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Rapgef1 (NM_001039086) Mouse Tagged ORF Clone
CAT＃: MG219461
- TrueORF®
Rapgef1 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001039086" in other vectors (4)
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CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4932418O06Rik; C3G; Grf2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219461 representing NM_001039086
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAGCGGCCTCGGCCTCCGGCGCAGCCCGGAAATGTCCGGCAAGATCGAGAAAGCAGACTCTCAGC GTTCTCATCTCTCCTCCTTCACCATGAAGCTGATGGACAAATTCCACTCTCCCAAAATCAAGAGAACACC ATCCAAGAAGGGAAAGCCAGCCGAGGTGTCGAAGATTCCTGAGAAGCCTGTGAGCAAAAACCTGTGCTGG CTGGAGGAGAAAGAGAAGGAGGTTGTCAGTGCCTTGCGCTACTTTAAGACCATTGTGGACAAAATGGCCA TTGATAAGAAGGTTCTGGAGATGCTCCCGGGGTCAGCCAGCAAGGTGCTGGAGGCCATCTTACCCCTGGT GCAGACTGACCCTCGGATCCAGCACAGCTCAGCCCTCTCCTCCTGTTATAGCCGAGTATACCAGAGCCTC GCCAACCTTATCCGATGGTCTGACCAGGTGATGCTAGAGGGCGTGAACTCAGAAGATAAGGAGATGGTGA CAACTGTGAAGGGCGTTATCAAAGCTGTCCTGGACGGAGTGAAGGAGCTAGTGAGGCTAACCATTGAGAA GCAGGGGCGGCCATCGCCAACAAGCCCAGTGAAGCCCAGTTCCCCAGCCAGCAAGCCTGATGGCCAGCCT GAGCTCCCTCTGACAGACCGAGAAATGGAGATTCTGAACAAGACGACAAGTGTGTCACCATCTGCTGAAC TGCTCCCAGACTCCACCAGTGAAGAGGTCGCACCCCCCAAGCCCCCTTTACCTGGCATCCGGGTGGTTGA TAACAGTCCACCAGCATTACCACCCAAGAAAAGGCAGTCTGCTCCATCCCCCACTCGGGTGGCTGTGGTA GCCCCAATGAGTCGGGCTACCAGTGGCTCCAGTTTGCCTGTTGGAATCAATAGGCAGGACTTTGATGTTG AATGTTACACCCAGAGGCGCCTGTCAGGAGGCAGCCGCTCCTGCGGTGGTGAGTCTCCTCGCCTGTCCCC CTGCAGCAGCACAGGCAAGCTCAGCCGCTCAGACGAGCAGCTGTCCTCCCTGGACAGGGATAGTGGGCAG TGCTCACGGAACACAAGCTGTGAAACACTAGATCACTACGACCCCGACTATGAATTCCTCCAGCAAGATC TCTCCAATGCAGACCAGATCCCTCCACAGGCAGCCTGTAACCTCAGCCCTCTGCCGGAGTCCCTGGGGGA ATCTGGGCCTCCATTTCTTGGCCACCCTTTCCAGCTGCCTTTGGGCAGCTGTCTGCAGCAGGAGGGACAG CAGACAGACACTCCACCTGCCCTTCCGGAGAAGAAGCGTAGGAGCGCAGTCTCCCAGACCACGGACAGCT CTGGCTGCAGGGTGTCCTATGAGCGACACCCCTCACAGTATGACAACATCTCAGAGGGTGACCTGCAGAA CCCAGTCCCAGTCCAGCCTGTGCCCTACCCACCCTTTGCTGCTGTCCTGCCCTTTCAGCAGGGAGCTTCC TCTGCCTCTGCTGAGTTTGTGGGTGATTTCAGTGTTCCTGAGTTGGCGGGTGACACAGAGAAGCCACCTC CTCTACCAGAGAAGAAGAACAAGCACATGCTGGCCTACATGCAACTGCTGGAGGACTACTCAGAGCCACA GCCCTCCATGTTCTACCAGACACCGCAGAGTGAGCACATCTACCAGCAGAAGAACAAGATGCTCATGGAG GTGTACGGCTTCAGCGAGTCCTTCTGCGGTAGTGATTCCACGCAGGAGCTGGCCCCTCCACCCGCTCTGC CCCCCAAGCAACGGCAGCTGCAGGCCTCCTATGCTGCTTCTTCCTTCTCTGTCTCCTACTGTGTCCAGCA AACTAAAGTGGCCTTCACCCCCGAGGACGGCAGTGCCGCTCAGGGCCTCAGTGTATCCGTGTCTAACTCC TTTCTCAACCGGCACGGCAGCTTGCCTGTGCCCTCGTACAAATCTGTGTTTAGGTCTTACTCCCAGGACT TCATGCCTCACCACCAAGCCTCCGTCCAACCTTTCCTTCCGCCTACCTCCTCTTCCTCTCCACATTTCCC ACCTGTCCACACGTCCCAGAGCTCGGACTTAGCGGTGCCCACCGTAAGCAGTCCGCCTCCCAGCACCGTG GACGGGCCTCTCTCGTCTTCTCAGGACAGCAGCTTTCATGGGAACCCTGTCCGCCTTCCTTCCGAAACCT CTTTCACTGACTCGAGTGAAAAGGCCAGCAGTGAGGAAGCTGGTGGGGATGAGTATGTCAGTCTGTACTC CTCTGGCCAGACCAGCGAGGAGCTGGCTCCCTGTCGAGGAGAACCACCATCTGGGAAGGACGGACATCCC AGAGATCCTTCGGTCAGCAGTGCATCTGGGAAGGACAGCAGAGAAAATGGGGAAAGGTCCCCAAAGTCAC TGGATGGTCTGGAGTCAGCGCAGTCAGAAGAGGAAGTGGATGAACTGTCCCTCATTGACCACAATGAAAT TATGGCCAGGCTGACACTCAAGCAAGAGGGTGATGATGGGCCAGATGTTCGTGGTGGTTCAGGAGACATC TTACTGGTCCATGCTACTGAGACAGACAGAAAAGACCTGGTGCTGTACTGTGAAGCCTTCCTGACCACCT ACAGGACCTTCATCAGCCCGGAGGAGCTCATCAAGAAGCTGCAGTACCGGTACGAGAAGTTCTCTCCCTT TGCTGACACGTTCAAGAAGCGAGTGAGCAAGAACACATTCTTTGTGCTGGTTCGAGTGGTGGATGAGCTC TGCCTGGTGGAGCTGACAGAGGAGATCCTGAAGCTGCTGATGGAGCTGGTCTTCCGCCTAGTGTGCAGCG GAGAGCTCAGCCTGGCCAGAGTCCTCCGGAAGAACATTCTGGACAAGGTGGACCAGAAGAAGCTGCTCAG GTGTGCCCATTCCGACCAGCCTCTGGCAGCCAGGGGTGTTGCAGCCAGGCCAGGAACCTTGCATGATTTC CACAGCCACGAGATAGCTGAGCAGCTGACACTGCTGGATGCCGAGCTTTTCTACAAGATAGAGATTCCTG AAGTTTTGCTTTGGGCCAAAGAGCAGAATGAGGAGAAGAGTCCCAATCTGACCCAGTTCACAGAGCACTT CAACAACATGTCCTACTGGGTGCGGTCCATCATCATGCTGCAGGAGAAGGCCCAGGACCGGGAGAGGCTG CTCCTCAAGTTCATCAAGATCATGAAGCACCTGCGCAAGCTCAACAACTTCAACTCCTACCTGGCCATCC TCTCAGCACTAGACTCGGCCCCCATCCGCAGACTGGAGTGGCAGCGGCAGACCTCAGAGGGCCTGGCTGA GTACTGCACATTGATTGACAGCTCATCCTCCTTCCGAGCCTACCGGGCTGCTCTCTCAGAGGTGGAGCCC CCATGTATCCCATACCTAGGTCTGATTCTGCAAGACCTGACCTTCGTTCACCTGGGAAACCCAGATTATA TTGACGGGAAAGTAAACTTCTCCAAGCGGTGGCAACAGTTCAACATATTGGACAGCATGCGGTGCTTCCA GCAGGCGCACTATGAAATCCGGAGAAACGACGACATCATAAATTTCTTCAATGACTTCAGTGACCACCTG GCCGAGGAAGCCCTGTGGGAACTCTCTCTGAAGATCAAGCCTAGGAACATAACAAGGCGGAAAACAGACC GCGAAGAGAAGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219461 representing NM_001039086
Red=Cloning site Green=Tags(s) MSSGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSKIPEKPVSKNLCW LEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQTDPRIQHSSALSSCYSRVYQSL ANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGQP ELPLTDREMEILNKTTSVSPSAELLPDSTSEEVAPPKPPLPGIRVVDNSPPALPPKKRQSAPSPTRVAVV APMSRATSGSSLPVGINRQDFDVECYTQRRLSGGSRSCGGESPRLSPCSSTGKLSRSDEQLSSLDRDSGQ CSRNTSCETLDHYDPDYEFLQQDLSNADQIPPQAACNLSPLPESLGESGPPFLGHPFQLPLGSCLQQEGQ QTDTPPALPEKKRRSAVSQTTDSSGCRVSYERHPSQYDNISEGDLQNPVPVQPVPYPPFAAVLPFQQGAS SASAEFVGDFSVPELAGDTEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQSEHIYQQKNKMLME VYGFSESFCGSDSTQELAPPPALPPKQRQLQASYAASSFSVSYCVQQTKVAFTPEDGSAAQGLSVSVSNS FLNRHGSLPVPSYKSVFRSYSQDFMPHHQASVQPFLPPTSSSSPHFPPVHTSQSSDLAVPTVSSPPPSTV DGPLSSSQDSSFHGNPVRLPSETSFTDSSEKASSEEAGGDEYVSLYSSGQTSEELAPCRGEPPSGKDGHP RDPSVSSASGKDSRENGERSPKSLDGLESAQSEEEVDELSLIDHNEIMARLTLKQEGDDGPDVRGGSGDI LLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDEL CLVELTEEILKLLMELVFRLVCSGELSLARVLRKNILDKVDQKKLLRCAHSDQPLAARGVAARPGTLHDF HSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERL LLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQRQTSEGLAEYCTLIDSSSSFRAYRAALSEVEP PCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYEIRRNDDIINFFNDFSDHL AEEALWELSLKIKPRNITRRKTDREEKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039086 |
| ORF Size | 3654 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039086.1, NP_001034175.1 |
| RefSeq Size | 6233 bp |
| RefSeq ORF | 3657 bp |
| Locus ID | 107746 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223980 | Rapgef1 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2, (10ug) |
CNY 15,580.00 |
|
| MR219461 | Rapgef1 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2 |
CNY 12,160.00 |
|
| MR219461L3 | Lenti ORF clone of Rapgef1 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2 |
CNY 14,060.00 |
|
| MR219461L4 | Lenti ORF clone of Rapgef1 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 1 (Rapgef1), transcript variant 2 |
CNY 14,060.00 |
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