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Cdc42bpa (NM_001033285) Mouse Tagged ORF Clone
CAT＃: MG217902
- TrueORF®
Cdc42bpa (tGFP-tagged) - Mouse CDC42 binding protein kinase alpha (Cdc42bpa), (10ug)
ORF Plasmid: DDK
"NM_001033285" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
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经常一起买 (4)
Mouse monoclonal turboGFP antibody, clone OTI2H8
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A930014J19Rik; DMPK-like |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217902 representing NM_001033285
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGAGAAGTGCGTTTGAGGCAGTTGGAGCAGTTTATTTTAGATGGGCCAGCTCAGACCAATGGGC AGTGCTTCAGTGTGGAGACGCTACTGGACATCCTCATCTGTCTTTACGATGAATGCAATAATTCTCCATT GAGAAGAGAGAAGAACATTCTTGAATATCTAGAATGGGCTAAACCATTTACTTCTAAAGTGAAGCAAATG CGATTACATAGAGAAGACTTTGAAATATTAAAGGTGATTGGTCGAGGAGCTTTTGGGGAGGTTGCTGTAG TTAAACTAAAAAATGCAGATAAAGTATTTGCCATGAAAATTCTGAACAAGTGGGAAATGCTGAAAAGAGC TGAGACAGCGTGCTTTCGTGAAGAGAGGGATGTGTTGGTAAATGGAGACAGTAAGTGGATAACCACTCTG CACTATGCTTTCCAGGATGACAATAACTTATACCTGGTTATGGATTATTATGTTGGTGGGGATTTACTTA CTCTGCTCAGCAAATTTGAAGATAGATTGCCTGAAGAGATGGCTCGGTTTTACTTGGCTGAGATGGTGAT AGCCATTGACTCAGTTCACCAGCTCCACTATGTTCACAGAGACATCAAACCTGACAATATATTGATGGAT ATGAATGGACATATTCGTTTAGCAGATTTTGGTTCTTGTCTGAAGCTGATGGAAGATGGAACGGTCCAGT CCTCGGTGGCAGTTGGAACTCCAGATTATATTTCTCCGGAAATCCTTCAGGCTATGGAAGACGGAAAAGG CAGATATGGACCAGAGTGTGACTGGTGGTCCTTGGGGGTCTGTATGTATGAGATGCTTTACGGAGAAACA CCATTCTATGCAGAGTCTCTGGTGGAGACATATGGAAAAATTATGAATCACAAAGAGCGGTTTCAGTTTC CAGCCCAAGTGACAGACGTGTCCGAAAATGCTAAAGATCTTATTCGAAGACTCATTTGTAGTAGAGAACA TCGACTTGGCCAAAATGGAATAGAAGACTTTAAGAAGCATCCATTCTTCAGTGGAATTGATTGGGATAAC ATTCGGAATTGTGAAGCACCTTACATCCCAGAAGTTAGTAGCCCGACAGATACGTCAAATTTTGATGTGG ATGACGACTGTTTAAAAAATTCTGAAACAATGCCCCCACCAACACATACTGCATTTTCTGGCCATCATCT ACCATTTGTTGGTTTTACATATACTAGTAGCTGTGTTCTTTCTGATCGGAGCTGTTTAAGAGTCACAGCA GGCCCCACCTCATTGGACCTTGATGTCAGTGTCCAGAGGACTCTAGACAATAACTTAGCAACTGAGGCTT ATGAAAGGAGAATTAAGCGGCTTGAGCAGGAAAAGCTTGAACTGACTAGAAAACTTCAAGAGTCAACACA GACTGTTCAAGCTCTGCAATATTCAACTGTTGATGGTCCACTAACGGCAAGCAAAGATTTAGAAATTAAA AGTCTGAAAGAAGAGATTGAAAAACTAAGGAAACAAGTAGCAGAAGTGAATCATTTGGAACAACAACTTG AAGAAGCTAATTCTGTGAGACGAGAACTAGATGATGCTTTCAGGCAAATCAAAGCTTCTGAAAAACAAAT CAAAACTTTGCAGCAGGAAAGAGAAGAACTAAATAAGGAACTAGTCCAGGCTAGTGAGCGATTAAAAAAC CAATCCAAAGAGCTGAAAGATGCACACTGTCAGAGGAAACTGGCCATGCAGGAATTCATGGAGATCAATG AGCGACTAACAGAATTGCACACCCAAAAGCAGAAACTTGCTCGCCATGTCCGAGATAAGGAAGAAGAGGT GGACCTGGTGATGCAAAAAGCTGAGAGCTTAAGGCAGGAGCTGCGCAGAGCAGAAAGAGCCAAGAAAGAG CTGGAAGTTCACACCGAAGCTCTAATTGCTGAAGCATCAAAGGACAAGAAGCTCCGAGAGCAGAGCGAAC ATTATTCTAAGCAACTGGAAAACGAGTTGGAGGGCCTGAAGCAAAAACAAATTAGTTACTCACCAGGAAT ATGCAGCATAGAACATCAGCAAGAGATAACTAAACTGAAAACTGACTTGGAAAAGAAAAGTATCTTTTAT GAAGAAGAAATTTCTAAAAGAGAAGGAATTCACGCAAGTGAGATTAAAAACCTGAAGAAGGAGCTGCATG ACTCAGAAGGCCAGCAGCTTGCTCTCAACAAAGAGATTCTGGTTTTAAAAGACAAATTGGAAAAAACCAG GAGAGAAAGTCAAAGTGAAAGGGAAGAATTTGAAAATGAGTTCAAACAACAATATGAACGGGAAAAAGTA TTATTAACTGAAGAGAATAAAAAGTTAACGAGTGAACTCGATAAGCTGACTTCACTGTATGAGAGCTTAA GTTTGCGCAACCAGCACTTAGAAGAAGAAGTCAAAGATCTAGCAGATAAGAAGGAATCAGTTGCCCACTG GGAAGCCCAAATCACAGAGATAATTCAGTGGGTGAGCGATGAGAAGGATGCACGAGGCTATCTTCAGGCC TTAGCTTCTAAAATGACTGAAGAGTTGGAAGCATTAAGAAATTCCAGCTTGGGGACACGAGCAACAGATA TGCCCTGGAAAATGCGTCGTTTTGCAAAACTGGACATGTCGGCTAGACTAGAGTTGCAGTCTGCTCTGGA TGCAGAAATAAGAGCTAAGCAGGCCATCCAGGAAGAGCTGAATAAAGTCAAAGCATCTAACATCTTAACA GAATGTAAACTGAAAGATTCAGAGAAGAAGAACTTGGAACTGTTGTCCGAAATCGAGCAGCTGATAAAGG ACACTGAAGAGCTTAGATCTGAAAAGGGTATAGAGCACCAAGACTCACAGCATTCTTTCTTGGCATTTTT GAATACGCCTACCGATGCTCTGGACCAATTTGAACGCTCTCCGTCCTGTACGCCAGCTAGCAAAGGCAGA CGTATTGCAGACTGTGCTCCACTTCCAGCTCACACACCAACCTTAAGGAAAAAGGGATGCCCGGCTTCAA CTGGCTTTCCACCTAAGCGCAAGACTCATCAATTTTTCGTGAAGTCTTTCACTGCCCCTACCAAGTGTCA CCAGTGCACCTCCTTGATGGTCGGTTTGATAAGACAGGGCTGTTCCTGTGAAGTCTGTGGGTTCTCATGT CATATAACTTGTGTGAACAAAGCCCCGACGGTTTGCCCAGTTCCTCCTGAGCAAACTAAAGGTCCACTGG GCATAGACCCCCAGAAGGGAGTTGGGACGGCGTATGAGGGTCATGTCAGGATCCCTAAGCCGGCGGGAGT GAAGAAAGGGTGGCAGAGAGCACTGGCTGTGGTCTGCGACTTCAAACTGTTTCTTTATGATATCGCAGAG GGAAAAGCATCGCAGCCCACCTCCGTCATTAGTCAAGTGATTGACATGAGAGATGAAGAATTTTCTGTGA GTTCAGTCTTAGCTTCTGATGTTATTCACGCAAGTCGAAAAGATATCCCCTGTATATTCAGGGTCACAGC TTCCCAGCTCTCAGCACCTAGTAACAAATGTTCCATCCTGATGCTAGCAGACAGCGAGAATGAGAGGAGT AAGTGGGTGGGAGTGCTGAGTGAACTCCACAAGATTCTGAAGAAAAACAAGTTCAGAGACCGCTCAGTGT ATGTCCCCAAGGAGGCGTACGACAGCACGCTACCCCTCATTAAGACAACGCAGGCCGCTGCAATCATAGA TCATGAACGGATAGCTTTGGGAAATGAAGAAGGATTATTTGTTGTGCATGTCACCAAAGATGAGATTGTT AGAGTTGGTGATAATAAGAAAATCCATCAGATCGAACTCATTCCAAGTGACCAGCTTGTCGCTGTGATCT CAGGCCGAAATCGCCACGTCCGACTTTTTCCTATGTCAGCCTTGGATGGGCGAGAGACGGATTTTTATAA ACTGGCAGAGACTAAAGGGTGTCAGACCATAGCTGCTGGGAAAGTGCGCCACGGAGCTCTGTCCTGCCTG TGTGTGGCCATGAAGAGACAGGTCCTCTGCTATGAACTGTTCCAAAGCAAGACCAGGCACAGGAAGTTTA AGGAGATTCAAGTCCCATGTAATGTCCAGTGGATGGCCATCTTCAGTGAGCATCTCTGTGTGGGATTCCA GTCAGGATTTCTCAGATACCCCCTGAATGGAGAAGGAGGTCCATGCAATATGCTCCATTCAAATGACCAT ACACTGTCATTCATTTCACACCAACCAATGGACGCTCTCTGTGCAGTTGAGATCTCCAACAAAGAGTACT TGCTGTGTTTTAACAGCATTGGGATATACACAGACTGCCAGGGCCGAAGATCCAGACAGCAGGAACTGAT GTGGCCAGCAAATCCTTCCTCCTGTTGTTACAATGCTCCATATCTCTCCGTGTATAGTGAAAACGCAGTT GATATCTTTGATGTAAACTCCATGGAATGGATACAGACTCTTCCTCTCAAAAAGGTCCGACCTTTAAACA CTGAAGGATCATTAAATCTCTTAGGATTGGAGACCATTAGATTAATATATTTCAAAAATAAGATGGCAGA AGGGGATGAACTTGTGGTTCCTGAAACATCAGATAATAGTCGGAAACAAATGGTTAGAAATATCAACAAC AAACGGCGTTACTCCTTCCGAGTCCCTGAAGAAGAGAGGATGCAACAGAGGAGGGAGATGCTACGAGATC CAGAAATGAGAAATAAATTAATTTCTAACCCAACTAATTTTAACCACATAGCACACATGGGTCCTGGAGA TGGAATACAGATCCTAAAAGACCTGCCCATGAACCCACGGCCTCAGGAAAGCCGGACAGTATTCAGTGGC TCCGTCAGTATTCCCTCCATCACCAAGTCCCGCCCTGAGCCAGTCCGCTCCATGAGTGCCAGCAGCGGCC TGTCTGCACGGTCGTCAGCCCAGAATGGCAGTGCACTAAAGAGGGAATTCTCTGGAGGAAGCTATAACAC AAAGCGACAGCCCATGCCCTCTCCCTCGGAGGGCTCTTTGTCATCAGGAGGCATGGACCAAGGAAGTGAC GCCCCTGCGAGGGACTATGATGGAGAGGACTCTGATTCTCCAAGGCATTCCACAGCTTCCAACAGCTCTA ACCTGAGCAGCCCCCCAAGTCCCATTTCACCCCAAAAGACCAAGAGCCTCTCCCTGGAGAGCACAGACCG CGGGAGCTGGGATCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217902 representing NM_001033285
Red=Cloning site Green=Tags(s) MSGEVRLRQLEQFILDGPAQTNGQCFSVETLLDILICLYDECNNSPLRREKNILEYLEWAKPFTSKVKQM RLHREDFEILKVIGRGAFGEVAVVKLKNADKVFAMKILNKWEMLKRAETACFREERDVLVNGDSKWITTL HYAFQDDNNLYLVMDYYVGGDLLTLLSKFEDRLPEEMARFYLAEMVIAIDSVHQLHYVHRDIKPDNILMD MNGHIRLADFGSCLKLMEDGTVQSSVAVGTPDYISPEILQAMEDGKGRYGPECDWWSLGVCMYEMLYGET PFYAESLVETYGKIMNHKERFQFPAQVTDVSENAKDLIRRLICSREHRLGQNGIEDFKKHPFFSGIDWDN IRNCEAPYIPEVSSPTDTSNFDVDDDCLKNSETMPPPTHTAFSGHHLPFVGFTYTSSCVLSDRSCLRVTA GPTSLDLDVSVQRTLDNNLATEAYERRIKRLEQEKLELTRKLQESTQTVQALQYSTVDGPLTASKDLEIK SLKEEIEKLRKQVAEVNHLEQQLEEANSVRRELDDAFRQIKASEKQIKTLQQEREELNKELVQASERLKN QSKELKDAHCQRKLAMQEFMEINERLTELHTQKQKLARHVRDKEEEVDLVMQKAESLRQELRRAERAKKE LEVHTEALIAEASKDKKLREQSEHYSKQLENELEGLKQKQISYSPGICSIEHQQEITKLKTDLEKKSIFY EEEISKREGIHASEIKNLKKELHDSEGQQLALNKEILVLKDKLEKTRRESQSEREEFENEFKQQYEREKV LLTEENKKLTSELDKLTSLYESLSLRNQHLEEEVKDLADKKESVAHWEAQITEIIQWVSDEKDARGYLQA LASKMTEELEALRNSSLGTRATDMPWKMRRFAKLDMSARLELQSALDAEIRAKQAIQEELNKVKASNILT ECKLKDSEKKNLELLSEIEQLIKDTEELRSEKGIEHQDSQHSFLAFLNTPTDALDQFERSPSCTPASKGR RIADCAPLPAHTPTLRKKGCPASTGFPPKRKTHQFFVKSFTAPTKCHQCTSLMVGLIRQGCSCEVCGFSC HITCVNKAPTVCPVPPEQTKGPLGIDPQKGVGTAYEGHVRIPKPAGVKKGWQRALAVVCDFKLFLYDIAE GKASQPTSVISQVIDMRDEEFSVSSVLASDVIHASRKDIPCIFRVTASQLSAPSNKCSILMLADSENERS KWVGVLSELHKILKKNKFRDRSVYVPKEAYDSTLPLIKTTQAAAIIDHERIALGNEEGLFVVHVTKDEIV RVGDNKKIHQIELIPSDQLVAVISGRNRHVRLFPMSALDGRETDFYKLAETKGCQTIAAGKVRHGALSCL CVAMKRQVLCYELFQSKTRHRKFKEIQVPCNVQWMAIFSEHLCVGFQSGFLRYPLNGEGGPCNMLHSNDH TLSFISHQPMDALCAVEISNKEYLLCFNSIGIYTDCQGRRSRQQELMWPANPSSCCYNAPYLSVYSENAV DIFDVNSMEWIQTLPLKKVRPLNTEGSLNLLGLETIRLIYFKNKMAEGDELVVPETSDNSRKQMVRNINN KRRYSFRVPEEERMQQRREMLRDPEMRNKLISNPTNFNHIAHMGPGDGIQILKDLPMNPRPQESRTVFSG SVSIPSITKSRPEPVRSMSASSGLSARSSAQNGSALKREFSGGSYNTKRQPMPSPSEGSLSSGGMDQGSD APARDYDGEDSDSPRHSTASNSSNLSSPPSPISPQKTKSLSLESTDRGSWDP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033285 |
| ORF Size | 5196 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033285.1, NP_001028457.1 |
| RefSeq Size | 8656 bp |
| RefSeq ORF | 5199 bp |
| Locus ID | 226751 |
| Gene Summary | Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration. Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2. In concert with MYO18A and LRP35A, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. Phosphorylates: PPP1R12A and LIMK2. May play a role in TFRC-mediated iron uptake (By similarity). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (PubMed:25107909).[UniProtKB/Swiss-Prot Function] |
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