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Pm20d1 (NM_178079) Mouse Tagged ORF Clone
CAT＃: MG216980
- TrueORF®
Pm20d1 (tGFP-tagged) - Mouse peptidase M20 domain containing 1 (Pm20d1), (10ug)
ORF Plasmid: DDK
"NM_178079" in other vectors (4)
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CNY 4,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4732466D17Rik; AI098026 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216980 representing NM_178079
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGCTACTTGCTAGCTTGCCCGCCTGGGCAGCTGTGCTCCTTCTCTTTTTCGCTACGGTCTCCG GATCCACTGGCCCTAGAAGCAGGGAAAATCGGGGGGCGTCCCGGATCCCTTCCCAGTTCAGCGAGGAGGA GCGTGTCGCTATAAAAGAGGCGCTGAAAGGTGCCATCCAGATTCCCACAGTGTCTTTCAGCCACGAGGAA TCCAACACCACAGCCCTTGCTGAGTTTGGAGAATATATCCGCAAAGCCTTCCCTACAGTGTTCCACAGCA GCCTTGTCCAACATGAAGTCGTGGCAAAGTATAGCCACCTGTTCACCATCCAAGGCTCAGACCCCAGTTT GCAGCCCTACATGCTGATGGCTCACATTGATGTGGTTCCTGCCCCGGAAGAAGGATGGGAGGTGCCCCCG TTCTCAGGCCTGGAACGCAATGGCTTCATCTATGGCCGGGGTGCGCTGGACAACAAAAACTCTGTGATGG CGATCCTGCATGCTTTGGAGCTCCTGTTGATCAGAAACTACAGCCCCAAAAGATCTTTCTTCATTGCTTT GGGCCATGATGAGGAGGTGTCCGGGGAAAAGGGGGCTCAGAAGATCTCAGCACTCTTACAGGCAAGGGGT GTCCAGCTAGCCTTCCTTGTGGATGAAGGGAGCTTTATCTTGGAAGGCTTCATTCCAAACCTCGAGAAGC CAGTTGCCATGATTTCAGTCACTGAGAAGGGTGCCCTTGACCTCATGCTGCAAGTAAACATGACTCCAGG CCACTCTTCAGCTCCCCCAAAGGAGACAAGCATTGGCATTCTTTCTGCCGCTGTCAGCCGACTGGAGCAG ACACCAATGCCGAATATGTTTGGAGGAGGGCCATTGAAGAAGACAATGAAGCTACTGGCAAATGAGTTTT CCTTCCCTATCAATATAGTCTTGAGAAACCTGTGGCTATTTCATCCCATTGTGAGCAGGATAATGGAGAG GAACCCCATAACAAATGCGCTGGTCCGAACTACCACAGCCCTCACCATGTTCAATGCAGGAATCAAGGTG AATGTCATCCCTCCATTGGCTCAGGCTACAATCAACTGCCGAATTCACCCTTCGCAGACAGTACATGAGG TCCTAGAACTTGTCAAGAACACCGTGGCTGATGACAGAGTCCAGCTGCATGTGTTGAGATCCTTTGAACC CCTGCCCATCAGCCCCTCTGATGACCAGGCCATGGGCTACCAGCTGCTTCAAGAGACCATACGATCTGTC TTCCCGGAAGTCGACATCGTCGTCCCCGGTATTTGTATTGCCAATACGGACACCCGACACTATGCCAACA TCACCAATGGCATGTACCGGTTCAACCCCCTTCCCCTGAACCCTCAGGACTTCAGTGGTGTCCATGGAAT CAATGAGAAAGTTTCCGTTCAGAACTACCAGAACCAGGTGAAGTTCATCTTTGAGTTCATCCAAAATGCC GACACTTACAAAGAGCCAGTTCCTCATCTGCATGAACTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216980 representing NM_178079
Red=Cloning site Green=Tags(s) MAELLASLPAWAAVLLLFFATVSGSTGPRSRENRGASRIPSQFSEEERVAIKEALKGAIQIPTVSFSHEE SNTTALAEFGEYIRKAFPTVFHSSLVQHEVVAKYSHLFTIQGSDPSLQPYMLMAHIDVVPAPEEGWEVPP FSGLERNGFIYGRGALDNKNSVMAILHALELLLIRNYSPKRSFFIALGHDEEVSGEKGAQKISALLQARG VQLAFLVDEGSFILEGFIPNLEKPVAMISVTEKGALDLMLQVNMTPGHSSAPPKETSIGILSAAVSRLEQ TPMPNMFGGGPLKKTMKLLANEFSFPINIVLRNLWLFHPIVSRIMERNPITNALVRTTTALTMFNAGIKV NVIPPLAQATINCRIHPSQTVHEVLELVKNTVADDRVQLHVLRSFEPLPISPSDDQAMGYQLLQETIRSV FPEVDIVVPGICIANTDTRHYANITNGMYRFNPLPLNPQDFSGVHGINEKVSVQNYQNQVKFIFEFIQNA DTYKEPVPHLHEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178079 |
| ORF Size | 1509 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178079.3, NP_835180.2 |
| RefSeq Size | 3540 bp |
| RefSeq ORF | 1512 bp |
| Locus ID | 212933 |
| UniProt ID | Q8C165 |
| Gene Summary | Bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. Thereby, this secreted protein may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217050 | Pm20d1 (untagged) - Mouse peptidase M20 domain containing 1 (Pm20d1), (10ug) |
CNY 6,460.00 |
|
| MR216980 | Pm20d1 (Myc-DDK-tagged) - Mouse peptidase M20 domain containing 1 (Pm20d1) |
CNY 3,752.00 |
|
| MR216980L3 | Lenti ORF clone of Pm20d1 (Myc-DDK-tagged) - Mouse peptidase M20 domain containing 1 (Pm20d1) |
CNY 6,270.00 |
|
| MR216980L4 | Lenti ORF clone of Pm20d1 (mGFP-tagged) - Mouse peptidase M20 domain containing 1 (Pm20d1) |
CNY 6,270.00 |
