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Vps41 (NM_172120) Mouse Tagged ORF Clone
CAT＃: MG218234
- TrueORF®
Vps41 (tGFP-tagged) - Mouse vacuolar protein sorting 41 (yeast) (Vps41), (10ug)
ORF Plasmid: DDK
"NM_172120" in other vectors (4)
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CNY 8,170.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI317346; mVam2; Vam2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218234 representing NM_172120
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGCAGAGGAGCAGGAAACCGAGTCTCTTGAAGAATCTACGGATGAGTCAGAGGAAGAAAGTG AAGAGGAACCCAAACTGAAGTATGAAAGGCTTTCCAATGGGGTGACAGAGATTCTTCAGAAGGATGCAGC AAGCTGTATGACGGTCCATGACAAGTTTTTGGCACTGGGAACACATTATGGCAAGGTTTATTTACTTGAT GTCCAGGGAAACATCACTCAGAAGTTTGATGTAAGTCCTGTGAAGATAAACCAAATTAGCTTGGATGACA GTGGAGAACACATGGGAGTGTGTTCAGAGGATGGCAAGCTGCAGGTCTTTGGGCTGTATTCTGGAGAAGA ATTTCACGAGACTTTTGACTGTCCCATTAAAATCATTGCTGTGCACCCACAGTTCGTGAGATCCAGTTGT AAGCAGTTTGTGACTGGCGGGAAAAAGCTGCTGCTGTTTGAACGGACCTGGATGAACAGATGGAAGTCTT CTGTGCTACACGAAGGGGAAGGAAACATAAGGAGTGTGAAGTGGAGAGGCCATCTGATTGCTTGGGCTAA CAATATGGGTGTGAAGGTTTTTGACATCACCTCAAAGCAAAGAATCAGCAATGTGCCTCGAGATGACATA AGTCTTCGCCCAGACATGTACCCCTGCAGCCTCTGCTGGAAGGACAATGTGACACTCATTATTGGCTGGG GAACTTCCATCAAGATATGCTCAGTGAAGGAACGCCACGCCAGTGAGATGAGGGATCTGCCAAGCCGATA TGTTGAGATAGTGTCTCAGTTTGAAACTGAATTCTACATTAGTGGACTTGCACCTCTCTGTGATCAGCTT GTTGTACTTTCCTACGTAAAGGAGGTTTCAGAAAAAACGGAAAGAGAATACTGTGCAAGACCTAGACTGG ACATCATCCAGCCACTTCCGGAGACTTGTGAAGAGATCTCCTCCGATGCTTTGACAGTCAGAGGCTTTCA GGAGAATGAATGTAGGGATTACCACTTAGAATACTCCGAGGGGGAATCACTCTTCTATGTCGTGAGTCCA AGAGATGTCGTCGTAGCCAAGGAACGAGACCAGGATGATCACATCGATTGGCTCCTTGAGAAGAAGAAAT ATGAAGAAGCTTTGATGGCTGCTGAAATTAGCCAAAGGAACATTAAACGACATAAGATTCTGGATATTGG GTTGGCATATGTAAACCATCTGGTGGAGAGAGGAGAGTATGACATGGCTGCAAGAAAATGCCAGAAAATT CTTGGGAAAAATGCATCCCTCTGGGAATACGAAGTTTACAAATTTAAAGAAATTGGGCAGCTTAAAGCAA TTAGTCCCTATTTACCTCGAGGGGACCCAGTTCTGAAACCACTCATCTATGAAATGATTTTGCACGAATT TCTGGAAAGTGACTATGAGGGCTTTGCCACATTGATCAGAGAGTGGCCTGGAGATCTATATAACAATTCC GTGATAGTTCAAGCAGTTCGAGACCACCTGAAGAAGGACAGTCAGAACAAAACGTTATTGAAAACACTGG CAGAATTGTACACCTATGACAAAAATTATGGCAATGCTCTGGAAATATACTTAACGTTAAGACATAAAGA TGTTTTCCAACTGATACACAAGCATAATCTTTTCAGCTCCATCAAGGATAAAATTGTGTTATTAATGGAT TTTGATTCAGAGAAAGCTGTTGACATGCTTTTGGACAATGAAGATAAAATTTCAATTAAAAAAGTGGTAG AAGAATTAGAAGACAGACCAGAACTGCAGCATGTGTATTTGCACAAGCTCTTCAAGAGAGACCATCATAA GGGACAGCGCTACCATGAAAAGCAGATCAGTCTGTATGCCGAGTATGACCGACCAAACCTGCTTCCCTTT CTCCGAGACAGTACCCATTGCCCACTTGAAAAGGCACTTGAGATCTGTCAACAAAGAAACTTTGTAGAAG AGACAGTTTACCTTCTGAGCCGAATGGGAAACAGTAGAAGTGCCCTCAAGATGATTATGGAGGAATTGCA TGATGTTGATAAAGCAATTGAGTTTGCCAAGGAACAAGATGATGGAGAGCTCTGGGAAGATCTGATTCTA TACTCCATCGACAAACCACCATTTATCACTGGCTTGTTGAACAACATTGGCACACATGTGGACCCGATTC TACTTATCCACCGAATTAAGGAAGGAATGGAGATTCCCAATTTGAGAGATTCCTTGGTTAAAATTCTGCA AGATTATAACTTGCAGATTCTGCTTCGGGAAGGCTGCAAGAAAATCCTCGTAGCTGACTCATTGTCGTTG CTGAAAAAAATGCACCGAACCCAAATGAAAGGCGTTCTGGTTGATGAAGAGAATATCTGTGAATCATGCC TTTCTCCCATCCTCCCAACAGATGCAGCGAAGCCCTTCAGCGTGGTGGTCTTCCACTGCCGGCACATGTT CCACAAGGAGTGCCTGCCTATGCCGAGCATGAATGCTCCTGCACAATACTGCAACATCTGTAGTGCTAAG AACCGTGGGCCTGGAAGTGCCATCCTGGAGATGAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218234 representing NM_172120
Red=Cloning site Green=Tags(s) MAEAEEQETESLEESTDESEEESEEEPKLKYERLSNGVTEILQKDAASCMTVHDKFLALGTHYGKVYLLD VQGNITQKFDVSPVKINQISLDDSGEHMGVCSEDGKLQVFGLYSGEEFHETFDCPIKIIAVHPQFVRSSC KQFVTGGKKLLLFERTWMNRWKSSVLHEGEGNIRSVKWRGHLIAWANNMGVKVFDITSKQRISNVPRDDI SLRPDMYPCSLCWKDNVTLIIGWGTSIKICSVKERHASEMRDLPSRYVEIVSQFETEFYISGLAPLCDQL VVLSYVKEVSEKTEREYCARPRLDIIQPLPETCEEISSDALTVRGFQENECRDYHLEYSEGESLFYVVSP RDVVVAKERDQDDHIDWLLEKKKYEEALMAAEISQRNIKRHKILDIGLAYVNHLVERGEYDMAARKCQKI LGKNASLWEYEVYKFKEIGQLKAISPYLPRGDPVLKPLIYEMILHEFLESDYEGFATLIREWPGDLYNNS VIVQAVRDHLKKDSQNKTLLKTLAELYTYDKNYGNALEIYLTLRHKDVFQLIHKHNLFSSIKDKIVLLMD FDSEKAVDMLLDNEDKISIKKVVEELEDRPELQHVYLHKLFKRDHHKGQRYHEKQISLYAEYDRPNLLPF LRDSTHCPLEKALEICQQRNFVEETVYLLSRMGNSRSALKMIMEELHDVDKAIEFAKEQDDGELWEDLIL YSIDKPPFITGLLNNIGTHVDPILLIHRIKEGMEIPNLRDSLVKILQDYNLQILLREGCKKILVADSLSL LKKMHRTQMKGVLVDEENICESCLSPILPTDAAKPFSVVVFHCRHMFHKECLPMPSMNAPAQYCNICSAK NRGPGSAILEMKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172120 |
| ORF Size | 2559 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172120.4, NP_742118.3 |
| RefSeq Size | 3212 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 218035 |
| UniProt ID | Q5KU39 |
| Gene Summary | Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act in part as a core component of the putative HOPS endosomal tethering complex is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. Involved in homotypic vesicle fusions between late endosomes and in heterotypic fusions between late endosomes and lysosomes implicated in degradation of endocytosed cargo. Required for fusion of autophagosomes with lysosomes. May link the HOPS complex to endosomal Rab7 via its association with RILP and to lysosomal membranes via its association with ARL8B, suggesting that these interactions may bring the compartments to close proximity for fusion. Involved in the direct trans-Golgi network to late endosomes transport of lysosomal membrane proteins independently of HOPS. Involved in sorting to the regulated secretory pathway presumably implicating the AP-3 adaptor complex. May play a role in HOPS-independent function in the regulated secretory pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222075 | Vps41 (untagged) - Mouse vacuolar protein sorting 41 (yeast) (Vps41), (10ug) |
CNY 10,830.00 |
|
| MR218234 | Vps41 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 41 (yeast) (Vps41) |
CNY 6,248.00 |
|
| MR218234L3 | Lenti ORF clone of Vps41 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 41 (yeast) (Vps41) |
CNY 9,310.00 |
|
| MR218234L4 | Lenti ORF clone of Vps41 (mGFP-tagged) - Mouse vacuolar protein sorting 41 (yeast) (Vps41) |
CNY 9,310.00 |
