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D130043K22Rik (NM_001081051) Mouse Tagged ORF Clone
CAT＃: MG218150
- TrueORF®
D130043K22Rik (tGFP-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik), (10ug)
ORF Plasmid: DDK
"NM_001081051" in other vectors (4)
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CNY 10,450.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930451E12Rik; Kiaa0319 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218150 representing NM_001081051
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCCCCACCAGGAGTACTGTCTTCATTGCTGCTGCTGGCAGCCATGGCAGGAGGCAGTTCTCAGC AGTGCTCTGAGGGCAGGACTTACTCCGATGCCATCATTTCACCTAACCCGGAAACCATCAGAATCATGCG GGTGTCTCAAACCTTCTCCGTGGGAGACTGCACGGCCGCTTGCTGTGACTTGCTCACCTGTGACCTGGCC TGGTGGTTTGAGGGCAGCTGCTATCTGGTGAAATGCATGCGCTCGGAGAATTGCGAGCCCAGGACCACAG GCCCCATCCGGTCTTACCTCACTTTCGTGCGCAGACCTGTCCAGAGGCCCGGGCAGCTGCTGGACTATGG AGACATGATGCTGAGCAGGGGCTCCCCCTCAGGAGCTTGGGGAGACTCCCTTGAGGACCTCAGGAAGGAC TTGCCCTTTCTTGGCAAAGACGGGGGACCAGAGGAGACCACTGAGTACTCAGATGAATACAAAGACCTGG AGCGGGGCCTCCTGCAGCCCTCCAACCAGCAAGACCCTAGAGGTAGCGCAGAGTACCCCGACTGGAGCCT GCTGCCCAGCAACGAGGGAGGTTTCAATGCTACAGCTACAGGAGACAACTCAGCTGCTTCCATGGAGAAG CTGCAGGATCCCACGCCCCACCCACTGGACCAGGAGCAGCTGCAGGCCCTGAATGAGTCAACTTGGTCCC CTACACCCGGGCACTCTTCAATAAGCAGTGTGTGGCCTTCCTCTGCGAGCCCATTACCTACAGAGGAGGG ACTGGAAGGAGAAGAGACTTTACAGCTCCAAGAACAACCGAGCAATAGCTCTGGAAAAGAGGTTCCAATG CCTTCCCATAATCCTTCCCCTGCCAGCCTGGAGTCTAGCCCAGCCACGACGGAAAAAAACTCCAACTTTA CAGTCACGCCACGGAGCAGAAAACACAGCACCCCAACTTTTCCTACCAGTACAGTCCTCACTGGGCTAAC CCCTCCTCCGTGGCCCTTGTCACCTACTGCTTCCAGGACAGTAAAGGCGCTTGCTGTGTCTGCTGGAGAT AACCTAGTACTAACCTTACCGGACAGAGAAGCAGAACTGAAGGCCTCTGTTGAACCAGCGCCCCCTGCAG ATACAACTTACTCCTATGAATGGAGTTTAATGAGCCACCCAGTAGACTTCCAAGGTAAAATCAAACAAGA AAACAAGCCGACTCTTCACCTCTCTCAATTATCTGTGGGACTCTATGCCTTCAGAGTGGCTGTTTCTAGT GAGAATGCATTTGGAGAAGGCTATGTCAATGTCACGGTTATGCCAGCTGCAAGAGTCAACCAGCCACCTG TAGCTGTTGTTTCTCCCCAGACACAAGAGCTCAGTGTGCCTTTGACCTCAGCCCTGATCGATGGCAGCCA AAGTACAGATGATACTGAAATAGTGAGTTACCACTGGGAAGAAGTCGACGGGCCCTTCCTAGGAGAGGAG TTTCCAGCTGACACCCCAATCTTACGATTGTCGAACCTAGTTCCTGGTAACTACACCTTTAGGTTGACCA TCACAGACTCGGATGGCGCTACCAACTCCACCACTGCATCCCTCGTCATCCGTGATGCCGTGGACTACCC ACCCGTTGCCAATGCAGGGCCAAATCAGACCATCACTTTGCCCCAAAACACCATCATTCTGAATGGGAAC CAGAGCAGTGATGATCACCAGATTGTTCTCTATGAGTGGTTCGCAGGCCCTGGCGGTGAGAGCAAAGAGA TGGTCATGCAGGGAGCGCAGACCCCATACCTTCACTTGTCTGAGCTGCAGGAAGGAGAGTACACATTTCA GCTGATGGTGACGGATTCCTCGGGACAGCAATCCACCGCTTTGGTCGCTGTGACTGTCCAGGCTGAGAAC AACCAGGCTCCGGTGGCCGTGGCAGGCCCCGACAAGGAGCTGGTTTTCCCGGTGCAGAGTGCTACACTGG ATGGGAGCCGGAGCAGTGACGATCACGGCATTGTCTGCTATCACTGGGAGCACATCAGAGGCCCCAGTGC AGTGGAGATGGAGAATGTGGACAAAGCCATAGCCACTGTCACCGGCCTTCAGGTGGGTATCTACCACTTC CGGTTAACCGTGAGAGACCAGCAGGGTCTGAGCAGCACGTCCACCCTCACTGTGGCTGTGAAGAAGGAAA ATAACAGCCCTCCCAGAGCCCAGGCTGGTGGCAGACATGTTCTTATACTTCCCAATAATTCCATTACTTT GGATGGTTCAAGGTCTACTGATGACCGAGGAATTGTGTCCTATCTGTGGATCCGGGATGGCCAGAGTCCA GCTGCCGGAGATGTCATTGGAGGCTCTGACCACAGGGCAGCTCTGCAGCTCACCAATCTGGTGGAAGGCG TCTACACTTTTCACTTGCTAGTCACTGACAGTCAGGGGGCCTCGGACTCAGACGCGGCCACTGTGGAGGT GCTGCCAGACCCCAAGAAGGATGGCCTGGTGGAGTTGATCCTGCAGGTTGGTGTTGAGCAGCTGACAGAG CAGCAGAAGGAGACTCTAGTGAGGCAGCTGGCTGTGCTCCTGAACGTGCTAGACTCAGACGTGAAGGTGT TGAAGATCCAGGCTCACACGGATGTCAGCACTGTGATTGTATTTTATGTACAGAGTGGATCACCTTTCAA GGTTCTCAGAGCTGCTGCTGTGGCCCGGAATCTGCACAAGCGGCTTTCCAAGGAGAAGGAGGCTTTCCTG CTTTTCAAGGTCTTGAGGGTAGACACAGCAGGGTGCCTTCTCAAGTGTTCAGGCCACGGTCACTGTGACC CCATCACGAAGCGCTGCATCTGCTCCCAGCTGTGGATGGAGAACCTCATACAGCGGTACATGTGGGATGG AGAGAGCAACTGTGAGTGGAGTGTATTCTATGTGGCAGCTCTGGCATTGACACTAACACTGCTAACTGGA GCTGTGTCTTGGCTGTGCATCTGCTGCTGCAGGAGACGGAAAAGGACTAAGATCCGGAAGAAAACCAAGT ATACCATCCTAGACAGCATGGACGAACAGGAAAGAATGGAACTGAGGCCCAAGTACGGTATCAAGCACAG GAGCACGGAGCACAACTCCAGTCTGATGGTGTCGGAGTCTGAGTTTGAAAGCGACCAGGACACGCTATTC AGCCGAGAGAGGATGGAGAGAGGAGTCCTGAAGGGTTCCCTGAATGGCTGTGCCAGAAATGGAGTTTCCT TCGGTTACTACTCAAAGGACAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218150 representing NM_001081051
Red=Cloning site Green=Tags(s) MVSPPGVLSSLLLLAAMAGGSSQQCSEGRTYSDAIISPNPETIRIMRVSQTFSVGDCTAACCDLLTCDLA WWFEGSCYLVKCMRSENCEPRTTGPIRSYLTFVRRPVQRPGQLLDYGDMMLSRGSPSGAWGDSLEDLRKD LPFLGKDGGPEETTEYSDEYKDLERGLLQPSNQQDPRGSAEYPDWSLLPSNEGGFNATATGDNSAASMEK LQDPTPHPLDQEQLQALNESTWSPTPGHSSISSVWPSSASPLPTEEGLEGEETLQLQEQPSNSSGKEVPM PSHNPSPASLESSPATTEKNSNFTVTPRSRKHSTPTFPTSTVLTGLTPPPWPLSPTASRTVKALAVSAGD NLVLTLPDREAELKASVEPAPPADTTYSYEWSLMSHPVDFQGKIKQENKPTLHLSQLSVGLYAFRVAVSS ENAFGEGYVNVTVMPAARVNQPPVAVVSPQTQELSVPLTSALIDGSQSTDDTEIVSYHWEEVDGPFLGEE FPADTPILRLSNLVPGNYTFRLTITDSDGATNSTTASLVIRDAVDYPPVANAGPNQTITLPQNTIILNGN QSSDDHQIVLYEWFAGPGGESKEMVMQGAQTPYLHLSELQEGEYTFQLMVTDSSGQQSTALVAVTVQAEN NQAPVAVAGPDKELVFPVQSATLDGSRSSDDHGIVCYHWEHIRGPSAVEMENVDKAIATVTGLQVGIYHF RLTVRDQQGLSSTSTLTVAVKKENNSPPRAQAGGRHVLILPNNSITLDGSRSTDDRGIVSYLWIRDGQSP AAGDVIGGSDHRAALQLTNLVEGVYTFHLLVTDSQGASDSDAATVEVLPDPKKDGLVELILQVGVEQLTE QQKETLVRQLAVLLNVLDSDVKVLKIQAHTDVSTVIVFYVQSGSPFKVLRAAAVARNLHKRLSKEKEAFL LFKVLRVDTAGCLLKCSGHGHCDPITKRCICSQLWMENLIQRYMWDGESNCEWSVFYVAALALTLTLLTG AVSWLCICCCRRRKRTKIRKKTKYTILDSMDEQERMELRPKYGIKHRSTEHNSSLMVSESEFESDQDTLF SRERMERGVLKGSLNGCARNGVSFGYYSKDR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081051 |
| ORF Size | 3243 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081051.1, NP_001074520.1 |
| RefSeq Size | 4977 bp |
| RefSeq ORF | 3246 bp |
| Locus ID | 210108 |
| UniProt ID | Q5SZV5 |
| Gene Summary | This gene encodes a transmembrane protein that contains a large extracellular domain with multiple polycystic kidney disease (PKD) domains. The encoded protein may play a role in the development of the cerebral cortex by regulating neuronal migration and cell adhesion. Single nucleotide polymorphisms in a similar gene in human are associated with dyslexia. Alternatively spliced transcript variants have been identifed. [provided by RefSeq, May 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223491 | D130043K22Rik (untagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik), (10ug) |
CNY 13,870.00 |
|
| MR218150 | D130043K22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik) |
CNY 7,536.00 |
|
| MR218150L3 | Lenti ORF clone of D130043K22Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik) |
CNY 11,400.00 |
|
| MR218150L4 | Lenti ORF clone of D130043K22Rik (mGFP-tagged) - Mouse RIKEN cDNA D130043K22 gene (D130043K22Rik) |
CNY 11,400.00 |
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