Cse1l (NM_023565) Mouse Tagged ORF Clone
CAT#: MG215858
- TrueORF®
Cse1l (tGFP-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l), (10ug)
ORF Plasmid: DDK
"NM_023565" in other vectors (4)
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CNY 10,640.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2610100P18Rik; AA407533; Capts; Cas; Xpo2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215858 representing NM_023565
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTCAGCGATGCGAATTTACAGACACTAACAGAGTATTTAAAGAAAACCCTGGATCCTGATCCTG CCATTAGACGGCCAGCTGAGAAGTTTCTCGAATCCGTAGAAGGAAATCAGAATTACCCACTGTTGCTTTT AACATTATTGGAAAAGTCACAGGATAATGTCATTAAAGTATGTGCTTCAGTAACCTTCAAGAACTATATT AAAAGGAATTGGAGAATTGTTGAAGATGAACCAAACAAAATTTGCGAAGCTGATCGAGTAGCTATTAAAG CCAACATAGTGCATCTGATGCTGAGCAGTCCAGAGCAGATTCAGAAGCAGCTGAGTGATGCTATTAGCAT CATTGGCAGAGAAGATTTCCCTCAGAAATGGCCTGACTTGTTGACAGAAATGGTGAATCGCTTTCAGAGT GGAGATTTTCACGTCATTAATGGAGTCCTTCGTACAGCGCACTCACTATTTAAGAGATACCGGCATGAGT TCAAGTCAAATGAGCTGTGGACTGAAATTAAACTTGTCTTGGATGCCTTTGCTTTGCCTTTGACTAATCT ATTCAAGGCCACCATTGAACTGTGCAGTACCCATGCAAATGACGCCTCTGCCCTGAGGATTCTTTTTTCT TCCTTGATTTTGATCTCAAAATTGTTCTATAGTTTGAACTTTCAGGATCTCCCTGAATTCTTTGAAGATA ATATGGAAACTTGGATGAACAATTTTCATACCCTCCTGACACTGGATAATAAGCTCTTGCAGACTGATGA TGAAGAGGAAGCAGGCTTACTGGAGCTTTTAAAGTCTCAGATCTGTGATAATGCCGCTCTCTATGCACAG AAATATGACGAAGAGTTTCAGCGGTACCTCCCTCGCTTTGTCACAGCTATCTGGAACTTACTCGTTACAA CAGGGCGAGAAGTTAAATATGACTTGTTGGTTAGCAATGCAATTCAGTTTCTGGCTTCAGTTTGTGAAAG GCCACATTATAAGAATCTGTTTGAGGACCAGAACACGCTGACAAGTATCTGTGAAAAAGTTATTGTGCCT AACATGGAATTTAGAGCTGCTGATGAAGAAGCTTTTGAAGATAATTCTGAGGAGTACATACGGAGGGATT TGGAAGGCTCTGATATTGATACTAGACGCAGGGCTGCTTGTGATCTTGTACGAGGATTATGCAAATTTTT TGAAGGTCCTGTGACAGGAATCTTCTCTGGTTATGTTAATTCCATGCTCCAGGAGTATGCAAAAAACCCG TCTGTCAACTGGAAACACAAAGATGCAGCTATTTACCTTGTCACATCTTTGGCATCAAAAGCCCAGACAC AGAAGCATGGAATCACGCAAGCCAATGAACTTGTAAACCTAACTGAATTTTTCGTGAATCACATACTTCC TGATTTAAAATCAAATAATGTGAATGAGTTTCCTGTGCTTAAAGCTGATGGTATCAAATATATTATGATT TTTAGGAATCAAGTACCAAAAGAGCACCTCCTAGTCTCTATTCCCCTCTTGATCAGTCACCTGGAGGCTG AGAGTATTGTCGTCCACACTTATGCCGCACATGCTCTCGAAAGGCTGTTCACTATGAGAGGGTCAAACAA TACCACTCTTTTTACAGCTGCAGAAATCGCACCGTTTGTTGAGATTCTGCTAACAAACCTTTTCAAAGCT CTCACACTTCCTGGCTCTTCAGAAAATGAATATATTATGAAAGCTATCATGAGAAGTTTCTCCCTCCTGC AAGAGGCCATCATCCCCTATATCCCCACTCTCATCACGCAGCTCACACAGAAGCTGCTGGCTGTTAGTAA GAATCCCAGTAAACCTCATTTCAATCACTACATGTTTGAAGCAATATGCTTGTCTATAAGAATAACCTGC AAAGCTAACCCTGCTGCTGTTGTGAATTTTGAGGAGGCTCTGTTTCTAGTGTTCACTGAGATCTTACAAA ATGATGTACAAGAGTTTATCCCATATGTCTTTCAAGTGATGTCTTTACTTCTGGAAACACATAAAAATGA TATCCCGTCTTCCTATATGGCCTTGTTTCCCCACCTCCTTCAGCCAGTACTGTGGGAAAGGACAGGAAAC ATCCCTGCTCTAGTGAGGCTGCTCCAGGCATTCTTAGAACGAGGCTCAAGTACAATAGCAACTGCTGCAG CTGACAAAATTCCTGGATTACTGGGTGTCTTCCAGAAGCTGATTGCATCCAAGGCAAACGATCACCAAGG TTTCTATCTTCTCAACAGTATAATCGAACACATGCCTCCTGAATCAGTTGACCAGTATAGGAAGCAGATC TTCATTCTACTATTCCAGAGACTGCAAAACTCCAAGACCACCAAGTTTATCAAGAGTTTCTTAGTGTTTA TTAACTTATATTGCATTAAGTATGGGGCACTAGCATTACAAGAAATATTTGATGGCATACAACCAAAAAT GTTTGGAATGGTTTTGGAAAAAATCATTATTCCAGAGATTCAAAAGGTATCTGGGAACGTGGAGAAAAAA ATCTGTGCAGTTGGCATAACCAAATTACTCACAGAGTGTCCACCAATGATGGACACGGAGTACACTAAGC TGTGGACTCCTTTGTTACAATCTCTCATTGGCCTGTTTGAATTACCTGAAGATGACAGCATTCCTGATGA AGAGCATTTCATTGACATAGAAGATACACCAGGATACCAGACTGCCTTCTCACAGCTGGCATTTGCTGGT AAAAAGGAACATGATCCTGTAGGTCAGATGGTAAACAACCCCAAAATCCATCTGGCACAGTCACTTCACA AACTGTCAACTGCTTGTCCTGGAAGGGTTCCCTCGATGGTGAGCACCAGCCTCAATGCAGAAGCCTTGCA GTATCTCCAAGGTTACCTGCAGGCAGCCAGTGTGACCCTGTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215858 representing NM_023565
Red=Cloning site Green=Tags(s) MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYI KRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQS GDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFS SLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQ KYDEEFQRYLPRFVTAIWNLLVTTGREVKYDLLVSNAIQFLASVCERPHYKNLFEDQNTLTSICEKVIVP NMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFSGYVNSMLQEYAKNP SVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSNNVNEFPVLKADGIKYIMI FRNQVPKEHLLVSIPLLISHLEAESIVVHTYAAHALERLFTMRGSNNTTLFTAAEIAPFVEILLTNLFKA LTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITC KANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGN IPALVRLLQAFLERGSSTIATAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQI FILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKK ICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDSIPDEEHFIDIEDTPGYQTAFSQLAFAG KKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023565 |
ORF Size | 2913 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023565.3, NP_076054.1 |
RefSeq Size | 3604 bp |
RefSeq ORF | 2916 bp |
Locus ID | 110750 |
UniProt ID | Q9ERK4 |
Gene Summary | Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222918 | Cse1l (untagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l), (10ug) |
CNY 12,540.00 |
|
MR215858 | Cse1l (Myc-DDK-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l) |
CNY 9,690.00 |
|
MR215858L3 | Lenti ORF clone of Cse1l (Myc-DDK-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l) |
CNY 11,590.00 |
|
MR215858L4 | Lenti ORF clone of Cse1l (mGFP-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l) |
CNY 11,590.00 |