCAGTGATTTCCCTCCAGAAGCTGATTGCTAGAGAAGTTG CCAACGAGGAGAGAGGCATGTTTCTGATCAGTGCTTCGTCAGCCGGCCCCGAGATGTACGAAATCCACAC CAATTCCAAGGAGGAGCGGAATAACTGGATGAGGCGGATCCAGCAGGCAGTAGAAAGTTGTCCAGAGGAA GAAGGAGGAAGGACGAGCGAGTCGGATGAAGAGAGGCGGAAAGCTGAAGCTAGAGTGGCCAAGATTCAGC AATGTCAAGAAATACTCAGTAACCAAGACCAACAGATTTGCACGTACTTGGAAGAAAAGCTGCATATCTA CGCTGAACTTGGGGAACTGAGTGGCTTTGAGGATGTCCACCTAGAACCCCATCTGCTCATCAAACCTGAC CCTGGAGAGCCTCCCCAGGCAGCGTCCCTGCTTGCAGCAGCCCTGAGAGAAGCCGAGAGTCTGCAAGTGG CTGTGAAGGCTTCAAAGATGGGCGATGTAAGCCAGTCATCCGAAGAAAGCCCTGGAGGGACTGTCCTGAT GGACACACCCAGTACACAGGATGTACCAGCGTCACCAACTGCTTCATTAGTCACAGAAGGGACAGAAGGA AGAGGCTGTTGGGATGTGGATCCCGGGCTCCAGGGTGTGGTAACCGACTTGGCAGTCTCTGATGCAGGGG AGAAGGTGGAATATAGAAGTTTCTCCGGTTCTTCACAGTCAGAGATTATACAAGCAATACAGAATTTAAC GCGTCTCCTCTACAGCCTTCAGGCTGCCTTGACCATCCAGGACAGTCACATTGAGATCCACAAACTGGTT CTACAGCAGCGGGAGAGCTTGGCCCCTAGCCACTCCTTCCGAGGTGGCCCCTTGCAAGACCAGGAGAAAT CCCGCTACCTGGAAAAGCAGCGTGAAGAGCTGGCCAACATCCACAAGCTTCAACACCAGTTCCAGCAGGA GCAGCGGCGCTGGCACCGCACGTGCGACCAGCAACAGCGCGAGCAGGAAGCCCAAGAGAGCTGGCTGCAG GCGCGGGAACGTGAGTGCCAGTCCCAGGAGGAATTGTTGCTGCGCCACCGTAGCGAGCTGGACCACCAAC TCCAGGAGTACCAGCAGAGCCTTGAGCGGCTACGGGAGGGCCAGCGGATGGTGGAGAGGGAGAGACAGAA GATGCGTGTCCAGCAGGGTCTGCTGGGCCACTGCAAGCATAGTCGCCAGCGGAGCCTCCCAGCCGTCTTC TCTCCAGGGAGCAAGGAGGTAACAGAACTTAACCGAGCTGAGAGTTTGTGTCATGAAAATTCATTTTTCA TCAATGAAGCTTTTGGACACATGTCACTTAACACGTCCAATAAACCAAACCCATCAGGGGTCCCCTGGGA TGCTCACCCCCTGGAGGGGTCTCATTTTGATTTGGCAAGGACTAGTGAGAGTCCAACAGAACTGAAGATA GACATTTCTCAGCCTCCTGATGTCAACTCTGAACTCTGGACAACGGGGCCGGGCCATCAGAGACCTGCTC TCCAAGAAAACAGCAAGGAGTCTTATAAAAATGTTGCAGATTTGGACTCCTTCCAGAGTGAATCGTCATC TCCCCAGGACTCCAATCAGCGTGGCCCCCAGCCACAGACACTCACAACAGAAGCAAAACTAAGTCTTCCG ATGGCGGCTGGACACGGTGGCGATGCTGGAGACGGGGCTGAAGAAAACATTCTTTACCTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220108 representing NM_012026
Red=Cloning site Green=Tags(s) MELSCSEVPLYGQKTVYAKFGKNVYLPEDAEFYFVYGGSHQRHVVIADRVQDNVLQSSIPGHWLQETVTV SVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQADRLTACSHQTLLTPFALTVEALPALDEELVLAL TQLELPLGWTVLGNSSLEVSLHRESLLHLAVRWALPKLFHFLLCLPGGVKALKLPNEEATTPLDLALQGG HSTLVEDITNFQGSHSPGFSRLRLNEEATLQFVHSSETLTLTVNHTAEHLLEADIKLFRKYFWDRAFLVK ALEQEAKTEKATMPSGAAETEEEVRNLESGRSPSEEEEDAKSIKSQVDGPSEHEDQDRLALDRSFDGLKK SKHVPASLAAGQLSDVLNGGDEVYANCMVIDQVGDLDINYINLEGLSTHTSPESGRSMLGPQACMHTLPP DTSPCGRPLIENSEGTLDAAASQSFVTPSSSRTSNLNLSFGLHGFEKEQSHLKKRSSSLDALVADSEGEG GSEPPICYAVGSQSSPRTGLPSGDELDSFETNTEPDCNISRTESLSLSSTLHSKESLLSGIRSRSYSCSS PKISSGKSRLVRDFTVCSTSEEQRSYSFQEPPGEKRIQEEEWDEYVIPAKSESEKYKVSRTFSFLMNRMT SPRNKSKMKNKDTKEKEKMNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHKGCKDAVPPCTK KFQEKYNKNKPQSILGSSSVRDVPAPGLSLHPSSSMPIGLPAGRKEFAAQVHPLSRSVPGTTLESFRRAV TSLESEGDSWRSRSHSDELFQSMGSSPSTESFMMEDVVDSSLWIDLSSDAQEFEAESWSLVVDPSFCSRQ EKDVIKRQDVIFELMQTEVHHIQTLLIMSEVFRKGMKEELQLDHSTVDKIFPCLDELLETHRHFFFSMKE RRQESCAGSDRNFVINQIGDILVQQFSEENASKMKRIYGEFCSHHKEAMSLFKELQQNKKFQNFIKIRNS NLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHRDLCKALGLIKDMIAAVDLKVSEYEKNQK WLEILNKIENKTYTKLKNGHVFRKQALLSQERALLHDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQK YIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEE EGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGELSGFEDVHLEPHLLIKPD PGEPPQAASLLAAALREAESLQVAVKASKMGDVSQSSEESPGGTVLMDTPSTQDVPASPTASLVTEGTEG RGCWDVDPGLQGVVTDLAVSDAGEKVEYRSFSGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHKLV LQQRESLAPSHSFRGGPLQDQEKSRYLEKQREELANIHKLQHQFQQEQRRWHRTCDQQQREQEAQESWLQ ARERECQSQEELLLRHRSELDHQLQEYQQSLERLREGQRMVERERQKMRVQQGLLGHCKHSRQRSLPAVF SPGSKEVTELNRAESLCHENSFFINEAFGHMSLNTSNKPNPSGVPWDAHPLEGSHFDLARTSESPTELKI DISQPPDVNSELWTTGPGHQRPALQENSKESYKNVADLDSFQSESSSPQDSNQRGPQPQTLTTEAKLSLP MAAGHGGDAGDGAEENILYL SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012026 |
| ORF Size | 5100 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012026.2, NP_036156.2 |
| RefSeq Size | 6259 bp |
| RefSeq ORF | 5103 bp |
| Locus ID | 110596 |
| UniProt ID | P97433 |
| Gene Summary | Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.[UniProtKB/Swiss-Prot Function] |
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