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Jmjd7 (NM_001114637) Mouse Tagged ORF Clone
CAT＃: MG216128
- TrueORF®
Jmjd7 (tGFP-tagged) - Mouse jumonji domain containing 7 (Jmjd7), (10ug)
ORF Plasmid: DDK
"NM_001114637" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC106779 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216128 representing NM_001114637
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGGCTCTGGAGGCAGTTCGGAGGGCGTTGCAAGAGTTCCCGGCGGCGGCTCGCGACCTCA ATGTACCTCGTGTTGTGCCCTACCTGGATGAGCCCCCAAGCCCACTCTGCTTCTACCGGGATTGGGTGTG CCCCAACAGGCCCTGCATTATCCGAAATGCTCTGCAGCACTGGCCAGCCCTCCAGAAGTGGTCCCTGTCC TACTTAAGAGCCACGGTGGGCTCCACGGAGGTGAGTGTGGCTGTGACTCCAGATGGTTATGCGGACGCGG TGCGAGGGGACCGCTTTGTGATGCCTGCTGAACGCCGCCTGCCCATAAGCCATGTACTGGATGTGTTGGA AGGTCGGGCCCAGCACCCAGGAGTCCTCTATGTGCAGAAACAGTGTTCCAACCTGCCCACTGAGCTGCCC CAGCTTCTGTCTGACATTGAGTCCCATGTGCCCTGGGCCTCTGAGTCACTGGGGAAGATGCCTGATGCCG TGAACTTCTGGCTGGGTGATGCATCTGCAGTGACATCCTTGCACAAGGACCACTATGAGAATCTGTACTG TGTAGTCTCTGGCGAGAAACACTTCTTGTTACATCCACCCAGCGACCGGCCCTTCATCCCTTACAATCTC TACACACCAGCAACCTACCAGCTGACTGAAGAGGGCACTTTTAGGGTGGTGGACGAGGAAGCCATGGAGA AGGTACCCTGGATCCCACTGGACCCCTTGGCTCCAGACCTGACCCAGTACCCCAGTTACAGCCAGGCACA GGCCCTTCACTGCACAGTGCGGGCCGGCGAGATGCTGTACCTGCCAGCTCTGTGGTTCCACCATGTCCAG CAGTCCCACGGCTGTATTGCTGTGAATTTCTGGTATGACATGGAGTATGACCTCAAGTACAGTTACTTTC AACTGATGGACACCCTCACCAGGGCCACAGGCCTTGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216128 representing NM_001114637
Red=Cloning site Green=Tags(s) MAEAALEAVRRALQEFPAAARDLNVPRVVPYLDEPPSPLCFYRDWVCPNRPCIIRNALQHWPALQKWSLS YLRATVGSTEVSVAVTPDGYADAVRGDRFVMPAERRLPISHVLDVLEGRAQHPGVLYVQKQCSNLPTELP QLLSDIESHVPWASESLGKMPDAVNFWLGDASAVTSLHKDHYENLYCVVSGEKHFLLHPPSDRPFIPYNL YTPATYQLTEEGTFRVVDEEAMEKVPWIPLDPLAPDLTQYPSYSQAQALHCTVRAGEMLYLPALWFHHVQ QSHGCIAVNFWYDMEYDLKYSYFQLMDTLTRATGLD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001114637 |
| ORF Size | 948 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001114637.1, NP_001108109.1 |
| RefSeq Size | 1383 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 433466 |
| UniProt ID | P0C872 |
| Gene Summary | Bifunctional enzyme that acts both as an endopeptidase and 2-oxoglutarate-dependent monoxygenase (PubMed:28847961) (By similarity). Endopeptidase that cleaves histones N-terminal tails at the carboxyl side of methylated arginine or lysine residues, to generate 'tailless nucleosomes', which may trigger transcription elongation (PubMed:28847961). Preferentially recognizes and cleaves monomethylated and dimethylated arginine residues of histones H2, H3 and H4. After initial cleavage, continues to digest histones tails via its aminopeptidase activity (PubMed:28847961). Additionally, may play a role in protein biosynthesis by modifying the translation machinery. Acts as Fe(2+) and 2-oxoglutarate-dependent monoxygenase, catalyzing (S)-stereospecific hydroxylation at C-3 of 'Lys-22' of DRG1 and 'Lys-21' of DRG2 translation factors (TRAFAC), promoting their interaction with ribonucleic acids (RNA) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214968 | Jmjd7 (untagged) - Mouse jumonji domain containing 7 (Jmjd7), (10ug) |
CNY 3,990.00 |
|
| MR216128 | Jmjd7 (Myc-DDK-tagged) - Mouse jumonji domain containing 7 (Jmjd7) |
CNY 2,400.00 |
|
| MR216128L3 | Lenti ORF clone of Jmjd7 (Myc-DDK-tagged) - Mouse jumonji domain containing 7 (Jmjd7) |
CNY 4,750.00 |
|
| MR216128L4 | Lenti ORF clone of Jmjd7 (mGFP-tagged) - Mouse jumonji domain containing 7 (Jmjd7) |
CNY 4,750.00 |
