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Akap8 (NM_019774) Mouse Tagged ORF Clone
CAT＃: MG215718
- TrueORF®
Akap8 (tGFP-tagged) - Mouse A kinase (PRKA) anchor protein 8 (Akap8), (10ug)
ORF Plasmid: DDK
"NM_019774" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200016A02Rik; AA673585; AKA; AKAP-8; AKAP95; AU015639 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215718 representing NM_019774
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAAGGCTACGGAGGCTATGGGGCATGGAGTGCTGGACCTGCCAACACCCAGGGTACATATGGAA GTGGTATGACCAGCTGGCAAGGTTATGAAAACTACAACTACTACAATGCCCAGAACACCAGTGTCCCTGC AGGAACACCCTATAGTTACGGCCCAGCCTCATGGGAGGCCACCAAGACCAATGATGGTGGCCTGGCAGCT GGGAGCCCTGCCATGCATGTGGCGTCTTTTGCCCCAGAGCCATGTACCGACAACTCAGACTCGCTCATTG CCAAGATCAATCAACGTTTGGACATGTTGTCTAAGGAAGGAGGCAGGGGTGGGATCAGCAGCGGTGGGGA GGGCGTGCAGGACCGAGACAGCTCCTTCCGCTTCCAGCCATACGAGTCCTACGACGCCAGGCCCTGTATA CCTGAGCATAATCCCTACCGCCCCGGCTACGGTTATGATTATGACTTTGACTTGGGAACTGACCGCAATG GTAGCTTTGGTGGGACATTCAACGACTGTCGGGACCCAGCCCCAGAGCGGGGCTCCCTTGATGGTTTCTT GAGGGGCCGGGGCCAGGGCCGCTTCCAAGACCGGAGCAACTCCAGCACCTTCATACGTAGTGACCCTTTC ATGCCACCCTCAGCCTCGGAGCCCTTATCCACTACGTGGAATGAGCTGAACTACATGGGTGGCCGTGGTC TCGGTGGGCCCTCCACCAGCAGGCCACCTCCTTCCCTCTTCTCCCAGTCCATGGCCCCTGACTACAGCAT GATGGGCATGCAGGGGGTGGGCGGTTTTGGTGGTACCATGCCTTACGGATGTGGCCGGTCCCAGACGCGG ATACGGGATTGGCCTAGAAGGAGGGGGTTTGAACGCTTTGGACCAGACAACATGGGCAGGAAGCGGAAGC AGTTTCCATTGTATGAAGAACCTGATGCCAAACTGGCCCGTGCTGACAGTGACGGAGATCTGTCTGAAAA CGATGATGGAGCTGGTGACTTAAGATCAGGAGATGAAGAATTCAGGGGGGAGGATGACTTGTGTGACTCC AGGAAGCAGAGAGGAGAAAAGGAGGACGAGGATGAGGATGTGAAGAAGAGACGGGAGAAACAAAGGAGGA GAGACAGGATGCGGGACCGAGCAGCTGACAGGATTCAGTTTGCCTGTTCTGTATGCAAGTTTCGTAGCTT TGAAGATGAAGAAATTCAAAAGCATCTGCAAAGTAAATTTCACAAAGAGACTTTGCGATTTATAAGTACC AAACTGCCCGACAAGACAGTAGAATTCCTCCAGGAGTACATCATAAACAGGAATAAGAAAATTGAGAAAC GGCGTCAGGAGTTGTTGGAGAAGGAGAGCCCTAAACCCAAACCAGATCCATTCAAAGGCATTGGCCAGGA GCATTTCTTCAAAAAGATTGAAGCCGCACATTGCCTAGCCTGTGACATGCTGATTCCTGCGCAGCATCAG CTTCTCCAGCGGCATCTGCATTCCGTGGACCATAACCATAATCGAAGGTTGGCTGCTGAGCAGTTCAAGA AAACAAGTCTCCATGTGGCTAAGAGTGTTTTGAACAACAAACATATAGTGAAGATGCTGGAAAAATACCT CAAGGGTGAGGATCCTTTTGTCAATGAAACTGCTGACCTTGAGACAGAAGGAGATGAAAATGTAGGAGAG GAGAAGGAGGAGACACCAGAGGAGGTAGCTGCCGAAGTCTTAGCAGAGGTGATCACAGCAGCAGTGAAGG CTGTAGAGGGGGAAGGAGAACCTGCTGCAGCGCATAGTGACGTCCTAACTGAAGTGGAAGGGCCTGTGGA CACAGCAGAGGCCAGCAGTGACCCCCACACTGAAAAGCTACTAGAAGAGCAGACCTGTGAAGCAGCATCT GAAACCAGGAGCATCGAAGACAAGACCAGAGGTGAGGCTGCTGAGGCCAGAAATGAAGCAGCTATGCCAA CAGCAGACGCAGGAAGCACGTTACCTGTCATAGCAATCCCGGGAATCATGGAAGATGAGCTGGAACAAAC TGGTGCAGAGGCCAAAGATATTCCCACCGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215718 representing NM_019774
Red=Cloning site Green=Tags(s) MEQGYGGYGAWSAGPANTQGTYGSGMTSWQGYENYNYYNAQNTSVPAGTPYSYGPASWEATKTNDGGLAA GSPAMHVASFAPEPCTDNSDSLIAKINQRLDMLSKEGGRGGISSGGEGVQDRDSSFRFQPYESYDARPCI PEHNPYRPGYGYDYDFDLGTDRNGSFGGTFNDCRDPAPERGSLDGFLRGRGQGRFQDRSNSSTFIRSDPF MPPSASEPLSTTWNELNYMGGRGLGGPSTSRPPPSLFSQSMAPDYSMMGMQGVGGFGGTMPYGCGRSQTR IRDWPRRRGFERFGPDNMGRKRKQFPLYEEPDAKLARADSDGDLSENDDGAGDLRSGDEEFRGEDDLCDS RKQRGEKEDEDEDVKKRREKQRRRDRMRDRAADRIQFACSVCKFRSFEDEEIQKHLQSKFHKETLRFIST KLPDKTVEFLQEYIINRNKKIEKRRQELLEKESPKPKPDPFKGIGQEHFFKKIEAAHCLACDMLIPAQHQ LLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNKHIVKMLEKYLKGEDPFVNETADLETEGDENVGE EKEETPEEVAAEVLAEVITAAVKAVEGEGEPAAAHSDVLTEVEGPVDTAEASSDPHTEKLLEEQTCEAAS ETRSIEDKTRGEAAEARNEAAMPTADAGSTLPVIAIPGIMEDELEQTGAEAKDIPTE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019774 |
| ORF Size | 2061 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019774.4, NP_062748.2 |
| RefSeq Size | 3695 bp |
| RefSeq ORF | 2064 bp |
| Locus ID | 56399 |
| UniProt ID | Q9DBR0 |
| Gene Summary | This gene encodes a member of the A-kinase anchoring protein (AKAP) family. These proteins are characterized by their ability to bind to the R subunit of protein kinase A (PKA) and anchor the protein at different subcellular locations. This protein has been shown to regulate apoptosis and to be involved in palatogenesis. Knockdown of this gene has been associated with altered histone modifications and reduced expression of developmental genes in mouse embryonic stem cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220508 | Akap8 (untagged) - Mouse A kinase (PRKA) anchor protein 8 (Akap8), (10ug) |
CNY 8,840.00 |
|
| MR215718 | Akap8 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 8 (Akap8) |
CNY 5,040.00 |
|
| MR215718L3 | Lenti ORF clone of Akap8 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 8 (Akap8) |
CNY 7,700.00 |
|
| MR215718L4 | Lenti ORF clone of Akap8 (mGFP-tagged) - Mouse A kinase (PRKA) anchor protein 8 (Akap8) |
CNY 7,700.00 |
