Rufy1 (NM_172557) Mouse Tagged ORF Clone
CAT#: MG219869
- TrueORF®
Rufy1 (tGFP-tagged) - Mouse RUN and FYVE domain containing 1 (Rufy1), (10ug)
ORF Plasmid: DDK
"NM_172557" in other vectors (4)
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CNY 6,370.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 3000002E04Rik; Rabip4; ZFYVE12 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219869 representing NM_172557
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCCGCGGAGAATGCCGCCGGGCGGGGCAGGATTCGGAGCCGGAGCCGGAGCGGGAGCCGGAGT CCGAGCCGGGGCCGGAGCCTGAGCCCCAAGCAGGCCTAGAGTCCGGGGAAGCGTTCGAGATCGTGGACCG GAGCCAGCTGCCGAGTCCCGGCGAGCTGCGGAGTGCGTCGCGGCCTCGAGTGGCCGACAGCTGGTCGGCG CCCATCCTGACGCTGGCGCGCAGAGCCACCGGGAACCTGTCGGCGAGCTGCGGCAGCGCGCTGCGCGCGG CCGCGGGGCTGGGAGACGGCGGCGGCGGCGGGGAGCGCGCAGCTTCCAAAGGCCAGATGATGGAGGAGCG TGCCAACCTAATGCACATGATGAAACTCAGCATTAAGGTCTTACTCCAGTCTGCCCTTAGCCTGGGACGC AGTCTGGATGCAGACTATGCCCCCTTGCAGCAGTTCTTTGTAGTGATGGAGCATTGCCTCAAACATGGGC TGAAAGTCAAGAAGAGTTTCATTGGCCAAAATAAGTCTTTCTTTGGTCCTTTGGAGCTGGTGGAAAAACT TTGTCCTGAAGCATCAGATATAGCTACCAGTGTCAGGAATCTTCCAGAACTAAAGACAGCCGTGGGAAGA GGCAGAGCGTGGCTTTATCTTGCTCTCATGCAAAAGAAACTGGCAGATTATCTGAAAGTCCTCATAGACA ACAAGCAGCTCCTAAGTGAGTTCTATGAGCCCGAGGCCTTAATGATGGAGGAGGAAGGAATGGTGATCGT GGGTCTGCTGGTGGGACTCAACGTTCTGGATGCCAACCTCTGCTTAAAGGGAGAAGACTTGGATTCTCAG GTCGGGGTGATTGACTTCTCTCTCTGTCTGAAGGACGCACAGGATCTTGACAGCGGCAGAGAGCATGAAA GAATTACCGATGTCCTTGATCAGAAAAATTACGTGGAGGAACTTAACCGGCACCTAAGCTGCACAGTTGG GGATCTTCAGACGAAGATAGATGGCTTGGAAAAGACGAACTCAAAGCTTCAAGAAGAGCTTTCAGCTGCA ACAGACCGAATTTGTTCCCTACAAAAAGAACAACAGCAGCTGAGAGAACAGAACGAAGTAATTCGAGAAA GAAGCGAGAAGAGCGTGGAGATAACAAAACAGGACACCAAGGTTGAACTGGAGACTTATAAGCAGACCCG GCAGGGCCTGGATGAAATGTACAGCGATGTGTGGAAACAGCTGAAGGAGGAGAAGAAAGTCCGACTGGAA CTGGAAAAGGAGCTGGAGTTGCAGATTGGAATGAAGACGGAGATGGAGATCGCCATGAAGTTGCTGGAGA AGGACACCCATGAGAAGCAAGACACGCTCGTTGCCCTCCGCCAGCAGCTGGAGGAAGTCAAAGCTATTAA CTTACAGATGTTTCACAAAGTTCAGAGTGCAGAGAGCAGTTTACAGCAGAAGAATGAAGCCATCGCATCT TTTGAAGGAAAAACCACTCAGGTGATGTCCAGCATGAAGCAAATGGAGGAAAGGTTACAGCAGGCGGAAC GGGCCAGGCAGGCGGCAGAAGAGCGAAGCCACAAGTTGCAGCAGGAGCTCAGCGGGAGGGGCAGTGCCCT GCAGCTCCAGCTGTCCCAGCTGCGGGACCAATGCTCAGGTCTAGAGAAAGAACTGAAGTCAGAAAAAGAA CAAAGACAAGCTCTCCAGAGAGAACTACAGCGTGAGAAAGACACCTCTTGTCTGCTCCAGACGGAGCTGC AGCAAGTGGAAGGCCTGAAGAAGGAGCTGCGGGAGCTCCAAGACGAGAAGGCAGAGTTACGGAAGGTCTG TGAGGAGCAGGAGCAAGCCCTCCAGGAAATGGGACTACACCTTAGCCAGTCCAAACTGAAGATGGAAGAC ATAAAGGAAGTAAATAAGGCACTGAAGGGCCACACATGGTTGAAAGATGACGAGGCAACACATTGTAAGC AGTGTGAGAAGGACTTCTCCATTTCTCGGAGGAAGCACCACTGCCGAAACTGTGGCCACATTTTCTGCAA CACCTGCTCTAGCAACGAGCTGGCCCTGCCCTCCTACCCTAAGCCAGTGCGTGTGTGTGACAGCTGCCAT ACCCTGCTACTGCAGCGCTGCTCCTCCACTGCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219869 representing NM_172557
Red=Cloning site Green=Tags(s) MAARGECRRAGQDSEPEPEREPESEPGPEPEPQAGLESGEAFEIVDRSQLPSPGELRSASRPRVADSWSA PILTLARRATGNLSASCGSALRAAAGLGDGGGGGERAASKGQMMEERANLMHMMKLSIKVLLQSALSLGR SLDADYAPLQQFFVVMEHCLKHGLKVKKSFIGQNKSFFGPLELVEKLCPEASDIATSVRNLPELKTAVGR GRAWLYLALMQKKLADYLKVLIDNKQLLSEFYEPEALMMEEEGMVIVGLLVGLNVLDANLCLKGEDLDSQ VGVIDFSLCLKDAQDLDSGREHERITDVLDQKNYVEELNRHLSCTVGDLQTKIDGLEKTNSKLQEELSAA TDRICSLQKEQQQLREQNEVIRERSEKSVEITKQDTKVELETYKQTRQGLDEMYSDVWKQLKEEKKVRLE LEKELELQIGMKTEMEIAMKLLEKDTHEKQDTLVALRQQLEEVKAINLQMFHKVQSAESSLQQKNEAIAS FEGKTTQVMSSMKQMEERLQQAERARQAAEERSHKLQQELSGRGSALQLQLSQLRDQCSGLEKELKSEKE QRQALQRELQREKDTSCLLQTELQQVEGLKKELRELQDEKAELRKVCEEQEQALQEMGLHLSQSKLKMED IKEVNKALKGHTWLKDDEATHCKQCEKDFSISRRKHHCRNCGHIFCNTCSSNELALPSYPKPVRVCDSCH TLLLQRCSSTAS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_172557 |
ORF Size | 2136 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_172557.3 |
RefSeq Size | 2657 bp |
RefSeq ORF | 2139 bp |
Locus ID | 216724 |
UniProt ID | Q8BIJ7 |
Gene Summary | Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC221040 | Rufy1 (untagged) - Mouse RUN and FYVE domain containing 1 (Rufy1), (10ug) |
CNY 9,220.00 |
|
MR219869 | Rufy1 (Myc-DDK-tagged) - Mouse RUN and FYVE domain containing 1 (Rufy1) |
CNY 5,216.00 |
|
MR219869L3 | Lenti ORF clone of Rufy1 (Myc-DDK-tagged) - Mouse RUN and FYVE domain containing 1 (Rufy1) |
CNY 7,700.00 |
|
MR219869L4 | Lenti ORF clone of Rufy1 (mGFP-tagged) - Mouse RUN and FYVE domain containing 1 (Rufy1) |
CNY 7,700.00 |