United States
Germany
Japan
United Kingdom
China
Sppl2a (NM_023220) Mouse Tagged ORF Clone
CAT＃: MG217620
- TrueORF®
Sppl2a (tGFP-tagged) - Mouse RIKEN cDNA 2010106G01 gene (2010106G01Rik), (10ug)
ORF Plasmid: DDK
"NM_023220" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2010106G01Rik; C130089K23Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217620 representing NM_023220
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCTGCACTCGCTGCACGCTCCCGCGGCTGCCCTGCTCTGGAGCTGCCTGCTCGGACTGGCAG CTGCTCAGGAAGCCATTCTGCATGCGTCTACAAATGGCGTGTCTTCACTTTCCAAGGACTACTGCATGTA TTATAACAACAACTGGACACGACTTCCAAGTTCCCTAGAAAATGCAACTTCTCTGAGCTTGATGAATCTG ACGGGCACAGCACTGTGCCACCTTTCTGATATTCCTCCTGATGGCATAAGGAACAAGGCAGTGGTGGTGC ACTGGGGACCCTGCCATTTTCTTGAAAAAGCCAGAATTGCACAGGAAGGTGGTGCTGCAGCATTGCTGAT TGCCAACAACAGTGTCCTAATTCCTTCCTCAAGGAACAAATCTACATTTCAAAATGTGACTGTACTAATT GCAGTTATAACCCAAAAAGACTTCAAAGACATGAAAGAGACTCTTGGAGATGACATTACCGTGAAAATGT ATTCCCCATCCTGGCCTAACTTTGACTATACTCTGGTGGTTATTTTTGTAATTGCTGTGTTCACTGTGGC CTTAGGAGGATACTGGAGTGGACTTATTGAATTGGAAAACATGAAGTCAGTGGAAGACGCAGAAGACAGA GAGACAAGAAAAAAGAAGGACGATTACTTAACATTCAGTCCTCTCACGGTTGTTGTGTTCGTGGTCATCT GCTGTATAATGATTGTCTTACTGTATTTCTTCTACAGGTGGCTTGTGTATGTTATGATAGCGATTTTCTG CATAGCATCGTCAATGAGCCTGTACAACTGTCTTTCTGCATTGATTCATAGGATGCCATGTGGACAGTGC ACGATTTTGTGTTGTGGCAAAAACATTAAAGTGAGCCTGATTTTCCTTTCCGGGCTGTGCATCTCAGTTG CTGTTGTGTGGGCTGTATTTCGAAATGAGGATAGGTGGGCTTGGATTTTACAGGATATCTTAGGAATTGC TTTCTGTCTCAACTTAATTAAAACAATGAAGTTACCCAATTTCATGTCATGCGTGATACTGCTAGGCCTT CTTCTCATCTACGATGTGTTCTTTGTCTTCATCACACCTTTCATCACAAAGAATGGTGAGAGCATCATGG TGGAACTTGCTGCTGGGCCTTTCGAGAATGCTGAAAAGTTGCCAGTAGTTATCAGGGTGCCAAAACTGAT GGGTTACTCAGTCATGAGTGTATGCTCCGTGCCTGTTTCAGTATTGGGTTTCGGAGATATCATTGTACCA GGCCTGTTGATTGCATATTGTAGAAGATTCGATGTTCAGACTGGTTCTTCTATATACTATATTTCATCCA CAATTGCCTATGCTGTTGGCATGATCATTACCTTTGTTGTCCTGATGGTGATGAAAACAGGGCAGCCTGC TCTCCTCTACTTGGTACCTTGTACACTTATTACTGTCTCAGTCGTTGCTTGGAGTCGTAAGGAAATGAAA AAGTTCTGGAAAGGCAGCAGCTATCAGGTGATGGACCACCTGGACTATTCAACAAATGAAGAAAATCCAG TGACGACTGATGAGCAGATTGTACAACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217620 representing NM_023220
Red=Cloning site Green=Tags(s) MGLLHSLHAPAAALLWSCLLGLAAAQEAILHASTNGVSSLSKDYCMYYNNNWTRLPSSLENATSLSLMNL TGTALCHLSDIPPDGIRNKAVVVHWGPCHFLEKARIAQEGGAAALLIANNSVLIPSSRNKSTFQNVTVLI AVITQKDFKDMKETLGDDITVKMYSPSWPNFDYTLVVIFVIAVFTVALGGYWSGLIELENMKSVEDAEDR ETRKKKDDYLTFSPLTVVVFVVICCIMIVLLYFFYRWLVYVMIAIFCIASSMSLYNCLSALIHRMPCGQC TILCCGKNIKVSLIFLSGLCISVAVVWAVFRNEDRWAWILQDILGIAFCLNLIKTMKLPNFMSCVILLGL LLIYDVFFVFITPFITKNGESIMVELAAGPFENAEKLPVVIRVPKLMGYSVMSVCSVPVSVLGFGDIIVP GLLIAYCRRFDVQTGSSIYYISSTIAYAVGMIITFVVLMVMKTGQPALLYLVPCTLITVSVVAWSRKEMK KFWKGSSYQVMDHLDYSTNEENPVTTDEQIVQQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023220 |
| ORF Size | 1569 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023220.2, NP_075709.2 |
| RefSeq Size | 5629 bp |
| RefSeq ORF | 1572 bp |
| Locus ID | 66552 |
| UniProt ID | Q9JJF9 |
| Gene Summary | Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217462 | Sppl2a (untagged) - Mouse RIKEN cDNA 2010106G01 gene (2010106G01Rik), (10ug) |
CNY 6,750.00 |
|
| MR217620 | Sppl2a (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010106G01 gene (2010106G01Rik) |
CNY 3,896.00 |
|
| MR217620L3 | Lenti ORF clone of Sppl2a (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010106G01 gene (2010106G01Rik) |
CNY 6,460.00 |
|
| MR217620L4 | Lenti ORF clone of Sppl2a (mGFP-tagged) - Mouse RIKEN cDNA 2010106G01 gene (2010106G01Rik) |
CNY 6,460.00 |
