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Myo1c (NM_001080775) Mouse Tagged ORF Clone
CAT＃: MG218062
- TrueORF®
Myo1c (tGFP-tagged) - Mouse myosin IC (Myo1c) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001080775" in other vectors (3)
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CNY 11,500.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C80397; mm1bet; mm1beta; MMIb; MYO1E; myr; myr2; NMI |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218062 representing NM_001080775
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTACCGGGCATCGGCCCTGGGCAGTGACGGGGTTCGAGTGACCATGGAGAGCGCCTTGACTGCCC GAGACCGGGTAGGGGTGCAGGACTTTGTCCTGCTGGAGAATTTCACCAGTGAGGCTGCCTTCATTGAGAA CCTCCGGCGGCGGTTCCGGGAGAACCTCATTTATACCTACATCGGTCCTGTCCTAGTCTCTGTCAATCCC TACCGAGACCTACAGATCTACAGCCGGCAGCATATGGAACGCTACCGTGGTGTCAGTTTCTATGAAGTAC CACCTCATTTGTTTGCAGTGGCTGACACTGTATACCGGGCACTTCGTACTGAGCGTCGGGACCAGGCAGT GATGATTTCTGGAGAGAGTGGGGCAGGCAAGACAGAGGCCACCAAGAGACTGCTCCAGTTCTATGCAGAG ACCTGCCCAGCCCCTGAACGGGGTGGCGCAGTGCGAGACCGCCTGTTGCAGAGCAACCCCGTGTTAGAGG CCTTTGGGAATGCCAAGACTCTCCGCAACGATAACTCCAGCCGGTTTGGAAAGTACATGGATGTGCAGTT TGACTTCAAGGGTGCCCCCGTGGGAGGCCACATTCTCAGTTACCTCCTGGAAAAGTCCCGGGTGGTGCAC CAAAATCACGGAGAGCGGAACTTCCACGTCTTTTACCAGCTACTGGAGGGGGGCGAGGAGGAGACTCTCC GTCGGCTGGGCTTGGAACGGAACCCCCAGAGCTACTTGTACCTGGTGAAGGGCCAGTGTGCCAAGGTCTC CTCCATCAACGACAAGAGTGACTGGAAGGTTATGAGGAAGGCGCTGTCCGTCATTGACTTCACTGAGGAT GAAGTGGAGGACTTGCTCAGCATCGTGGCCAGCGTCCTACATCTGGGCAACATCCACTTTGCTGCTGACG AGGACAGCAATGCCCAGGTTACTACTGAGAACCAGCTCAAATATCTGACCAGGCTCCTTGGTGTGGAAGG TACAACACTTAGGGAAGCCCTGACCCACAGGAAGATCATCGCCAAGGGGGAAGAGCTCCTGAGCCCACTG AACCTTGAACAGGCGGCATATGCAAGGGATGCGCTTGCCAAGGCTGTGTACAGCCGGACATTCACCTGGC TGGTCAGAAAGATCAATAGGTCACTGGCCTCTAAGGACGCTGAGAGCCCCAGCTGGCGAAGCACCACGGT TCTTGGGCTCCTGGACATTTACGGCTTTGAAGTGTTTCAGCATAACAGCTTCGAGCAGTTCTGCATCAAC TACTGCAATGAGAAGCTGCAGCAGCTCTTCATCGAGCTGACTCTCAAGTCGGAGCAGGAGGAATACGAGG CTGAGGGCATCGCGTGGGAACCTGTCCAGTACTTCAACAACAAGATCATCTGTGACCTGGTAGAGGAGAA GTTCAAGGGCATCATCTCCATCTTGGATGAAGAGTGCCTGCGTCCTGGGGAGGCCACGGACCTGACCTTT CTGGAGAAGTTGGAGGACACTGTCAAGCCCCACCCTCACTTCCTGACGCACAAGCTCGCTGACCAGAAGA CCAGGAAATCTCTAGACCGAGGGGAGTTCCGCCTTCTGCATTATGCTGGAGAGGTGACCTACAGTGTGAC TGGGTTTCTGGATAAAAACAATGACCTCCTCTTCCGGAACCTGAAGGAGACCATGTGCAGCTCAATGAAC CCCATCATGGCCCAGTGCTTTGACAAGAGTGAGCTCAGTGACAAGAAGCGGCCAGAGACGGTGGCCACCC AGTTCAAGATGAGCCTCCTGCAGCTCGTGGAGATCCTGAGGTCTAAGGAGCCTGCCTATATCCGGTGCAT CAAGCCAAACGACGCCAAGCAGCCGGGTCGCTTTGATGAGGTGCTCATCCGACATCAGGTGAAGTACCTG GGACTGATGGAGAATCTGCGCGTGCGCAGAGCTGGCTTTGCCTATCGTCGCAAATATGAGGCTTTCCTGC AGAGGTACAAGTCACTGTGCCCAGAGACATGGCCCATGTGGGCAGGACGGCCCCAGGATGGTGTGGCCGT GTTGGTCAGACACCTCGGCTACAAGCCAGAAGAGTACAAAATGGGCAGGACTAAGATCTTCATCCGATTT CCCAAGACCTTGTTTGCCACAGAGGACTCCCTGGAAGTCCGGCGGCAGAGTCTAGCCACCAAGATCCAGG CGGCCTGGAGGGGCTTTCATTGGCGACAGAAATTTCTCCGGGTGAAGCGATCAGCCATCTGTATCCAGTC ATGGTGGCGTGGCACACTGGGCCGGAGGAAGGCAGCCAAGAGGAAGTGGGCAGCCCAGACCATCCGTCGA CTCATCCGTGGCTTCATTTTGCGCCATTCACCCCGGTGCCCTGAGAATGCCTTCTTCTTGGACCACGTGC GCGCCTCATTTTTGCTTAACCTGAGGCGGCAACTGCCCCGGAATGTTCTGGACACCTCCTGGCCCACACC CCCACCTGCCCTGAGAGAGGCCTCAGAACTGCTACGGGAACTGTGCATGAAGAACATGGTGTGGAAGTAC TGCCGGAGCATCAGCCCTGAGTGGAAGCAGCAGCTGCAGCAAAAGGCGGTGGCTAGTGAAATTTTCAAGG GCAAGAAGGACAACTACCCCCAGAGTGTCCCCAGACTCTTCATTAGCACACGGCTTGGCACAGAGGAGAT CAGCCCCAGAGTGCTTCAATCCTTGGGCTCTGAACCCATCCAGTATGCCGTGCCCGTGGTAAAATACGAC CGTAAGGGTTACAAGCCTCGCCCCCGGCAGCTGCTGCTCACGCCCAGTGCTGTGGTCATTGTGGAGGATG CTAAAGTCAAGCAGAGAATTGATTATGCCAACCTAACCGGAATCTCTGTCAGTAGCCTGAGTGATAGCCT ATTTGTGCTTCACGTGCAGCGTGAAGACAACAAGCAGAAGGGAGATGTGGTGCTGCAGAGTGATCATGTG ATCGAGACACTAACCAAGACGGCCCTCAGTGCTGACCGCGTGAACAATATCAACATCAACCAGGGCAGCA TAACGTTTGCAGGGGGTCCAGGCAGGGACGGCATCATTGACTTCACATCGGGCTCAGAGCTTCTCATCAC CAAGGCTAAGAATGGCCACCTGGCTGTGGTGGCCCCACGGCTGAATTCTCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218062 representing NM_001080775
Red=Cloning site Green=Tags(s) MRYRASALGSDGVRVTMESALTARDRVGVQDFVLLENFTSEAAFIENLRRRFRENLIYTYIGPVLVSVNP YRDLQIYSRQHMERYRGVSFYEVPPHLFAVADTVYRALRTERRDQAVMISGESGAGKTEATKRLLQFYAE TCPAPERGGAVRDRLLQSNPVLEAFGNAKTLRNDNSSRFGKYMDVQFDFKGAPVGGHILSYLLEKSRVVH QNHGERNFHVFYQLLEGGEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVMRKALSVIDFTED EVEDLLSIVASVLHLGNIHFAADEDSNAQVTTENQLKYLTRLLGVEGTTLREALTHRKIIAKGEELLSPL NLEQAAYARDALAKAVYSRTFTWLVRKINRSLASKDAESPSWRSTTVLGLLDIYGFEVFQHNSFEQFCIN YCNEKLQQLFIELTLKSEQEEYEAEGIAWEPVQYFNNKIICDLVEEKFKGIISILDEECLRPGEATDLTF LEKLEDTVKPHPHFLTHKLADQKTRKSLDRGEFRLLHYAGEVTYSVTGFLDKNNDLLFRNLKETMCSSMN PIMAQCFDKSELSDKKRPETVATQFKMSLLQLVEILRSKEPAYIRCIKPNDAKQPGRFDEVLIRHQVKYL GLMENLRVRRAGFAYRRKYEAFLQRYKSLCPETWPMWAGRPQDGVAVLVRHLGYKPEEYKMGRTKIFIRF PKTLFATEDSLEVRRQSLATKIQAAWRGFHWRQKFLRVKRSAICIQSWWRGTLGRRKAAKRKWAAQTIRR LIRGFILRHSPRCPENAFFLDHVRASFLLNLRRQLPRNVLDTSWPTPPPALREASELLRELCMKNMVWKY CRSISPEWKQQLQQKAVASEIFKGKKDNYPQSVPRLFISTRLGTEEISPRVLQSLGSEPIQYAVPVVKYD RKGYKPRPRQLLLTPSAVVIVEDAKVKQRIDYANLTGISVSSLSDSLFVLHVQREDNKQKGDVVLQSDHV IETLTKTALSADRVNNININQGSITFAGGPGRDGIIDFTSGSELLITKAKNGHLAVVAPRLNSR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001080775 |
| ORF Size | 3132 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001080775.1, NP_001074244.1 |
| RefSeq Size | 5311 bp |
| RefSeq ORF | 3135 bp |
| Locus ID | 17913 |
| UniProt ID | Q9WTI7 |
| Gene Summary | This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The protein functions in intracellular vesicle transport to the plasma membrane. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR218062 | Myo1c (Myc-DDK-tagged) - Mouse myosin IC (Myo1c), transcript variant 1 |
CNY 10,450.00 |
|
| MR218062L3 | Lenti ORF clone of Myo1c (Myc-DDK-tagged) - Mouse myosin IC (Myo1c), transcript variant 1 |
CNY 12,350.00 |
|
| MR218062L4 | Lenti ORF clone of Myo1c (mGFP-tagged) - Mouse myosin IC (Myo1c), transcript variant 1 |
CNY 12,350.00 |
