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Lactb (NM_030717) Mouse Tagged ORF Clone
CAT＃: MG218523
- TrueORF®
Lactb (tGFP-tagged) - Mouse lactamase beta (Lactb) nuclear gene encoding mitochondrial protein, (10ug)
ORF Plasmid: DDK
"NM_030717" in other vectors (4)
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CNY 5,230.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | LACT-1; Lact1; Mrpl56 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218523 representing NM_030717
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGGCTCCTGTCAAGCGTGACAGCTCGGGCTGCGGCCACCGCAGGCCCAGCCTGGGACGGAGGGC GGCGCGGGGCGCACAGGCGACCGGGCCTGCCTGTGCTGGGCCTTGGTTGGGCCGGCGGCCTGGGGCTCGG GCTGGGGCTGGCTCTCGGCGCGAAGCTGGTGGTCGGGCTGCGGGGCGCCGTCCCCATTCAGTCCCCCGCG GACCCCGAGGCGTCCGGCACTACCGAGTTATCGCACGAGCAGGCCCTGAGCCTGGGGAGCCCGCACACGC CTGCGCCGCCAGCAGCCAGGGGCTTCTCCAGAGCCATCGAGAGCAGCGGCGATCTGCTACACCGGATCAA GGATGAGGTTGGTGCCCCCGGCATCGTGGTTGGAGTTTCTGTAGATGGAAAAGAAGTCTGGTCAGAAGGT TTAGGCTATGCAGACGTGGAGAACCGAGTACCCTGTAAACCAGAAACGGTCATGAGAATCGCAAGCATCA GCAAAAGCCTCACCATGGTGGCTCTGGCTAAACTGTGGGAAGCAGGGAAGCTGGATCTGGACCTTCCTGT GCAGCACTATGTTCCCGAGTTCCCAGAAAAAGAATACGAGGGTGAAAAGGTTTCTGTCACAACAAGATTA CTAATTTCACATTTAAGTGGAATTCGTCATTATGAAAAGGACATAAAGAAAGTGAAAGAAGAGAAAGCTT ATAAAGCCCTGAAGATGGTGAAAGGGACCCCGCCACCGCCTGACCAAGAAAAAGAACTGAAAGAAAAGGG AGGCAAAAACAACGAAAAGAGCGACACACCGAAAGCCAAAGCCGAGCAGGACAGCGAAGCCAGATGCCGC AGCGCGAAGCCAGGCAAGAAAAAGAATGACTTCGAACAAGGCGAATTGTATTTGAAAGAAAAGTTTGAAA ATTCAATTGAATCACTAAGATTATTTAAAAATGACCCTTTATTCTTTAAACCTGGTAGTCAGTTTTTGTA TTCAACGTTTGGCTATACTCTGCTGGCAGCCATAGTAGAAAGAGCTTCAGGATATAAATATTTGGATTAT ATGCAGAAAATTTTCCATGATTTGGACATGCTGACAACTGTCCAGGAGGAAAACGAGCCAGTGATTTACA ACAGAGCAAGATTTTACGTGTACAATAAAAAGAAACGTCTTGTCAACACACCTTACGTGGATAACTCCTA TAAATGGGCTGGTGGTGGATTTCTGTCCACAGTGGGTGACCTCCTGAAATTTGGAAACGCAATGCTGTAT GGCTACCAAGTTGGGCAGTTTAAGAACTCAAATGAAAATCTCTTGCCTGGATATCTCAAGCCAGAAACGA TGGTGATGATGTGGACCCCAGTCCCTAACACAGAGATGTCCTGGGATAAAGAGGGGAAATATGCAATGGC GTGGGGTGTGGTAGAGAAGAAGCAAACGTACGGATCCTGCAGGAAGCAGCGGCACTACGCCTCACATACT GGAGGTGCTGTGGGTGCCAGTAGTGTCCTGCTGGTCCTACCTGAAGAACTGGACTCAGAGGCCGTAAATA ACAAGGTTCCCCCACGAGGAATAATCGTCTCTATCATATGCAACATGCAGTCTGTGGGGCTCAATAGCAC TGCTTTGAAGATCGCTCTGGAATTTGATAAAGACAGAGCTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218523 representing NM_030717
Red=Cloning site Green=Tags(s) MYRLLSSVTARAAATAGPAWDGGRRGAHRRPGLPVLGLGWAGGLGLGLGLALGAKLVVGLRGAVPIQSPA DPEASGTTELSHEQALSLGSPHTPAPPAARGFSRAIESSGDLLHRIKDEVGAPGIVVGVSVDGKEVWSEG LGYADVENRVPCKPETVMRIASISKSLTMVALAKLWEAGKLDLDLPVQHYVPEFPEKEYEGEKVSVTTRL LISHLSGIRHYEKDIKKVKEEKAYKALKMVKGTPPPPDQEKELKEKGGKNNEKSDTPKAKAEQDSEARCR SAKPGKKKNDFEQGELYLKEKFENSIESLRLFKNDPLFFKPGSQFLYSTFGYTLLAAIVERASGYKYLDY MQKIFHDLDMLTTVQEENEPVIYNRARFYVYNKKKRLVNTPYVDNSYKWAGGGFLSTVGDLLKFGNAMLY GYQVGQFKNSNENLLPGYLKPETMVMMWTPVPNTEMSWDKEGKYAMAWGVVEKKQTYGSCRKQRHYASHT GGAVGASSVLLVLPEELDSEAVNNKVPPRGIIVSIICNMQSVGLNSTALKIALEFDKDRAD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030717 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030717.1, NP_109642.1 |
| RefSeq Size | 2031 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 80907 |
| UniProt ID | Q9EP89 |
| Gene Summary | Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (By similarity). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (By similarity). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (By similarity). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of cancer cells: probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed:28329758).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218857 | Lactb (untagged) - Mouse lactamase, beta (Lactb), nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,030.00 |
|
| MR218523 | Lactb (Myc-DDK-tagged) - Mouse lactamase, beta (Lactb), nuclear gene encoding mitochondrial protein |
CNY 4,104.00 |
|
| MR218523L3 | Lenti ORF clone of Lactb (Myc-DDK-tagged) - Mouse lactamase, beta (Lactb), nuclear gene encoding mitochondrial protein |
CNY 6,650.00 |
|
| MR218523L4 | Lenti ORF clone of Lactb (mGFP-tagged) - Mouse lactamase, beta (Lactb), nuclear gene encoding mitochondrial protein |
CNY 6,650.00 |
