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Ap4e1 (NM_175550) Mouse Tagged ORF Clone
CAT＃: MG216130
- TrueORF®
Ap4e1 (tGFP-tagged) - Mouse adaptor-related protein complex AP-4 epsilon 1 (Ap4e1), (10ug)
ORF Plasmid: DDK
"NM_175550" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310033A20Rik; 9930028M04Rik; AV087807 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216130 representing NM_175550
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGACATGGTGGAGAGGACGCTGACCGCACTGCCGGGGCTCTTCCTGCAGAACCAGCTGGGAGGAC CTGCGGCCTCCAGAGCACCCTTCTTCTCCAGGCTGGGCGGCCTCATCCGTGGCGTCACTGCACTCTCATC CAAGCACGAAGAAGAGAAATTAATCCAGCAGGAACTGAGTAGTCTGAAGGCAACTGTTTCTGCTCCGACC ACGACACTGAAAACGATGAAGGAATGTATGGTGAGACTTATATACTGTGAGATGCTTGGATACGATGCTT CTTTTGGTTATATACATGCAATCAAGTTGGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTGGGTTATTT GGCTGTTTCTTTATTTCTACATGAAAGTCATGAACTTTTGCTTCTCCTTGTGAATACAGTGGTAAAGGAC CTGCAGAGCACTAATCTGGTAGAAGTATGTATGGCACTTACTGTTGTCAGCCAGATTTTCCCTCGAGAAA TGATTCCAGCCGTTCTTCCACTAATAGAAGATAAACTTCAACATTCTAAGGAGATTATACGAAGAAAAGC AGTTCTGGCATTATACAAATTTTACCTCATTGCCCCCAATCAAGTACAGCACATCCACACTAAATTTCGG AAAGCGCTTTGTGACAGAGATGTTGGGGTCATGGCTGCCTCTTTGCACATATACCTTAGGATGATTAAGG AAAATGCTTCTGGCTATAAAGACTTGACGGAGAGTTTTGTAACAATTTTAAAGCAGGTGGTGGGCGGGAA GCTCCCGGTAGAGTTCAGTTACCACAGCGTGCCAGCACCATGGTTACAGATTCAGCTCCTGAGAATACTG GGACTGCTGGGGAAAGACGATGAAAGGACAAGTGAATTAATGTATGATGTTCTTGATGAATCCTTACGAA GAGCCGAGTTAAATCACAATGTCACTTACGCTATTCTGTTTGAATGTGTACACACAATCTATTCTATTTA TCCTAAGTCAGAATTACTTGAGAAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAAC CTCAAATATTTAGGACTGAAGGCTCTTACCTATGTTATCCAGCAGGATCCTTCTCTGGCTCTTCAACACC AGATAACCATAATTGAATGCCTAGACCACCCTGATCCCATTATTAAAAGAGAGACTCTGGAACTTCTTTA CAGAATTACAAATGCACAGAATGTAGTGGTTATTGTACAGAAAATGCTTGAATACTTACATCAGAGCAAA GAAGAGCATATCATCATCAGTTTAGTTGGCAGAATTGCTGAGCTGGCTGAGAAATATGCTCCTGACAACG TGTGGTTTATTCAGACAATGAATGCTGTGTTTTCAGTGGGAGGAGATGTAATGCATCCAGATATTCTCAG CAACTTCCTGAGACTGCTAGCAGAAGGTTTTGATGATGAAACAGAAGATCAACAATTAAGACTCTACGCA GTTCAGTCTTATCTTACTTTACTAGATATGGAAAATACTTTCTATCCACAGAGATTTCTTCAAGTTATGA GTTGGGTGTTAGGAGAGTACTCCTACCTCTTAGATAAAGAAAGTCCAGAGGAGGTTATAACCAGGCTCTA CAAGTTACTTATGAGTGACTCCATTTCTTCAGAAACGAAAGCATGGTTGTTTGCTGCGGTAACCAAGTTG ACACCTCAAGCTCATTCTTCTCCCTTAGTTGAGAAATTAATCCAAGAATTCACTGTATCCTTGAATACTT GTCTGAGACAGCATGCATTTGAATTGAAACATCTGCATGAAAATACAGAGCTTATGAAGAGCCTGCTTCA GGGTGCTCAGAATTGTGAAGACATAGTGGCAGATGCGTCTTTATCTTTTCTGGATGGTTTTGTGGCTGAA GGACTCAGTCAGGGAGCAGCACCTTATAAGCCCCATCACCAGCGCCAAGAGGAACAGCTCTCTCAGGAAA AGGTTCTCAATTTTGAACCATATGGACTATCATTTTCTTCATCTGGCTTCACTGGACGACAGTCTCCGGC TGGCATTTCCCTGGGCTCTGATATATCTGGAAACAGTGCCGAGACAGGGCTGAAAGAGACAAGCAGTTTG AAGATGGAAGGGATAAAGAAATTATGGGGTAAAGAGGGCTATCTGCCCAAGAAGGAGAGTGGAACTGGTG ATAAACCGGAAGCCTCACATGTTCCTGCAGAGGGTGCAACAGTGGAGAATGTAGACCAAGCCACGACCAG AAAGGACCAAGCTCAAGGCCATATCCCTTCTACAGAGGAGAAAGAAAAGCAGCTGTTGGCATCATCGCTG TTTGTTGGGCTAGGACCAGAAAATACAGTCGATTTGTTGGGAAAAGCAGATGTTGTGTCTCACAAGTTCA GGAGGAAATCAAAACTCAAGGTAGCCCAAAGCGACAAGACACCCAGCGCTCCTACTGCTCCCTGCTCCGC CCTCAGCCTTGGCTCAGATGTGGCTGGTGGGGATGAGGACGGTCTGAGTGCTGTGGATAGAGGGGACGGA GAACTCAGTTCAGAGCTTTTTCGTTCTGAGTCTCTCTCGGGGCCGCCCTCAGCTGAGAAGCTCGAGAGTG TCAGCTTGCCTGTGCCTTCTTTATTTGCTGATAACAACATGGAAGTCTTTAATCCCCCTTCATCCTCTGC AACTTCAACTGTCAAGGAAGAAACTCCTGAGTGCAGGCATTCAGGTCTCGTGGAAATCTGCAGTAATGAA GCCGTGTCTGTGTCTTCTTACAAAGTCTGGAGGGATGATTGTTTATTGGTGATCTGGGCAGTCACTAGTA AAACTGACTCAGAGTTCACAGATGCTCAGTTAGAAATTTTTCCTGTGGAAAATTTCAAGATCATTGAGCA ACCTGAATGTTCTTCTCCTGTGATAGAAACAGAACGCACCAAATCCTTTCAATACAGTGTGCAGATGGAA AGCCCTTGTATAGAGGGGACTCTCTCTGGTTTTATAAAGTATCAAATGATGGATACGCATTCTGTTCAAC TGGAATTTTCCATGAACTTACCATTATTAGATTTTATTAGACCATTAAAGATCTCAACTGAAGACTTTGG CAAACTCTGGTTGTCCTTTGCAAATGATGTGAAGCAAACTATAAAAATATCAGAGCCTGGAGTTGCTCTG ACTTCTGTCCTAACAGAGCTGCAGCAGAACCTGAGACTTCGTGTGATTGACGTCATAGGCAATGAAGGGC TGTTGGCCTGTAAGCTGCTCCCATCCACCCCCTGTGTGCTGCACTGCCGAGTACATGCTGACGCGGTAGC TCTGTGGTTCCGGTCCTCTAGCTCTGTTCTTTCAGACTATTTATCGTGTCACTGTCAAAAAGTGATGCAG ACATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216130 representing NM_175550
Red=Cloning site Green=Tags(s) MSDMVERTLTALPGLFLQNQLGGPAASRAPFFSRLGGLIRGVTALSSKHEEEKLIQQELSSLKATVSAPT TTLKTMKECMVRLIYCEMLGYDASFGYIHAIKLAQQGNLLEKRVGYLAVSLFLHESHELLLLLVNTVVKD LQSTNLVEVCMALTVVSQIFPREMIPAVLPLIEDKLQHSKEIIRRKAVLALYKFYLIAPNQVQHIHTKFR KALCDRDVGVMAASLHIYLRMIKENASGYKDLTESFVTILKQVVGGKLPVEFSYHSVPAPWLQIQLLRIL GLLGKDDERTSELMYDVLDESLRRAELNHNVTYAILFECVHTIYSIYPKSELLEKAAKCIGKFVLSPKIN LKYLGLKALTYVIQQDPSLALQHQITIIECLDHPDPIIKRETLELLYRITNAQNVVVIVQKMLEYLHQSK EEHIIISLVGRIAELAEKYAPDNVWFIQTMNAVFSVGGDVMHPDILSNFLRLLAEGFDDETEDQQLRLYA VQSYLTLLDMENTFYPQRFLQVMSWVLGEYSYLLDKESPEEVITRLYKLLMSDSISSETKAWLFAAVTKL TPQAHSSPLVEKLIQEFTVSLNTCLRQHAFELKHLHENTELMKSLLQGAQNCEDIVADASLSFLDGFVAE GLSQGAAPYKPHHQRQEEQLSQEKVLNFEPYGLSFSSSGFTGRQSPAGISLGSDISGNSAETGLKETSSL KMEGIKKLWGKEGYLPKKESGTGDKPEASHVPAEGATVENVDQATTRKDQAQGHIPSTEEKEKQLLASSL FVGLGPENTVDLLGKADVVSHKFRRKSKLKVAQSDKTPSAPTAPCSALSLGSDVAGGDEDGLSAVDRGDG ELSSELFRSESLSGPPSAEKLESVSLPVPSLFADNNMEVFNPPSSSATSTVKEETPECRHSGLVEICSNE AVSVSSYKVWRDDCLLVIWAVTSKTDSEFTDAQLEIFPVENFKIIEQPECSSPVIETERTKSFQYSVQME SPCIEGTLSGFIKYQMMDTHSVQLEFSMNLPLLDFIRPLKISTEDFGKLWLSFANDVKQTIKISEPGVAL TSVLTELQQNLRLRVIDVIGNEGLLACKLLPSTPCVLHCRVHADAVALWFRSSSSVLSDYLSCHCQKVMQ TS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175550 |
| ORF Size | 3366 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175550.3, NP_780759.2 |
| RefSeq Size | 6524 bp |
| RefSeq ORF | 3369 bp |
| Locus ID | 108011 |
| UniProt ID | Q80V94 |
| Gene Summary | Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223659 | Ap4e1 (untagged) - Mouse adaptor-related protein complex AP-4, epsilon 1 (Ap4e1), (10ug) |
CNY 14,350.00 |
|
| MR216130 | Ap4e1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-4, epsilon 1 (Ap4e1) |
CNY 7,816.00 |
|
| MR216130L3 | Lenti ORF clone of Ap4e1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex AP-4, epsilon 1 (Ap4e1) |
CNY 10,260.00 |
|
| MR216130L4 | Lenti ORF clone of Ap4e1 (mGFP-tagged) - Mouse adaptor-related protein complex AP-4, epsilon 1 (Ap4e1) |
CNY 10,260.00 |
