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Map3k12 (NM_001163643) Mouse Tagged ORF Clone
CAT＃: MG217856
- TrueORF®
Map3k12 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 12 (Map3k12) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001163643" in other vectors (3)
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CNY 11,352.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DLK; MUK; Zpk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217856 representing NM_001163643
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGCCTCCATGAAACCCGAACACCCTCCCCTTCCTTTGGGGGCTTTGTGTCTACTCTAAGTGAGG CTTCTATGCGAAAGCTGGATCCAGACACTTCAGACTGCACCCCCGAGAAAGACCTGACGCCCACCCAATG TGTACTTCGAGATGTTGTGCCTCTCGGTGGGCAGGGTGGAGGAGGACCCAGCCCCTCCCCAGGTGGAGAG CCTCCCCCAGAGCCTTTTGCCAATAGTGTCCTCCAGCTACATGAGCAGGATACAGGTGGGCCAGGGGGAG CCACTGGGTCACCTGAGAGTCGAGCATCTAGAGTTCGAGCTGATGAGGTACGTCTGCAGTGCCAGAGTGG CAGTGGCTTCCTTGAAGGTCTTTTTGGCTGCCTGCGCCCTGTCTGGACAATGATTGGCAAAGCCTACTCC ACAGAACACAAGCAACAGCAGGAAGACCTTTGGGAAGTCCCCTTTGAGGAAATCCTGGACCTGCAGTGGG TAGGCTCAGGGGCTCAGGGTGCTGTTTTCCTGGGACGCTTCCACGGGGAGGAGGTAGCTGTGAAGAAGGT TCGAGATCTCAAGGAGACTGACATCAAGCATCTTCGAAAGCTGAAGCACCCCAACATCATCACTTTCAAG GGTGTTTGCACACAGGCCCCCTGCTACTGCATCCTTATGGAATTCTGCGCTCAAGGACAGCTATATGAGG TGCTCAGAGCCGGCCGCCCTGTCACCCCCTCCTTGCTGGTTGACTGGTCCATGGGCATCGCTGGTGGCAT GAATTACCTGCACCTGCACAAGATTATCCACAGAGACCTGAAGTCACCCAACATGCTAATCACATACGAC GATGTGGTGAAGATCTCAGATTTTGGCACTTCCAAGGAGCTGAGTGACAAGAGCACCAAGATGTCCTTTG CAGGAACAGTAGCCTGGATGGCTCCTGAAGTGATCAGAAATGAACCTGTGTCTGAGAAGGTTGACATCTG GTCCTTTGGGGTGGTGCTATGGGAACTACTGACTGGAGAGATTCCCTACAAAGATGTAGATTCCTCAGCC ATCATCTGGGGTGTGGGAAGCAACAGTCTCCACCTGCCTGTACCCTCCAGCTGCCCAGATGGTTTTAAAA TTCTACTTCGCCAGTGCTGGAACAGCAAACCACGAAATCGCCCATCATTCCGACAGATCTTGCTGCACCT GGACATCGCCTCCGCTGATGTGCTCTCTACACCCCAGGAGACTTACTTTAAGTCCCAGGCAGAGTGGCGG GAAGAAGTAAAACTGCACTTTGAAAAGATTAAGTCAGAAGGGACCTGTCTGCACCGCCTAGAAGAGGAAC TGGTGATGCGGAGAAGGGAGGAGCTCAGACATGCCCTGGACATCAGGGAGCACTATGAACGGAAGTTGGA GAGAGCCAACAACCTGTACATGGAACTGAATGCCCTCATGCTGCAACTAGAACTCAAAGAGAGGGAATTG CTCAGGCGAGAGCAGGCTTTAGAAAGGCGGTGTCCTGGTCTACTAAAGTCACACCCTTCTCGGGGCCTCC TACATGGAAACACTATGGAGAAGCTCATCAAGAAAAGGAACGTGCCACAGAAACTGTCGCCCCACAGCAA AAGGCCAGATATTCTCAAGACAGAGTCGTTGCTACCTAAACTAGATGCAGCCCTAAGTGGGGTGGGGCTT CCTGGGTGTCCTAAGGGCCCCCCTTCACCTGGAAGGAGTCGGCGTGGCAAGACCCGTCACCGAAAGGCCA GTGCCAAGGGCAGCTGTGGAGACCTGCCTGGGCTTCGTGCAGCTTTGCCACCCCATGAGCCTGGAGGACT AGGAAGCCCAGGGGGCCTAGGAGTGGGCCCTTCAGCTTGGGATGCTTGCCCCCCTGCTCTCCGTGGACTC CACCATGACCTTCTACTCCGAAAGATGTCATCATCATCCCCAGACCTGCTATCAGCAGCGCTGGGAGCCC GAGGCCGAGGGGCTACAGGGGGAGCTCGGGATCCTGGCTCACCACCTCCACCCCAGGGCGATACTCCTCC AAGTGAGGGATCAGCTCCTGGTTCCACCAGCCCAGATTCGCCTGGGGGAGCTAAAGGGGAACCCCCTCCA CCAGTAGGGCCTGGAGAAGGTGTGGGGCTGCTGGGAACTGGAAGGGAAGGGACTGCAGGCCGGGGAGGAA ACCGGGCTGGGTCCCAGCACTTGACCCCCGCTGCGCTGCTGTACAGGGCTGCTGTGACTCGAAGTCAGAA ACGTGGTATCTCATCTGAAGAAGAAGAAGGAGAGGTAGACAGTGAAGTAGAGCTACCCCCAAGTCAAAGG TGGCCTCAGGGCCCGAACATGCGTCAGTCACTATCTACATTCAGCTCAGAGAACCCATCAGATGTGGAGG AAGGTACAGCTAGTGAGCCTTCCCCAAGTGGCACACCAGAAGTTGGCAGTACCAACACTGATGAGCGACC AGATGAACGATCTGATGACATGTGCTCACAGGGCTCAGAAATTCCACTGGACCTACCTACTTCAGAGGTG GTCCCTGAACGTGAAGCCAGCTCCTTGCCCATGCAACACCAGGATGGCCAGGGCCCCAATCCTGAAGACT CAGACTGTGACAGCACTGAATTGGACAACTCCAACAGCATTGATGCCTTGCGGCCCCCAGCCTCCCTTCC TCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217856 representing NM_001163643
Red=Cloning site Green=Tags(s) MACLHETRTPSPSFGGFVSTLSEASMRKLDPDTSDCTPEKDLTPTQCVLRDVVPLGGQGGGGPSPSPGGE PPPEPFANSVLQLHEQDTGGPGGATGSPESRASRVRADEVRLQCQSGSGFLEGLFGCLRPVWTMIGKAYS TEHKQQQEDLWEVPFEEILDLQWVGSGAQGAVFLGRFHGEEVAVKKVRDLKETDIKHLRKLKHPNIITFK GVCTQAPCYCILMEFCAQGQLYEVLRAGRPVTPSLLVDWSMGIAGGMNYLHLHKIIHRDLKSPNMLITYD DVVKISDFGTSKELSDKSTKMSFAGTVAWMAPEVIRNEPVSEKVDIWSFGVVLWELLTGEIPYKDVDSSA IIWGVGSNSLHLPVPSSCPDGFKILLRQCWNSKPRNRPSFRQILLHLDIASADVLSTPQETYFKSQAEWR EEVKLHFEKIKSEGTCLHRLEEELVMRRREELRHALDIREHYERKLERANNLYMELNALMLQLELKEREL LRREQALERRCPGLLKSHPSRGLLHGNTMEKLIKKRNVPQKLSPHSKRPDILKTESLLPKLDAALSGVGL PGCPKGPPSPGRSRRGKTRHRKASAKGSCGDLPGLRAALPPHEPGGLGSPGGLGVGPSAWDACPPALRGL HHDLLLRKMSSSSPDLLSAALGARGRGATGGARDPGSPPPPQGDTPPSEGSAPGSTSPDSPGGAKGEPPP PVGPGEGVGLLGTGREGTAGRGGNRAGSQHLTPAALLYRAAVTRSQKRGISSEEEEGEVDSEVELPPSQR WPQGPNMRQSLSTFSSENPSDVEEGTASEPSPSGTPEVGSTNTDERPDERSDDMCSQGSEIPLDLPTSEV VPEREASSLPMQHQDGQGPNPEDSDCDSTELDNSNSIDALRPPASLPP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163643 |
| ORF Size | 2664 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163643.1, NP_001157115.1 |
| RefSeq Size | 5528 bp |
| RefSeq ORF | 2667 bp |
| Locus ID | 26404 |
| UniProt ID | Q60700 |
| Gene Summary | Protein kinase which is part of a non-canonical MAPK signaling pathway (PubMed:7983011, PubMed:8663324, PubMed:28111074). Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed:28111074). May be an activator of the JNK/SAPK pathway.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR217856 | Map3k12 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 12 (Map3k12), transcript variant 1 |
CNY 9,752.00 |
|
| MR217856L3 | Lenti ORF clone of Map3k12 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 12 (Map3k12), transcript variant 1 |
CNY 10,740.00 |
|
| MR217856L4 | Lenti ORF clone of Map3k12 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase 12 (Map3k12), transcript variant 1 |
CNY 10,740.00 |
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