United States
Germany
Japan
United Kingdom
China
Plce1 (NM_019588) Mouse Tagged ORF Clone
CAT＃: MG218818
- TrueORF®
Plce1 (tGFP-tagged) - Mouse phospholipase C epsilon 1 (Plce1), (10ug)
ORF Plasmid: DDK
"NM_019588" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 17,860.00
货期*
3周
规格
Product images
经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4933403A21Rik; mKIAA1516; Plce; PLCepsilon |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218818 representing NM_019588
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCGGAAGAAATGGCCGCTTCTGTCCTCATCCCTGTGACTCAAAGGAAAGTGGCTTCTGCCCAGT CGGTGGCAGAAGAGAGGAGTGTAAAGGTCTCAGATGCGGGGATTCCTCGGGCACGTGCTGGCAGGCAGGG TGCGCTCATCCCTCCTACCATCTCACAGTGGAACAAACATAAGGAAGAGTCGTCTAGAAGTGATTTGTCC AAGGTCTTCTCAATAGCGAGGGGGGAACTAGTCTGCGATGAGAATTCCAACGAAGAGGGCTGGGAGGAAA ACGCACCAGACTCCCCGGAAAACCACGCGATGAACGGCAACAGTTTAGTGCAAAGCCACCAGCACCAGTT TCCCCGGAGCCAGCTTTGTGAAGCCCGTGACTCTGTCACAGAAGACCCGTGTTTGCAGCCTGGAATTCCT TCTCCCCTGGAAAGGAAGGTGCTCCCTGGAATTCAACTGGAGATGGAGGACTCTCCCATGGACGTGAGCC CTGCGGGAAGTCAGCCTAGGATCATGGAGTCCAGCGGACCTCACTCTGACCGCAACACGGCGGTATTTCA CTTCCATTATGAAGCTGACAGGACAATGTCGGATGCTTTTCATACCCTGTCAGAAAACCTGATTTTGGAT GACTGTGCAAATTGTGTTACTCTTCCTGGGGGGCAGCAAAATAAAAATTGCATGGCGTATGCTTGCAAAC TGGTGGAGTTGACAAGAACCTGTGGAAGTAAGAATGGGCAAGTCCAGTGTGAGCACTGTACCTCGCTGCG CGATGAATACCTGTGCTTCGAAAGCTCCTGCTCAAAGGCCGATGAGGTCTGCTCAGGTGGTGGCTTTTGC GAGGACGGCTTTGCTCACGGTCCTGCTGCCAAGACTTTTCTGAGCCCTCTGGAGGATTTCTCTGACAATT GTGAAGACGTAGACGATTTTTTTAAAAGCAAAAAGGAGCGGTCCACGTTGTTAGTCCGAAGGTTTTGTAA AAATGACAGGGAAGTTAAGAAGTCTGTGTATACTGGGACCAGAGCCATCATGAGAACTCTGCCTTCTGGC TGTATTGGGCCAGCTGCTTGGAATTACGTTGATCAGAAGAAAGCTGGTCTCCTATGGCCTTGTGGGAATG TAATGGGAACTCTGTCAGCAATGGACATAAGGCAAAGTGGGAGCCAACGTCTGTCTGAAGCCCAGTGGTG CCTGATCTACAGTGCCGTGAGAAGAGGGGAAGAAATAGAAGATACGGTTGGATCTCTTCTCCATTGCTCC ACACAGCTCCCAAACTCTGAGACAGCCCATGGAAGGATTGAAGATGGTCCCTGCTTAAAGCAGTGTGTAC GAGACACTGAATGTGAATTCCGTGCTACCCTCCAAAGGACATCCATAGCTCAGTACATCACCGGTTCTCT TCTAGAGGCAACTACATCTTTAGGAGCAAGAAGTGGCCTTCTCAGTTCCTTCGGAGGATCCACTGGACGA ATAATGCTGAAAGAACGCCAACTGGGCACCTCTATGGCCAATTCCAATCCTGTTCCTTCAAGTTCAGCTG GAATCAGCAAGGAGCTGATTGATCTCCAGCCCCTCATTCAGTTCCCGGAGGAAGTAGCCAGCATCTTAAC AGAGCAGGAACAGAACATTTACCGCAGGGTCCTGCCCATGGACTACCTCTGCTTCTTAACCCGGGACTTG AGTTCACCTGAATGCCAGAGATCACTGCCCCGTCTCAAAGCATCCATCTCAGAATCCATCCTCACGTCTC AAAGTGGAGAACACAATGCCCTTGAAGACCTTGTGATGAGATTTAACGAGGTGAGCTCCTGGGTAACATG GCTGATCCTCACAGCAGGCTCCATGGAGGAGAAGCGGGAAGTCTTTTCTTATTTGGTGCATGTGGCCAAA TGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCCTGGCTGGCCTCAGGTCAAGGAAAGTTC TAAAGATGTGGCAGTTCATGGACCAGTCTGACATTGAGACCATGCGGAGCCTGAAGGATGCCATGGCCCA GCATGAGTCTTCCGTGGAGTACAAGAAGGTAGTGACCCGAGCCTTGCACATCCCCGGCTGCAAAGTGGTC CCCTTCTGTGGGGTGTTCCTGAAGGAGCTCTGTGAGGTGCTTGATGGTGCCTCTGGCCTCCTGAAGCTCT GCCCACGGTACAGCTCCCAAGAAGAAGCTCTGGAGTTTGTAGCCGATTACAGTGGACAAGATAACTTCTT ACAACGAGTGGGACAGAATGGCTTAAAGAATTCAGAGAAGGAGCTCACGGTCAACAGCATCTTTCAGGTC ATCCGGAGCTGCAGCCGAAGTTTGGAGATGGAGGAGGAAGACAGTGCCAGTGAAGGGAGCGGCTCTAGGA AAAACTCCCTGAAGGACAAAGCCCGATGGCAGTTTATAATTGGAGATTTGCTGGATTCAGAAAATGACAT CTTTGAGAAGTCCAAAGAGTGTGACCCCCATGGATCCGAGGAGTCACAGAAAGCCTTTGACCATGGGACA GAGCTTATCCCATGGTACGTGCTGTCCATCCAAGCAGATGTGCACCAGTTCCTATTGCAGGGGGCCACAG TCATCCACTATGACCAGGACACACATCTCTCGGCCCGCTGCTTTCTCCAGTTGCAGCCTGACAATAGCAC GTTGACCTGGATGAAACCTCCCACTGCCTCCCCAGCAGGCGCCAGACCCAAGCTCGGTGTGCTTAGTAAC ATGGCCGAGCCTGGCAAGTTCCCATCCCCAGGCAACGCCGGGGTGAGCGGCCTGGCAGAGGGAATTCTGG ATCTGTTCTCAGTGAAGGCTGTGTACATGGGACACCCTGGCATTGATATACACACTGTGTGTGTCCAGAA CAAACTTAGTAGCATGCTTCTATCGGAGACGGGGGTCACACTGCTCTATGGGCTTCAGACCACGGACAAT AGATTACTGCACTTTGTGGCGCCAAAGCACACGGCTGAGATGCTTTTCAGTGGATTGCTGGAACTCACTA CAGCTGTGAGAAAGATAAGGAGGTTCCCTGACCAACGACAGCAGTGGCTTCGGAAACAGTATGTCAGCCT CTATCAGGAGGATGGTCGGTACGAAGGCCCAACCTTGGCACATGCTGTGGAGTTGTTTGGTGGTAGGCGG TGGAGCACTAGAAACCCCAGCCCTGGAATGTCAGCAAAGAATGCTGAGAAGCCCAACATGCAGAGAAACA ACACCCTGGGCATAAGCACCACCAAGAAAAAGAAGAAAATGCTCATGAGGGGAGAGAGTGGAGAGGTGAC GGACGATGAGATGGCTACCCGCAAGGCCAAAATGTACAGAGAGTGTCGCAGCCGGAGCGGCTCTGATCCT CAAGACGTTAACGAACAAGAAGAGTCGGAGGCCAATGTCATCACTAACCCTCCCAACCCCCTCCATTCCA GAAGAGCCTACTCTTTGACCACGGCTGGGTCCCCCAACTTGGCTACCGGGATGTCATCTCCCATCAGTGC CTGGAGCAGCAGCAGCTGGCATGGGCGAATCCGAGGAGGAATGCAAGGCTTCCAGAGCTTCATGGTCTCA GACAGTAACATGAGTTTCGTTGAATTCGTCGAGCTGTTCAAATCATTCAGCATCAGGAGCCGCAAGGACT TGAAGGACATCTTTGACATCTACTCTGTACCCTGCAATCGGTCTGCCTCGGAGTCAGCCCCACTCTACAC CAACCTGACAATCGAAGAAAATACCAGCGATCTTCAGCCTGACCTAGATTTGCTGACTAGAAACGTCTCC GACCTGGGGCTGTTCATTAAAAGTAAACAGCAGCTTTCTGACAACCAGAGGCAGATATCTGATGCTATCG CTGCTGCAAGCATCGTGACCAATGGTACTGGGATTGAAAGCACGTCCCTGGGCATATTTGGGGTCGGCAT ACTCCAGCTCAATGACTTCCTGGTGAATTGCCAAGGAGAACACTGCACATATGATGAAATCCTGAGCATC ATCCAGAAATTTGAGCCTAGCGTCAGCATGTGTCATCAAGGCCTACTGTCATTTGAAGGGTTTGCAAGGT TTCTGATGGATAAAGACAATTTCGCCTCAAAAAATGATGAGTCACGGGAGAACAAGAAAGAACTGCAGCT ACCCCTCTCCTACTATTATATAGAATCTTCACACAACACCTACCTCACCGGCCATCAGCTCAAGGGGGAG TCCTCTGTGGAGCTCTATAGCCAGGTCCTCCTGCAAGGATGTAGAAGCATAGAGCTGGACTGCTGGGACG GGGATGATGGGATGCCCATCATCTATCATGGGCATACACTGACAACCAAGATCCCCTTCAAGGAAGTGGT GGAAGCCATCGATCGCAGTGCCTTCATCACCTCAGACCTGCCAATCATCATATCCATTGAGAACCATTGT TCATTGCCTCAACAACGAAAAATGGCAGAAATTTTCAAGAGTGTGTTTGGAGAAAAGCTGGTGGCTAAGT TCTTATTCGAGACCGATTTCTCAGATGACCCAATGCTTCCCTCACCTGATCAACTTAGAAGGAAAGTACT CCTTAAAAACAAGAAGCTAAAGGCCCATCAGACACCTGTCGACATCCTAAAACAAAAGGCTCATCAGTTA GCATCCATGCAAGCACAGGCTTTCACCGGTGGGAATGCCAACCCGCCACCTGCCAGTAATGAGGAAGAGG AAGATGAGGAGGATGAATATGATTACGATTACGAGTCCCTGTCCGATGACAATATTCTGGAAGACAGACC TGAGAACAAATCATGCGCTGACAAGCTTCAGTTTGAGTACAATGAAGAGGTCCCCAAGAGGATAAAGAAA GCAGACAACTCCTCTGGCAATAAGGGAAAGGTGTATGACATGGAACTGGGAGAGGAGTTTTACCTTCCTC AGAACAAAAAGGAAAGCAGGCAGATTGCACCGGAGCTTTCTGACCTCGTCATTTATTGCCAGGCAGTAAA ATTTCCAGGCCTGTCAACTCTAAATTCATCTGGCTCTAGCAGAGGGAAAGAAAGGAAAAGCAGGAAGTCC ATTTTTGGCAACAATCCTGGCAGAATGAGCCCCGGGGAGACAGCGCCATTTAACAGAACATCTGGAAAAG GTTCCTGTGAAGGGATGCGACACACCTGGGAGGAATCTTCTCCACTCAGCCCAAGCACATCCCTCAGCGC TATCATCAGGACTCCTAAATGCTATCACATCTCCTCACTGAACGAGAACGCCGCCAAACGTCTGTGCCGC AGGGGTTCTCAGAAACTGATCCAGCACACCGCTTATCAGCTGCTGAGGACCTACCCGGCAGCGACCAGAA TCGACTCCTCCAATCCCAACCCCATCATGTTCTGGCTCCATGGGATACAGCTTGTAGCTCTAAACTACCA AACCGATGACCTTCCTTTACATTTAAACGCCGCCATGTTTGAGGCGAATGGTGGGTGTGGTTATGTTTTG AAGCCCCCTGTCCTGTGGGATAAAAGCTGTCCCATGTACCAGAAGTTTTCTCCACTGGAAAGAGACCTAG ACAACCTGGATCCTGCTATCTATTCTTTAACTATCATCTCTGGCCAGAATGTGTGCCCTAGTAACAGCAC CGGAAGTCCATGCATTGAAGTCGACGTCTTGGGCATGCCCCTGGACAGCTGCCATTTCCGCACAAAACCC ATCCATCGAAACACTCTAAACCCCATGTGGAATGAGCAGTTTCTCTTCCGGGTTCACTTTGAAGATCTTG TATTTCTTCGTTTTGCAGTCGTGGAAAACAACAGTTCGGCGATAACGGCTCAGAGAATCATTCCACTCAG AGCTTTGAAACGAGGATATCGACATCTTCAGCTGCGGAACCTCCACAATGAAATCTTGGAAATCTCTAGC TTATTCATAAACAGCAGAAGGATGGAAGAAAACCCCTCTGGCAGTTCCATGCCAGCCTCGTTGATGTTTA ATACTGAAGAGAGGAAATGTTCTCAGACTCACAAAGTCACAGTGCATGGTGTCCCAGGTCCAGAGCCCTT TGCTGTTTTCACTATAAACGAAGGCACCAAGGCAAAGCAGCTTCTCCAACAGGTTTTGGCAGTTGACCAA GACACCAAATGTACTGCCACAGACTATTTTTTGATGGAAGAGAAGCATTTTATATCTAAGGAAAAGAATG AATGCAGGAAACAACCATTCCAGAGAGCTGTGGGTCCAGAAGAAGATATTGTGCAGATTTTAAACAGTTG GTTCCCAGAAGAAGGCTACGTAGGCAGGATTGTCTTAAAGCCCCAACAGGAAACTCTAGAAGAGAAAAGC ATTGTCTTCGACGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTGCAAGTGCATGATG TCTCTCCAGAGCAACCTCGAACTGTCATCAAAGCCCCACGTGTCAGCACTGCACAGGATGTAATTCAGCA GACCTTATGCAAAGCCAAATATTCCTACAGCATCCTGAACAACCCCAACCCATGTGACTATGTGCTTTTG GAAGAGGTGCTGAAAGATGCAGCTAATAAGAAGTCTTCTACCCCAAAGTCCTCGCAGAGGATCCTCTTGG ACCAAGAATGTGTGTTTCAAGCGCAAAGCAAGTGGAAGGGTGCGGGAAAGTTCATACTTAAGCTAAAGGA GCAGGTGCAGGCATCCCGAGAAGACAAAAGGAGGGGTATCTCCTTTGCAAGTGAACTCAAGAAGCTCACT AAGTCCACTAAGCAGTCTCGAGGGCTCCCATCACCTCCTCAGCTTGTGGCCTCAGAAAGTGTTCAAAGCA AGGAGGAGAAACCTGTGGGTGCCTTGTCCTCTAGTGACACAGTGGGTTACCAGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218818 representing NM_019588
Red=Cloning site Green=Tags(s) MTSEEMAASVLIPVTQRKVASAQSVAEERSVKVSDAGIPRARAGRQGALIPPTISQWNKHKEESSRSDLS KVFSIARGELVCDENSNEEGWEENAPDSPENHAMNGNSLVQSHQHQFPRSQLCEARDSVTEDPCLQPGIP SPLERKVLPGIQLEMEDSPMDVSPAGSQPRIMESSGPHSDRNTAVFHFHYEADRTMSDAFHTLSENLILD DCANCVTLPGGQQNKNCMAYACKLVELTRTCGSKNGQVQCEHCTSLRDEYLCFESSCSKADEVCSGGGFC EDGFAHGPAAKTFLSPLEDFSDNCEDVDDFFKSKKERSTLLVRRFCKNDREVKKSVYTGTRAIMRTLPSG CIGPAAWNYVDQKKAGLLWPCGNVMGTLSAMDIRQSGSQRLSEAQWCLIYSAVRRGEEIEDTVGSLLHCS TQLPNSETAHGRIEDGPCLKQCVRDTECEFRATLQRTSIAQYITGSLLEATTSLGARSGLLSSFGGSTGR IMLKERQLGTSMANSNPVPSSSAGISKELIDLQPLIQFPEEVASILTEQEQNIYRRVLPMDYLCFLTRDL SSPECQRSLPRLKASISESILTSQSGEHNALEDLVMRFNEVSSWVTWLILTAGSMEEKREVFSYLVHVAK CCWNMGNYNAVMEFLAGLRSRKVLKMWQFMDQSDIETMRSLKDAMAQHESSVEYKKVVTRALHIPGCKVV PFCGVFLKELCEVLDGASGLLKLCPRYSSQEEALEFVADYSGQDNFLQRVGQNGLKNSEKELTVNSIFQV IRSCSRSLEMEEEDSASEGSGSRKNSLKDKARWQFIIGDLLDSENDIFEKSKECDPHGSEESQKAFDHGT ELIPWYVLSIQADVHQFLLQGATVIHYDQDTHLSARCFLQLQPDNSTLTWMKPPTASPAGARPKLGVLSN MAEPGKFPSPGNAGVSGLAEGILDLFSVKAVYMGHPGIDIHTVCVQNKLSSMLLSETGVTLLYGLQTTDN RLLHFVAPKHTAEMLFSGLLELTTAVRKIRRFPDQRQQWLRKQYVSLYQEDGRYEGPTLAHAVELFGGRR WSTRNPSPGMSAKNAEKPNMQRNNTLGISTTKKKKKMLMRGESGEVTDDEMATRKAKMYRECRSRSGSDP QDVNEQEESEANVITNPPNPLHSRRAYSLTTAGSPNLATGMSSPISAWSSSSWHGRIRGGMQGFQSFMVS DSNMSFVEFVELFKSFSIRSRKDLKDIFDIYSVPCNRSASESAPLYTNLTIEENTSDLQPDLDLLTRNVS DLGLFIKSKQQLSDNQRQISDAIAAASIVTNGTGIESTSLGIFGVGILQLNDFLVNCQGEHCTYDEILSI IQKFEPSVSMCHQGLLSFEGFARFLMDKDNFASKNDESRENKKELQLPLSYYYIESSHNTYLTGHQLKGE SSVELYSQVLLQGCRSIELDCWDGDDGMPIIYHGHTLTTKIPFKEVVEAIDRSAFITSDLPIIISIENHC SLPQQRKMAEIFKSVFGEKLVAKFLFETDFSDDPMLPSPDQLRRKVLLKNKKLKAHQTPVDILKQKAHQL ASMQAQAFTGGNANPPPASNEEEEDEEDEYDYDYESLSDDNILEDRPENKSCADKLQFEYNEEVPKRIKK ADNSSGNKGKVYDMELGEEFYLPQNKKESRQIAPELSDLVIYCQAVKFPGLSTLNSSGSSRGKERKSRKS IFGNNPGRMSPGETAPFNRTSGKGSCEGMRHTWEESSPLSPSTSLSAIIRTPKCYHISSLNENAAKRLCR RGSQKLIQHTAYQLLRTYPAATRIDSSNPNPIMFWLHGIQLVALNYQTDDLPLHLNAAMFEANGGCGYVL KPPVLWDKSCPMYQKFSPLERDLDNLDPAIYSLTIISGQNVCPSNSTGSPCIEVDVLGMPLDSCHFRTKP IHRNTLNPMWNEQFLFRVHFEDLVFLRFAVVENNSSAITAQRIIPLRALKRGYRHLQLRNLHNEILEISS LFINSRRMEENPSGSSMPASLMFNTEERKCSQTHKVTVHGVPGPEPFAVFTINEGTKAKQLLQQVLAVDQ DTKCTATDYFLMEEKHFISKEKNECRKQPFQRAVGPEEDIVQILNSWFPEEGYVGRIVLKPQQETLEEKS IVFDDKEVILSSEEESFFVQVHDVSPEQPRTVIKAPRVSTAQDVIQQTLCKAKYSYSILNNPNPCDYVLL EEVLKDAANKKSSTPKSSQRILLDQECVFQAQSKWKGAGKFILKLKEQVQASREDKRRGISFASELKKLT KSTKQSRGLPSPPQLVASESVQSKEEKPVGALSSSDTVGYQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019588 |
| ORF Size | 6846 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019588.2, NP_062534.2 |
| RefSeq Size | 9525 bp |
| RefSeq ORF | 6849 bp |
| Locus ID | 74055 |
| UniProt ID | Q8K4S1 |
| Gene Summary | The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. PLCE1 is a bifunctional enzyme which also regulates small GTPases of the Ras superfamily through its Ras guanine-exchange factor (RasGEF) activity. As an effector of heterotrimeric and small G-protein, it may play a role in cell survival, cell growth, actin organization and T-cell activation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
