Arhgap21 (NM_001128084) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5530401C11Rik; AA416458; ARHGAP10
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG215805 representing NM_001128084 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGCGGCCTCTG CCTGTGAGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCC ATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGA CACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAG GAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCC GGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAG ACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGG ATGAAGACATTCTCCAAGTGCTACAATTTACAAAGGATGTTACAGCACTGGCATATTCTCAAGATGCATA CCTGAAAGGCAATGAAGCTTACAGCGGCAATGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCC TGGTTGCCATCGACTCCTTCAGCTACTGCACAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACA AACAGCAAACCAGTGCACCAGTCCTCACACAACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCC GTCACCAACAGATGTTGCAAAGTCAAACACAGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATT GTGCCTTCTGAGAAGGTGGTAGACTTGCTAGCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTG AAGAGGTAAGGTATGGTGTAAATGAGCAGGCCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATC AGTTCCCACTGCCCATCTGATTCATCAGACCACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTT AAATCTGGAAATTACAGTGGACATTCAGAAGGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTC CTGTGTCTGTCAATCACTACTCTGCAAACTCCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAA AGAATACATTGATAATAGGAGATTGCACATAGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGA GCTGCATCTCAGAGCGCAGCAGACTATAACCAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTC GAAGCACCTCTCATGACCGAGTGCCCCAGTCTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACT GGAAGATTCTGTGCTGATGAAGTATTGCCCAAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTT AGCTTCAATAATCATAGAACTCGTTCGTGGGATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCA ATTCTGAAAGTCAGATACCTGACTCAAATGGGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGA GCAGGATGATAGACGCGGTATTCACGAAAGACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCA AATTTGACTGTGGCTCCCGTTGTTAATTCTGATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCT CACAGTTTAAAAAAATCCCGCCGGACCTAAGACCACCACATTCCAACAGAAATTTTCCAACCACGACTGG GGTGTCACTTCAACGGGGTATTGCACAAGACAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAA GTGCCACCTCCACCTGTGTCAAAACCGTCCTTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGC CTGTCAATCATCTGCATCAACACAGTGTGCTGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGA GCACCAGCTGGAGACTGAGGTATCCTCGTGTCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGT GAGAATTTGGGAACATCAGACTTAGAACTACCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGG CTGAAATTCAGCAGCCAGATGTGCTAGATAATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGG CCGCCAGACGCCACAGCCTTTAAGACATCAGTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCA GACACCACCTGCTGGCTGCCAAACGATGCACGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAG CTTCAAGCACCAGCCCTCCTGGTGATTCCCTGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAG CATCGACCACGAGATTGCACATATTCCTGCATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCC ATTGCAACTGTTCCCCCCAGCCTCACGACCTCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAG AATCTGTTGGACCTCCCAGCCTGGATGGCCAGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGA AGGACTGGATGATTATAGAGAAGATGCAAAACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAG ATTACAGACAGCCAGAAGTCATCAGAAGACTCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTG ATGCAGCCCGAGAAGGCTGGCTCCAGTTCCGACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAG TATTCGGCCATGGAAACAGATGTATGTTGTCCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGA GAGCAGACAACGCCATCAGAAGAAGAGCAGCCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACA GTGAGACCAAGAGGCGGAATGTATTCCGACTTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGA CAGAGATGATATGTTATCATGGATCAAGACTATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGA GTTACCAACAGAGATCTAATTAGTCGAAGAATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGT TGCCAAAAACACCTCGCCAGAGTCTTAGCATCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGAC TCAAAGCCCACATTCTCCAAAGGAGGAGTCCGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCCCCT AAAGACAAAGGTACTTGGAGAAGAGGCATTCCAAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGGCCG CCACAGGGACCTTTGGAGTTAGGCTGGATGACTGCCCACCCGCCCACACCAACAGGTACATTCCATTAAT CGTTGACATATGTTGCAAATTAGTTGAAGAAAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCCGGC AACAACGCAGCCATTTCAAGCATGCAGGAAGAACTCAACAAGGGGATGGCAGACATTGACATACAAGATG ATAAATGGCGAGATTTGAATGTGATAAGCAGTTTACTAAAATCCTTCTTCAGAAAGCTCCCTGAGCCTCT CTTTACGAATGACAAATATGCTGACTTCATTGAGGCCAATCGCAAAGAGGACCCACTGGATCGGCTACGA ACATTAAAGAGACTAATTCATGACCTGCCTGAGCATCATTTTGAGACGCTCAAGTTTCTCTCGGCTCATC TGAAGACAGTGGCAGAGAACTCAGAAAAGAATAAGATGGAACCCAGAAACCTAGCGATCGTTTTTGGTCC AACTCTAGTGAGAACATCAGAAGACAACATGACCCACATGGTCACGCACATGCCTGACCAGTATAAGATC GTAGAGACTCTCATCCAGCATCATGACTGGTTTTTCACAGAAGAAGGTGCCGAAGAGCCTCTTACAGCAG TGCAGGAGGAGAACACAGTAGACTCCCAGCCAGTACCAAACATAGATCATTTGCTCACCAACATTGGAAG GACAGGCGTGCTCCCTGGAGATGTATCAGATTCAGCTACTAGTGACTCCGCAAAATCTAAGGGCTCCTGG GGATCCGGGAAGGATCAGTATAGCAGGGAACTGCTTGTATCCTCCATCTTCGCAGCTGCCAGTCGCAAGA GGAAAAAGCCGAAAGAGAAAGCCCAGCCCAGCAGCTCTGAGGATGAGCTGGACAGTGTGTTCTTTAAGAA GGAGAATACGGAACAGAGTCACAGTGAGATTAAAGAAGAGTCCAAAAGAGAAAGCGAGACATCAGGTAGC AAGCAGAGGGTCGTCGTTGCCAAAGAAAGTAACACTAAGAAAGACTCGGGTACAACGAAGGAGGAGAAGA AGATACCGTGGGAAGAACCATCCCCACCACACAGCTCCAAACGCAACAGGTCACCAACCCTCAGCTGTCG CCTGGCCATGCTGAAAGAGGGCCCCAGATCACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGGTCT GACTCAGGCACTCTGCTTAGCACGTCTTCCCAGGCTTCGCTCTTGAGGTCCTCCACTAAAAAATCCACCA GTCCGGAAACCAAGCACAGTGAGTTTTTGTCCATTGCTGGTACCACCACCTCGGATTATTCTACCACATC ATCCACTACCTACTTGACCAGCCTGGACTCCAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCCGAGAGC AAGGGGGATGAGGCTGATGACGAGAGGAGCGAGCTGGTGAGTGAAGGACGGCCAGTGGAGACCGACAGTG AGAGTGAGTTCCCCGTGTTTCCCGCCACCCTGACTTCAGACCGGCTGTTCCGAGGAAAATTCCAGGATGT TGCCAGGGTGAGCCGCAGGAACTCCGAAGGCAGTGAGGCCAGCTGCACCGAGGGAAGCCTCACACCGAGT TTAGACAGCCGCAGGCAGCAGTTCAGCTCCCATAGACTGATCGAATGCGACACACTGTCCAGGAAGAAGT CTGCTCGCTTCAAGTCGGACAGTGGGAGCCCCGGAGACACCAGGACTGAGAAAGAAACTCCTGCCCTGGC CAAGATGTTTGACGTCATGAAGAAAGGCAAGTCGACAGGGAGCCTCCTCACACCCAGCAGAAGTGAATCC GAAAAGCAGGAGGCCACTTGGAAAACAAAAATAGCAGATCGTTTAAAACTGAGACCCAGGGCACCCGCAG ACGACATGTTTGGAGTTGGGAATCAAAAACCTACTGCTGAGACGGCGAAAAGGAAAAACATCAAACGCAG ACACACGCTTGGAGGCCACAGAGATGCTACTGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCATGAGCAG AGTGCAGACAAAGAGTCTGAACTTTCGGCTGTGAATCGGTTAAAACCCAAATGCTCAGCCCAGGACCTTT CCATCTCAGACTGGCTCGCCAGGGAGCGCGTCCGCACCAGTGCCTCCGACCTTAGCAGAGGCGAAGGCCT CGAGCCCCAGGCTGAGAGCCCCAGCGTGCTGGGAACACCCATCAGCACCCACTCACCACCGTCTCAGCAG CCCGAAGCAAGGGTTGCTGCTACCAGCACCTTGGCTTCAACCAGCCAGTCCCCTCTTTTCACACCACCAC AGTCACCTGACCAAATAAACAGAGAAAGCTTCCAGAACATGAGCCAAAATGCTAGTTCTACAGCTAACAT CCACCCCCACAAACAGTCTGAAAGTCCAGACACTAAAGCAGAGACTCCTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG215805 representing NM_001128084 Red=Cloning site Green=Tags(s) MATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLR HFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGK TYSQVIALIQNSDTTLELSVMPKDEDILQVLQFTKDVTALAYSQDAYLKGNEAYSGNARNIPEPPPVCYP WLPSTPSATAQPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVI VPSEKVVDLLANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILL KSGNYSGHSEGISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLR AASQSAADYNQVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPV SFNNHRTRSWDYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGS NLTVAPVVNSDNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLK VPPPPVSKPSFSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLS ENLGTSDLELPAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGS DTTCWLPNDARREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPS IATVPPSLTTSAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIK ITDSQKSSEDSGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRR EQTTPSEEEQPISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTG VTNRDLISRRIKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPP KDKGTWRRGIPSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPG NNAAISSMQEELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLR TLKRLIHDLPEHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKI VETLIQHHDWFFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSW GSGKDQYSRELLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGS KQRVVVAKESNTKKDSGTTKEEKKIPWEEPSPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGS DSGTLLSTSSQASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAES KGDEADDERSELVSEGRPVETDSESEFPVFPATLTSDRLFRGKFQDVARVSRRNSEGSEASCTEGSLTPS LDSRRQQFSSHRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSES EKQEATWKTKIADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQ SADKESELSAVNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQ PEARVAATSTLASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001128084
ORF Size	5865 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001128084.2, NP_001121556.2
RefSeq Size	6974 bp
RefSeq ORF	5868 bp
Locus ID	71435
Gene Summary	Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions (By similarity).[UniProtKB/Swiss-Prot Function]

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