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Nrk (NM_013724) Mouse Tagged ORF Clone
CAT＃: MG218840
- TrueORF®
Nrk (tGFP-tagged) - Mouse Nik related kinase (Nrk), (10ug)
ORF Plasmid: DDK
"NM_013724" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA407323; AA409510; AI225895; AV168563; AW538185; Nesk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218840 representing NM_013724
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGACCTGGGAGTTGGAGGGACAAGGAGGTGACAGATCTTGGCCAGTTGCCGGATCCAACTGGAA TATTCTCACTAGATAAAGCCATTGGTCTTGGTACTTATGGAAGAATCTTTTTGGGAATCCATGAAAAGAC TGGTTCATTGGTGGCTGTTAAAGTAATGAGTGCCAGGAAGACTCCTTTGCCTGAGATAGGAAGACGGGTG AGAGTGAATAAATACCAAAAGTCTGTTGGATGGAGATACAGCGATGAAGAAGAGGATCTTAGGACGGAAC TCAACCTGCTGAGGAAGTACTCTTTCCACAAAAACATTGTGACCTTCTATGGAGCATTTTTCAAGCTGAA TCCTCCTGGCCATCAGCACCAACTTTGGATGGTAATGGAGCTATGTGCAGCAGGCTCAGTCACTGATGTA GTGAGGATGACAAGAAATCAGAGTTTAAAAGAAGACTGGATCGCTTATATCTGTCGAGAAATCCTTCAGG GCTTAGCTCACCTTCATGCACACCAAGTAATTCACCGGGACATCAAAGGACAAAATGTGCTGCTGACTCA TGATGCTGAAGTCAAAATAGTGGATTTTGGAGTGAGTGCCCAAGTGAGCAGAACAAATGGACGGAGAAAT AGCTTCATTGGTACACCGTACTGGATGGCCCCAGAGGTGATTCATTGTGATGAGGACCCAAGGTGTTCCT ATGATTACAGAAGTGATGTGTGGTCTGTGGGAATCACTGCTATTGAAATGGCTGAAGGGGCTCCTCCTCT GTGTAAGCTTCAGCCTCTGGAAGCCCTTTGTGTTATTCTACGGGAAGCCGCTCCCAAAGTCAAATCCAGT GGATGGTCCCGTAAGTTCCAGAATTTCATGGAAAATTGCATGATAAAAAATTTCCTATTTCGTCCTACAT CTGGAAACATGCTTCTTCACCCATTTGTCCATGATATAAAAAATGAACGACGTGTTGTGGAGTCCTTGAC AAAACATCTTACTGGGATCATTCAAAAGAGAGAGAAAAAAGGAATACCTGTGGCCTTTGAAGGAGAAGAA GCTGCTAAGGAACAGTACATCACAAGGAGATTCAGAGGGCCTTCTTGCACTCCTGAGCTTCTCAGAGTGC CAACCAGCAGCAGATGCAGACCACTCAGAGTCCTGCATGGAGAGCCCCCTCAGCCAAGGTGGCTACCTGA TCAAGAAGACCCACAGGACCAAGAACTTCAACAACTGCAGAAGGCAGCCGGAGTGTTCATGCCTCTACAT TCTCAGGACAATACATCCAAGCTTTTCCCAAAGCAGGTTGAGGTAGCACCCTATCTCCGTGGGGCAGCTC AGGTGGTCATGCCAGTACTGGTACAGGTTGAAGCACCGCCCCAAGTAAGCAAAGCAGCCCAGATGCTCAA GTCACTCCCCACTCAGGACAACAAAGCTACTTCTCCAGAGGTCCAGGCCCCCGTAGCCGAAGGACAGCAG GCCCAGCATGAGGCCTTAGAGACAGAACAGCCTAAAGATTTGGACCAGGTGCCAGAAGAATTTCAGGGGC AAGACAGGGCACCTGAACAGCCAAGGCAGGGCCAGGCTGCTGAGCAGCAGCAGATACATAATCCGGTTCC TGAGCAGCCGCCGGAGGAGGATAGGGAGCCTGAGCAAGCTGAGGTGCAGGAAGAGGCTGTGGAGCCTCCC CAAGCTGAGATCGAGGACAAGGAACCTGAGGTAGTGCAAGTGCACGCCCAGGTGCTCCTGCCTTTACTGT CCCAGAACCGTCACGTGCTCTTGCCTCTTCATTTGGATAGACAGCTGCTCATTCCGGTAGGGGAACAAAA TGAAGAGGTACCACGAGCCCAGGCCTGGGATCTAGAGGCCTCAAGGGCAGTTGGTGCAGTGCAAGCGCTG ATCGAGGGACTATCCAGGGACTTGCTTCGAGCACCAAATGCATTTGTCACAAAACCACTTGGTCCGCTGC AGATCTTCTTGGAAAATTTGTCTACAGACGGGTTTTACACTGAGCCGGAACCGACACAGAAGAAAAAGTC GAAAGTTGCTAGTCTAAGGAAAGCAATAGCAAAACGACTAAGACCCAAGAGGTTCAGAGCAAAGGCATTA TGGCGACTTGAAGATTTTGAATTCTCAGATGTAGAAACCAGCAGGAGACGGCGCCACCGTAGATGGGAGG ACATCTTTAATCAGCATGAAGAGCAACTGAGACGAGTTGAAAATGACAGAGAAGATGACTCATCAGACAA TGATGAAGTGTTTCATTCAATTCAGGCTGAAGTCCAAATAGAACCACACGCTGCAAATCCTGCAGGAAAT GAGGTCCATGAGAGATCTGCTCCCATGCCTTGCAACCGGAATCGCACACATCGTGTGAAATTTTCTCCAA GTGTTGGTGAGGAAGAGCCTTCTTTGGAAGAAGCCCAGCCGCAGCAGCAGCAGCAGCAGCCCATGAACAT CCGACCGAGGAATTGCCTAAATCCTCAGAATTTTCAAGCTCAATCAGACTCATCTTCTGAGGAGGACAGT CCTGTGACTAGGAGGAAGTCCCAGTCATCACCGCCTTATTCTACTATTGATCAGAAGCTACTGATTGATA TCCACGTTCCAGATGGATTTAAAGTGGGGAAAATATCACCTCCTGTGTACTTGACAAATGAATGGGTAGG CTATAATGCACTCTCTGAAATCTTCTGGGATGACTGGATAATGCCTACACGTCCCGCTCGGCCACCTGAA GAAGATGGTGATTATGTTGAACTCTATGATGCTGATGCCAATGCTAATGGTGATGAAGAAGTTGCAAACG GTGCTTATGAAGATCCTCGTGATGGTGCCAATGGCCATGATGACATGAATAACCAGCTTGATCAAGCAAA TGGTTATGAAGGCCATGGTGCTGCTGGCTACAATGGTGGAGACGTTGGTGGTAATCATGGTGCTGCTTTC AATGGGCCAAGAGCAAATTATCCCAGAGCGGGAATCTTGAAAAACGGTCATAATGATGGACGTGCTCTAA ACAGGGGAGCTTTTGGAGTCTTTGGAGACAATGCAGCCAGAGCTTTCCATGGAGCTGCCGGTGAAGCAGG TGCAGCCTTTGGAAACCATGGAGCCAATAGGGGCAATGGAAGAGGTAACAGAAACAGGGAGGCCAATGGA AGGAATGAAGAGAATGGAGCATTTGGACGGGATCAACATGTATTTCCAGAGTTTGAACATGAGGAATCAG ATCGTGGAACTGAGACCTCAGATTCAATTGCCTTGGAGATCACATCCTTTGATGGTGAGCAAAACAGTGG TAGACCAGTATCATCAACAACAATGGGTTTTCCTATTGGACGCTCATCTCCTAGAGGTTCTGATTTTGGG AGTGACATCTCATATAACAGCCCAATACTACATGTGTATGAAAAGGATTTCTCATCTGAGGTCTACTGTG GTTCTTTGTGGGGAGTCAATTTGTTGCTGGGCACCCAATCTCATCTGTATCTGATGGACCGAAGTGGAAA GGCAGAAATTGTTAAACTTATAAAGCGAAGACCATTCCGGCAGATTCAAGTCGTGGAGCAGCTCAATTTG CTGATTACCATCTCTGGCAAAAAGAACAGACTTCGCGTGTATCATCTGTCTTGGCTGAGGAACAAAATTC TGAATAATGATCCAAAGAGTAAGAAAAGGCAGAAGGCGATGAGGAAGAAAGAGGAAGCTTGCAAAGCTAT CGATAAGTTGATTGGCTGTGAACACTTCAGTGTCCTTCAACATGAAGAAACAACATACATTGCGGTTGCT GTGAAGTCATCAATTCACCTCTTTGCATGGGCACCAAAATCCTTTGATGAAAACACTGCCATTAAAGTGT TTCCAACTCGTGATCTTAAGCCACTGACAGTTGACCTGGCTGTTGGTTCAGAGAAAACACTGAAGATTTT CTTCAGCTCAGCTAATGGATATCATATCATCGATGCAGAATCTGAAGTTATGTCTGAGGTGACCTTGCCA AATAATAATGTCGTCATTTTACCTGATTGCTTGGGACTTGGTGTGATGCTCTCCCTCAATGCTGAAGCTG CCTCCGAGGAAGCAAACGAACAACTCCTTAAGAAGATCCTGGACGTGTGGAAGGACATACCGTCTTCTGT GGCTTTTGAGTGTACAAAGAGAATCACTGGATGGGACCAAAAGGCTATTGAAGTGCGCTCTCTTCAGTCA ACTATTCTCGAGAATGAGCTGAAGCGCCGCTCAATCAAGAAACTGAGATTTCTCTGTGCCCGTGGTGACA AGATGTTCTTTGCTTCTACACTGAGCAATGACCACAGCCGGGTTTACCTTATGAGCCTTGGAAAACTTGA AGAGCTCCATAGAAGCTATGCTGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218840 representing NM_013724
Red=Cloning site Green=Tags(s) MAGPGSWRDKEVTDLGQLPDPTGIFSLDKAIGLGTYGRIFLGIHEKTGSLVAVKVMSARKTPLPEIGRRV RVNKYQKSVGWRYSDEEEDLRTELNLLRKYSFHKNIVTFYGAFFKLNPPGHQHQLWMVMELCAAGSVTDV VRMTRNQSLKEDWIAYICREILQGLAHLHAHQVIHRDIKGQNVLLTHDAEVKIVDFGVSAQVSRTNGRRN SFIGTPYWMAPEVIHCDEDPRCSYDYRSDVWSVGITAIEMAEGAPPLCKLQPLEALCVILREAAPKVKSS GWSRKFQNFMENCMIKNFLFRPTSGNMLLHPFVHDIKNERRVVESLTKHLTGIIQKREKKGIPVAFEGEE AAKEQYITRRFRGPSCTPELLRVPTSSRCRPLRVLHGEPPQPRWLPDQEDPQDQELQQLQKAAGVFMPLH SQDNTSKLFPKQVEVAPYLRGAAQVVMPVLVQVEAPPQVSKAAQMLKSLPTQDNKATSPEVQAPVAEGQQ AQHEALETEQPKDLDQVPEEFQGQDRAPEQPRQGQAAEQQQIHNPVPEQPPEEDREPEQAEVQEEAVEPP QAEIEDKEPEVVQVHAQVLLPLLSQNRHVLLPLHLDRQLLIPVGEQNEEVPRAQAWDLEASRAVGAVQAL IEGLSRDLLRAPNAFVTKPLGPLQIFLENLSTDGFYTEPEPTQKKKSKVASLRKAIAKRLRPKRFRAKAL WRLEDFEFSDVETSRRRRHRRWEDIFNQHEEQLRRVENDREDDSSDNDEVFHSIQAEVQIEPHAANPAGN EVHERSAPMPCNRNRTHRVKFSPSVGEEEPSLEEAQPQQQQQQPMNIRPRNCLNPQNFQAQSDSSSEEDS PVTRRKSQSSPPYSTIDQKLLIDIHVPDGFKVGKISPPVYLTNEWVGYNALSEIFWDDWIMPTRPARPPE EDGDYVELYDADANANGDEEVANGAYEDPRDGANGHDDMNNQLDQANGYEGHGAAGYNGGDVGGNHGAAF NGPRANYPRAGILKNGHNDGRALNRGAFGVFGDNAARAFHGAAGEAGAAFGNHGANRGNGRGNRNREANG RNEENGAFGRDQHVFPEFEHEESDRGTETSDSIALEITSFDGEQNSGRPVSSTTMGFPIGRSSPRGSDFG SDISYNSPILHVYEKDFSSEVYCGSLWGVNLLLGTQSHLYLMDRSGKAEIVKLIKRRPFRQIQVVEQLNL LITISGKKNRLRVYHLSWLRNKILNNDPKSKKRQKAMRKKEEACKAIDKLIGCEHFSVLQHEETTYIAVA VKSSIHLFAWAPKSFDENTAIKVFPTRDLKPLTVDLAVGSEKTLKIFFSSANGYHIIDAESEVMSEVTLP NNNVVILPDCLGLGVMLSLNAEAASEEANEQLLKKILDVWKDIPSSVAFECTKRITGWDQKAIEVRSLQS TILENELKRRSIKKLRFLCARGDKMFFASTLSNDHSRVYLMSLGKLEELHRSYAV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_013724 |
| ORF Size | 4365 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_013724.2, NP_038752.2 |
| RefSeq Size | 6604 bp |
| RefSeq ORF | 4368 bp |
| Locus ID | 27206 |
| UniProt ID | Q9R0G8 |
| Gene Summary | May phosphorylate cofilin-1 and induce actin polymerization through this process, during the late stages of embryogenesis. Involved in the TNF-alpha-induced signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224497 | Nrk (untagged) - Mouse Nik related kinase (Nrk), (10ug) |
CNY 18,720.00 |
|
| MR218840 | Nrk (Myc-DDK-tagged) - Mouse Nik related kinase (Nrk) |
CNY 9,960.00 |
|
| MR218840L3 | Lenti ORF clone of Nrk (Myc-DDK-tagged) - Mouse Nik related kinase (Nrk) |
CNY 12,730.00 |
|
| MR218840L4 | Lenti ORF clone of Nrk (mGFP-tagged) - Mouse Nik related kinase (Nrk) |
CNY 12,730.00 |
