Myh11 (BC026142) Mouse Tagged ORF Clone

CAT#: MG212105

  • TrueORF®

Myh11 (tGFP-tagged) - Mouse myosin, heavy polypeptide 11, smooth muscle (cDNA clone MGC:13920 IMAGE:3989495)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 15,580.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AV071570; SM1; SM2; smMHC
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212105 representing BC026142
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGAAAGGGCAGCTCAGCGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATGAACAGCC
CAATGGCTCAGGCCGACTGGGTAGCCAAGAAGCTGGTGTGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC
AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTCGTGGAGTTGGTGGAAAATGGAAAGAAGGTCACA
GTTGGCAAAGATGACATCCAAAAAATGAACCCACCCAAGTTCTCTAAGGTGGAGGACATGGCAGAGCTGA
CGTGCCTCAATGAGGCCTCTGTGCTGCACAACCTGAGGGAGCGATACTTCTCAGGCCTCATCTATACCTA
CTCTGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGTACCTACCCATCTACTCAGAAAAGATCGTGGAC
ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCCGACACAGCCTACAGAA
GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCCGGAAAGACAGAGAA
CACAAAGAAAGTCATACAGTACTTGGCTGTGGTAGCATCCTCCCACAAGGGCAAGAAAGACAGCAGCATC
ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCGAAAACCG
TCAAGAACGACAACTCCTCTCGCTTTGGCAAGTTCATTCGCATCAACTTCGATGTCACTGGTTACATTGT
AGGTGCCAATATTGAAACATATCTTCTGGAAAAGTCTAGGGCTATTCGACAGGCTAGGGATGAGAGAACA
TTTCACATCTTCTACTATCTGCTCGCCGGAGCCAAGGAAAAGATGAAAAGTGACCTGCTTTTGGAGAGCT
TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA
GGAGACACTAGAAGCCATGTCTATCATGGGCTTCAATGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA
TCATCCGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG
ATAACACAGCGGCTCAGAAAGTTTGCCACCTTGTGGGGATTAATGTGACAGATTTCACTAGAGCCATCCT
GACCCCACGTATCAAAGTTGGACGGGATGTGGTGCAGAAGGCTCAGACCAAAGAACAGGCTGACTTTGCC
ATCGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTAAACAAGGCCT
TGGACAAGACCCATCGGCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCCGGGTTTGAAATCTTTGA
GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACG
ATGTTCATCCTGGAGCAGGAAGAGTACCAGCGAGAGGGCATCGAGTGGAACTTCATCGACTTCGGCCTGG
ACCTGCAGCCTTGTATTGAGCTGATTGAGCGGCCGAACAACCCTCCCGGTGTGCTGGCCCTGCTGGATGA
AGAATGCTGGTTCCCCAAAGCTACAGACAAGTCTTTTGTGGAGAAGCTATGCTCAGAGCAGGGAAATCAC
CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG
TGGACTACAATGCAAGTGCCTGGCTGACCAAGAACATGGACCCGCTAAATGACAATGTGACATCACTCCT
CAATGCCTCCTCTGACAAGTTTGTGGCTGACCTGTGGAAGGATGTGGACCGCATTGTGGGGCTGGACCAG
ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG
GCCAGCTCTACAAAGAGCAGTTGGGGAAACTGATGACTACACTGCGCAATACCACACCTAACTTTGTGCG
CTGCATCATTCCCAACCATGAGAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTGCTGGAACAGCTGCGC
TGCAACGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT
TCCGGCAACGCTATGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGATGGAAAGCAAGCCTG
CATTCTCATGATCAAAGCCCTCGAACTTGACCCCAACTTGTACAGGATTGGGCAGAGCAAAATCTTCTTC
CGAACGGGGGTCCTGGCCCACCTAGAGGAGGAACGAGACCTGAAGATTACTGATGTCATCATGGCATTCC
AGGCAATGTGTCGTGGCTACCTTGCCAGAAAGGCCTTCACCAAGAGGCAGCAACAGCTGACAGCCATGAA
GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGCGGCTCTTCACCAAA
GTAAAGCCATTGCTCCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAAAAGA
TCAAGGAGCGGCAGCAGAAGGCAGAGACTGAGTTGAAGGAGCTGGAGCAGAAGCACACTCAGCTGGCTGA
GGAGAAGACTCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAGGAGATGCGG
GTCCGGTTGGCAGCCAAGAAGCAGGAACTGGAGGAGATCCTACATGAGATGGAGGCCCGCCTGGAGGAAG
AGGAAGACCGGGGCCAGCAACTACAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTAGACCTGGAGGA
GCAACTGGAGGAGGAAGAGGCCGCCAGACAGAAACTACAGCTAGAGAAGGTCACGGCTGAGGCCAAGATC
AAGAAACTGGAGGATGACATCTTGGTTATGGATGATCAGAACAGTAAACTTTCAAAAGAACGAAAACTCC
TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA
GCTGAAGAGCAAGCATGAGTCTATGATCTCAGAGCTGGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG
CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCATGAGCAGATCGCTGACT
TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC
CAGGCTTGATGAAGAGATCACCCAGAAAAACAATGCCCTAAAGAAGATTCGCGAGCTAGAGGGCCATATC
TCAGACCTACAAGAGGACCTAGACTCAGAGCGGGCTGCCAGGAACAAGGCCGAGAAGCAGAAGCGAGACC
TGGGGGAGGAGCTGGAGGCACTCAAGACGGAGCTGGAGGATACGCTGGACAGCACAGCTACCCAGCAGGA
GCTCAGAGCCAAGAGGGAACAGGAGGTGACAGTGCTGAAGAAGGCCCTGGATGAGGAGACGCGGTCCCAT
GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAGC
AGTTCAAAAGGGCCAAGGCAAACCTGGACAAAAGCAAGCAGACACTGGAGAAGGAGAACGCGGACCTGGC
TGGGGAGCTGCGTGTCCTGGGCCAGGCGAAGCAGGAGGTGGAACACAAGAAGAAGAAGCTGGAGGTGCAG
CTGCAGGATCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGGCTGAGCTCAGTGACAAGGTCCACA
AGCTACAGAATGAAGTGGAGAGTGTCACTGGCATGCTCAATGAGGCAGAGGGCAAAGCCATCAAACTGGC
CAAAGATGTGGCTTCCCTTGGATCCCAGCTTCAGGACACCCAAGAGCTGCTCCAAGAAGAAACCCGGCAG
AAGCTCAATGTGTCTACCAAGCTGCGTCAGTTGGAAGATGAAAGGAACAGCCTGCAGGACCAGCTGGATG
AGGAGATGGAGGCTAAGCAAAACCTGGAGCGTCATGTCTCAACACTGAACATTCAGCTCTCAGACTCAAA
GAAGAAGCTGCAGGACTTTGCAAGTACCATCGAGGTCATGGAGGAGGGGAAGAAGAGGTTACAGAAAGAG
ATGGAGGGCCTCAGCCAGCAGTATGAGGAGAAGGCGGCTGCCTATGACAAACTGGAGAAAACCAAGAACA
GGCTCCAGCAGGAGCTGGACGACCTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA
AAAGAAGCAGAAGAAATTTGACCAGTTGTTAGCTGAGGAGAAGAACATCTCCTCCAAGTATGCGGATGAG
AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAGACAAAGGCTTTGTCTCTAGCCCGGGCCCTGGAGG
AAGCCCTGGAAGCCAAAGAAGAGCTGGAGAGGACCAACAAGATGCTCAAAGCTGAGATGGAAGACCTGGT
CAGCTCCAAGGATGATGTAGGCAAGAACGTGCATGAACTGGAGAAGTCCAAGCGTGCCTTGGAGACCCAG
ATGGAAGAGATGAAAACCCAGCTGGAGGAGTTGGAGGATGAGCTGCAGGCCACTGAGGATGCCAAGCTGC
GGCTAGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGCGATCTCCAGGCTCGGGATGAACAGAA
TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCACGAGTATGAGACAGAACTGGAAGATGAACGGAAG
CAGAGAGCTCTGGCGGCGGCAGCTAAGAAGAAGCTGGAAGGGGACCTAAAAGACCTAGAGCTCCAGGCTG
ACTCAGCCATCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTACAGGCTCAGATGAAGGACTT
CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGGGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG
AAAGCCAAGAGTCTGGAGGCAGACCTCATGCAGCTCCAAGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA
AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCCGAGGAGCTGGCTAGCAGCTTGTCAGGAAGGAATACACT
GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAACTAGAGGAGGAGCTGGAGGAAGAGCAGGGC
AACATGGAGGCCATGAGTGATAGAGTGCGCAAGGCCACACTGCAGGCTGAGCAACTGAGCAATGAGCTGG
CCACAGAACGCAGCACGGCTCAGAAGAATGAGAGCGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT
GCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTCAAGTCCACTGTTGCGGCGCTGGAG
GCCAAGATTGCACAGCTGGAGGAGCAGGTTGAACAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGC
TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTGCTGCAGGTGGAGGATGAGCGCAAGATGGCAGAGCA
GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTGAAGAGGCAGCTGGAAGAGGCAGAG
GAGGAGTCCCAGCGCATCAACGCCAACCGCAGGAAGCTGCAGCGGGAGCTAGATGAGGCCACTGAGAGCA
ATGAGGCCATGGGCCGTGAGGTGAACGCCCTCAAGAGCAAACTCAGGAGAGGAAACGAGGCTTCATTTGT
TCCTTCCAGAAGGGCTGGGGGCCGTAGAGTTATTGAAAACACAGATGGTTCTGAAGAAGAAATGGACGCT
CGGGACTCAGACTTCAATGGAACCAAAGCCAGTGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212105 representing BC026142
Red=Cloning site Green=Tags(s)

MAQKGQLSDDEKFLFVDKNFMNSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVT
VGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKYLPIYSEKIVD
MYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSI
TGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERT
FHIFYYLLAGAKEKMKSDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFNEEEQLAILKVV
SSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFA
IEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHT
MFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNH
PKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQ
MAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLR
CNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFF
RTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTK
VKPLLQVTRQEEEMQAKEEEMQKIKERQQKAETELKELEQKHTQLAEEKTLLQEQLQAETELYAEAEEMR
VRLAAKKQELEEILHEMEARLEEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKI
KKLEDDILVMDDQNSKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSR
QELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEITQKNNALKKIRELEGHI
SDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTVLKKALDEETRSH
EAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEVQ
LQDLQSKCSDGERARAELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKDVASLGSQLQDTQELLQEETRQ
KLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFASTIEVMEEGKKRLQKE
MEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADE
RDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQ
MEEMKTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERK
QRALAAAAKKKLEGDLKDLELQADSAIKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEK
KAKSLEADLMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQG
NMEAMSDRVRKATLQAEQLSNELATERSTAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALE
AKIAQLEEQVEQEAREKQAATKSLKQKDKKLKEVLLQVEDERKMAEQYKEQAEKGNTKVKQLKRQLEEAE
EESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDA
RDSDFNGTKASE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC026142
ORF Size 5918 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC026142, AAH26142
RefSeq Size 6584 bp
RefSeq ORF 5918 bp
Locus ID 17880
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...