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Lrrc8d (NM_178701) Mouse Tagged ORF Clone
CAT＃: MG217464
- TrueORF®
Lrrc8d (tGFP-tagged) - Mouse leucine rich repeat containing 8D (Lrrc8d) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_178701" in other vectors (4)
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CNY 9,410.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810473G09Rik; 4930525N13Rik; A930019F03; Lrrc5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217464 representing NM_178701
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTACCCTTGCGGAAGTTGCTTCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAGCCATGGT GGGACGTGTTTATGGATTACCTGGCAGTCGTTATGCTGATGGTAGCCATCTTTGCAGGGACCATGCAACT TACCAAAGATCAGGTGGTCTGTTTGCCAGTGTTGCCGTCGCCTGCAAATTCAAAAGCGCACACACCACCC GGAAATGCTGACGTCACCACCGAAGTCCCCAGGATGGAAACAGCCACACACCAAGACCAAAACGGGCAGA CGACAACGAATGACGTTGCCTTTGGCACATCCGCTGTGACCCCTGACATACCTCTCCAAGCCACCCATCC TCATGCAGAGTCCACCCTTCCCAATCAGGAGGTGAAGAAGGAGAAGAGAGACCCAACGGGCCGAAAAACC AACTTGGATTTTCAGCAGTACGTATTTATCAATCAGATGTGTTACCATCTGGCCCTCCCTTGGTACTCCA AGTACTTTCCATACCTTGCCCTTATACACACCATCATCCTTATGGTCAGTAGCAACTTTTGGTTCAAATA TCCCAAAACGTGCTCCAAGGTTGAGCATTTTGTTTCAATATTAGGAAAGTGTTTCGAATCTCCCTGGACA ACTAAGGCGCTGTCAGAGACGGCCTGTGAAGACTCTGAAGAGAACAAGCAGCGGATAACGGGCGCCCAGA CCCTGCCGAAGCATGTGTCCACCAGCAGCGATGAGGGGAGCCCCAGCGCCAGCACCCCCATGATCAACAA AACTGGCTTCAAGTTCTCTGCTGAGAAACCGGTGATCGAAGTTCCCAGCATGACAATCCTGGACAAGAAA GACGGGGAGCAGGCCAAAGCCCTGTTTGAGAAAGTAAGGAAATTCCGTGCCCACGTGGAAGACAGTGACT TGATCTATAAGCTCTATGTGGTCCAAACGCTCATCAAAACTGCCAAGTTCATTTTTATCCTCTGCTACAC TGCGAACTTTGTCAACGCCATCAGCTTCGAGCACGTCTGCAAGCCAAAAGTCGAGCACCTCACGGGGTAC GAGGTGTTCGAGTGCACTCACAATATGGCCTACATGTTGAAAAAGCTGCTCATCAGCTACATCTCCATCA TCTGCGTCTACGGCTTTATCTGCCTCTACACTCTCTTCTGGCTATTCAGGATCCCTCTGAAGGAGTACTC GTTTGAGAAAGTCCGGGAGGAGAGCAGCTTCAGCGACATCCCGGATGTCAAGAACGACTTTGCGTTCCTT CTGCACATGGTCGACCAGTACGACCAGCTCTATTCCAAGCGTTTCGGTGTGTTCTTATCCGAAGTCAGTG AGAACAAACTGAGGGAAATCAGCCTGAACCATGAGTGGACTTTCGAGAAACTCAGGCAGCACGTGTCCCG CAATGCCCAGGACAAGCAGGAGCTGCACCTCTTCATGCTGTCCGGGGTGCCCGACGCTGTCTTTGACCTC ACAGACCTGGATGTGCTAAAACTTGAACTGATCCCCGAAGCAAAAATTCCTGCCAAGATCTCTCAGATGA CTAACCTCCAAGAGCTCCACCTCTGCCACTGCCCTGCAAAGGTGGAACAGACTGCCTTTAGCTTCCTCCG AGATCACTTGAGATGCCTTCACGTGAAGTTCACGGATGTGGCTGAAATCCCCGCCTGGGTGTATCTGCTT AAAAACCTGCGGGAGCTGTACTTGATCGGCAATTTGAACTCGGAAAACAATAAGATGATCGGCCTTGAGT CGCTGCGAGAGCTGCGGCACCTTAAGATCCTCCACGTGAAGAGTAATCTAACCAAAGTCCCCTCCAACAT CACCGACGTGGCTCCGCACCTCACGAAGCTCGTCATCCACAACGACGGGACCAAGCTCTTGGTCCTGAAC AGCCTGAAGAAGATGATGAACGTGGCCGAGCTCGAGCTGCAGAACTGTGAGCTGGAGAGGATCCCACACG CCATCTTCAGCCTCTCTAATCTGCAGGAGCTGGATTTAAAGTCAAACAACATCCGGACGATCGAGGAGAT CATCAGCTTCCAACATCTGAAGCGACTGACTTGCTTAAAGCTGTGGCACAATAAAATTGTGGCCATCCCA CCCTCCATTACCCATGTCAAGAACCTGGAGTCCCTCTACTTCTCCAACAACAAGCTCGAGTCTTTACCGA CGGCAGTGTTTAGTTTACAGAAACTCAGATGCTTAGACGTCAGCTATAACAACATTTCCACGATCCCCAT AGAGATAGGTTTGCTCCAGAACCTGCAGCATTTGCACATCACAGGGAACAAGGTGGACATTCTGCCAAAA CAGTTGTTTAAGTGCGTGAAGTTGAGGACTTTGAACCTGGGGCAGAACTGTATCGCCTCCCTGCCTGAGA AAATCAGTCAGCTCACCCAGCTCACTCAGCTGGAGCTGAAGGGCAACTGCCTAGACCGCCTGCCAGCCCA GCTGGGCCAGTGTCGGATGCTCAAGAAGAGCGGGCTTGTTGTAGAAGACCAACTGTTTGACACGCTGCCA CTAGAAGTCAAAGAGGCATTGAATCAAGATGTCAATGTCCCCTTTGCAAACGGGATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217464 representing NM_178701
Red=Cloning site Green=Tags(s) MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPANSKAHTPP GNADVTTEVPRMETATHQDQNGQTTTNDVAFGTSAVTPDIPLQATHPHAESTLPNQEVKKEKRDPTGRKT NLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWT TKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKK DGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTLIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGY EVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFL LHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDL TDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLL KNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLN SLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVAIP PSITHVKNLESLYFSNNKLESLPTAVFSLQKLRCLDVSYNNISTIPIEIGLLQNLQHLHITGNKVDILPK QLFKCVKLRTLNLGQNCIASLPEKISQLTQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDQLFDTLP LEVKEALNQDVNVPFANGI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178701 |
| ORF Size | 2577 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178701.2 |
| RefSeq Size | 3920 bp |
| RefSeq ORF | 2580 bp |
| Locus ID | 231549 |
| UniProt ID | Q8BGR2 |
| Gene Summary | Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress. Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210975 | Lrrc8d (tGFP-tagged) - Mouse leucine rich repeat containing 8D (Lrrc8d) |
CNY 9,410.00 |
|
| MR217464 | Lrrc8d (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8D (Lrrc8d), transcript variant 2 |
CNY 8,550.00 |
|
| MR217464L3 | Lenti ORF clone of Lrrc8d (Myc-DDK-tagged) - Mouse leucine rich repeat containing 8D (Lrrc8d), transcript variant 2 |
CNY 10,450.00 |
|
| MR217464L4 | Lenti ORF clone of Lrrc8d (mGFP-tagged) - Mouse leucine rich repeat containing 8D (Lrrc8d), transcript variant 2 |
CNY 10,450.00 |
