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Rbm15 (NM_001045807) Mouse Tagged ORF Clone
CAT＃: MG215320
- TrueORF®
Rbm15 (tGFP-tagged) - Mouse RNA binding motif protein 15 (Rbm15), (10ug)
ORF Plasmid: DDK
"NM_001045807" in other vectors (4)
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CNY 9,310.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C230088J01Rik; mKIAA1438 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215320 representing NM_001045807
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGTCTGCGGGGCGGGAGCCTTTGCCGCGGCGGAGTCCAAGATGGCGGCGTGCGAGTCCGCTGTGTG AAACGAGCGCGGGGTGGCGGGTTAGTCAGCTCCGCAGAGACGACCTCCGGCGACCCTCAACAATGAAAGG AAAGGAGCGCTCGCCGGTCAAGCCCAAGCGCTCCCGTGGCGGTGAGGACTCCAGTTCTCGTGGGGAGCGG AGCAAGAAGTTAGGGGGCTCCGGCGGCAGCAATGGGAGCAGCAGCGGGAAGACGGACAGCGGCGGGTCGC GGCGGAGCCTTCATCTGGACAAGTCCAGCAGCCGGGGCGGCAGCCGCGAGTACGAGACCGGCGGGGGCAG CTCCAGTAGCCGCTTGCACAGTTACAGCTCCCCGAGCACCAAAAATTCCTCAGGCGGGGGCGAGTCGCGC AGCAGCTCCCGGGGTGGAGGCGGGGAGTCACGTTCCTCCGGGGCCGCCTCCTCCGCGCCCGGCGGCGGGG ACGGCGTGGAGTACAAGACCCTGAAGATCAGCGAGCTGGGGTCCCAGCTGAGCGACGAGGCGGTGGAGGA CGGGCTGTTTCACGAGTTCAAACGCTTCGGTGATGTAAGTGTCAAAATCAGTCACCTCTCAGGTTCTGGC AGCGGGGATGAGCGGGTAGCCTTTGTGAACTTCCGGAGGCCAGAGGACGCGAGGGCGGCCAAGCATGCCA GAGGCCGCCTGGTGCTCTATGACCGGCCTCTGAAGATAGAAGCCGTGTACGTGAGCCGGCGCCGCAGCCG CTCCCCGTTAGACAAAGATGCTTACGCCCCGTCATCCAGCGTGGTCGGGACCTCAGTCGGTAGCCACCGG CACGCCCCTGGAGGAGGGGGAGGTCAGAGGTCGCTTTCCCCTGGAGGTGCTGCTCTGGGATACAGAGACT ACCGGTTGCAGCAGTTGGCCCTGGGCCGCCTGCCTCCTCCACCCCCGCCACCATTGCCCCGAGAGCTGGA AAGAGAACGAGACTATCCGTTCTATGACCGGGTGCGCCCGGCCTACAGCCTGGAGCCGAGGGTGGGAGCT GGAGCAGGTGCGGCTCCTTTCCGAGAAGTGGATGAGATATCACCTGAGGACGATCAGCGTGCCAACCGGA CACTTTTCTTGGGCAACTTAGATATCACTGTGACGGAAAATGATCTCAGAAGGGCTTTTGATCGTTTTGG AGTCATCACAGAAGTGGACATTAAGAGGCCTTCTCGAGGCCAGACCAGTACCTATGGCTTTCTCAAATTT GAGAATCTAGACATGTCTCACAGGGCCAAACTTGCAATGTCTGGCAAAATTATAATTCGGAATCCAATCA AGATTGGTTATGGTAAAGCCACACCCACTACCCGACTTTGGGTGGGTGGTCTGGGACCCTGGGTGCCTCT TGCTGCCCTGGCACGAGAATTTGACCGATTTGGCACCATTCGAACCATAGACTACCGTAAAGGTGACAGT TGGGCATATATACAGTATGAAAGTTTGGATGCAGCTCATGCTGCCTGGACCCATATGCGTGGATTCCCAC TTGGTGGCCCAGATCGTCGCCTCAGGGTAGACTTTGCAGACACAGAACATCGTTACCAGCAGCAATATCT TCAGCCTCTGCCCTTGACCCACTATGAACTAGTGACGGATACTTTTGGACATAGAGCACCTGACCCTTTG AGGAGTGCTCGGGACAGGACACCACCCTTACTATACAGAGATCGTGATAGGGACCTTTATACTGATTCTG ACTGGGTGCCACCCCCACCACCGGTTCGAGAACGCAGTGCTCGGGCTGCAACTAGTGCTGTCACTGCTTA TGAGCCACTAGATAGCTTGGATCGAAGGAGGGATGGCTGGTCACTGGACAGGGACAGAGGTGATCGGGAC TTGCCCAGCAGCAGGGACCAGCCAAGGAAGCGAAGGCTGCCTGAGGAAAGTGGAGGACGGCATCTTGATA GATCACCTGAGAGTGAACGGCCTCGAAAACAGCGTCACTGCACTCCTTCTCCTGACCGAAGTCCAGAACT GAGCAGTAACAGAGATCGCTACAACAGTGACAATGATCGATCATCTCGTCTTCTCCTCTTGGAAAGGTCT TCCCCAGTCAGAGATAGACGGGGCAGTTTGGAGAAGAGCCAGAGCGACAAGCGAGACCGTAAAAACTCTG CATCAGCTGAACGGGATAGGAAGCATCGGACAGCTGCTCCCACAGAGGGAAAAAACCCTCTGAAAAAGGA AGACCGATCTGATGGCAATGCACCCAGTGCCAGCACTTCTTCATCAAAGCAGAAGCCACCTTCCCAGAAA CAGGATGGAGGGACAGCTCCTGTGGCAGCATCCTCTCCCAAACTCTGTTTGGCTTGGCAGGGCATGCTTC TACTGAAGAACAGCAACTTCCCTTCCAACATGCATCTGTTGCAGGGTGACCTCCAAGTTGCTAGCAGTCT ACTTGTGGAGGGCTCAACTGGAGGCAAAGTGGCCCAACTCAAGATCACTCAGCGGCTCCGTTTGGACCAA CCCAAGTTGGATGAAGTAACTCGACGCATCAAAGTGGCAGGGCCTAATGGTTATGCCATTCTCCTGGCTG TACCTGGAAGTTCTGACAGCCGATCTTCCTCTTCCTCAGCCACATCAGACACTGCCGCCTCTACTCAGAG GCCACTTAGGAACCTTGTGTCCTATTTAAAGCAAAAGCAGGCAGCTGGGGTGATCAGCCTCCCTGTGGGA GGCAATAAAGACAAGGAAAACACCGGGGTTCTTCATGCCTTCCCACCTTGTGAGTTCTCTCAGCAGTTCC TGGATTCCCCTGCCAAGGCACTGGCCAAATCTGAAGAAGATTACCTGGTCATGATCATTGTCCGTGCAAA ACTGGTGAACAGCGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215320 representing NM_001045807
Red=Cloning site Green=Tags(s) MRSAGREPLPRRSPRWRRASPLCETSAGWRVSQLRRDDLRRPSTMKGKERSPVKPKRSRGGEDSSSRGER SKKLGGSGGSNGSSSGKTDSGGSRRSLHLDKSSSRGGSREYETGGGSSSSRLHSYSSPSTKNSSGGGESR SSSRGGGGESRSSGAASSAPGGGDGVEYKTLKISELGSQLSDEAVEDGLFHEFKRFGDVSVKISHLSGSG SGDERVAFVNFRRPEDARAAKHARGRLVLYDRPLKIEAVYVSRRRSRSPLDKDAYAPSSSVVGTSVGSHR HAPGGGGGQRSLSPGGAALGYRDYRLQQLALGRLPPPPPPPLPRELERERDYPFYDRVRPAYSLEPRVGA GAGAAPFREVDEISPEDDQRANRTLFLGNLDITVTENDLRRAFDRFGVITEVDIKRPSRGQTSTYGFLKF ENLDMSHRAKLAMSGKIIIRNPIKIGYGKATPTTRLWVGGLGPWVPLAALAREFDRFGTIRTIDYRKGDS WAYIQYESLDAAHAAWTHMRGFPLGGPDRRLRVDFADTEHRYQQQYLQPLPLTHYELVTDTFGHRAPDPL RSARDRTPPLLYRDRDRDLYTDSDWVPPPPPVRERSARAATSAVTAYEPLDSLDRRRDGWSLDRDRGDRD LPSSRDQPRKRRLPEESGGRHLDRSPESERPRKQRHCTPSPDRSPELSSNRDRYNSDNDRSSRLLLLERS SPVRDRRGSLEKSQSDKRDRKNSASAERDRKHRTAAPTEGKNPLKKEDRSDGNAPSASTSSSKQKPPSQK QDGGTAPVAASSPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQ PKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSATSDTAASTQRPLRNLVSYLKQKQAAGVISLPVG GNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRAKLVNSG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001045807 |
| ORF Size | 2886 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001045807.1, NP_001039272.1 |
| RefSeq Size | 3270 bp |
| RefSeq ORF | 2889 bp |
| Locus ID | 229700 |
| UniProt ID | Q0VBL3 |
| Gene Summary | RNA-binding protein that acts as a key regulator of N6-methyladenosine (m6A) methylation of RNAs, thereby regulating different processes, such as hematopoietic cell homeostasis, alternative splicing of mRNAs and X chromosome inactivation mediated by Xist RNA (PubMed:29535189). Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29535189). Plays a key role in m6A methylation, possibly by binding target RNAs and recruiting the WMM complex (PubMed:29535189). Involved in random X inactivation mediated by Xist RNA: acts by binding Xist RNA and recruiting the WMM complex, which mediates m6A methylation, leading to target YTHDC1 reader on Xist RNA and promoting transcription repression activity of Xist (By similarity). Required for the development of multiple tissues, such as the maintenance of the homeostasis of long-term hematopoietic stem cells and for megakaryocyte (MK) and B-cell differentiation (PubMed:17283045, PubMed:17376872, PubMed:18981216, PubMed:25468569). Regulates megakaryocyte differentiation by regulating alternative splicing of genes important for megakaryocyte differentiation; probably regulates alternative splicing via m6A regulation (By similarity). Required for placental vascular branching morphogenesis and embryonic development of the heart and spleen (PubMed:18981216). Acts as a regulator of thrombopoietin response in hematopoietic stem cells by regulating alternative splicing of MPL (PubMed:25468569). May also function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing (By similarity). High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing (By similarity). May be implicated in HOX gene regulation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222884 | Rbm15 (untagged) - Mouse RNA binding motif protein 15 (Rbm15), (10ug) |
CNY 12,350.00 |
|
| MR215320 | Rbm15 (Myc-DDK-tagged) - Mouse RNA binding motif protein 15 (Rbm15) |
CNY 7,056.00 |
|
| MR215320L3 | Lenti ORF clone of Rbm15 (Myc-DDK-tagged) - Mouse RNA binding motif protein 15 (Rbm15) |
CNY 10,360.00 |
|
| MR215320L4 | Lenti ORF clone of Rbm15 (mGFP-tagged) - Mouse RNA binding motif protein 15 (Rbm15) |
CNY 10,360.00 |
