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Scn3a (NM_018732) Mouse Tagged ORF Clone
CAT＃: MG215436
- TrueORF®
Scn3a (tGFP-tagged) - Mouse sodium channel voltage-gated type III alpha (Scn3a), (10ug)
ORF Plasmid: DDK
"NM_018732" in other vectors (2)
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CNY 21,280.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Gm1000; Nav1.3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215436 representing NM_018732
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGGCACTGCTGGTACCCCCGGGACCTGAGAGCTTCCGCCTTTTCACTCGAGAATCTCTTGCTG CTATCGAAAAGCGTGCTGCAGAAGAGAAAGCCAAGAAACCCAAGAAAGAACAAGACATTGACGATGAGAA CAAGCCAAAGCCAAACAGTGACTTGGAAGCTGGGAAGAACCTTCCATTTATCTATGGAGACATTCCTCCA GAGATGGTGTCGGAGCCTCTGGAGGACCTGGACCCCTACTACGTCAGTAAGAAAACTTTTGTAGTGTTGA ATAAAGGGAAGGCAATTTTTCGATTCAGTGCCACCTCCGCCTTGTATATTTTAACTCCACTAAACCCCGT TAGGAAAATTGCTATTAAGATTTTGGTACACTCTTTATTCAGCATGCTTATCATGTGCACTATTTTGACC AACTGTGTATTTATGACATTGAGCAATCCTCCTGACTGGACGAAGAATGTAGAGTACACATTCACTGGGA TCTATACCTTTGAGTCACTTATAAAGATCTTGGCCAGAGGATTCTGCTTAGAAGATTTCACATTTCTTCG TGACCCATGGAACTGGCTGGATTTCAGTGTCATCGTGATGGCATATGTGACAGAGTTTGTGGACCTGGGC AATGTCTCAGCGCTGAGAACGTTCAGAGTTCTCCGAGCATTGAAAACAATATCAGTCATTCCAGGTTTAA AGACCATCGTGGGGGCCCTGATCCAGTCGGTGAAGAAGCTGTCTGACGTCATGATACTCACTGTGTTCTG TCTGAGCGTCTTTGCTCTCATCGGGCTGCAGCTCTTCATGGGCAACCTGAGGAATAAATGCTTGCAGTGG CCTCCAAGCGATTCTGCTTTTGAGATCAACACTACTTCCTACTTCAATGGCACAATGGACTCAAATGGGA CATTTGTTAATGTAACAATGAGCACATTCAACTGGAAGGACTATATCGCAGATGACAGCCACTTTTATGT TTTGGATGGACAAAAAGATCCTTTACTTTGTGGAAATGGGTCCGATGCAGGACAATGTCCAGAAGGGTAC ATCTGTGTGAAGGCTGGACGAAACCCCAACTACGGTTACACGAGCTTTGACACATTTAGCTGGGCCTTCT TATCGCTGTTTCGACTCATGACTCAAGACTACTGGGAGAACCTTTACCAGTTGACATTACGTGCAGCTGG GAAAACCTACATGATCTTTTTCGTCCTGGTAATTTTCTTGGGCTCATTTTATTTGGTGAACTTGATCCTG GCTGTGGTGGCCATGGCCTATGAGGAACAAAATCAGGCCACACTGGAGGAGGCTGAGCAGAAAGAGGCGG AGTTTCAGCAGATGTTGGAGCAGTTGAAAAAGCAGCAAGAGGAGGCTCAGGCGGTGGCAGCTGCCTCAGC AGCGTCCAGAGACTTCAGTGGGATAGGAGGGTTAGGAGAACTTCTGGAGAGTTCTTCAGAAGCTTCCAAG TTGAGCTCCAAGAGTGCCAAGGAGTGGAGGAATCGGAGGAAGAAGAGGAGACAGAGGGAGCACTTGGAGG GAAACCACAGACCCGAAGGAGACAGGTTTCCCAAGTCGGAATCAGAAGACAGCGTCAAGCGAAGGAGTTT CCTGTTCTCCCTGGATGGGAACCCGCTGAGCGGCGACAAGAAGCTGTGCTCTCCCCATCAGTCTCTCTTG AGTATCCGTGGCTCCCTGTTTTCCCCAAGACGCAATAGCAAAACGAGCATTTTCAGCTTCAGAGGTCGGG CGAAGGACGTGGGGTCTGAGAATGACTTTGCGGATGATGAACACAGCACCTTTGAAGATAGCGAGAGCAG GAGAGACTCACTGTTTGTGCCGCACAGACCTGGAGAGCGACGCAACAGTAACGGTACCACCACTGAAACA GAAGTCAGGAAGAGAAGGCTAAGTTCTTACCAGATCTCGATGGAAATGCTGGAGGATTCCTCTGGGAGAC AAAGAGCCATGAGCATAGCCAGTATCCTGACCAACACGATGGAGGAACTTGAAGAATCTAGACAGAAGTG TCCACCATGCTGGTATAGATTTGCCAATGTGTTTTTGATCTGGGACTGTTGTGATTCATGGTTGAAAGTA AAGCATCTTGTGAATTTGATTGTGATGGATCCATTTGTTGACCTGGCCATCACCATCTGCATCGTGTTAA ACACACTGTTCATGGCCATGGAGCACTACCCGATGACGGAGCAGTTCAGCAGTGTGCTGACGGTGGGAAA CCTGGTCTTCACCGGGATCTTCACAGCCGAGATGGTCCTGAAAATCATCGCAATGGATCCCTATTACTAT TTCCAAGAGGGCTGGAATATCTTTGATGGAATTATTGTTAGCCTGAGTTTAATGGAGCTTGGCCTGGCAA ACGTGGAGGGGCTGTCCGTGCTTCGGTCCTTCAGACTGCTGCGAGTCTTCAAGTTGGCAAAATCCTGGCC CACACTGAATATGCTCATTAAGATCATCGGCAACTCGGTGGGCGCACTGGGCAACCTGACCCTGGTGCTG GCCATCATCGTCTTCATTTTTGCCGTGGTCGGCATGCAGCTGTTTGGAAAGAGCTACAAGGAGTGTGTTT GCAAGATCAATGAGGACTGCAAGCTCCCGCGCTGGCACATGAACGACTTCTTCCACTCCTTCCTGATAGT GTTCCGCGTGCTGTGTGGGGAGTGGATAGAGACCATGTGGGACTGCATGGAGGTCGCGGGCCAGACCATG TGCCTTATTGTGTTTATGTTGGTCATGGTGATTGGGAACCTTGTGGTTCTGAACCTCTTCCTGGCCTTAT TGTTGAGTTCCTTTAGTTCAGACAACCTTGCTGCTACGGACGATGATAACGAAATGAACAACCTCCAGAT CGCGGTGGGAAGGATGCAAAAGGGGATTGATTATGTGAAAAATAAGATACGGGAGTGCTTCCGAAAAGCG TTTTTTAGAAAGCCGAAAGTGATAGAAATCCACGAAGGGAACAAAATAGACAGCTGCATGTCCAATAACA CGGGCGTAGTTGAAATAAGCAAAGAGCTTAACTACCTTAAAGACGGTAACGGAACCACCAGTGGCGTGGG TACTGGAAGCAGTGTGGAGAAATACGTAATTGATGAAAATGACTACATGTCATTCATCAACAACCCCAGC CTCACCGTGACGGTGCCAATTGCCGTGGGAGAGTCTGACTTTGAAAATTTAAACACGGAAGAGTTTAGCA GTGAGTCAGAACTGGAAGAAAGCAAGGAGAAATTAAATGCAACCAGCTCTTCTGAAGGAAGCACAGTTGA CGTTGCTCCGCCCCGAGAAGGTGAACAAGCTGAAATTGAACCTGAGGAGGACCTTAAGCCAGAAGCTTGC TTTACTGAAGGATGCATTAAAAAATTTCCCTTCTGCCAAGTAAGTACGGAAGAAGGTAAAGGAAAAATCT GGTGGAATCTTAGGAAGACCTGCTATAGCATTGTGGAACACAACTGGTTTGAGACGTTCATTGTGTTCAT GATTCTCCTCAGTAGTGGTGCTTTGGCCTTTGAAGATATATACATTGAGCAACGGAAGACCATCAAAACC ATGCTGGAGTATGCTGACAAAGTCTTCACTTACATCTTCATCCTGGAAATGCTCCTCAAATGGGTGGCCT ATGGATTTCAAACCTATTTCACCAATGCCTGGTGCTGGTTGGACTTCTTGATTGTTGATGTTTCTTTGGT TAGCCTGGTGGCCAACGCTCTTGGCTATTCAGAACTTGGTGCCATCAAATCCCTACGGACCCTGAGAGCT CTGAGGCCGCTCCGAGCCTTATCCCGCTTTGAAGGCATGAGGGTGGTTGTGAACGCTCTTGTTGGTGCAA TCCCCTCCATCATGAATGTGCTACTGGTGTGCCTCATCTTCTGGTTAATCTTTAGTATCATGGGTGTGAA TCTGTTTGCTGGAAAGTTCTATCACTGTGTTAACATGACAACGGGCAGCATGTTCGACATGAGTGAAGTC AACAATTTCAGCGACTGTCAGGCTCTTGGCAAGCAAGCCCGATGGAAGAATGTGAAAGTCAACTTTGACA ATGTTGGGGCTGGCTACCTGGCATTGCTGCAAGTGGCCACATTCAAAGGCTGGATGGATATTATGTATGC AGCTGTGGATTCACGGGACGTCAAACTGCAGCCTGTATATGAAGAAAATCTGTACATGTATCTGTACTTT GTCATCTTCATCATCTTTGGGTCGTTCTTCACTCTAAATCTATTCATCGGCGTCATCATAGACAACTTCA ACCAGCAGAAGAAGAAGTTTGGAGGTCAAGACATCTTTATGACAGAAGAGCAGAAAAAGTACTACAATGC AATGAAGAAACTTGGCTCCAAAAAACCTCAGAAGCCCATCCCTCGACCTGCAAACAAATTTCAAGGAATG GTCTTTGACTTTGTAACCAGACAAGTGTTTGACATCAGCATCATGATCCTCATCTGCCTCAACATGGTGA CCATGATGGTGGAAACGGACGACCAGAGCAAATACATGACCCTGGTTTTGTCCCGAATCAACCTGGTATT CATCGTCCTCTTCACTGGGGAGTTTCTGCTGAAGCTCATCTCTCTCAGATACTACTACTTCACGATTGGC TGGAACATCTTTGACTTTGTGGTGGTGATTCTCTCAATTGTAGGAATGTTCCTTGCTGAGCTGATAGAGA AGTATTTTGTGTCTCCTACCCTGTTCCGAGTCATCCGCCTGGCCAGGATTGGACGAATCCTACGCCTGAT CAAAGGCGCCAAGGGGATCCGCACGCTGCTCTTTGCTCTGATGATGTCCCTTCCTGCGCTGTTCAACATC GGCCTCCTGCTTTTCCTCGTCATGTTCATCTACGCCATCTTTGGGATGTCCAACTTTGCCTATGTTAAAA AAGAGGCTGGAATTGATGACATGTTCAACTTTGAGACTTTTGGCAACAGCATGATCTGCCTGTTCCAAAT CACCACCTCTGCGGGCTGGGATGGACTGTTGGCCCCCATCCTCAACAGTGCACCTCCTGACTGTGACCCT GATGCAATTCACCCTGGAAGCTCAGTGAAGGGAGACTGTGGGAACCCATCTGTGGGGATTTTCTTTTTTG TCAGCTACATCATCATATCCTTCCTGGTTGTGGTGAACATGTACATTGCTGTCATCCTGGAGAACTTCAG CGTTGCCACAGAAGAAAGTGCAGAGCCCCTGAGTGAGGACGACTTTGAGATGTTCTACGAGGTCTGGGAG AAGTTCGACCCTGACGCCACCCAGTTCATAGAGTTCTGCAAGCTCTCTGACTTTGCAGCTGCCCTGGATC CTCCCCTCCTCATCGCAAAGCCAAACAAAGTCCAGCTCATTGCCATGGACCTGCCCATGGTGAGTGGAGA CCGCATCCACTGCCTGGACATCTTATTTGCTTTTACAAAGCGGGTCCTGGGTGAGAGTGGAGAGATGGAT GCCCTTCGAATCCAGATGGAAGATCGGTTCATGGCTTCCAATCCCTCCAAGGTCTCTTATGAGCCCATTA CCACCACTCTGAAGCGCAAACAAGAGGAGGTGTCTGCTGCTATCATTCAGCGTAATTACAGATGTTATCT TTTAAAGCAAAGGTTAAAAAACATATCAAATACGTATGACAAAGAGACAATCAAGGGGAGGATTGTCTTG CCTATAAAAGGAGATATGGTTATTGACAAATTAAATGGGAATTCCACCCCAGAAAAGACAGATGGGAGTT CCTCTACCACCTCTCCTCCTTCCTATGACAGTGTAACAAAACCAGATAAGGAAAAGTTTGAGAAAGACAA ACCAGAAAAAGAAAGCAAAGGGAAAGAGGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215436 representing NM_018732
Red=Cloning site Green=Tags(s) MAQALLVPPGPESFRLFTRESLAAIEKRAAEEKAKKPKKEQDIDDENKPKPNSDLEAGKNLPFIYGDIPP EMVSEPLEDLDPYYVSKKTFVVLNKGKAIFRFSATSALYILTPLNPVRKIAIKILVHSLFSMLIMCTILT NCVFMTLSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFSVIVMAYVTEFVDLG NVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQW PPSDSAFEINTTSYFNGTMDSNGTFVNVTMSTFNWKDYIADDSHFYVLDGQKDPLLCGNGSDAGQCPEGY ICVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLVNLIL AVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEEAQAVAAASAASRDFSGIGGLGELLESSSEASK LSSKSAKEWRNRRKKRRQREHLEGNHRPEGDRFPKSESEDSVKRRSFLFSLDGNPLSGDKKLCSPHQSLL SIRGSLFSPRRNSKTSIFSFRGRAKDVGSENDFADDEHSTFEDSESRRDSLFVPHRPGERRNSNGTTTET EVRKRRLSSYQISMEMLEDSSGRQRAMSIASILTNTMEELEESRQKCPPCWYRFANVFLIWDCCDSWLKV KHLVNLIVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLTVGNLVFTGIFTAEMVLKIIAMDPYYY FQEGWNIFDGIIVSLSLMELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVL AIIVFIFAVVGMQLFGKSYKECVCKINEDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTM CLIVFMLVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDNEMNNLQIAVGRMQKGIDYVKNKIRECFRKA FFRKPKVIEIHEGNKIDSCMSNNTGVVEISKELNYLKDGNGTTSGVGTGSSVEKYVIDENDYMSFINNPS LTVTVPIAVGESDFENLNTEEFSSESELEESKEKLNATSSSEGSTVDVAPPREGEQAEIEPEEDLKPEAC FTEGCIKKFPFCQVSTEEGKGKIWWNLRKTCYSIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKT MLEYADKVFTYIFILEMLLKWVAYGFQTYFTNAWCWLDFLIVDVSLVSLVANALGYSELGAIKSLRTLRA LRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNMTTGSMFDMSEV NNFSDCQALGKQARWKNVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRDVKLQPVYEENLYMYLYF VIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGM VFDFVTRQVFDISIMILICLNMVTMMVETDDQSKYMTLVLSRINLVFIVLFTGEFLLKLISLRYYYFTIG WNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNI GLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDP DAIHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWE KFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMD ALRIQMEDRFMASNPSKVSYEPITTTLKRKQEEVSAAIIQRNYRCYLLKQRLKNISNTYDKETIKGRIVL PIKGDMVIDKLNGNSTPEKTDGSSSTTSPPSYDSVTKPDKEKFEKDKPEKESKGKEV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018732 |
| ORF Size | 5841 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018732.3, NP_061202.3 |
| RefSeq Size | 9560 bp |
| RefSeq ORF | 5844 bp |
| Locus ID | 20269 |
| UniProt ID | A2ASI5 |
| Gene Summary | Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:29142310). May contribute to the regulation of serotonin/5-hydroxytryptamine release by enterochromaffin cells (PubMed:29142310). In pancreatic endocrine cells, required for both glucagon and glucose-induced insulin secretion (PubMed:25172946).[UniProtKB/Swiss-Prot Function] |
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