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Fzd10 (NM_175284) Mouse Tagged ORF Clone
CAT＃: MG217595
- TrueORF®
Fzd10 (tGFP-tagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10), (10ug)
ORF Plasmid: DDK
"NM_175284" in other vectors (4)
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CNY 8,096.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Fz-10 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217595 representing NM_175284
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAACACCCGGGCCCGCGCCTGTGGTTGGTGCTGCAGGTGATGATAGGCTCGTGCACGGCCATCAGCT CCATGGACTTAGAGCGCCCTGGAGACGGCAAGTGCCAGCCGGTGGAGATTCCCATGTGCAAGGACATCGG CTACAACACCACCCGCATGCCCAACCTGATGGGTCACGAGAACCAGCGCGAGGCGGCCATCCAACTGCAC GAGTTCGCGCCGCTCGTGGAGTACGGCTGCCACAGCCACCTTCGCTTCTTCCTGTGTTCGCTGTACGCGC CCATGTGCACCGAGCAGGTCTCCACACCCATCCCTGCTTGCCGGGTCATGTGCGAGCAGGCCCGGCTCAA GTGCTCGCCGATCATGGAGCAGTTCAAATTCAGGTGGCCGGACTCCCTGGATTGCAGCAAGCTCCCCAAC AAGAACGACCCCAACTACCTGTGCATGGAGGCACCCAACAACGGCTCGGATGAGCCCAGCCGGGGCTCTG GCATGTTTCCTCCGCTCTTCAGGCCCCAGAGGCCCCACAGCGCGCAGGAGCACCCACTAAAGGACGGGGG TCCGGGGCGCGCAGGTTGTGACAACCCAGGCAAGTTCCACCATGTGGAGAAGAGCGAATCTTGCGCACCG CTTTGCACTCCGGGGGTGGATGTGTATTGGAGCCGCGACGACAAGCGCTTCGCTGTGGTCTGGCTGGCCA TCTGGTCCGTGCTGTGCTTCTTCTCCAGCGCCTTCACCGTGCTCACCTTCCTCATCGACCCATCGCGCTT CAGGTACCCCGAACGTCCTATCATCTTCCTCTCCATGTGCTACTGCGTTTATTCGGTGGGCTATATCATC CGCCTCTTCGCGGGCGCGGAGAGCATCGCTTGTGACCGGGACAGTGGGCAGCTGTATGTTATCCAGGAAG GACTGGAGAGCACGGGCTGTACCTTAGTCTTCTTGGTACTTTACTACTTCGGCATGGCCAGCTCTTTATG GTGGGTGGTCCTCACCCTCACTTGGTTCCTGGCTGCTGGAAAGAAGTGGGGCCATGAGGCCATTGAAGCC AACAGCAGCTACTTTCACCTGGCAGCTTGGGCCATCCCGGCTGTGAAGACTATCTTGATCTTGGTGATGC GCAGGGTGGCAGGGGATGAGCTCACTGGTGTGTGTTATGTGGGCAGCATGGATGTCAATGCTCTGACCGG CTTCGTGCTGGTCCCGCTGGCTTGCTACCTAGTCATCGGCACTTCCTTCATCCTGTCCGGCTTTGTGGCT TTATTCCACATCCGGAGGGTGATGAAAACGGGTGGGGAGAACACGGACAAGCTGGAGAAGCTCATGGTAC GCATAGGGGTCTTCTCCCTCCTCTACACTGTGCCGGCCACCTGTGTGATTGCCTGCTATTTTTATGAACG CCTCAACATGGACTACTGGAAGATGCTGGCCACCCAGCACAAGTGTAAGATGAACAATCAGACCAAGACA CCTGACTGCCTGATGACCACCTCCATCCCTGCCGTGGAGGTCTTCATGGTCAAAGTGTCCATGCTGCTGG TGGTGGGCATCACCAGTGGGGTGTGGGTCTGGACTTCCAAGACCCTGCAGTCCTGGCAACACGTATGCAG CCGGGGGCTAAAGAGAAAAAGCCGGAGGAAACCAGCCAGTGTGGTCACCAGTGCAGGGATCTACAAAAAA GCCCAGCACCCCCAAAAACCTCACCTTGGGAAGTATGAACTGCCGGCCCAGCCTTCAGCCTGCGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217595 representing NM_175284
Red=Cloning site Green=Tags(s) MQHPGPRLWLVLQVMIGSCTAISSMDLERPGDGKCQPVEIPMCKDIGYNTTRMPNLMGHENQREAAIQLH EFAPLVEYGCHSHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFKFRWPDSLDCSKLPN KNDPNYLCMEAPNNGSDEPSRGSGMFPPLFRPQRPHSAQEHPLKDGGPGRAGCDNPGKFHHVEKSESCAP LCTPGVDVYWSRDDKRFAVVWLAIWSVLCFFSSAFTVLTFLIDPSRFRYPERPIIFLSMCYCVYSVGYII RLFAGAESIACDRDSGQLYVIQEGLESTGCTLVFLVLYYFGMASSLWWVVLTLTWFLAAGKKWGHEAIEA NSSYFHLAAWAIPAVKTILILVMRRVAGDELTGVCYVGSMDVNALTGFVLVPLACYLVIGTSFILSGFVA LFHIRRVMKTGGENTDKLEKLMVRIGVFSLLYTVPATCVIACYFYERLNMDYWKMLATQHKCKMNNQTKT PDCLMTTSIPAVEVFMVKVSMLLVVGITSGVWVWTSKTLQSWQHVCSRGLKRKSRRKPASVVTSAGIYKK AQHPQKPHLGKYELPAQPSACV TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175284 |
| ORF Size | 1746 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175284.3, NP_780493.1 |
| RefSeq Size | 3005 bp |
| RefSeq ORF | 1749 bp |
| Locus ID | 93897 |
| UniProt ID | Q8BKG4 |
| Gene Summary | Receptor for Wnt proteins (PubMed:15923619). Functions in the canonical Wnt/beta-catenin signaling pathway (PubMed:15923619). The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219323 | Fzd10 (untagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10), (10ug) |
CNY 7,410.00 |
|
| MR217595 | Fzd10 (Myc-DDK-tagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10) |
CNY 6,496.00 |
|
| MR217595L3 | Lenti ORF clone of Fzd10 (Myc-DDK-tagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10) |
CNY 7,700.00 |
|
| MR217595L4 | Lenti ORF clone of Fzd10 (mGFP-tagged) - Mouse frizzled homolog 10 (Drosophila) (Fzd10) |
CNY 7,700.00 |
