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Ppef1 (NM_011147) Mouse Tagged ORF Clone
CAT＃: MG219324
- TrueORF®
Ppef1 (tGFP-tagged) - Mouse protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), (10ug)
ORF Plasmid: DDK
"NM_011147" in other vectors (4)
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CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PPEF-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219324 representing NM_011147
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTGTGGCACTTCTTCAAAAAAAGGCAACAAATCTAAGAAAGTGATAAAAGCCGCGTTAGTCATCC AAAATTGGTACCGGCGGTACAGAGCTCGACTGAGGGTCAGACAGCATTATGCCCTCGCCATCTTCCAGTC TATTGAATATTCTGACGAGCAAGGCCAAATGCAGCTATCCAGCTTCTTTTCGTTCATGTTGGAAAACTAC ACAAAGACAAATAATGAAGATTCAGCATTAGTAACTCGAATTTTTGACAACACCAGGCGGGAAAGCCAAA TCAAGGATAGAGATGACTTTTTGGGGTTGATAGAAGTGCCTGACTCCTATGATGGTCCTCGGCTGCAGTT TCCTCTGACGTTTACTGACATCCATATACTTCTTCAGGCCTTCAAGCAACAGCAGATACTTCATGCTCAC TACGTCTTAGAGGTGCTATTTGAAGCTAGGAAAGTCCTGAAGCAAATGCCGAATTTCTCTCATGTAAAAA CCTTTCCCGCCAAAGAGATAACAATATGTGGTGATTTGCATGGGAAACTGGATGATCTTATGCTGATTTT CTATAAGAATGGCCTGCCTTCAGAGAACAATCCATACGTTTTCAATGGTGACTTTGTAGATCGAGGAAAC AATTCCATGGAGATCCTAATGATCCTGCTTGTTTGTTTTCTTGTCTACCCCTCTGACCTGCACCTCAATA GAGGGAACCATGAAGACTTTATGATGAACCTGAGGTATGGCTTCACAAAAGAAATTTTACAAAAATACAA GCTACACGGGAGAAAAATCTTACAAGTCTTGGAAGAAGTCTATACCTGGCTCCCAATCGGTACAATTATT GACAACGAAATCCTGGTCATACATGGTGGGATATCAGAATCCACAGACTTGAATACACTCCACCAGTTAC AAAGAAATAAAATGAAATCTGTGTTGATGCCGCCAGTGTTGGGAAATCAAGAGACTGGCGAAAAGAGAAA TAAATCAGCGTCAAATTATGTTGAACCAAGGAAGGTTGAACCAGACAAGACTCCTTCTGAAGACTTGACA AAGCAAGAATGGGAACAAATAGTTGACATTCTGTGGAGTGACCCCAGAGGCAAAAAAGGCTGTTATCCAA ACACAAGCCGAGGAGGGGGCTGCTATTTTGGACCAGATGTTACCTCTAAGGTTCTTAGTAAAAACCAGTT GAAGATGCTCATTAGGTCTCATGAGTGTAAACCCGACGGGTATGAAGTCTCTCATGATGGAAAGGTCATT ACTGTATTTTCTGCTTCTAATTATTATGAAGAAGGCAGCAACCGAGGAGCTTACATCAGACTAAGTTATG GTACAATGCCACAATTTTTCCAGTACCAAGTAACTAGTACATCATGCCTGAACCCTCTTCACCAAAGGAT GAATGCTATGGAATCCAGTGCTTTCAAGATACTAAAGGAAAAAATGATTTCACGAAAAACTGATCTCATC AACGCTTTCGAGCTCCGTGACCACAGTAGGTCAGGAAGGATTTCATTGGCCGAGTGGGCATTTTCTATGG AGAACATTTTGGGGCTGAACTTACCATGGAGATCTCTAAGTTCACACCTGGTAACCATTGATTCCAGCGG AAGTGTTGACTACATGTCCAGCTTTGACGATATCCGTATTGAAAAGCCTACGAAAGATATGAAGTCCAAT CTAACTGAAACTATGTACAGATACCGATCTGACCTGAAAATCATATTTAATATCATCGACAGTGATCAAT CAGGTCTGATCTCCATGGATGAATTTCGGACCATGTGGAAACTTTTCAATGCTCATTACAAAGCTCATAT AGATGATTCCCAAATTGATGAGCTTGCCAGCATAGTGGACTTCAACAAAGATGGCAACATCGACTTTAAT GAGTTTTTAAAGGCTTTCTATGTAGTACATAAATATGATAAGCCAGGGACCAGTCTTGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219324 representing NM_011147
Red=Cloning site Green=Tags(s) MGCGTSSKKGNKSKKVIKAALVIQNWYRRYRARLRVRQHYALAIFQSIEYSDEQGQMQLSSFFSFMLENY TKTNNEDSALVTRIFDNTRRESQIKDRDDFLGLIEVPDSYDGPRLQFPLTFTDIHILLQAFKQQQILHAH YVLEVLFEARKVLKQMPNFSHVKTFPAKEITICGDLHGKLDDLMLIFYKNGLPSENNPYVFNGDFVDRGN NSMEILMILLVCFLVYPSDLHLNRGNHEDFMMNLRYGFTKEILQKYKLHGRKILQVLEEVYTWLPIGTII DNEILVIHGGISESTDLNTLHQLQRNKMKSVLMPPVLGNQETGEKRNKSASNYVEPRKVEPDKTPSEDLT KQEWEQIVDILWSDPRGKKGCYPNTSRGGGCYFGPDVTSKVLSKNQLKMLIRSHECKPDGYEVSHDGKVI TVFSASNYYEEGSNRGAYIRLSYGTMPQFFQYQVTSTSCLNPLHQRMNAMESSAFKILKEKMISRKTDLI NAFELRDHSRSGRISLAEWAFSMENILGLNLPWRSLSSHLVTIDSSGSVDYMSSFDDIRIEKPTKDMKSN LTETMYRYRSDLKIIFNIIDSDQSGLISMDEFRTMWKLFNAHYKAHIDDSQIDELASIVDFNKDGNIDFN EFLKAFYVVHKYDKPGTSLA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011147 |
| ORF Size | 1950 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011147.1, NP_035277.1 |
| RefSeq Size | 2483 bp |
| RefSeq ORF | 1953 bp |
| Locus ID | 237178 |
| UniProt ID | O35655 |
| Gene Summary | May have a role in the recovery or adaptation response of photoreceptors. May have a role in diverse sensory neurons and in development.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220125 | Ppef1 (untagged) - Mouse protein phosphatase with EF hand calcium-binding domain 1 (Ppef1), (10ug) |
CNY 8,360.00 |
|
| MR219324 | Ppef1 (Myc-DDK-tagged) - Mouse protein phosphatase with EF hand calcium-binding domain 1 (Ppef1) |
CNY 4,840.00 |
|
| MR219324L3 | Lenti ORF clone of Ppef1 (Myc-DDK-tagged) - Mouse protein phosphatase with EF hand calcium-binding domain 1 (Ppef1) |
CNY 7,600.00 |
|
| MR219324L4 | Lenti ORF clone of Ppef1 (mGFP-tagged) - Mouse protein phosphatase with EF hand calcium-binding domain 1 (Ppef1) |
CNY 7,600.00 |
