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Arhgap10 (NM_030113) Mouse Tagged ORF Clone
CAT＃: MG216047
- TrueORF®
Arhgap10 (tGFP-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10), (10ug)
ORF Plasmid: DDK
"NM_030113" in other vectors (4)
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CNY 8,650.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A930033B01Rik; PSGAP-m; PSGAP-s |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216047 representing NM_030113
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCTGCAGCCCCTGGAGTTTAGCGACTGCTACCTCGACAGCCCGTGGTTCCGGGAGAGGATCCGCG CGCACGAAGCGGAGCTCGAGAGGACCAACAAGTTCATCAAAGAGCTCATCAAAGACGGCAAGAACCTCAT CTCTGCCACCAAAAGTCTGTCAGCTGCCCAGAGGAAGTTCGCTCACTCGCTCAGAGACTTCAAGTTCGAG TTTATCGGCGATGCTGAGACTGACGATGAACGGTGCATAGATGCCTCCTTACGGGAATTTTCTAACTTTC TGAAGAATCTGGAAGAACAGAGAGAAATCATGGCATTAAGTGTGACTGAAACCCTGATTAAACCCTTGGA GAAATTCAGAAAAGAGCAGCTTGGAGCTGTAAAGGAAGAAAAAAAGAAGTTTGACAAAGAGACGGAAAAG AATTACAGTCTCATCGACAAGCACTTGACGCTGTCTGCCAGGAAGAAGGACTCACACTTGCAGGAGGCCG ACCTCCAAGTGGAGCAGAACCGGCAGCATTTCTACGAGCTCTCGCTTGAATATGTGTGCAAGCTCCAGGA AATCCAAGAAAGAAAGAAGTTCGAGTTTGTGGAGCCTATGCTGTCCTTCTTTCAGGGAATGTTCACTTTC TATCATCAAGGCCACGAGCTTTCCAAAGACTTCAATCACTACAAGATGGAACTACAAATCAACATTCAGA ATACACGGAATCGATTTGAGGGAACCAGATCAGAAGTGGAGGAACTTATGAATAAAATTAGACAAAATCC CAAGGACCAGAAGCGAGCCAGCCAGTTCACAGCAGAAGGCTACCTGTACGTCCAGGAGAAAAGGCCTGCT CCATTTGGGTCCAGTTGGGTCAAACACTACTGCATGTACCGGAAAACAGCCAAGAAATTCAACATGATTC CATTTGAGCACAGATCTGGGGGGAAACTGGGGGATGGCGAGGCTTTCTTTTTGAAAGAATGCACCAAAAG GCACATGGACTCCACGGACAGAAGGTTTTGCTTTGACATCGAAGCTGCTGACAGGCCTGGCGTCCCCTTG ACCGTGCAGGCGTTTTCAGAAGAAGAGAGGAAGCAGTGGTTGGAAGCTCTGGGTGGAAAAGAAGCTCTGT TCCACACGTTTAACAGAGCCATTGTCCCGAGACCGGAAGGAGGTGCACAGCTGGATAAGATGGGATTCAC AATTCTCAGAAAATGCATCAGCGCCGTTGAAACCCGAGGTATAAATGACCAAGGACTGTACAGAGTTGTG GGGGTGAGTTCAAAGGTCCAGAGACTTCTGAGTATGTTGATGGATGTAAAAATGTGCAATGAGCTGGACC TGGAGAATTCTGCAGATTGGGAAGTGAAGACAGTAACAAGTGCCTTGAAGCAGTATCTGAGGAGTCTTCC AGAGCCTCTCATGACCTATGAGTTACACAGAGACTTCATTGTTCCAGCCAAGAGCGGCAGCCCGGAATCC CGAGTTAATGCCATCCACTTCTTGGTACACAAACTGCCAGAGAAGAATAAGGAGATGTTGGATATCTTGG TGAAGCATCTAACCAACGTGTCAAGTCACTCGAAGCAGAATCTAATGACAGTGGCGAATTTAGGGGTGGT GTTTGGACCAACTCTGATGAGACCACAGGAAGAGACTGTTGCCGCCATCATGGATTTGAAGTTTCAGAAT ATCGTTGTAGAAATCTTAATTGAAAACCATGAAAAGATTTTTCGAACCTCGCCTGATACCACATTTGCTG AGCCCACATGCCTGTCAGCATCACCCCCAAACGCTCCACCACGACAGTCCAAGAGACAAGGGCAGAGGAC GAAGAGACCAGTGGCCGTGTACAACCTTTGTCTTGAGCTGGAGGAGGGTGACAGTCCCAGCCCTCTCAAG GAGGACCCCCCCAGCAGCAGTCAGGACTCTCTCTCCACCCCATCTCCCACAACGTCAGCTGCCCATGGGC CTCCTGGACTAGACGGAAACCACCTAGCAGCAGACGGAGGGAGCTGTGGAGATGCCACCGCCACCACGCC AAGCCAGACCCGGCCATCCATGGTGCAGTGGCTAAATATGCAGTCTCCAACCACACCAAGCTCCAACCCA GCTGGAACACCTCCTTCACCCAGAATGTCACCGTTCCCCCTCTCTCCTGCTGCCTCCATTGTAGACAAGC TGCCTGAGTGCGTTATCAATCGAAAGGCTCGGGCAGTGTACCCGTGTGAAGCAGAACACAGCTCGGAATT GTCATTTGAAATAGGAGCCATTTTTGAGGATGTTCAGACCTCAAGGGAACCCGGATGGCTGGAAGGGACC CTGAACGGCAAGCGAGGGCTGATTCCACAGAATTATGTCAAGCTCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216047 representing NM_030113
Red=Cloning site Green=Tags(s) MGLQPLEFSDCYLDSPWFRERIRAHEAELERTNKFIKELIKDGKNLISATKSLSAAQRKFAHSLRDFKFE FIGDAETDDERCIDASLREFSNFLKNLEEQREIMALSVTETLIKPLEKFRKEQLGAVKEEKKKFDKETEK NYSLIDKHLTLSARKKDSHLQEADLQVEQNRQHFYELSLEYVCKLQEIQERKKFEFVEPMLSFFQGMFTF YHQGHELSKDFNHYKMELQINIQNTRNRFEGTRSEVEELMNKIRQNPKDQKRASQFTAEGYLYVQEKRPA PFGSSWVKHYCMYRKTAKKFNMIPFEHRSGGKLGDGEAFFLKECTKRHMDSTDRRFCFDIEAADRPGVPL TVQAFSEEERKQWLEALGGKEALFHTFNRAIVPRPEGGAQLDKMGFTILRKCISAVETRGINDQGLYRVV GVSSKVQRLLSMLMDVKMCNELDLENSADWEVKTVTSALKQYLRSLPEPLMTYELHRDFIVPAKSGSPES RVNAIHFLVHKLPEKNKEMLDILVKHLTNVSSHSKQNLMTVANLGVVFGPTLMRPQEETVAAIMDLKFQN IVVEILIENHEKIFRTSPDTTFAEPTCLSASPPNAPPRQSKRQGQRTKRPVAVYNLCLELEEGDSPSPLK EDPPSSSQDSLSTPSPTTSAAHGPPGLDGNHLAADGGSCGDATATTPSQTRPSMVQWLNMQSPTTPSSNP AGTPPSPRMSPFPLSPAASIVDKLPECVINRKARAVYPCEAEHSSELSFEIGAIFEDVQTSREPGWLEGT LNGKRGLIPQNYVKLL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_030113 |
| ORF Size | 2358 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_030113.2, NP_084389.2 |
| RefSeq Size | 3038 bp |
| RefSeq ORF | 2361 bp |
| Locus ID | 78514 |
| UniProt ID | Q6Y5D8 |
| Gene Summary | GTPase activator for the small GTPases RhoA and Cdc42 by converting them to an inactive GDP-bound state. Essential for PTKB2 regulation of cytoskeletal organization via Rho family GTPases. Inhibits PAK2 proteolytic fragment PAK-2p34 kinase activity and changes its localization from the nucleus to the perinuclear region. Stabilizes PAK-2p34 thereby increasing stimulation of cell death.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221582 | Arhgap10 (untagged) - Mouse Rho GTPase activating protein 10 (Arhgap10), (10ug) |
CNY 10,070.00 |
|
| MR216047 | Arhgap10 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10) |
CNY 7,890.00 |
|
| MR216047L3 | Lenti ORF clone of Arhgap10 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10) |
CNY 9,790.00 |
|
| MR216047L4 | Lenti ORF clone of Arhgap10 (mGFP-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10) |
CNY 9,790.00 |
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