Pde1a (NM_001159582) Mouse Tagged ORF Clone
CAT#: MG216159
- TrueORF®
Pde1a (tGFP-tagged) - Mouse phosphodiesterase 1A calmodulin-dependent (Pde1a) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001159582" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI987702; AW125737 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216159 representing NM_001159582
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCTACTGATACGGACATCGAAGAATTGGAAAACGCCACTTACAAGTATCTCATTGGAGAACAGA CCGAGAAGATGTGGCAGCGCCTGAAGGGAATACTAAGATGCTTAGTGAAGCAGCTGGAGAAAGGTGATGT CAACGTTGTTGACCTAAAGAAGAATATCGAATATGCAGCATCAGTACTGGAAGCTGTTTACATCGATGAG ACAAGAAGACTGCTGGACACAGAGGATGAACTCAGCGACATCCAGACTGACTCCGTCCCATCGGAAGTCC GGGATTGGTTGGCTTCAACTTTCACACGCAAAATGGGGATGATGAAAAAGAAGCCTGAAGAAAAACCAAA GTTTCGCAGCATTGTCCATGCTGTCCAGGCTGGGATTTTTGTGGAAAGGATGTACAGGAAGAACTATCAC ATGGTTGGTTTGACATATCCTGCAGCTGTCATAGTGACACTCAAGGAAGTTGATAAATGGTCTTTTGATG TATTTGCCTTAAATGAAGCAAGCGGGGAGCATAGCCTGAAGTTTATGATTTATGAACTCTTTACCAGATA TGATCTTATCAACCGCTTCAAGATTCCTGTTTCTTGCCTAATTGCCTTTGCAGAAGCCTTAGAAGTTGGT TACAGCAAGCACAAAAATCCATATCATAATTTGGTCCATGCAGCTGACGTCACTCAAACTGTGCATTATA TAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTTTAGCCATGGTCTTTGCAGCTGC CATTCATGATTATGAGCACACAGGAACAACAAACAACTTCCATATTCAGACGAGGTCGGACGTTGCTATT CTGTACAACGACCGCTCAGTGCTTGAGAATCACCACGTCAGCGCAGCCTACAGACTTATGCAAGAGGAGG AAATGAATATTTTGGTAAATTTGTCTAAAGATGACTGGAGGGATCTTCGGAACTTAGTGATTGAAATGGT CTTAGCGACAGACATGTCAGGGCATTTTCAGCAAATTAAAAACATAAGAAACAGCTTGCAGCAGCCGGAA GGGATTGACAGAGCCAAAACTATGTCCCTGATACTCCATGCAGCTGACATCAGCCACCCAGCCAAAACTT GGAAGTTGCACTACAGATGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAGGGAGACAAAGAAGCTGA ATTAGGGCTTCCATTTTCTCCACTTTGTGATCGGAAGTCAACAATGGTTGCCCAGTCGCAAATAGGTTTC ATTGATTTCATAGTGGAGCCAACGTTTTCTCTCCTGACGGACTCAACAGAGAAAATTGTCATTCCTCTTA TAGAGGAAGCTTCAAAGTCACAGTCTTCAAACTACGGGGCAAGCAGCTCGTCCACCATGATTGGGTTCCA TGTTGCTGACTCTCTGAGGCGGTCAAATACAAAAGGCTCCGTGTGTGATGGGAGCTATGCCCCAGACTAC TCGCTCTCAGCTGTGGATCTGAAGAGTTTCAAAAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGA GGTGGAAAGAGTTGGCTGCCCAAGGTGAACTTGATCTTCATAAGAACTCAGAAGAGTTAGGAAATACTGA AGAAAAGCATGCTGACACACGTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216159 representing NM_001159582
Red=Cloning site Green=Tags(s) MGSTDTDIEELENATYKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNVVDLKKNIEYAASVLEAVYIDE TRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMMKKKPEEKPKFRSIVHAVQAGIFVERMYRKNYH MVGLTYPAAVIVTLKEVDKWSFDVFALNEASGEHSLKFMIYELFTRYDLINRFKIPVSCLIAFAEALEVG YSKHKNPYHNLVHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAI LYNDRSVLENHHVSAAYRLMQEEEMNILVNLSKDDWRDLRNLVIEMVLATDMSGHFQQIKNIRNSLQQPE GIDRAKTMSLILHAADISHPAKTWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGF IDFIVEPTFSLLTDSTEKIVIPLIEEASKSQSSNYGASSSSTMIGFHVADSLRRSNTKGSVCDGSYAPDY SLSAVDLKSFKNNLVDIIQQNKERWKELAAQGELDLHKNSEELGNTEEKHADTRP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001159582 |
ORF Size | 1635 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159582.1, NP_001153054.1 |
RefSeq Size | 4514 bp |
RefSeq ORF | 1638 bp |
Locus ID | 18573 |
UniProt ID | Q61481 |
Gene Summary | Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218815 | Pde1a (untagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1, (10ug) |
CNY 7,030.00 |
|
MR216159 | Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1 |
CNY 4,056.00 |
|
MR216159L3 | Lenti ORF clone of Pde1a (Myc-DDK-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1 |
CNY 6,460.00 |
|
MR216159L4 | Lenti ORF clone of Pde1a (mGFP-tagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 1 |
CNY 6,460.00 |