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Pask (NM_080850) Mouse Tagged ORF Clone
CAT＃: MG218778
- TrueORF®
Pask (tGFP-tagged) - Mouse PAS domain containing serine/threonine kinase (Pask), (10ug)
ORF Plasmid: DDK
"NM_080850" in other vectors (4)
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CNY 15,200.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA0135 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218778 representing NM_080850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACAGAGGTCCACCAGTTTTTGCTGAGGACTGGAAATGTTTGTCTGAGAGTCCTCCTGTCCAAG AAGGCCCAGCGGCCCAGGCTACCTTTGAGCCCAGCAAGCCACTCTCCATAGCCCACAAACACCTGAGCAG GAAGAATGGGCTTTCTAGACTCTGCCAGAGCCGAATGGCGCTCTCTGAGGACAGGTGGAGCTCTTACTGT CTTTCATCATTGGCTGCCCAAAACATCTGCACAAGCAAACTGCACTGCGCTGCTGCACCTGAGTATGCTG ACCCTACAGCTGGGCCACTGGGCAGTACTTCCTGCTGCTCCTTGCTGCGTGGCCTGGCTTCAGGATGCTC TGGGTCCCTGCTTTCGACCCCTGTGTGCAACCCCAACAAGGCTGTCTTTACCGTGGATGCCAAGACCACA GAGATTCTGGTTGCCAACGACAAAGCCTGCAGCCTCCTAGGGTATAGCAGTCATGACCTGATTGGCCAAA AGCTCGCCCAATTCTTCCTCAAATCTGATTCCGAGGTGGTGGAAGCCCTCAGCGAGGAACATGTGGAGGC TGATGGCCACGCTGCAGTGGTGTTCGGCACAGTGGTGGACATCGTCAGCCGAATTGGGGAGAAGATTCCA GTCTCTGTGTGGATAAAGAGATTGCAGCAAGATCGTGGCTTGTGCTGTGTAGTGGTTCTGGAGCCTGTGG AGAGAGTGTCAGCCTGGGTCGCCTTCCAGAGTGATGGAACTATCACATCATGCGACAGTCTCTTTGCTCA TCTCCATGGGTTCACATCTCCAAAGGATGTAGTTGGGCAGTGTGTCATAGACTTGATTCCATCCATGCAG CTCCCTCCTCCTGGCCAACATATCCCAAAGAGTCTCAAGATTCAAAGGTCTGTTGGGAGAGCCAGAGACG GCACCACCTTCCCTCTCAGTTTGAAGCTGAAATCCAAGCCCAGTGGAAGGGCTGTAGCTGACAGTGAGGC GGCCTCTGAACCAGGTTACCAGGCTTCTGTGTGGGTGTTCTGCACCATCAGTGGCCTCATCACTCTGCTG CCAGATGGCACAATTTATGGAGTCAACCACAGCTTTGCACTAATGCTGTTTGGTTATGGAAAGACAGAGC TCCTGGGCAAGAATATCACTTTCCTGATTCCTGGTTTCTACCACTACATGGACCTCACATATGATAGTTC GGTACAGCTGCCAGATCTGGTCAATTGCCTCGACATTGGCAGAAAGAGTGGGCCTGGGGAGATGAACTCT GATGCCCAACACAACTGGGAACTGGCCAGTGGGGCCCAGGGTCCAAGAATTGACGTGGTGTTGGCACGGG ACCACATGCCATCACAAGATGAGACCCTGAAGCTGGTGGGAGGACAAGTCTCCTCTAGGACTCAGACACG ATTGGAAACTGGATACAAGATACTTCCTTCTTCTGCATGTCAGCCCTCACTGGGGGTGGACAGCAACCCA GAGGATGGTGAGCAGTCATTGCTCACAGACCAGCAAAGCATTCCAAAGAGAAACCTGCCAGCCCATGGTG GTCAGAACCAGCTAGACACCTCAGAAATATCCCTACCAGTCCTTAAAGAACATTTATTGTCTGAAATTCA GAAAAATATCTCAGAAGAAAGCCCACTAACCCACAGGAAATGGTTATCCAAGGTGCAACAAAACCCCACA AAAGGAAGCCTGCCCATCCACGAGGAGCAGCTATTGTTTGCAGGCCAGCATATCCATGTCTTGGGAAAGG AGGATCCTTCAGCAGCAGAGAGCTACAGGGAGAGTCTTTTAGAAGAAAGCAAGTCCAAACCAGTGGATGC AAAACTGTTTGCTTCATGTGAAGATTCTGAGCCTCTAGTTTCAGTTAAGGACAGGGGCAGCAGTGTGGAC ACGTGTAATCTGCATCAGGAAGCTCAGTTGGAGCTGATGGGTGTCAGCAGTCCTAACCCCTGGGCTGATG CCACCATGCCTGAGCCCCACACCACAGGGCAGATAGCAGGCGGGAGCCTTACATATTGCCCACAGTACAG AAGCGAGTGGGCCTCACAGCAGCGAGGCCAGGACTCGGCTCCCAGCCCCTCTGGAATGGCGTGTGTCTTG CTTGGGACACCCACTCTGGATGAACCATGGCCAGGAGTGAGAAATGACAGAGAAGAGCTGCAGACTTGCT TGATTAAGGAACAGTTATCCAAGTCAAGCTGTGAGGGAAATCTGGGCATCTCCCGTGTGGAACTCGTTCC TGAAGAACATCCTCCTTTCACCGCCCCCGTGTCTTTCTGTGATCTGGGAGGCCGAGATCTACACGCTAGC CGCTCAGGCAGCTCCTCAGCCTGCTATGCTTTGGCCACCTACCTCCCTGGTGTCCTGGAGGCAGTGGAAG CTCAGGAGGCTGATGTGAATTCTTATTCCTGGAACCTCAAGGAGCTCTTTCTCAAGGACCAGACAGACAG AACTCCATCACATTGTTCCTGTACCACATCTGAACTCAGCGAGGTGCCATCTCTTTCAGTGGTGGGGTCA GATTTAGATGTGGGTATTCTGCATAGGCAGACTTCAGATATTTTAGTTGACCGAGAGATGTTGCTCCTTA CTGGGACCTATTTTGACCTTAGTGAAGGCCAACGGTTCCAGGAGATGGGAGCAGGACATGATCGAGCAGA ACTGTCTAACATTTCTTTGGTATCCTCTGAACACTATGAAACTAGTGACATAGAAAGCCCAGGTTGTGAC CCTCCCTTACCAGATCCTGGTCCCAACGATATGTGCCTATCAGCAGAGAAGCCCAGGCCGAGTGCCCAGA TCACTTCCACTCCTGTGGCACGTGGGGCCACCAGCCTGCAGCAGGAGATCCAAGAGGGCATCTACTCTGG AAGCTGCTACCACCGGGATGGCTTACAACTGAGTATACAGTTTGAAGTCAAGCGTGTGGAGCTCCAGGGC TCTGCGACCCTGTTCTGCTGCTGGCTGGTGAAGGACCTCTTCCATAGCCACCGGGACTCAGCCACACGAA CCCGCCTGTTCCTTGCCAGTCTGCCTAGCTCTACCCACTCCATGCCTGAGCTGTCTGGATCCAGCTTTGG AGAGGTGCTCAGAGCCAAGCCTTGGTTTGAGGAATCCCCAACACCTGCAGAGCTAGAGGGGCTGGCGGCC TGTGAGGGCGAGTACGACTATAAGTATAACACCATCAGCCCACTTGGCAGTGGGGCCTTTGGCTTTGTGT GGACTGCAGTGGAGAAGGAATGCAACAAAGAGGTGGTGGTGAAGTTTATTAAGAAGGAGAAGGTTTTAGA GGATTGCTGGATTGAGGATCCCAAACTTGGAAGAGTTACTTTAGAGATTGCAATTCTGTCCAAGGTGGAC CATGCCAACATAATCAAGGTTCTGGATATATTCGAAAACCAAGAGTTCTTCCAGCTGGTGATGGAAAAGC ATGGCTCTGGCATGGACCTCTTTGCTTTCATTGACCACCATCCCTGCTTGGATGAGCCCCTGGCGAGTTT CATCTTTCGACAACTAGTGTCTGCTGTAGGATACCTGCACTCCCAAGGCATCATCCATAGAGACATCAAG GATGAGAACATTGTGATTGCTGAGGACTTCACAATTAAGCTGATAGATTTTGGCTCAGCTGCCTACTTAG AGAGGGGCAAACTATTTTATACCTTTTGTGGAACAATCGAATACTGTGCACCTGAGGTTCTCATTGGAAA TCCCTACAGAGGGCCAGAGCTGGAGATGTGGTCTCTGGGGGTCACCCTGTACACGCTCATCTTCGAGGAG AATCCCTTCTGTGAGGTGGAGGAGACCATGGAGGCAGTTATTCATCCCCCATTCCTGGTTTCCCAAGAAC TTATGAGTCTTCTGTCTGGACTGCTGCAGCCTTGCCCTGAGCAGCGGACCACTTTGGAGAAGCTGATCAG GGACCCCTGGGTGACACAGCCTGTGAACCTTGCTAGCTATACTTGGGAAGAGGTGTGTAGGACCAACCAG CCAGAAAGTGGCCTGCTGTCAGCTGCAAGTCTGGAGATTGGGAGTAGGAGTCCAAGTGAAATGGCTCAGA GAGAGGGTCTCTGTGGGCCTCCTGCTCCCAGGGAGACTCGTGGTGACCAGCACTGCTTGCATCTTAAGGA CCCCTCTTTGCCAGTCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218778 representing NM_080850
Red=Cloning site Green=Tags(s) MEDRGPPVFAEDWKCLSESPPVQEGPAAQATFEPSKPLSIAHKHLSRKNGLSRLCQSRMALSEDRWSSYC LSSLAAQNICTSKLHCAAAPEYADPTAGPLGSTSCCSLLRGLASGCSGSLLSTPVCNPNKAVFTVDAKTT EILVANDKACSLLGYSSHDLIGQKLAQFFLKSDSEVVEALSEEHVEADGHAAVVFGTVVDIVSRIGEKIP VSVWIKRLQQDRGLCCVVVLEPVERVSAWVAFQSDGTITSCDSLFAHLHGFTSPKDVVGQCVIDLIPSMQ LPPPGQHIPKSLKIQRSVGRARDGTTFPLSLKLKSKPSGRAVADSEAASEPGYQASVWVFCTISGLITLL PDGTIYGVNHSFALMLFGYGKTELLGKNITFLIPGFYHYMDLTYDSSVQLPDLVNCLDIGRKSGPGEMNS DAQHNWELASGAQGPRIDVVLARDHMPSQDETLKLVGGQVSSRTQTRLETGYKILPSSACQPSLGVDSNP EDGEQSLLTDQQSIPKRNLPAHGGQNQLDTSEISLPVLKEHLLSEIQKNISEESPLTHRKWLSKVQQNPT KGSLPIHEEQLLFAGQHIHVLGKEDPSAAESYRESLLEESKSKPVDAKLFASCEDSEPLVSVKDRGSSVD TCNLHQEAQLELMGVSSPNPWADATMPEPHTTGQIAGGSLTYCPQYRSEWASQQRGQDSAPSPSGMACVL LGTPTLDEPWPGVRNDREELQTCLIKEQLSKSSCEGNLGISRVELVPEEHPPFTAPVSFCDLGGRDLHAS RSGSSSACYALATYLPGVLEAVEAQEADVNSYSWNLKELFLKDQTDRTPSHCSCTTSELSEVPSLSVVGS DLDVGILHRQTSDILVDREMLLLTGTYFDLSEGQRFQEMGAGHDRAELSNISLVSSEHYETSDIESPGCD PPLPDPGPNDMCLSAEKPRPSAQITSTPVARGATSLQQEIQEGIYSGSCYHRDGLQLSIQFEVKRVELQG SATLFCCWLVKDLFHSHRDSATRTRLFLASLPSSTHSMPELSGSSFGEVLRAKPWFEESPTPAELEGLAA CEGEYDYKYNTISPLGSGAFGFVWTAVEKECNKEVVVKFIKKEKVLEDCWIEDPKLGRVTLEIAILSKVD HANIIKVLDIFENQEFFQLVMEKHGSGMDLFAFIDHHPCLDEPLASFIFRQLVSAVGYLHSQGIIHRDIK DENIVIAEDFTIKLIDFGSAAYLERGKLFYTFCGTIEYCAPEVLIGNPYRGPELEMWSLGVTLYTLIFEE NPFCEVEETMEAVIHPPFLVSQELMSLLSGLLQPCPEQRTTLEKLIRDPWVTQPVNLASYTWEEVCRTNQ PESGLLSAASLEIGSRSPSEMAQREGLCGPPAPRETRGDQHCLHLKDPSLPVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080850 |
| ORF Size | 4149 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080850.2, NP_543126.2 |
| RefSeq Size | 5119 bp |
| RefSeq ORF | 4152 bp |
| Locus ID | 269224 |
| UniProt ID | Q8CEE6 |
| Gene Summary | Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224374 | Pask (untagged) - Mouse PAS domain containing serine/threonine kinase (Pask), (10ug) |
CNY 14,200.00 |
|
| MR218778 | Pask (Myc-DDK-tagged) - Mouse PAS domain containing serine/threonine kinase (Pask) |
CNY 14,192.00 |
|
| MR218778L3 | Lenti ORF clone of Pask (Myc-DDK-tagged) - Mouse PAS domain containing serine/threonine kinase (Pask) |
CNY 15,680.00 |
|
| MR218778L4 | Lenti ORF clone of Pask (mGFP-tagged) - Mouse PAS domain containing serine/threonine kinase (Pask) |
CNY 15,680.00 |
