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Ttbk2 (NM_080788) Mouse Tagged ORF Clone
CAT＃: MG217963
- TrueORF®
Ttbk2 (tGFP-tagged) - Mouse tau tubulin kinase 2 (Ttbk2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_080788" in other vectors (4)
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CNY 12,730.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610507N02Rik; AI326283; B930008N24Rik; mKIAA0847; Ttbk; Ttbk1; TTK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217963 representing NM_080788
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTAGCCAACCGACGCCACAGGCTGGGAACGTGCTCTCCAATCAGCACCCGCGGCGGAGAGAATT GTAAAACCGGTAAGATCAAGTGCCTATTGAAAGAGACTCACTCTTGGCTAAGAAGAGAAATAGGAAATAG GTTAAATGGAATCCACCCTTGGGAGCTAGATGCCTGTGTAGCTGTTTTACCTCATCAGGGTTTTGCAATG AGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGTTAA GAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGTGGC GCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAACTG CAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCATGC AATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCACTAC CCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGAGAC ATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATTTTG GCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCGAGG GACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCT TTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGCAAG TAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCACCTT TCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTTGAC AATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATGGCT CCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGGAAT TGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAACAG CTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGCACC CACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAATTTG CAAAGCAGCTACTGAAGAAGAAAATAGCCATGGTCAAGTAAATGGCATACTCAATGCTCCAAGCCTTGGT TCACCAATTCGTGTCCGATCAGAGATTACTCAGCCAGACAGAGATGTTCCGTTAGTAAGGAAGCTACGTT CTATTCACAGCTTTGAGCTGGAAAAACGTTTGACACTTGAGCCAAAGCCAGATACTGACAAGTTTCTGGA GACCTGCATGGAGAAAATGCAGAAAGATTCCAGTGCAGGAAAAGAACCTGTCCCCCCTGCTCTGCCTCAT AAGCCGTGTGTCCCCGTCGTGACCCACACTGACCACATCTGGCACTATGATGACGAATATCTTCCTGATG CCTCCAAGCCTGCCTCTGCCAACACCCCGGAGCAGGCAGATGGTGGTGGCAGCAACGGATTTATAGCTGT TAACTTAAGCTCTTGCAAACAGGAGGTTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAAGACCTT CAGGACTTTAGGACAAATGAGGTGTTAGGCCATAAGACAACTGGAAGCCCTTCAGATGAGGAGCCTGAAG TGCTTCAGGTCCTTGAGGGATCACCTCAAGATGAAAAGATCCAAGTAGGTCCTTGGACTGACAACCATCA CTTAAAGAAGGAAAGCTCAGGTGTGGTTTTAGCACTTTCTGCTGAATGCCCTGCTACTGCTGCTTCAGAA CTGTACACAGATAGGCTAGACCTCCAGGCTGGAGCTGCAAGTCAGTTCATCACAGTGACTCCCACAAGTC CCATGGAGGCACAAGCAGAAGGACCCCTGACTGCGATTACAATTCCTAGACCTTCTGTGGCATCAACACA GTCAACTTCAGGAAGCTTTCACTATGGCCCACAACCAGAGAAGAAAGATCTTCAGCCCTTGGAGCCCACT GTAGAACTGTACTCTCCAAGGGAGAACTTCTCCGGCTTGGTTGTGACAGAGGGTGAACTAGCTAGCGGAG GAAGCAGAGTGGATTTGGGACTTCAGATAGATCACACTGGTCATGACATGTTACCCAACATGAGAGATGG TGACACATCTCAAGACTTGGGACCAAAAGACCCTCCTGACCATAATAGATTAGCTGTGAAAGAATTTGAA CATCTCCCTGGAGAAACAGAAGAGAGAAGCCTTCTTCTGGGCTCAGAGAATGAAGATGAGAGGTTAAGTA AAGGGCAGCACTGTATTGAAGTCTCTTCCCCAGGAGAGTTAGTGACTGCAGAGAGGGCTCAGTTAGCTGC CACAGAACCTCTGCATGTATCAGAGACACAGAACTGTAGTGTGCTACCAAATCAGGACAAAACCCATGAG ATAATGAAGCTTTTGGCAGTTGGAACTTCAGAAATTTCTCCTCAAGCCATTGACCCACATGCTGAAGGGC AGATAGGCCAGATGGCAGCAATGCAGAAAAATAAGCTATTTAAAGATGATGGTATTCAGAGTGAAAGCTT GCCAAGGCAGCAGGGAGACCTCTCTGCTTTTTTGCACCAAGAGGGTAAGAGAGAGAAAGTTGTCCCTAGA AATGGAGAGCTCTATCATTGTGTCTCAGAGAATGAGCATGGTCCTCCTACTCGGAAGGACATGCTCCGGT CATCCTTTGTGACCAGGCACAGCCGGATCCCTGTTTTAGCACAAGAAATAGACTCAACTTTTGAATCATC CTCTGCTATTTCTGCAAAAGAAAAGCTTCTACAGAAGAAAGCCTATCAGCCAGAAATAGTCAAACTTCTT GTAGAAAAAAGGCAGTTCAAGTCTTTCCTGGGAGACCTCTCAAGTGCCTCTGATAAGCTGATAGAGGAGA AACTAGCCGCTGTTCCAGTCCCTTTTTCTGAGGAGGAAGTCTTCGCTCCCTTTTCTAGACTGGCAGCAGA TTCCCACCTGAGTAGATCAGTTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGGCTAGAAAGAGCAAG ATTCCAAGGCCAGTATCCTGGGTCAGCACAGATCAAATTAATGGCTCCGCTTCACCTCAGTTCTTGCCTC GGCCACCACCAGGAAAGCCACCAGTCAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTTCTAGG GAGTAGTAACTCTGACTCAGACCTTTTCTCTCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAATCC CGGAGTACTACCCAATGCAAGAGCCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTG TACCTCGAAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGAACATCTAGTTCCTCACCATC TAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCTTCCCCACATCTGGGGAGAAGCAAGTCACCCCCT AGCCACTCAGGATCATCGTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCC CAAAAGGATCTGGCAGCCTCCACCACCACTCAACCAGCTCTAAAACTCCCCCAGGGAAGAGTAAGCCAGC CAGTAAACTCAGCAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217963 representing NM_080788
Red=Cloning site Green=Tags(s) MGLANRRHRLGTCSPISTRGGENCKTGKIKCLLKETHSWLRREIGNRLNGIHPWELDACVAVLPHQGFAM SGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKL QGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGKQILESIESIHSVGFLHRD IKPSNFAMGRFPSTCRKCFMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWS LFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSTFLDHISSLDYFTKPDYQLLTSVFD NSIKTFGVIESDPFDWEKSGTDGSLTTTTTSATPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQ LSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDINKNKIKLGICKAATEEENSHGQVNGILNAPSLG SPIRVRSEITQPDRDVPLVRKLRSIHSFELEKRLTLEPKPDTDKFLETCMEKMQKDSSAGKEPVPPALPH KPCVPVVTHTDHIWHYDDEYLPDASKPASANTPEQADGGGSNGFIAVNLSSCKQEVDSKEWVIVDKEQDL QDFRTNEVLGHKTTGSPSDEEPEVLQVLEGSPQDEKIQVGPWTDNHHLKKESSGVVLALSAECPATAASE LYTDRLDLQAGAASQFITVTPTSPMEAQAEGPLTAITIPRPSVASTQSTSGSFHYGPQPEKKDLQPLEPT VELYSPRENFSGLVVTEGELASGGSRVDLGLQIDHTGHDMLPNMRDGDTSQDLGPKDPPDHNRLAVKEFE HLPGETEERSLLLGSENEDERLSKGQHCIEVSSPGELVTAERAQLAATEPLHVSETQNCSVLPNQDKTHE IMKLLAVGTSEISPQAIDPHAEGQIGQMAAMQKNKLFKDDGIQSESLPRQQGDLSAFLHQEGKREKVVPR NGELYHCVSENEHGPPTRKDMLRSSFVTRHSRIPVLAQEIDSTFESSSAISAKEKLLQKKAYQPEIVKLL VEKRQFKSFLGDLSSASDKLIEEKLAAVPVPFSEEEVFAPFSRLAADSHLSRSVEDSFLSPIISQARKSK IPRPVSWVSTDQINGSASPQFLPRPPPGKPPVRPGVEARLRRYKVLGSSNSDSDLFSRLAQILQNGSQKS RSTTQCKSPGSPHNPKTPPKSPVVPRRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPP SHSGSSSSRRSCQQEHCKPSKNGPKGSGSLHHHSTSSKTPPGKSKPASKLSR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080788 |
| ORF Size | 3936 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_080788.3, NP_542966.2 |
| RefSeq Size | 10906 bp |
| RefSeq ORF | 3939 bp |
| Locus ID | 140810 |
| UniProt ID | Q3UVR3 |
| Gene Summary | Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224225 | Ttbk2 (untagged) - Mouse tau tubulin kinase 2 (Ttbk2), transcript variant 1, (10ug) |
CNY 16,910.00 |
|
| MR217963 | Ttbk2 (Myc-DDK-tagged) - Mouse tau tubulin kinase 2 (Ttbk2), transcript variant 1 |
CNY 9,136.00 |
|
| MR217963L3 | Lenti ORF clone of Ttbk2 (Myc-DDK-tagged) - Mouse tau tubulin kinase 2 (Ttbk2), transcript variant 1 |
CNY 13,490.00 |
|
| MR217963L4 | Lenti ORF clone of Ttbk2 (mGFP-tagged) - Mouse tau tubulin kinase 2 (Ttbk2), transcript variant 1 |
CNY 13,490.00 |
