United States
Germany
Japan
United Kingdom
China
Ddx31 (NM_001033294) Mouse Tagged ORF Clone
CAT＃: MG212865
- TrueORF®
Ddx31 (tGFP-tagged) - Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31 (Ddx31), (10ug)
ORF Plasmid: DDK
"NM_001033294" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5830444G11Rik; Gm997 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212865 representing NM_001033294
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGGCAAGCAGAGGCTACCAAGAGAAAGCACCAAGTGTCCAGTGATGCTCCACCAGCAAAACGGA GGAGTGAAATATCCTCTGTTTTGGCCAAGAAAGCCAGTGATAAAGAAACTCAGAGAACATTTAAAGGGAG TACACACAAAACGTTTCCTCCAAAGAAGTATTTGGACAGTATAGGCAATGGAAGGCAAGAGGAGAAGCCA TGCATCAAGACTTCATCGCTGTTTAAAAACAACCCTGAGATCCCAGAACTCCACAGCACTGTAGTGAAGC AGGCTCGAGAGCAAGTGTTCTCTCCAGAAGCTTTCCAGGAGCTGGACCTCCACCCACATCTAATTTCTAC AATAAATACAGTCTTAAAAATGTCCAGTATGACCAGTGTTCAGAAGCAGAGTATCCCAGTGCTGCTGGAA GGCAGAGATGCCCTAGTGCGGTCCCAGACGGGCTCAGGTAAAACTCTTGCCTATTGCGTCCCTGTGGTCC AGTCTCTTCAAGCACTGACATCAAAAATACAGCGCAGTGATGGACCGTATGCTTTGGTCCTAGTGCCCAC CAGAGAGTTGGCTCTGCAAAGCTTTGACACTGTTCAGAAGCTACTTAAGCCATTCACCTGGATTGTTCCT GGAGTGTTGATGGGTGGAGAGAAGAGGAAATCGGAAAAAGCTAGACTCCGAAAAGGCATAAATATCCTTA TCTCCACTCCTGGCCGTCTAGTGGATCATATAAAATCCACAAAGAACCTTCACTTTAACCGAATACGGTG GCTGATTGTGGATGAAGCAGACAGGATCTTGGATTTGGGTTTTGAGAAGGACATCACAGTGATTCTGAAT GCTGTCAATGCTGAGTGTCAGAAACGGCAGAATGTCCTCCTGTCAGCGACACTCACAGAAGGTGTAACAC GGCTAGTTGATATCAGTTTGCACAATCCAGTCAGTATCTCTGTCCTGGACAAAAACTGCAACCAGCCCAA TCCTAAGGAAGTTGCTAGTATCCAGCTCAACAGCTTTGCCATACCAGAGAGTCTCGACCAGCATGTGGTG TTGGTTCCCAGCAAGCTACGTCTTGTCTGCCTTGCAGCCTTCATCCTGCAAAAGTGCAAGTTTGAGAAGA ACCAGAAGATGATTGTCTTTTTCTCAAGTTGTGAATTGGTGGAGTTCCACTACAGCCTCTTCCTTCACAC CCTGCTTTGCCACTCAGGGACCCCTACTTCAGAGCATCTGCCATCTGCCTCCTGGCCACTGAAATTCCTT CGGCTGCATGGCAACATGGAACAGGAGGAAAGAACATCCGTGTTTCATGAGTTCTCCCATTCTGAAACGG GAGTCTTGCTCTGCACGGATGTTGCATCTCGAGGCTTGGATCTCCCACAAGTCACCTGGATTGTTCAGTA CAGTGCTCCGTCTTCACCTGCAGAGTACATTCACCGCATCGGAAGAACTGCCCGGATTGGCTGTCATGGG AGCAGCCTGCTCATTTTGGCTCCTTCGGAGGCAGAATATGTCAACTCGTTGGCTTCTCACAAAATCAATG TTGGTGAGATTAAGATGGAAGATATTTTGGCTGTCCTGGCAAAGGATGATTGTTTTAAAAGGAGACAAAG GGGAGCCCAGAAATCCCGTGCCAGTGGCCCCCAGGAAATCCGAGAACGAGCCACAGTATTGCAGACAGTG TTTGAAGATTATGTGCACTCCAGTCAGAGGATGGTCTCCTGGGCTAAGAAAGCCCTGCAGTCCTTCATCC GAGCCTATGCCACCTACCCCAAGGAGCTGAAGTCCATCTTCCATGTCCGAGCCCTACACCTTGGGCACGT GGCTAAGAGTTTTGGACTAAGAGATGCTCCTAGAAACCTCAGTGTCTCTGCTGTGAAGAAGGCAAGCTTA AAAAGGCCTCACCCTCGGAGGAAGACGCAGAGGAAGCAGCACCTTGTGCCTGCTGAAGTCCTGCACTCAG AACACTCAAGTGGGCTGGAGGGTGGTGCCACGAAGTGCAGAAAGCAAGGCAAACAACAGGGCCTTCAGGT GGCACCCAGCAAGCCGGGGCCGTGGAGAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212865 representing NM_001033294
Red=Cloning site Green=Tags(s) MKRQAEATKRKHQVSSDAPPAKRRSEISSVLAKKASDKETQRTFKGSTHKTFPPKKYLDSIGNGRQEEKP CIKTSSLFKNNPEIPELHSTVVKQAREQVFSPEAFQELDLHPHLISTINTVLKMSSMTSVQKQSIPVLLE GRDALVRSQTGSGKTLAYCVPVVQSLQALTSKIQRSDGPYALVLVPTRELALQSFDTVQKLLKPFTWIVP GVLMGGEKRKSEKARLRKGINILISTPGRLVDHIKSTKNLHFNRIRWLIVDEADRILDLGFEKDITVILN AVNAECQKRQNVLLSATLTEGVTRLVDISLHNPVSISVLDKNCNQPNPKEVASIQLNSFAIPESLDQHVV LVPSKLRLVCLAAFILQKCKFEKNQKMIVFFSSCELVEFHYSLFLHTLLCHSGTPTSEHLPSASWPLKFL RLHGNMEQEERTSVFHEFSHSETGVLLCTDVASRGLDLPQVTWIVQYSAPSSPAEYIHRIGRTARIGCHG SSLLILAPSEAEYVNSLASHKINVGEIKMEDILAVLAKDDCFKRRQRGAQKSRASGPQEIRERATVLQTV FEDYVHSSQRMVSWAKKALQSFIRAYATYPKELKSIFHVRALHLGHVAKSFGLRDAPRNLSVSAVKKASL KRPHPRRKTQRKQHLVPAEVLHSEHSSGLEGGATKCRKQGKQQGLQVAPSKPGPWRE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033294 |
| ORF Size | 2061 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033294.3, NP_001028466.2 |
| RefSeq Size | 3328 bp |
| RefSeq ORF | 2064 bp |
| Locus ID | 227674 |
| UniProt ID | Q6NZQ2 |
| Gene Summary | Probable ATP-dependent RNA helicase (By similarity). Plays a role in ribosome biogenesis and TP53/p53 regulation through its interaction with NPM1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220531 | Ddx31 (untagged) - Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31 (Ddx31), (10ug) |
CNY 8,840.00 |
|
| MR212865 | Ddx31 (Myc-DDK-tagged) - Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31 (Ddx31) |
CNY 5,032.00 |
|
| MR212865L3 | Lenti ORF clone of Ddx31 (Myc-DDK-tagged) - Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31 (Ddx31) |
CNY 7,700.00 |
|
| MR212865L4 | Lenti ORF clone of Ddx31 (mGFP-tagged) - Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 31 (Ddx31) |
CNY 7,700.00 |
