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Madd (NM_001177719) Mouse Tagged ORF Clone
CAT＃: MG219737
- TrueORF®
Madd (tGFP-tagged) - Mouse MAP-kinase activating death domain (Madd) transcript variant 10, (10ug)
ORF Plasmid: DDK
"NM_001177719" in other vectors (3)
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CNY 17,390.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9630059K23Rik; IG20 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219737 representing NM_001177719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGCAAAAGAAGTTCTGCCCTCGGTTACTTGACTATCTCGTGATCGTAGGGGCGAGGCACCCAAGCA GTGACAGTGTGGCTCAGACTCCTGAGCTGCTGCGGAGGTACCCACTAGAGGATCACCCAGAGTTCCCCCT GCCCCCAGATGTGGTGTTCTTCTGCCAGCCAGAAGGATGTCTGAGTGTGCGGCAGCGGCGGATGAGCCTT CGGGACGATACCTCTTTTGTCTTCACCCTAACCGATAAGGACACCGGAGTCACCCGCTATGGCATCTGTG TCAACTTCTACCGTTCCTTCCAAAAGCGAATGCCAAAGGAAAAGGTGGAAGGCGGAGCAGGACCCCGTGG GAAGGAAGGCGCTCACACCTCTGGTGCCTCAGAAGAGGCTGCCGCTGGGAGCTCAGAGAGTGGCTCAACC TTGCAGCCGCCTAGTGCTGACTCCACTCCTGACGTAAACCAGTCTCCTCGGGGCAAACGTAGGGCAAAAG CGGGCAGCCGCTCCCGCAACAGTACCCTGACATCCCTGTGTGTGCTTAGCCACTACCCTTTCTTCTCTAC CTTCAGAGAGTGTCTGTATACTCTCAAACGTTTGGTAGACTGCTGTAGTGAACGGCTGCTAGGCAAGAAA CTGGGCATCCCTCGAGGTGTACAAAGGGACACCATGTGGCGAATCTTTACTGGATCACTGCTAGTGGAGG AGAAGTCCAGTGCCCTTCTGCAAGACCTTCGAGAGATTGAGGCCTGGATCTATCGGTTGCTACGTTCCCC AGTGCCTGTCTCTGGGCAGAAGCGAGTGGACATTGAGGTCCTGCCCCAGGAACTCCAGCAGGCCCTGACA TTTGCTCTTCCAGATCCATCTCGATTCACCCTAGTAGATTTCCCTCTTCACCTTCCCTTGGAACTTCTGG GTGTAGATGCTTGTCTTCAGGTGCTAACCTGCATCCTGTTGGAGCACAAGGTGGTGCTGCAGTCTCGAGA CTACAATGCACTCTCCATGTCTGTGATGGCGTTCGTGGCAATGATCTACCCCCTGGAGTACATGTTCCCG GTCATCCCGCTGCTTCCCACCTGCATGGCCTCGGCAGAGCAGCTACTCTTGGCTCCAACCCCATACATCA TTGGAGTTCCTGCCAGCTTCTTCCTCTACAAGCTAGATTTCAAAATGCCCGATGACGTGTGGCTAGTGGA TCTGGACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTACCCATCCTGCCAGAACCAGAATCA TTAGAGCTGAAAAAACACTTAAAGCAGGCCTTAGCCAGCATGAGTCTCAACACCCAGCCCATTCTTAACC TGGAGAAATTCCACGAGGGCCAGGAGATCCCGCTTCTCTTGGGAAGGCCATCTAATGACCTTCAGTCCAC ACCTTCCACTGAATTCAACCCGCTCATTTATGGCAATGATGTAGATTCAGTCGACGTTGCAACGAGAGTG GCCATGGTACGTTTCTTCAACTCTGCTAACGTGCTGCAGGGCTTTCAGATGCACACACGTACCCTGCGAC TCTTCCCCCGGCCTGTGGTAGCTTTCCAAGCTGGCTCCTTTCTGGCCTCACGTCCCCGGCAGACTCCCTT TGCTGAGAAACTGGCCAGGACCCAAGCTGTGGAGTATTTTGGAGAATGGATCCTGAACCCTTCTAACTAT GCCTTCCAGCGGATTCACAACAATACGTTTGATCCGGCCCTTATTGGTGACAAGCCAAAGTGGTACGCTC ACCAGCTGCAGCCCATCCATTATCGAGTCTATGATGGCAACTCTCAGCTGGCCGAGGCCCTGAGTGTGCC CCCGGAGCGCGATTCTGACTCCGACCCGACGGAGGACAGTGGCAGTGATAGTCAGGATTATGATGACTCA AGCTCTTCTTACTCCTCCCTTGGGGACTTTGTCAGTGAAATGATGAAATGTGACATCAATGGAGACACTC CCAATGTGGATCCTCTGACCCATGCAGCCCTGGGAGATGCCAGTGAAGTGGAGATTGACGAGCTGCAGCC CCAGAAGGAGGGAGAGGAACCCGGCCCAGACAGTGAGAACTCTCAGGAAAACCCTCCGCTGCGCTCCAGC TCCAGCACCACGGCCAGCAGCAGCCCCAGCACCGTTGTCCACAGTGCCCACTCTGAAGCTGCTGACTCTA CAGAGATGGGAGATAAGGCCACAGCAGGCATCTCCAAGCCCCTCCCTCCCGTGCCTCCCAGCATTTGCAA ATCTACTGTGGACAGGAGACAGACAGAGACTGGAGAGGGGTCAGTGTGCCAGCGAACCTATGACAATCCA TACTTCGAGCCCCAGTATGGCTTTCCCCCCGAGGAAGATGAGGAGGAGCAGGGGGAGAGCTACACTCCCC GGTTCAGCCAGCATGTCAGTGGCAGTCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAAGCTGGCAAG TGACTCAGACGCAGAGTCAGACTCGAGAGCAAGCTCTCCCAACTCCACTGTCTCTAACAACAGCACCGAG GGCTTCGGGGGCATCATGTCTTTTGCTAGCAGCCTGTATCGGAACCATAGTACAAGCTTCAGTCTTTCAA ACCTCACACTGCCCACCAAAGGTGCCCGCGAGAAGACGACACCATTCCCCAGCCTGAAAGTATTTGGGCT AAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCTGGGAGTGGTGAAGGAAATAGGAGGGCCTTAGTG GACCAGAAGTCATCTGTCATTAAACACAGCCCAACCGTGAAAAGAGAACCTTCATCCCCACAGGGCCGGT CGAGCAACTCTAGCGAGAACCAGCAGTTCCTGAAGGAAGTAGTCCATAGTGTGCTGGACGGCCAGGGAGT GGGCTGGCTCAATATGAAGAAGGTACGCCGGCTCCTGGAGAGTGAGCAGCTTAGAGTCTTTGTACTGAGC AAGCTGAATCGCGCAGTGCAGTCAGAGGATGACGCCCGGCAGGATGTCATCCAAGACGTGGAGATCAGCC GGAAGGTGTACAAGGGGATGCTAGACCTCCTCAAGTGCACAGTCCTCAGCCTCGAGCAGTCCTATGCCCA CGCAGGTCTGGGTGGTATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAA GAACCAGACAAACGGAAGAGAAGTCCGACAGAGAATGTAAATACCCCAGTTGGCAAGGATCCTGGTCTGG CTGGGCGGGGGGACCCAAAGGCAATGGCCCAGCTAAGGGTCCCTCAACTGGGCCCTCGGGCACCAAGTGC TACAGGAAAGGGTCCTAAAGAACTGGATACCAGAAGTTTAAAGGAAGAGAATTTTGTAGCATCTGTTGAA TTGTGGAACAAGCACCAGGAAGTGAAAAAGCAAAAGGCTTTGGAAAAACAGAGGCCAGAGGGCATCAAGC CTGTCTTTGACCTGGGTGAGACAGAGGAGAAAAAGTCCCAGATGAGCGCAGACAGTGGTGTGAGCCTGAC ATCTGCCTCCCAGAGGACTGATCAAGACTCTGTCATCGGTGTGAGTCCAGCTGTTATGATCCGAAGCTCA AGTCAGGATTCTGAAGTTAGCACCGTGAGTAATAGTTCCGGAGAGACCCTTGGAGCTGACAGTGACCTGA GCAGCAATGCAGGTGACGGGCCAGGAGGCGAAGGCAGCGCCCACCTGGCAAGTTCCCGGGCCACTCTGTC CGACAGTGAAATCGAAACCAATTCTGCTACAAGCGCCATCTTTGGTAAAGCCCATAGCTTGAAGCCAAAG GAGAAGCCGGCTGGTAGTCCGATTCGCTCTTCCGAAGACGTAAGCCAGCGAGTCTATCTCTATGAGGGAC TGCTAGGAAGGGACAAAGGATCCATGTGGGACCAGCTAGAGGATGCCGCTATGGAGACCTTTTCTCTGAG CAAAGAACGTTCTACCTTATGGGACCAAATGCAGTTCTGGGAAGATGCATTCTTAGATGCTGTAATGTTG GAAAGAGAAGGAATGGGTATGGACCAGGGTCCTCAGGAAATGATCGACAGGTACCTCTCCCTAGGGGAAC ACGACCGGAAGCGCCTGGAGGATGATGAAGACCGCTTGCTGGCCACACTTTTACACAACCTCATATCCTA TATGCTGCTGATGAAGGTGAACAAGAATGACATCAGGAAGAAAGTACGGCGCCTAATGGGAAAGTCCCAC ATTGGGCTGGTGTACAGCCAGCAAGTCAATGAGGTGCTCGATCAGCTGAACAGCCTGAATGGACGTGATC TCTCCATCCGGTCCAGCGGCAGCCGGCACATGAAGAAGCAGACTTTTGTTGTGCATGCAGGGACGGACAC AAATGGAGACATCTTTTTCATGGAAGTGTGTGATGACTGCGTGGTGTTGCGTAGTAACATTGGGACGGTG TACGAGCGCTGGTGGTATGAGAAGCTCATCAATATGACCTACTGTCCCAAGACCAAGGTCCTGTGCTTAT GGCGGAGAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACCAAGAAGTGTCGAGAGCTGTACTACTG TGTGAAGGACAGCATGGAACGTGCTGCTGCCCGACAGCAAAGCATCAAACCTGGGCCTGAGCTAGGCGGT GAGTTCCCTGTGCAGGACATGAAGACTGGAGAGGGTGGCTTGCTTCAAGTCACCCTGGAAGGGATCAATC TCAAGTTCATGCACAACCAGTTCCTGAAATTAAAGAAGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219737 representing NM_001177719
Red=Cloning site Green=Tags(s) MVQKKFCPRLLDYLVIVGARHPSSDSVAQTPELLRRYPLEDHPEFPLPPDVVFFCQPEGCLSVRQRRMSL RDDTSFVFTLTDKDTGVTRYGICVNFYRSFQKRMPKEKVEGGAGPRGKEGAHTSGASEEAAAGSSESGST LQPPSADSTPDVNQSPRGKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCCSERLLGKK LGIPRGVQRDTMWRIFTGSLLVEEKSSALLQDLREIEAWIYRLLRSPVPVSGQKRVDIEVLPQELQQALT FALPDPSRFTLVDFPLHLPLELLGVDACLQVLTCILLEHKVVLQSRDYNALSMSVMAFVAMIYPLEYMFP VIPLLPTCMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNRVIAPTNAEVLPILPEPES LELKKHLKQALASMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRV AMVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQTPFAEKLARTQAVEYFGEWILNPSNY AFQRIHNNTFDPALIGDKPKWYAHQLQPIHYRVYDGNSQLAEALSVPPERDSDSDPTEDSGSDSQDYDDS SSSYSSLGDFVSEMMKCDINGDTPNVDPLTHAALGDASEVEIDELQPQKEGEEPGPDSENSQENPPLRSS SSTTASSSPSTVVHSAHSEAADSTEMGDKATAGISKPLPPVPPSICKSTVDRRQTETGEGSVCQRTYDNP YFEPQYGFPPEEDEEEQGESYTPRFSQHVSGSRAQKLLRPNSLKLASDSDAESDSRASSPNSTVSNNSTE GFGGIMSFASSLYRNHSTSFSLSNLTLPTKGAREKTTPFPSLKVFGLNTLMEIVTEAGPGSGEGNRRALV DQKSSVIKHSPTVKREPSSPQGRSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFVLS KLNRAVQSEDDARQDVIQDVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQTHYYSK EPDKRKRSPTENVNTPVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKEENFVASVE LWNKHQEVKKQKALEKQRPEGIKPVFDLGETEEKKSQMSADSGVSLTSASQRTDQDSVIGVSPAVMIRSS SQDSEVSTVSNSSGETLGADSDLSSNAGDGPGGEGSAHLASSRATLSDSEIETNSATSAIFGKAHSLKPK EKPAGSPIRSSEDVSQRVYLYEGLLGRDKGSMWDQLEDAAMETFSLSKERSTLWDQMQFWEDAFLDAVML EREGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDIRKKVRRLMGKSH IGLVYSQQVNEVLDQLNSLNGRDLSIRSSGSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTV YERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRELYYCVKDSMERAAARQQSIKPGPELGG EFPVQDMKTGEGGLLQVTLEGINLKFMHNQFLKLKKW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177719 |
| ORF Size | 4731 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177719.1, NP_001171190.1 |
| RefSeq Size | 6151 bp |
| RefSeq ORF | 4734 bp |
| Locus ID | 228355 |
| UniProt ID | Q80U28 |
| Gene Summary | Plays a significant role in regulating cell proliferation, survival and death through alternative mRNA splicing. Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms. Component of the TNFRSF1A signaling complex: MADD links TNFRSF1A with MAP kinase activation. Plays an important regulatory role in physiological cell death (TNF-alpha-induced, caspase-mediated apoptosis).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR219737 | Madd (Myc-DDK-tagged) - Mouse MAP-kinase activating death domain (Madd), transcript variant 10 |
CNY 15,770.00 |
|
| MR219737L3 | Lenti ORF clone of Madd (Myc-DDK-tagged) - Mouse MAP-kinase activating death domain (Madd), transcript variant 10 |
CNY 17,670.00 |
|
| MR219737L4 | Lenti ORF clone of Madd (mGFP-tagged) - Mouse MAP-kinase activating death domain (Madd), transcript variant 10 |
CNY 17,670.00 |
