Myh11 (BC026142) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	AV071570; SM1; SM2; smMHC
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212105 representing BC026142 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGAAAGGGCAGCTCAGCGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATGAACAGCC CAATGGCTCAGGCCGACTGGGTAGCCAAGAAGCTGGTGTGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTCGTGGAGTTGGTGGAAAATGGAAAGAAGGTCACA GTTGGCAAAGATGACATCCAAAAAATGAACCCACCCAAGTTCTCTAAGGTGGAGGACATGGCAGAGCTGA CGTGCCTCAATGAGGCCTCTGTGCTGCACAACCTGAGGGAGCGATACTTCTCAGGCCTCATCTATACCTA CTCTGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGTACCTACCCATCTACTCAGAAAAGATCGTGGAC ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCCGACACAGCCTACAGAA GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCCGGAAAGACAGAGAA CACAAAGAAAGTCATACAGTACTTGGCTGTGGTAGCATCCTCCCACAAGGGCAAGAAAGACAGCAGCATC ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCGAAAACCG TCAAGAACGACAACTCCTCTCGCTTTGGCAAGTTCATTCGCATCAACTTCGATGTCACTGGTTACATTGT AGGTGCCAATATTGAAACATATCTTCTGGAAAAGTCTAGGGCTATTCGACAGGCTAGGGATGAGAGAACA TTTCACATCTTCTACTATCTGCTCGCCGGAGCCAAGGAAAAGATGAAAAGTGACCTGCTTTTGGAGAGCT TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA GGAGACACTAGAAGCCATGTCTATCATGGGCTTCAATGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA TCATCCGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG ATAACACAGCGGCTCAGAAAGTTTGCCACCTTGTGGGGATTAATGTGACAGATTTCACTAGAGCCATCCT GACCCCACGTATCAAAGTTGGACGGGATGTGGTGCAGAAGGCTCAGACCAAAGAACAGGCTGACTTTGCC ATCGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTAAACAAGGCCT TGGACAAGACCCATCGGCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCCGGGTTTGAAATCTTTGA GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACG ATGTTCATCCTGGAGCAGGAAGAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTCGGCCTGG ACCTGCAGCCTTGTATTGAGCTGATTGAGCGGCCGAACAACCCTCCCGGTGTGCTGGCCCTGCTGGATGA AGAATGCTGGTTCCCCAAAGCTACAGACAAGTCTTTTGTGGAGAAGCTATGCTCAGAGCAGGGAAATCAC CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG TGGACTACAATGCAAGTGCCTGGCTGACCAAGAACATGGACCCGCTAAATGACAATGTGACATCACTCCT CAATGCCTCCTCTGACAAGTTTGTGGCTGACCTGTGGAAGGATGTGGACCGCATTGTGGGGCTGGACCAG ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG GCCAGCTCTACAAAGAGCAGTTGGGGAAACTGATGACTACACTGCGCAATACCACACCTAACTTTGTGCG CTGCATCATTCCCAACCATGAGAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTGCTGGAACAGCTGCGC TGCAACGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT TCCGGCAACGCTATGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGATGGAAAGCAAGCCTG CATTCTCATGATCAAAGCCCTCGAACTTGACCCCAACTTGTACAGGATTGGGCAGAGCAAAATCTTCTTC CGAACGGGGGTCCTGGCCCACCTAGAGGAGGAACGAGACCTGAAGATTACTGATGTCATCATGGCATTCC AGGCAATGTGTCGTGGCTACCTTGCCAGAAAGGCCTTCACCAAGAGGCAGCAACAGCTGACAGCCATGAA GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGCGGCTCTTCACCAAA GTAAAGCCATTGCTCCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAAAAGA TCAAGGAGCGGCAGCAGAAGGCAGAGACTGAGTTGAAGGAGCTGGAGCAGAAGCACACTCAGCTGGCTGA GGAGAAGACTCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAGGAGATGCGG GTCCGGTTGGCAGCCAAGAAGCAGGAACTGGAGGAGATCCTACATGAGATGGAGGCCCGCCTGGAGGAAG AGGAAGACCGGGGCCAGCAACTACAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTAGACCTGGAGGA GCAACTGGAGGAGGAAGAGGCCGCCAGACAGAAACTACAGCTAGAGAAGGTCACGGCTGAGGCCAAGATC AAGAAACTGGAGGATGACATCTTGGTTATGGATGATCAGAACAGTAAACTTTCAAAAGAACGAAAACTCC TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA GCTGAAGAGCAAGCATGAGTCTATGATCTCAGAGCTGGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCATGAGCAGATCGCTGACT TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC CAGGCTTGATGAAGAGATCACCCAGAAAAACAATGCCCTAAAGAAGATTCGCGAGCTAGAGGGCCATATC TCAGACCTACAAGAGGACCTAGACTCAGAGCGGGCTGCCAGGAACAAGGCCGAGAAGCAGAAGCGAGACC TGGGGGAGGAGCTGGAGGCACTCAAGACGGAGCTGGAGGATACGCTGGACAGCACAGCTACCCAGCAGGA GCTCAGAGCCAAGAGGGAACAGGAGGTGACAGTGCTGAAGAAGGCCCTGGATGAGGAGACGCGGTCCCAT GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAGC AGTTCAAAAGGGCCAAGGCAAACCTGGACAAAAGCAAGCAGACACTGGAGAAGGAGAACGCGGACCTGGC TGGGGAGCTGCGTGTCCTGGGCCAGGCGAAGCAGGAGGTGGAACACAAGAAGAAGAAGCTGGAGGTGCAG CTGCAGGATCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGGCTGAGCTCAGTGACAAGGTCCACA AGCTACAGAATGAAGTGGAGAGTGTCACTGGCATGCTCAATGAGGCAGAGGGCAAAGCCATCAAACTGGC CAAAGATGTGGCTTCCCTTGGATCCCAGCTTCAGGACACCCAAGAGCTGCTCCAAGAAGAAACCCGGCAG AAGCTCAATGTGTCTACCAAGCTGCGTCAGTTGGAAGATGAAAGGAACAGCCTGCAGGACCAGCTGGATG AGGAGATGGAGGCTAAGCAAAACCTGGAGCGTCATGTCTCAACACTGAACATTCAGCTCTCAGACTCAAA GAAGAAGCTGCAGGACTTTGCAAGTACCATCGAGGTCATGGAGGAGGGGAAGAAGAGGTTACAGAAAGAG ATGGAGGGCCTCAGCCAGCAGTATGAGGAGAAGGCGGCTGCCTATGACAAACTGGAGAAAACCAAGAACA GGCTCCAGCAGGAGCTGGACGACCTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA AAAGAAGCAGAAGAAATTTGACCAGTTGTTAGCTGAGGAGAAGAACATCTCCTCCAAGTATGCGGATGAG AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAGACAAAGGCTTTGTCTCTAGCCCGGGCCCTGGAGG AAGCCCTGGAAGCCAAAGAAGAGCTGGAGAGGACCAACAAGATGCTCAAAGCTGAGATGGAAGACCTGGT CAGCTCCAAGGATGATGTAGGCAAGAACGTGCATGAACTGGAGAAGTCCAAGCGTGCCTTGGAGACCCAG ATGGAAGAGATGAAAACCCAGCTGGAGGAGTTGGAGGATGAGCTGCAGGCCACTGAGGATGCCAAGCTGC GGCTAGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGCGATCTCCAGGCTCGGGATGAACAGAA TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCACGAGTATGAGACAGAACTGGAAGATGAACGGAAG CAGAGAGCTCTGGCGGCGGCAGCTAAGAAGAAGCTGGAAGGGGACCTAAAAGACCTAGAGCTCCAGGCTG ACTCAGCCATCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTACAGGCTCAGATGAAGGACTT CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGGGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG AAAGCCAAGAGTCTGGAGGCAGACCTCATGCAGCTCCAAGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCCGAGGAGCTGGCTAGCAGCTTGTCAGGAAGGAATACACT GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAACTAGAGGAGGAGCTGGAGGAAGAGCAGGGC AACATGGAGGCCATGAGTGATAGAGTGCGCAAGGCCACACTGCAGGCTGAGCAACTGAGCAATGAGCTGG CCACAGAACGCAGCACGGCTCAGAAGAATGAGAGCGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT GCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTCAAGTCCACTGTTGCGGCGCTGGAG GCCAAGATTGCACAGCTGGAGGAGCAGGTTGAACAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGC TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTGCTGCAGGTGGAGGATGAGCGCAAGATGGCAGAGCA GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTGAAGAGGCAGCTGGAAGAGGCAGAG GAGGAGTCCCAGCGCATCAACGCCAACCGCAGGAAGCTGCAGCGGGAGCTAGATGAGGCCACTGAGAGCA ATGAGGCCATGGGCCGTGAGGTGAACGCCCTCAAGAGCAAACTCAGGAGAGGAAACGAGGCTTCATTTGT TCCTTCCAGAAGGGCTGGGGGCCGTAGAGTTATTGAAAACACAGATGGTTCTGAAGAAGAAATGGACGCT CGGGACTCAGACTTCAATGGAACCAAAGCCAGTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212105 representing BC026142 Red=Cloning site Green=Tags(s) MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVT VGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVD MYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSI TGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERT FHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVV SSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFA IEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHT MFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNH PKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQ MAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLR CNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFF RTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTK VKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMR VRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKI KKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSR QELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEITQKNNALKKIRELEGHI SDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSH EAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQ LQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQ KLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKE MEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADE RDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQ MEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERK QRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEK KAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQG NMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALE AKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAE EESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDA RDSDFNGTKASE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026142
ORF Size	5918 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC026142, AAH26142
RefSeq Size	6584 bp
RefSeq ORF	5918 bp
Locus ID	17880

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