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Nes (NM_016701) Mouse Tagged ORF Clone
CAT＃: MG212093
- TrueORF®
Nes (tGFP-tagged) - Mouse nestin (Nes). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_016701" in other vectors (2)
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CNY 22,520.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA166324; C78523; ESTM46; Ifaprc2; Marc2; RC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212093 representing NM_016701, codon optimized.
Due to the complexity of NM_016701, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCTGCGTAGGGGAGGAGAGCTTTCAAATGTGGGAGTTGAACAGAAGACTCGAGGCCTACCTGA CCCGGGTGAAGACGCTTGAAGAGCAGAATCAGCTTCTGTCCGCAGAGCTCGGAGGACTGCGGGCCCAGAG CGGGGACGCCTCTTGGCGAGCAAGGGCAGATGACGAACTTGCCGCTCTGAGGGTGTTGGTCGATCAGCGC TGGAGGGAGAAGCACGAGGCAGAAGTCCAGCGGGATAACCTGGCCGAAGAACTGGAGTCAGTAGCCGGAA GGTGCCAACAAGTCCGGCTGGCACGGGAGAGAACGATTGAGGAGGCAGCCTGTTCAAGACGGGCTCTGGA GGCGGAGAAAAACGCACGAGGCTGGCTCTCCACTCAAGCAGCCGAGCTCGAGAGAGAGTTGGAGGCACTG CGAGCCTCTCATGAGGAAGAAAGGGCTCATCTTAACGCCCAGGCTGCCTGTACACCAAGAAGACCACCTG CTCCCGCTCACGCCTCACCCATCCGCGCCCCTGAGGTGGAGGAACTTGCTCGACGGCTTGGTGAGGTCTG GCGCGGAGCAGTGCGCGATTACCAGGAGCGAGTCGCTCACATGGAGAGCAGTCTGGGCCAGGCCAGGGAG AGGCTCGGCCAGGCCGTGAGGGGCGCCCGGGAGAGTCGGCTCGAAGTACAACAGCTGCAGGCTGACCGGG ACTCTCTGCAGGAAAGGCGGGAGGCTCTGGAGCAGCGCTTGGAAGGGCGGTGGCAGGACAGACTGCAAGC GACCGAGAAATTCCAACTGGCCGTGGAAGCGCTGGAACAGGAAAAACAGGGACTGCAGTCTCAGATTGCT CAGATCCTGGAGGGGGGGCAACAGTTGGCCCACCTTAAAATGTCTCTTAGCTTGGAGGTAGCAACGTACA GAACACTGCTCGAGGCCGAAAACAGTCGCCTCCAGACTCCAGGCCGATCCAGCCAAGCCTCCCTCGGCTT CCCAGACCCCAAATTGAAGCTGCACTTCCTGGGTATTCCAGAGGACCAGCACCTGGGTTCTGTGTTGCCT GTGTTGTCTCCCACCTCATTTTCCTCACCGCTCCCTAACACCTTGGAAACACCAGTGACAGCTTTCCTGA AGACTCAAGAGTTTCTTAAGGCCAGAACCCCCACACTCGCTTCTACACCTATCCCTCCCATGTCTGAAGC CCCTTACCCCAAGAACGCTGAAGTCAGAGCACAGGACGTACCCCACTCACTTCTGCAGGGCGGGAGACAG CAAGCTCCAGAGCCCCTCTGGGCAGAGGCAACGGTCCCCTCCAGTACGGGAGTCCTCCCAGAGCTCGAGG AACCCGGCGGGGAACAGCCAGACCACTTCCCCGACGACCCCACGTCCCTCGCTCCTCCTCTTAATCCGCA TCACTCCATCCTTGAGGCAAAAGATCGGGAAAGTTCCGAGAGTAGGGTGTCCTCTATCTTTCAAGAGGAG GAGGGGCAGATTTGGGAGTTGGTGAAGAAGGAAGCAGCAACCGAGGTCAAGGTCGAGAATTCCCTGGCGC AGGAGATCCAGGAATCTGGCTTGGATACCGAGGAGATTCAAGATTCTCAGGGCCCACTGCAGATGGAAAC GCTGGAAGCTCTCGGCGACGAACCACTCATGAGTCTTAAAACGCAGAACCACGAAACCCCAGGTAAAGAA AATTGCAATTCTAGTATCGAAGAGAACAGCGGTACAGTGAAAAGCCCTGAGAAAGAAAAGCAGACACCAC TTAAGAGTCTGGAAGAGAAGAACGTGGAGGCTGAAAAAACGTTGGAAAACGGCGTACTGGAACTGTCCAA GCCCCTCGGCGAGGAGGAGCCCAGGATGGAGGACCAGGAGCTCATGAGTCCCGAGCACACCTTGGAAACC GTATCCTTCCTGGGGAAGGAGAACCAAGAGGTTGTAAGGTCCTCAGAGGAGCAGAATCTGGAGTCCCTCA TCACTTTTAAGGAGGAAAGCCAGTACCCACTGGGAGGACCAGAAGCAGAGGATCAGATGCTGGAGAGACT GGTGGAGAAGGAAGACCAGCGGTTCCCTCGAAGTCCGGAGGAGGATCAACAGGCTTTTCGACCTCTCGAG AAGGAAAATCAAGAGCCCCTGCGCTTCGAGGAGGCCGAGGACCAAGTATTGGAGCGCCTTATTGAGAAGG AGAGGCAGGAGTCCCTGAAGAGTCCCGAAGAGGAGGACCAGCAGGCATTCCGGTTGCTTGAGAAGGAAAA CCAAGAACCCCTGCGATTCGAGGATGCTGAGGATCAGGTCTTGGAAAGACTGATCGAGAAAGAAAGGCAG GAGTCTCTGAAGTCACCTGAAGAAGAGGATCAACAGGCTTTCCGACTGCTGGAGAAGGAAAACCAGGAGC CCCTGCGCTTCGAGGAGGCTGAGGACCAGGTACTGGAGCGCCTCGTAGAGAAGGAGTCTCAGGAGTCCCT GAAGAGCCCCGAAGAAGAGGACCAAAGGACCGGCAAGCCTCTGGAGAAGGAAAACCAAGAAAGTCTCAGA TCTCTGGATGAAAACCAGGAAACAATTGTGCTTCTGGAGTCCAAAAACCAGCGGCCCCTGCGCAGCCTCG AGGTGGAGGAAGAGGAACAGCGCATTGTGAAGCCTTTGGAAAAGGTGTCTCAGGTGTCCTTGGAGTCCTT GGAAAAGGAAAACGTGCAATCACCACGGTACCTCGAGGAGGACGATCATATGATTAAAAGTCTGCTGGAG GACAAAACGCATGAGATCCTCGGGTCCTTGGAGGATCGCAACGGAGAGAACTTTATACCACCCGAGAATG AAACCCAGGGATCATTGCGGCCTCCTGAGGAGGAGGACCAGAGAATCGTGAATCACCTGGAGAAGGAGTC CCAGGAGTTCCTCAGATCACCTGAGGCTGAGGAGGAGGAGGAACAGGTGATGGTCCGCAGCCTGGAGGGC GAGAACCACGATCCACTCTCATCCGTGGTTAAAGAGGAACAGATGGCCGAGTCAAAGTTGGAGAATGAGT CTCAGGACTCTAGAAAGTCCCTGGAGGACGAATCACAGGAGACATTCGGTAGCCTCGAAAAGGAGAATCT CGAGTCTCTGAGGAGCCTGGCAGGGCAAGATCAGGAGGAGCAAAAACTCGAGCAGGAAACACAACAGCCT CTGCGGGCTGTCGAAGACGAACAGATGACCGTTAATCCGCCTGAAAAGGTGGATCCCGAGCTGCCGAAGC CCCTGAGAAATGATCAGGAGGTGGTGAGATCTCTGGATAAGGAGAATCAGGAGTCTCTGGTGAGCCTCAA TGAGGGCGGGATGGAAACTGTGAAATCCTCCGAGACTGAGAACATCGAGTCACTGGAGACAGTAGGAGAA TGCCTGGGCAGAAGAAAATCTGTTGATACGCAGGAACCACTGTGGAGCACGGAGGTCACTTCTGAAACTA TTGAACCACTGGAAGATGAAACCCAGGAGCCTTTGGGCTGCGTCGACGAGAACCAGGAAGTGCTTACCCC ACTGGAGCGCGAGAGCCAGGAGCTGAGATCTCTGGGCAAGTGGAACCCTGAAACCGTGGAGTCACCCGGA GGCGTCGAAGACAGTCAGCAGTGCCTCGAAGTGGAGGAGGGTCCCGAGCGCGAGCAACACCAGGAAAGCC TGCGAAGCCTGGGGGAGGTTGAATGGGAGCTGCCTGGATCAGGGAGTCAGCAACGGTGGGAGGATGTAGT TGAAGATGGTGAAGGTCAGGAGGCTAGCCTCGGTGCGACAGGAGTAGAAACCGAAGACAAGGCTGAGCTC CACCTTAGGGGCCAGGGTGGCGAAGAGAAGGCTGTTGAGGAGGGTGAACTGCTGCAGGATGCAGTTGGGG AGGCATGGAGCCTTGGCTCAAGCGAGCCAAAGGAGCAAAGGGTGCCCGCAGAGCCTCTCGACGACCTCGA GGGGCAACCCGAACAAACTGGAACTCTTGAAGTGCCAGTTGCCCAAGGGATGCCAGAAGCCACAGAACAG GACGAAGACAGGGCCCAGGCTGGCGAGCAGGATAGTGTTGAGGTTACACTCGGGTTGGAGGCCGCTAGGG CTGGCTTGGAACTGGAACAGGAAGTAGTGGGCCTCGAGGACCCACGCCACTTCGCCAGGGAGGAGGCCAT CCACCCATCTCTCGGCGAAGAAAGTGTGAAAGCCAAGATTGACCAGGGCCTCGAGGAGCCTGGCAAAGAG CCTAAGGAGGCTGGAGCACTGGACTCCGGCATTCCCGAGCTGCCTAAGACAAGCTCTGAGACTCTCGAAT GCAAAGGGTGGGAAGAGTCTGGGGAGGGTTGGGGAGAGGAAGAAGCCTCTCTTGAAACCAGTGATCACGA GGGCAGTCACGCCCCCCAGCCCAGACCTCCGAAGACTGAGGAAGATGAGGGTCTGCAGGCCGCACTGACA GTTCCCGGACCCAAGTTGTTGGAGCCCTGCTCACCTATCCCCATTCTTACCGATGCCCACGAACTTCAGC CACAGGCTGAAGGGATCCAGGAAGCGGGGTGGCAACCTGAGGCTGGAACAGAGGCACTCGGGCGCGTTGA GGATGAGCCAGAATTTGGAAGAGGCGAGATCCCGGAAGGACTGCAAGACTGGGAGGAGGGTAGAGAGGAC AGTGAAGCTGACGAACTTGGCGAAACCCTGCCAGATTCTACCCCATTGGGCCTGTATCTCAAGAGTCCTG CATCTCCTAAGTGGGAGCAGGCCGGAGAGCAGCGCCTGTTCCCCCAGGGCGAGGCGCGAAAGGAAGGCTG GAGTCCAGCAGCACTGGCGGCTCAAGGATTGTCAGACCCTCCCGAGGAGGAGCAGCAGGGACACGACTCT GATCTGTCCAGCGAGGAGTTTGAGGATCTCGGGACTGAGGCATCTCTGCTCCCTGGAGTCCCCAAGGAGG TGAGCGACCACCTGGGTCAGGAACCTCCAGTTCTGCAACCCGCTTGCTGGGACCAGGGCGGCGAGAGCGA TGGCTTCGCCGATGAAGAGGAGTCCGGCGAGGAGGGAGAGGAGGAAGATGCAGATGAAGAGGAGGGCGCA GAATCCGGAACACAGTGGTGGGGACCGGGCCCCTCAGGCGGTGGAGTCAAAGTTCAGGATGTCACACAGC GAGGGGATTTGGAGCACGAATCAGTTGGAGACTCTGGGCTGTGGGATGACGGGCTCAGTGGGGCCGCCGC TAATGTCCTGGTAACCGCTCTCGAGACAGTGTCCCAGGACTCAGCGGAGCCCAGCGGCTCCGAAGGCTCT GAGTCAGCCTCTCTGGAAGGTGAAGAGGGGCAAGCAATTGACCACCTCGATGCTCCCCAGGAGGTAACAA GCGTCGTACCTGGCGCAGGAGATACCTTTGATATTTCTGGACAGGGCCCGAACCTCGAGTCTGAGCAAGT GAACGGGCGAATGGAAAATGGGTTGGAGCAGGCCGAAGGCCAGGTTGTGCTTCATGGCGACGAGGACCAG GGAATTCCACTCCAGGAACAGGGTACACTGAAGGCGCCACTGGTGGGTTCCCCCGTTCACCTTGGCCCTT CCCAGCCACTCAAGTTCACACTGTCCGGGGTAGATGGAGATTCTTGGAGTTCTGGCGAAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212093 representing NM_016701
Red=Cloning site Green=Tags(s) MEGCVGEESFQMWELNRRLEAYLTRVKTLEEQNQLLSAELGGLRAQSGDASWRARADDELAALRVLVDQR WREKHEAEVQRDNLAEELESVAGRCQQVRLARERTIEEAACSRRALEAEKNARGWLSTQAAELERELEAL RASHEEERAHLNAQAACTPRRPPAPAHASPIRAPEVEELARRLGEVWRGAVRDYQERVAHMESSLGQARE RLGQAVRGARESRLEVQQLQADRDSLQERREALEQRLEGRWQDRLQATEKFQLAVEALEQEKQGLQSQIA QILEGGQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGRSSQASLGFPDPKLKLHFLGIPEDQHLGSVLP VLSPTSFSSPLPNTLETPVTAFLKTQEFLKARTPTLASTPIPPMSEAPYPKNAEVRAQDVPHSLLQGGRQ QAPEPLWAEATVPSSTGVLPELEEPGGEQPDHFPDDPTSLAPPLNPHHSILEAKDRESSESRVSSIFQEE EGQIWELVKKEAATEVKVENSLAQEIQESGLDTEEIQDSQGPLQMETLEALGDEPLMSLKTQNHETPGKE NCNSSIEENSGTVKSPEKEKQTPLKSLEEKNVEAEKTLENGVLELSKPLGEEEPRMEDQELMSPEHTLET VSFLGKENQEVVRSSEEQNLESLITFKEESQYPLGGPEAEDQMLERLVEKEDQRFPRSPEEDQQAFRPLE KENQEPLRFEEAEDQVLERLIEKERQESLKSPEEEDQQAFRLLEKENQEPLRFEDAEDQVLERLIEKERQ ESLKSPEEEDQQAFRLLEKENQEPLRFEEAEDQVLERLVEKESQESLKSPEEEDQRTGKPLEKENQESLR SLDENQETIVLLESKNQRPLRSLEVEEEEQRIVKPLEKVSQVSLESLEKENVQSPRYLEEDDHMIKSLLE DKTHEILGSLEDRNGENFIPPENETQGSLRPPEEEDQRIVNHLEKESQEFLRSPEAEEEEEQVMVRSLEG ENHDPLSSVVKEEQMAESKLENESQDSRKSLEDESQETFGSLEKENLESLRSLAGQDQEEQKLEQETQQP LRAVEDEQMTVNPPEKVDPELPKPLRNDQEVVRSLDKENQESLVSLNEGGMETVKSSETENIESLETVGE CLGRRKSVDTQEPLWSTEVTSETIEPLEDETQEPLGCVDENQEVLTPLERESQELRSLGKWNPETVESPG GVEDSQQCLEVEEGPEREQHQESLRSLGEVEWELPGSGSQQRWEDVVEDGEGQEASLGATGVETEDKAEL HLRGQGGEEKAVEEGELLQDAVGEAWSLGSSEPKEQRVPAEPLDDLEGQPEQTGTLEVPVAQGMPEATEQ DEDRAQAGEQDSVEVTLGLEAARAGLELEQEVVGLEDPRHFAREEAIHPSLGEESVKAKIDQGLEEPGKE PKEAGALDSGIPELPKTSSETLECKGWEESGEGWGEEEASLETSDHEGSHAPQPRPPKTEEDEGLQAALT VPGPKLLEPCSPIPILTDAHELQPQAEGIQEAGWQPEAGTEALGRVEDEPEFGRGEIPEGLQDWEEGRED SEADELGETLPDSTPLGLYLKSPASPKWEQAGEQRLFPQGEARKEGWSPAALAAQGLSDPPEEEQQGHDS DLSSEEFEDLGTEASLLPGVPKEVSDHLGQEPPVLQPACWDQGGESDGFADEEESGEEGEEEDADEEEGA ESGTQWWGPGPSGGGVKVQDVTQRGDLEHESVGDSGLWDDGLSGAAANVLVTALETVSQDSAEPSGSEGS ESASLEGEEGQAIDHLDAPQEVTSVVPGAGDTFDISGQGPNLESEQVNGRMENGLEQAEGQVVLHGDEDQ GIPLQEQGTLKAPLVGSPVHLGPSQPLKFTLSGVDGDSWSSGED TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016701 |
| ORF Size | 5592 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016701.3, NP_057910.3 |
| RefSeq Size | 6143 bp |
| RefSeq ORF | 5595 bp |
| Locus ID | 18008 |
| UniProt ID | Q6P5H2 |
| Gene Summary | Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division (By similarity). Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.[UniProtKB/Swiss-Prot Function] |
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