United States
Germany
Japan
United Kingdom
China
Otud7b (NM_001025613) Mouse Tagged ORF Clone
CAT＃: MG214175
- TrueORF®
Otud7b (tGFP-tagged) - Mouse OTU domain containing 7B (Otud7b) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001025613" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 10,840.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2900060B22Rik; 4930463P07Rik; AI462125; CEZANNE; Trabid2; Za20d1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025613 |
| ORF Size | 2520 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025613.1, NP_001020784.1 |
| RefSeq Size | 8101 bp |
| RefSeq ORF | 2523 bp |
| Locus ID | 229603 |
| UniProt ID | B2RUR8 |
| Gene Summary | Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B (PubMed:23334419). Negatively regulates mucosal immunity against infections (PubMed:23334419). Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241). Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (PubMed:26903241). Mediates deubiquitination of EGFR (By similarity). Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro); however the physiological significance of these data are unsure. Hydrolyzes both linear and branched forms of polyubiquitin (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222023 | Otud7b (untagged) - Mouse OTU domain containing 7B (Otud7b), transcript variant 1, (10ug) |
CNY 10,740.00 |
|
| MR214175 | Otud7b (Myc-DDK-tagged) - Mouse OTU domain containing 7B (Otud7b), transcript variant 1 |
CNY 9,240.00 |
|
| MR214175L1 | Lenti ORF clone of Otud7b (Myc-DDK-tagged) - Mouse OTU domain containing 7B (Otud7b), transcript variant 1 |
CNY 11,640.00 |
|
| MR214175L2 | Lenti ORF clone of Otud7b (mGFP-tagged) - Mouse OTU domain containing 7B (Otud7b), transcript variant 1 |
CNY 9,030.00 |
|
| MR214175L3 | Lenti ORF clone of Otud7b (Myc-DDK-tagged) - Mouse OTU domain containing 7B (Otud7b), transcript variant 1 |
CNY 9,030.00 |
|
| MR214175L4 | Lenti ORF clone of Otud7b (mGFP-tagged) - Mouse OTU domain containing 7B (Otud7b), transcript variant 1 |
CNY 9,030.00 |
