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Spam1 (NM_009241) Mouse Tagged ORF Clone
CAT＃: MG219759
- TrueORF®
Spam1 (tGFP-tagged) - Mouse sperm adhesion molecule 1 (Spam1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_009241" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AV039194; Ph-20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219759 representing NM_009241
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGAGTTGAGATTTAAGCACCTCTTTTGGGGGAGCTTTGTTGAATCTGGGGGCACATTCCAAACAG TGTTAATCTTCCTTTTGATTCCATGCTCCTTGACTGTGGATTATAGGGCAGCACCAATTTTATCAAATAC AACTTTCCTTTGGATTTGGAATGTCCCAACTGAACGTTGTGTAGGAAATGTTAATGATCCAATAGATCTG AGCTTCTTCTCTTTAATTGGAAGCCCCCGGAAAACTGCCACAGGGCAACCTGTCACATTATTTTATGTTG ATCGACTTGGTTTGTATCCTCACATAGATGCAAACCAAGCAGAACATTATGGAGGAATACCTCAGAGGGG CGATTATCAAGCTCATTTGCGCAAAGCTAAGACTGACATAGAGCATTACATTCCAGACGACAAATTGGGC TTAGCTATCATTGACTGGGAAGAATGGAGGCCTACCTGGTTGAGAAACTGGAAACCTAAGGATAACTACA GGAATAAGTCTATTGAATTGGTCCAATCAACTAATCCAGGACTTAGTATCACAGAAGCCACCCAGAAAGC CATACAACAATTTGAAGAGGCAGGAAGGAAGTTTATGGAAGGAACTTTACACCTGGGGAAATTCCTTCGA CCAAACCAGCTATGGGGTTATTATCTATTTCCTGATTGTTATAACAATAAGTTTCAAGACCCTAAGTATG ATGGGCAGTGCCCTGCTGTGGAAAAGAAAAGAAATGATAATCTTAAATGGCTATGGAAAGCAAGCACCGG CCTTTACCCATCTGTCTATTTGAAGAAAGACTTGAAGTCCAATCGACAAGCTACCCTCTATGTCCGCTAC CGAGTTGTGGAAGCTATCAGAGTGTCCAAGGTTGGGAATGCATCGGATCCAGTCCCGATTTTTGTCTATA TCCGTCTTGTTTTTACTGATCGTACCTCTGAATACCTTCTAGAGGATGACCTTGTGAATACAATTGGTGA AATTGTTGCTCTGGGTACCTCTGGAATTATAATATGGGATGCTATGAGTTTAGCACAACGTGCGGCAGGT TGCCCAATCCTACATAAATACATGCAGACGACCCTGAATCCATACATAGTCAATGTTACCCTAGCAGCCA AAATGTGCAGCCAAACACTTTGTAATGAGAAAGGCATGTGTTCAAGAAGAAAAGAAAGTTCAGATGTATA TCTTCACTTGAACCCAAGTCATTTTGATATTATGTTAACGGAAACTGGAAAGTACGAAGTTCTTGGCAAC CCCAGGGTTGGAGACTTAGAATACTTTTCTGAACATTTTAAATGCAGCTGTTTTAGCAGAATGACATGTA AGGAGACATCTGATGTAAAAAATGTACAAGACGTGAATGTGTGCGTCGGTGACAATGTTTGTATAAAAGC CAAGGTAGAACCCAACCCAGCCTTCTACCTCCTACCTGGCAAAAGCCTTCTATTTATGACAACACTTGGT CATGTGCTGTACCATCTGCCACAAGATATTTTTGTTTTTCCACGGAAGACACTAGTCAGTACTCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219759 representing NM_009241
Red=Cloning site Green=Tags(s) MGELRFKHLFWGSFVESGGTFQTVLIFLLIPCSLTVDYRAAPILSNTTFLWIWNVPTERCVGNVNDPIDL SFFSLIGSPRKTATGQPVTLFYVDRLGLYPHIDANQAEHYGGIPQRGDYQAHLRKAKTDIEHYIPDDKLG LAIIDWEEWRPTWLRNWKPKDNYRNKSIELVQSTNPGLSITEATQKAIQQFEEAGRKFMEGTLHLGKFLR PNQLWGYYLFPDCYNNKFQDPKYDGQCPAVEKKRNDNLKWLWKASTGLYPSVYLKKDLKSNRQATLYVRY RVVEAIRVSKVGNASDPVPIFVYIRLVFTDRTSEYLLEDDLVNTIGEIVALGTSGIIIWDAMSLAQRAAG CPILHKYMQTTLNPYIVNVTLAAKMCSQTLCNEKGMCSRRKESSDVYLHLNPSHFDIMLTETGKYEVLGN PRVGDLEYFSEHFKCSCFSRMTCKETSDVKNVQDVNVCVGDNVCIKAKVEPNPAFYLLPGKSLLFMTTLG HVLYHLPQDIFVFPRKTLVSTP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009241 |
| ORF Size | 1536 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009241.3, NP_033267.2 |
| RefSeq Size | 2096 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 20690 |
| UniProt ID | P48794 |
| Gene Summary | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217290 | Spam1 (untagged) - Mouse sperm adhesion molecule 1 (Spam1), transcript variant 2, (10ug) |
CNY 6,650.00 |
|
| MR219759 | Spam1 (Myc-DDK-tagged) - Mouse sperm adhesion molecule 1 (Spam1), transcript variant 2 |
CNY 3,816.00 |
|
| MR219759L3 | Lenti ORF clone of Spam1 (Myc-DDK-tagged) - Mouse sperm adhesion molecule 1 (Spam1), transcript variant 2 |
CNY 6,370.00 |
|
| MR219759L4 | Lenti ORF clone of Spam1 (mGFP-tagged) - Mouse sperm adhesion molecule 1 (Spam1), transcript variant 2 |
CNY 6,370.00 |
