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Casc3 (NM_138660) Mouse Tagged ORF Clone
CAT＃: MG218953
- TrueORF®
Casc3 (tGFP-tagged) - Mouse cancer susceptibility candidate 3 (Casc3), (10ug)
ORF Plasmid: DDK
"NM_138660" in other vectors (4)
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CNY 9,776.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Btz; Mln51 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218953 representing NM_138660
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACCGGCGGCGGCAGCGCGCTTCCCAGGACACGGAGGACGAGGAATCCGGGGCTTCGGGTTCCG ACAGCGGCTCTCCGGCGCGGGGCGGCGGCAGCTGCAGCGGGAGCGTCGGAGGCGGCGGCAGTGGCTCGCT GCCTTCTCAGCGCGGCGGCCGCGGCGGGGGCCTTCACCTGCGGCGGGTGGAGAGCGGGGGCGCCAAGAGC GCCGAGGAGTCTGAGTGTGAGAGTGAAGATGGCATGGAAGGAGATGCTGTTCTTTCCGATTATGAAAGTG CAGAAGATTCAGAAGGTGAAGAAGACTACAGTGAAGAGGAGAATTCCAAGGTGGAGCTGAAATCAGAAGC TAACGATGCTGCTGATTCCTCTGCGAAAGAAAAGGGAGAGGAAAAGCCTGAGTCTAAAGGCACGGTGACT GGGGAGAGGCAGAGCGGCGATGGGCAGGAGAGCACAGAGCCTGTGGAGAACAAAGTGGGGAAAAAAGGCC CTAAGCACTTGGATGACGATGAGGATCGGAAAAACCCAGCCTACATCCCCAGGAAAGGGCTCTTCTTTGA GCACGATCTTCGAGGACAGACTCAGGAGGAGGAAGTCCGACCCAAGGGACGGCAGCGAAAGCTATGGAAA GATGAGGGTCGCTGGGAACATGATAAGTTCCGTGAGGATGAACAGGCCCCGAAATCTCGGCAGGAGCTAA TTGCACTTTATGGTTATGACATTCGGTCTGCTCACAATCCTGATGACATCAAACCCCGAAGAATCCGGAA ACCTCGATTTGGAAGTTCCCCACAAAGAGATCCAAACTGGATTGGTGATCGATCAAGCAAATCCCATCGC CACCAGGGTCCTGGGGGCAATCTACCACCTAGGACATTTATTAACAGGAACACGGCAGGTACTGGCCGCA TGTCTGCATCCAGGAATTACTCTCGATCTGGGGGCTTCAAGGATGGCCGCACTAGTTTCAGGCCTGTGGA GGTTGCTGGGCAGCATGGTGGCCGGTCTGCTGAGACTCTTAAGCATGAAGCTAGTTACCGGTCACGGCGT CTAGAGCAGACTCCTGTGAGGGATCCATCTCCAGAGCCAGATGCTCCATTGCTTGGAAGTCCTGAGAAAG AAGAGGTGGCCTCAGAGACGCCAGCTGCTGTACCCGACATCACCCCACCAGCTCCTGACAGGCCCATTGA GAAGAAGTCCTATTCCCGGGCAAGAAGGACCAGGACCAAAGTTGGGGATGCAGTCAAAGCTGCTGAGGAG GTTCCTCCTCCATCTGAAGGGCTGGCCTCAACAGCCACAGTCCCCGAAACGACTCCAGCTGCTAAGACTG GGAACTGGGAGGCTCCAGTAGACTCTACCACAGGTGGACTTGAGCAAGATGTGGCGCAGCTAAATATAGC AGAACAAAGTTGGAGTCCAAGCCAGCCTTCATTCTTGCAGCCACGGGAACTTCGAGGTGTGCCTAACCAC ATCCACATGGGAGCAGGACCCCCACCTCAGTTTAACCGGATGGAAGAAATGGGCGTCCAGAGTGGTCGAG CTAAGCGTTACTCATCCCAGCGGCAGAGACCTGTGCCAGAGCCCCCTGCTCCTCCTGTGCATATCAGTAT CATGGAGGGACATTACTATGATCCATTGCAGTTCCAGGGACCAATCTATACCCATGGTGACAGCCCTGCC CCACTGCCCCCACAGGGCATGATCGTACAGCCGGAAATGCACCTTCCCCACCCAGGTTTACATCCCCACC AGTCACCAGGACCTCTGCCCAACCCGGGTCTCTACCCACCACCAGTGTCCATGTCCCCAGGACAGCCACC GCCCCAGCAACTGCTTGCTCCTACCTACTTTTCTGCTCCGGGTGTCATGAATTTTGGTAACCCCAATTAC CCTTATGCTCCGGGAGCATTGCCACCTCCTCCACCTCCTCATCTGTATCCTAACACGCAGGCTCCACCAC AGGTGTATGGAGGAGTGACTTACTATAACCCCGCCCAGCAGCAGGTGCAGCCCAAGCCCTCCCCACCCCG GAGGACTCCCCAGCCGGTCTCCATCAAGCCCCCCCCACCTGAGGTTGTAAGCAGGGGTTCCAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218953 representing NM_138660
Red=Cloning site Green=Tags(s) MADRRRQRASQDTEDEESGASGSDSGSPARGGGSCSGSVGGGGSGSLPSQRGGRGGGLHLRRVESGGAKS AEESECESEDGMEGDAVLSDYESAEDSEGEEDYSEEENSKVELKSEANDAADSSAKEKGEEKPESKGTVT GERQSGDGQESTEPVENKVGKKGPKHLDDDEDRKNPAYIPRKGLFFEHDLRGQTQEEEVRPKGRQRKLWK DEGRWEHDKFREDEQAPKSRQELIALYGYDIRSAHNPDDIKPRRIRKPRFGSSPQRDPNWIGDRSSKSHR HQGPGGNLPPRTFINRNTAGTGRMSASRNYSRSGGFKDGRTSFRPVEVAGQHGGRSAETLKHEASYRSRR LEQTPVRDPSPEPDAPLLGSPEKEEVASETPAAVPDITPPAPDRPIEKKSYSRARRTRTKVGDAVKAAEE VPPPSEGLASTATVPETTPAAKTGNWEAPVDSTTGGLEQDVAQLNIAEQSWSPSQPSFLQPRELRGVPNH IHMGAGPPPQFNRMEEMGVQSGRAKRYSSQRQRPVPEPPAPPVHISIMEGHYYDPLQFQGPIYTHGDSPA PLPPQGMIVQPEMHLPHPGLHPHQSPGPLPNPGLYPPPVSMSPGQPPPQQLLAPTYFSAPGVMNFGNPNY PYAPGALPPPPPPHLYPNTQAPPQVYGGVTYYNPAQQQVQPKPSPPRRTPQPVSIKPPPPEVVSRGSS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138660 |
| ORF Size | 2094 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138660.2, NP_619601.2 |
| RefSeq Size | 3764 bp |
| RefSeq ORF | 2097 bp |
| Locus ID | 192160 |
| UniProt ID | Q8K3W3 |
| Gene Summary | Required for pre-mRNA splicing as component of the spliceosome. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favoring cell recovery following stress. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220610 | Casc3 (untagged) - Mouse cancer susceptibility candidate 3 (Casc3), (10ug) |
CNY 9,030.00 |
|
| MR218953 | Casc3 (Myc-DDK-tagged) - Mouse cancer susceptibility candidate 3 (Casc3) |
CNY 8,176.00 |
|
| MR218953L3 | Lenti ORF clone of Casc3 (Myc-DDK-tagged) - Mouse cancer susceptibility candidate 3 (Casc3) |
CNY 8,840.00 |
|
| MR218953L4 | Lenti ORF clone of Casc3 (mGFP-tagged) - Mouse cancer susceptibility candidate 3 (Casc3) |
CNY 8,840.00 |
