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Ankrd16 (NM_177268) Mouse Tagged ORF Clone
CAT＃: MG219138
- TrueORF®
Ankrd16 (tGFP-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16), (10ug)
ORF Plasmid: DDK
"NM_177268" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810455F06Rik; AI646698; D430029B21Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219138 representing NM_177268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTGCCTGGGGATCCGCGGCGCCTCTGCAGGCTGGTGCAAGAGGGCCGACTGCGTGACCTTCAGG AGGAACTGGCGGTAGCTAGAGGTTGCCGGGGGCCAGCCGGAGACACCCTTCTCCACTGTGCAGCACGCCA CGGACGCCAGGATATCCTAGCGTACCTAGTGGAGGCTTGGAGTATGGACATCGAGGCTACCAACCGAGAC TACAAGCGGCCTCTGCACGAAGCTGCCTCTATGGGCCACCGGGACTGCGTGCGCTACCTCCTGGGCCGAG GTGCAGTCGTGGACTCCTTGAAGAAGGCGGACTGGACTCCTCTGATGATGGCGTGCACAAGGAAGAACCT TGATGTGATCCAGGACCTTGTAGAACACGGTGCCAATCCACTCCTGAAGAACAAGGATGGCTGGAACAGT TTCCACATTGCCAGTAGAGAAGGCCACCCTGTGATCCTCCGGTACTTGCTCACTGTCTGCCCTGATGCTT GGAAAACAGAGAGCAACATTAGAAGAACCCCTTTACACACTGCAGCAATGCACGGCTGTTTGGAAGCAGT CCAGGTGCTTCTTGAAAGGTGTCACTATGAACCAGACTGTCGAGACAACTGTGGTGTCACGCCCTTCATG GATGCAATTCAGTGTGGCCATGTTAGTATAGCCAAGCTGCTCCTTGAACAGCATAAGGCTTGCTCTTCAG CTGCAGATAGCATGGGGGCCCAGGCTCTACACCGCGCAGCAGTCACTGGGCAGGATGAAGCCATACGGTT CCTGGTATGCGGTCTTGGCATCGATGTAGATGTAAGAGCAAAGTCAAGCCAGCTCACAGCACTTCACTAT GCAGCAAAGGAAGGACAGACGAATACAGTTCAAACTCTGTTGTCCTTGGGTGCCGACATCAACTCTACAG ATGAAAGAAATCGCTCAGTCCTGCATCTGGCCTGCGCAGGTCAGCATGTGGCTTGCACCAGGCTCCTCCT ACAGTCGGGACTGAAGGATTCCGAAGACCTCACAGGCACCTTGGCCCAGCAGCTCACGAGAAGCGTAGAT ATCCTTCAGGACTTTGACCATGACGTGAAATCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219138 representing NM_177268
Red=Cloning site Green=Tags(s) MALPGDPRRLCRLVQEGRLRDLQEELAVARGCRGPAGDTLLHCAARHGRQDILAYLVEAWSMDIEATNRD YKRPLHEAASMGHRDCVRYLLGRGAVVDSLKKADWTPLMMACTRKNLDVIQDLVEHGANPLLKNKDGWNS FHIASREGHPVILRYLLTVCPDAWKTESNIRRTPLHTAAMHGCLEAVQVLLERCHYEPDCRDNCGVTPFM DAIQCGHVSIAKLLLEQHKACSSAADSMGAQALHRAAVTGQDEAIRFLVCGLGIDVDVRAKSSQLTALHY AAKEGQTNTVQTLLSLGADINSTDERNRSVLHLACAGQHVACTRLLLQSGLKDSEDLTGTLAQQLTRSVD ILQDFDHDVKS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177268 |
| ORF Size | 1083 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177268.4, NP_796242.2 |
| RefSeq Size | 2356 bp |
| RefSeq ORF | 1086 bp |
| Locus ID | 320816 |
| UniProt ID | A2AS55 |
| Gene Summary | Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity (PubMed:29769718). Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides (PubMed:29769718).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214483 | Ankrd16 (untagged) - Mouse ankyrin repeat domain 16 (Ankrd16), (10ug) |
CNY 3,990.00 |
|
| MR219138 | Ankrd16 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16) |
CNY 3,656.00 |
|
| MR219138L3 | Lenti ORF clone of Ankrd16 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16) |
CNY 5,230.00 |
|
| MR219138L4 | Lenti ORF clone of Ankrd16 (mGFP-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16) |
CNY 5,230.00 |
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