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Aff1 (NM_133919) Mouse Tagged ORF Clone
CAT＃: MG219346
- TrueORF®
Aff1 (tGFP-tagged) - Mouse AF4/FMR2 family member 1 (Aff1) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_133919" in other vectors (4)
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CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9630032B01Rik; Af; Af4; AW319193; Mllt; Mllt2h; R; Rob |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219346 representing NM_133919
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCCATTCCAGCTTGTACAATGAAGATAGAAACCTGCTTCGAATCAGAGAGAAGGAAAGACGCA ACCAAGAAGCTCACCAGGAGAAGGAGGCATTTCCCGAGAAGGCTCCCCTGTTTCCAGAGCCTTACAAGAC TGCAAAAGGCGATGAGCTATCAAGTCGGATCCAGACCATGCTGGGTGACTATGAGGAGATGAAGGAGTTC CTGAGCAGCAAGTCCCACCCCCACCGCCTGGATGGCTCTGAAGACAGGCCCGGGAAGCCCAGATATCCCT TAGGTCATGACAGGGGGAACGGGGCTGCATCCAGCTCCCTCCGCACACATGTCTACCACCAGCCTATCCA CACTTCTGCTCCCGGATCACGTCCTGTCGGTAACATTAGCCACAGTCCAAAGATGGCACAGCCAAGGATG GAGCCAAGTCTCCACACCAAAATCTATGATGGCCCACGTCTGACTCAAGACCACCTCAGTCAGGGACATT GTTCCAGAAAGTGTGACCGAAGAGCTGAAGGAGACTCTGCTCCCGAGAGGAAGCTCTCGCCCTTGATCTC CTCTTTGCCATCCCCGGTGCCCCCTCTATCACCTGTACATTCCAGGCTGCAGGGAACCAGCAAGGCTCAC AGCAGTGGCGTTAGCAGTAAAAGCTGCTGTGTGGCCAAGTCTTCCAAGGACTTGGTGGCGAAGGCCCAAG ATAAAGAGACTCCTCATGACGGTTTGGTGGCAGTTACCAGCCTTGGATCAGCCCCTCCTCAGCCACCTTG CCAGACATTTCCACCCCCTCCTCTCCCCTCAAAAAGTGCTGCAATGCAGCAGAAGCCCACGGCATATGTC CGTCCCATGGACGGTCAGGATCAGGCTCCCAGTGAGTCCCCCGAGCTGAAGCTGCCACTGGAGGACTATG GTCAGCAAAGCTTTGAGAAACCAGACCTTAAAGTGCCTGCCAAAGCCAAGCTCACCAGACTAAGGATGCC CTCTCAGTCGGTGGAGCAGCCGTACTCCAATGAAGTCCATTGCGTTGAAGAGATTCTGAAGGAAATGACC CACTCGTGGCCACCGCCTCTGACAGCCATACACACACCCAGTACAGCCGAGCCGTCCAGATTTCCTTTCC CCACAAAGGACCCTCTGCACGTCAGTCCTGCAACCCAGAGCCAAAAACAATATGACACGCCTTCAAAAAC CCACCCCAATCCCCAGCAAGGAACCTCCATGCTGGAGGACGACCTGCAGCTCAGCGACAGTGAAGACAGC GACACGGAGCAAGCCACAGAGAAGCCTCCATCCCCACCTGCACCTCCCAGCGCTCCGCAGACACTTCCCG AGCCCGTGGCCTCAGCACATTCCAGTAGTGGGGAGTCAGAGAGCAGCGAGTCAGACAGTTCCTCAGACTC TGAGAGTGAGAGCAGCTCCAGCGACAGCGAGGAGGAGGAAGAGAACGAGCCCCTGGAGACCCGGGCTCCC GAGCCCGAGCCTCCGACGACAAACAAATGGCAGCTGGACAACTGGTTGACCAAAGTCAACCAACCCTCAG TGCCCCTGGATGGCCGGGGGAGCACAGAGTCTCCACAATGGCGCCAGGAAAGTAAGGGTGTCGCTGAGGG CAGCAGTGACCAGCAGCATCCTGATTCCAAAGACCCTCTCCCAAAGAGCTCCAGCAAGACTCTCCGGGGT CCCTCTGAGGGGCCCCAGCCTGGGAAGAGGGGCTGTCCGAAATCCCCCGCTCAACAGGAACCCCCACCTC GGCAAACCGTGGGCAGCAAACAACCCAGGAAACCCGCCAAGGGCTCCGGCCAGGCAGAGCCCCAGGCCAG CTCGCAGGCAGAGAGTGAAGTAGGACCCCTTCCCTACGGCTCAAAGGAGCAGACTTCCAAAGACAGACCC AAGGTGAAGACGAAAGGCAGGCCGCGTGCCGTGGGCAGTCGGGAGCCTAAGCCAGAGGTCCCTGCGCCCA CGCCTCAGGCAGCCGTGCCCAGACCGAAGCCGCCTGTGCCCACCCCCAGTGAGAAGAGGAAGCACAAGAG CTCCACGGCCCCCTCCAAGGCGCCCTCAGCCCCCCAGCCTCCGAAGGACAGTGCTGGGGACAGGAACCCA GAACACTCTGCTCTTGTCTCCTTGACTCAGAGCCAGGGTCCATCCCACAGCAGCAGGGGCAGCAGTGGCA GCGTCAGGACTAGTGGCTGCCGACAGGCTGTGATTGCCCAAGGGGACGGCTGTAAAGACAAGCTCCTGTT GCCTTTGAGAGACACCAAATTGCTCTCGCCGCTCAGGGACTCCCCACCACCGACAAGCTTGGTGGTGAAG ATAACCCTAGACCTCCTCACGAGGATTCCCCAGCCTCTGGGGAAGGGGAGCCGCCCCAGGAAAGCAGAAG ACAAGCAGCTGTCAGCAGGGAAGAAGCAGGACTCGGAGACCAAGAGCTGTGACAGCTCCAGCAGAGTGAC CAAGAAGAGAAAGGGTGACCCAGAAAAAGAGCACAGTAACAAGAGACACAAACTGGATAAGTCGCAGACT GCGTCATCTTCATCTTCCCATACAGAATCTTCCAGAACAAAAGCGCCCAGGTCCTCCTCAGAGAACTCAA GAAAGGAAATGCTGCCTCCAGCATCGGCGTCCTCAGTCTCCTCTTCCTCATCCTCCCAGAAGCCAAGCAG ACCTGCACAGAAGAGGCCAAGGCCAGACGAAGACACGTGTAGCCAGGAACCCCCCAGAAGTGCCAGCAGC ACAAAGAGCAGCTCTACAGACCCTCCCGCTCCCAAGCACAGAAAAGTACAGGCCAGGGGCTCTGAACACA AGGGATCTTCTGGGGATGCTGCGAATGCTGCAAATCCTTTTCCAGTGCCTTCTTTGCCGAATGGTAACGC TAAACCAGGGAAGCCACAGGTGAAGTCTGACAGACAACAAGCTGACTTTCACATGAAGGAGGCTAAAAAG CTGAAGTGCAAAGCAGAGACAATGGTAGACAAGGCTGGAAAAGCCTTCAAGTATTTGGAAGCCGTCCTCT CTTTTATCGAGTGTGGAATGGCCTCAGAGTCAGAAAGCTCAGCCAAGTCGGCGTACGCTGTGTACTCAGA AACCATAGACCTCATTAGGTATGTGATGTCACTAAAATGCTTCTCAGATAACACGATGCCAGCACAAGAG AAGATATTTGCTGTTTTATGCTTGCGTTGCCAGTCCCTTTTGAACATGGCGATGTTTCGATGTAAGAAAG ACACAGTGATGAAGTATTCTCGTACTCTCAGTGAACACTTCAAGAGTACTTCCAAAGTGGCCCAGGCACC TTCTCCATGCACGGCAAGAAGCACAGGCGTTCCGTCCCCCCTCTCCCCAATGCCTTCTCCTGCCAGCTCC GTGGGGTCCCAATCAAGTGCTGGCAGCAGTATGGGGAGCGTCGGGGTGACGGCCACCGTCAGTACTCCAG TCTCCATCCAGAACATGACCTCCTCCTATGTCACCATCACATCCCATGTCCTTACGGCCTTTAGCCTTTG GGAACAGGCCGAGGCCCTCACAAGGAAGAACAAAGAATTCTTCGCTCAGCTCAGCACAAAAGTGCGTGTG TTGGCCCTCAACAGCAGCCTGGTGGACCTGGTGCACTACACAAGACAGGGCCTGCAGCGGCTAAAACAGT CACCCAAAGGCCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219346 representing NM_133919
Red=Cloning site Green=Tags(s) MAAHSSLYNEDRNLLRIREKERRNQEAHQEKEAFPEKAPLFPEPYKTAKGDELSSRIQTMLGDYEEMKEF LSSKSHPHRLDGSEDRPGKPRYPLGHDRGNGAASSSLRTHVYHQPIHTSAPGSRPVGNISHSPKMAQPRM EPSLHTKIYDGPRLTQDHLSQGHCSRKCDRRAEGDSAPERKLSPLISSLPSPVPPLSPVHSRLQGTSKAH SSGVSSKSCCVAKSSKDLVAKAQDKETPHDGLVAVTSLGSAPPQPPCQTFPPPPLPSKSAAMQQKPTAYV RPMDGQDQAPSESPELKLPLEDYGQQSFEKPDLKVPAKAKLTRLRMPSQSVEQPYSNEVHCVEEILKEMT HSWPPPLTAIHTPSTAEPSRFPFPTKDPLHVSPATQSQKQYDTPSKTHPNPQQGTSMLEDDLQLSDSEDS DTEQATEKPPSPPAPPSAPQTLPEPVASAHSSSGESESSESDSSSDSESESSSSDSEEEEENEPLETRAP EPEPPTTNKWQLDNWLTKVNQPSVPLDGRGSTESPQWRQESKGVAEGSSDQQHPDSKDPLPKSSSKTLRG PSEGPQPGKRGCPKSPAQQEPPPRQTVGSKQPRKPAKGSGQAEPQASSQAESEVGPLPYGSKEQTSKDRP KVKTKGRPRAVGSREPKPEVPAPTPQAAVPRPKPPVPTPSEKRKHKSSTAPSKAPSAPQPPKDSAGDRNP EHSALVSLTQSQGPSHSSRGSSGSVRTSGCRQAVIAQGDGCKDKLLLPLRDTKLLSPLRDSPPPTSLVVK ITLDLLTRIPQPLGKGSRPRKAEDKQLSAGKKQDSETKSCDSSSRVTKKRKGDPEKEHSNKRHKLDKSQT ASSSSSHTESSRTKAPRSSSENSRKEMLPPASASSVSSSSSSQKPSRPAQKRPRPDEDTCSQEPPRSASS TKSSSTDPPAPKHRKVQARGSEHKGSSGDAANAANPFPVPSLPNGNAKPGKPQVKSDRQQADFHMKEAKK LKCKAETMVDKAGKAFKYLEAVLSFIECGMASESESSAKSAYAVYSETIDLIRYVMSLKCFSDNTMPAQE KIFAVLCLRCQSLLNMAMFRCKKDTVMKYSRTLSEHFKSTSKVAQAPSPCTARSTGVPSPLSPMPSPASS VGSQSSAGSSMGSVGVTATVSTPVSIQNMTSSYVTITSHVLTAFSLWEQAEALTRKNKEFFAQLSTKVRV LALNSSLVDLVHYTRQGLQRLKQSPKGP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133919 |
| ORF Size | 3654 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133919.4, NP_598680.3 |
| RefSeq Size | 8323 bp |
| RefSeq ORF | 3657 bp |
| Locus ID | 17355 |
| Gene Summary | This gene encodes a member of the AF4/ lymphoid nuclear protein related to the Fragile X E syndrome (FRAXE) family of proteins, which have been implicated in human childhood lymphoblastic leukemia, fragile chromosome X intellectual disability, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein domain, and a C-terminal homology domain. Knockout of the mouse gene by homologous recombination severely affects early events in lymphopoiesis, including precursor proliferation or recruitment, but is dispensable for terminal differentiation. In addition, an autosomal dominant missense mutation results in several phenotypes including ataxia and adult-onset Purkinje cell loss in the cerebellum, indicating a role in Purkinje cell maintenance and function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223967 | Aff1 (untagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2, (10ug) |
CNY 15,580.00 |
|
| MR219346 | Aff1 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2 |
CNY 12,160.00 |
|
| MR219346L3 | Lenti ORF clone of Aff1 (Myc-DDK-tagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2 |
CNY 14,060.00 |
|
| MR219346L4 | Lenti ORF clone of Aff1 (mGFP-tagged) - Mouse AF4/FMR2 family, member 1 (Aff1), transcript variant 2 |
CNY 14,060.00 |
